BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00002813
         (1324 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   701   0.0  
sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coc...   691   0.0  
gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltra...   621   e-175
dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransfe...   495   e-138
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau...   489   e-136

>gb|ABY75613.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  701 bits (1808), Expect = 0.0
 Identities = 349/350 (99%), Positives = 350/350 (100%)
 Frame = -3

Query: 1256 MAFHGKDDALDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP 1077
            MAFHGKDDALDIKAQAHVWKIIYGFADSLVLRCAVELGIVDI+DNNNQPMALADLASKLP
Sbjct: 1    MAFHGKDDALDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIVDNNNQPMALADLASKLP 60

Query: 1076 VSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQ 897
            VSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQ
Sbjct: 61   VSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQ 120

Query: 896  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 717
            KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS
Sbjct: 121  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 180

Query: 716  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 537
            GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM
Sbjct: 181  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 240

Query: 536  FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 357
            FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST
Sbjct: 241  FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 300

Query: 356  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 207
            RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP
Sbjct: 301  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350


>sp|Q9LEL5.1|4OMT_COPJA RecName: Full=3'-hydroxy-N-methyl-(S)-coclaurine
            4'-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:3'-hydroxy-N-
            methylcoclaurine 4'-O-methyltransferase; Short=4'-OMT
            gi|9711916|dbj|BAB08005.1|
            S-adenosyl-L-methionine:3'-hydroxy-N-methylcocla urine
            4'-O-methyltransferase [Coptis japonica]
          Length = 350

 Score =  691 bits (1784), Expect = 0.0
 Identities = 345/350 (98%), Positives = 348/350 (99%)
 Frame = -3

Query: 1256 MAFHGKDDALDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP 1077
            MAFHGKDD LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP
Sbjct: 1    MAFHGKDDVLDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLP 60

Query: 1076 VSNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQ 897
            VS+VNCDNLYRILRYLVKMEILRVEKSDDG+KKYALEPIATLLSRNAKRSMVPMILGMTQ
Sbjct: 61   VSDVNCDNLYRILRYLVKMEILRVEKSDDGQKKYALEPIATLLSRNAKRSMVPMILGMTQ 120

Query: 896  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 717
            KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS
Sbjct: 121  KDFMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 180

Query: 716  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 537
            GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYD PNIERIGGDM
Sbjct: 181  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDLPNIERIGGDM 240

Query: 536  FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 357
            FKSVPSAQAI+LKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST
Sbjct: 241  FKSVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 300

Query: 356  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 207
            RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP
Sbjct: 301  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 350


>gb|AAU20768.1| 3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase [Thalictrum
            flavum subsp. glaucum]
          Length = 348

 Score =  621 bits (1602), Expect = e-175
 Identities = 312/350 (89%), Positives = 326/350 (93%), Gaps = 1/350 (0%)
 Frame = -3

Query: 1253 AFHGKDDALDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPV 1074
            AF GKD ++DIKAQAHVWKIIYGFADSLVLRCAVELGI DIIDNNNQP+ LADLASKLP+
Sbjct: 3    AFQGKDASVDIKAQAHVWKIIYGFADSLVLRCAVELGIFDIIDNNNQPIPLADLASKLPI 62

Query: 1073 SNVNCDNLYRILRYLVKMEILRVEKSDDGEKKYALEPIATLLSRNAKRSMVPMILGMTQK 894
            SNVN DNL+RILRYLVKME+L     D    KYALEPIA L+ RN KRSMVPM+LGMTQK
Sbjct: 63   SNVNLDNLFRILRYLVKMELLSYAADD----KYALEPIAKLVLRNEKRSMVPMVLGMTQK 118

Query: 893  DFMTPWHSMKDGLSDNG-TAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 717
            DFMTPWHSMKDGL+DNG TAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS
Sbjct: 119  DFMTPWHSMKDGLTDNGVTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLIS 178

Query: 716  GSRDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 537
            GS+DMFQ IDSLVDVGGGNGTT+KAIS AFPHIKCTLFDLPHVIANSYDHPNIERIGGDM
Sbjct: 179  GSKDMFQSIDSLVDVGGGNGTTIKAISVAFPHIKCTLFDLPHVIANSYDHPNIERIGGDM 238

Query: 536  FKSVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSST 357
            FK +PSAQAILLKLILHDWNDEDSIKILKQCR AVPKDGGKVIIVDVALDE+SDHELSST
Sbjct: 239  FKGMPSAQAILLKLILHDWNDEDSIKILKQCRKAVPKDGGKVIIVDVALDEDSDHELSST 298

Query: 356  RLILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 207
            RLILDIDMLVNTGGKERTKE WEK+VK AGFSGCKIRHIAAIQSVIEVFP
Sbjct: 299  RLILDIDMLVNTGGKERTKEDWEKLVKCAGFSGCKIRHIAAIQSVIEVFP 348


>dbj|BAM37633.1| 3'-hydroxy-N-methylcoclaurine-4'-O-methyltransferase [Eschscholzia
            californica]
          Length = 353

 Score =  495 bits (1275), Expect = e-138
 Identities = 238/348 (68%), Positives = 297/348 (85%), Gaps = 4/348 (1%)
 Frame = -3

Query: 1238 DDALDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSNVNC 1059
            ++ +DIKAQAH+W IIYGFADSLVLR AVELGI DII NNN  + +++LASKLP+SNVN 
Sbjct: 6    NEEVDIKAQAHLWNIIYGFADSLVLRSAVELGIADIIKNNNGSITVSELASKLPISNVNS 65

Query: 1058 DNLYRILRYLVKMEILRVEKS--DDGE--KKYALEPIATLLSRNAKRSMVPMILGMTQKD 891
            DNLYR+LRYLV M IL+  KS  + GE  K Y+LEP+ +LL ++A+R+MVP++LGMTQ+D
Sbjct: 66   DNLYRVLRYLVHMGILKETKSTINGGEIKKLYSLEPVGSLLVKDAERNMVPIVLGMTQQD 125

Query: 890  FMTPWHSMKDGLSDNGTAFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGS 711
            FM PWH +K+GL +  TAFEK MGMT+WEYLEGHP+Q  LFN GM GETRLLT +LI   
Sbjct: 126  FMIPWHYIKEGLGEGSTAFEKGMGMTLWEYLEGHPEQGHLFNVGMEGETRLLTKTLIESC 185

Query: 710  RDMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFK 531
            +D F+G+ SLVDVGGGNGTT+KAIS+AFPHIKC+L+DLPHV+A+S+D PNIE+I GD+FK
Sbjct: 186  KDTFEGLSSLVDVGGGNGTTIKAISEAFPHIKCSLYDLPHVVADSHDLPNIEKIPGDIFK 245

Query: 530  SVPSAQAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRL 351
             +P+AQAILLKLILHDW+DEDS+KILK+CR AVP+D G+VIIVDVAL+EES+H L+ TRL
Sbjct: 246  FIPNAQAILLKLILHDWSDEDSVKILKKCREAVPQDTGRVIIVDVALEEESEHPLTKTRL 305

Query: 350  ILDIDMLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 207
            +LD+DMLVNTGG+ER+++ W K++K AGF   KIRHIAA+QSVIE FP
Sbjct: 306  VLDVDMLVNTGGRERSEDDWAKLLKLAGFRTHKIRHIAAVQSVIEAFP 353


>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
            4'-O-methyltransferase 2 [Papaver somniferum]
          Length = 357

 Score =  489 bits (1258), Expect = e-136
 Identities = 241/343 (70%), Positives = 287/343 (83%), Gaps = 2/343 (0%)
 Frame = -3

Query: 1229 LDIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSNVNCDNL 1050
            + IK QA +W IIYGFADSLVLRCAVE+GI DII NN+  + LA LA+KLP++NV+ D L
Sbjct: 15   VSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSDYL 74

Query: 1049 YRILRYLVKMEILRVEKSDDG-EKKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTPWH 873
            YR++RYLV + I+  E  + G EK Y+L+P+ TLL R+A+RSMVPMILGMTQKDFM  WH
Sbjct: 75   YRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH 134

Query: 872  SMKDGLSDNGT-AFEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSLISGSRDMFQ 696
             MK+GL +  T AFEK MGM IW+YLEG+PDQSQLFNEGMAGETRLLT +LI   RD FQ
Sbjct: 135  FMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDTFQ 194

Query: 695  GIDSLVDVGGGNGTTVKAISDAFPHIKCTLFDLPHVIANSYDHPNIERIGGDMFKSVPSA 516
            G+DSLVD+GGGNGTT+KAI +AFPHIKCTL+DLPHV+ANS+D PNIE++ GDMFKSVPSA
Sbjct: 195  GLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVPSA 254

Query: 515  QAILLKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRLILDID 336
            QAILLKLILHDW DE+ + ILK+C+ A+PK+ GKVIIVDVAL+EES+HEL+ TRLILDID
Sbjct: 255  QAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLILDID 314

Query: 335  MLVNTGGKERTKEVWEKIVKSAGFSGCKIRHIAAIQSVIEVFP 207
            MLVNTGG+ERT + WE ++K AGF   KIR I AIQSVIE FP
Sbjct: 315  MLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357