BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00002377
         (2131 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]       703   0.0  
gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver...   545   e-152
dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]    522   e-145
ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinif...   504   e-140
gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]           499   e-139

>dbj|BAF98472.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 534

 Score =  703 bits (1815), Expect = 0.0
 Identities = 351/532 (65%), Positives = 408/532 (76%)
 Frame = -1

Query: 2104 PQLHQWLPTSIIAPAPIVFSFIVIVLICIVKKRSGNVSKTRKAPEVSGAWPLVGHLNLVS 1925
            P L QW+ TS + PAPI+ SFIVI+L    KKRS N+ +T+KAPEV GAWP++GHLNL+S
Sbjct: 4    PTLKQWIRTSTL-PAPIIVSFIVILLFYFFKKRSSNMIRTKKAPEVVGAWPVIGHLNLLS 62

Query: 1924 GPKLVHFVLGELTDKYGPAFTIHLGVYPTFVVSNWELVKECFTTNDKIFSNRPVNKAIKY 1745
             PK  + VLGEL D+YGPAF+I  GV+P  VVS+WELVK CFTTNDK FS+R VNKAIKY
Sbjct: 63   VPKPAYIVLGELADQYGPAFSIQFGVHPILVVSSWELVKACFTTNDKFFSSRLVNKAIKY 122

Query: 1744 MFYNEESIGFTPYGSYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNK 1565
            MFY++++I F P+G YWRELRKM TL LLSN RL MLK  RISEMDAC + LYEL TK K
Sbjct: 123  MFYDQKTISFAPHGPYWRELRKMITLNLLSNERLKMLKHQRISEMDACLKKLYELSTKRK 182

Query: 1564 DDNVWVLVDMSKWFGEISFNVVARIVAGKKNFGSKGDRYKKVMEEVVRLMCVTALSDIVP 1385
            D+N  VLVDMSKWFG+ISFNVV RIVAGK  FG K +RY  VMEE  RLM V   SD++P
Sbjct: 183  DENAGVLVDMSKWFGDISFNVVTRIVAGKHIFGPKTERYMNVMEEARRLMDVMVFSDVIP 242

Query: 1384 YXXXXXXXXXXXRAMKRAAKELDSVLQSWVEEHRQRRVSVSAGTGSTANKTKEEVEKDFI 1205
            Y             +KR AKELDS L+SWV+EHRQ+RVS+SAG G   N T+EE E DFI
Sbjct: 243  YLGWLDRLRGVDSEIKRTAKELDSALESWVDEHRQKRVSISAGIGGIVNITEEE-EIDFI 301

Query: 1204 DITLSIMEENQLPGDDPDTVIKSLILDMILGGSDXXXXXXXXXXXXXXXXVHALKKAQRE 1025
            DI LSI+ +N+L GDDP T+IK+++ +M L   D                   LK+AQ E
Sbjct: 302  DIMLSIIAKNKLLGDDPGTLIKAIVQEMYLAAWDNTTVTLTWALCLLLNNKQVLKRAQCE 361

Query: 1024 LDAQIGKERQVEDSDIKNLPYIQAIIKEAMRLYPAGPIIEREASEDCDVGGFHVPAGTRL 845
            LDAQ+GKERQVEDSDI  LPYIQAI+KE+MRLYP GPIIERE +EDCDVG F +PAGTRL
Sbjct: 362  LDAQVGKERQVEDSDINTLPYIQAIVKESMRLYPPGPIIERETTEDCDVGDFRIPAGTRL 421

Query: 844  MVNLWKLQRDPNVWPNDPLEFRPERFLTSHADVDLRGQHLELIPFGSGRRVCPGISFSLQ 665
             +NLWKLQRDPNVWPNDP EF+PERFL  HAD+D++GQH ELIPFGSGRR+CPG+SFSLQ
Sbjct: 422  WINLWKLQRDPNVWPNDPQEFQPERFLNGHADIDMKGQHFELIPFGSGRRMCPGVSFSLQ 481

Query: 664  VMHLALARIIHGFELKIPNDSTIDMSGTPGIISCKATPLQVLLTARFNPVFY 509
            VMHL LARIIHGFELK P D+ IDMS T G+IS KATPL+VLLT RF PVFY
Sbjct: 482  VMHLVLARIIHGFELKTPTDADIDMSTTLGMISWKATPLEVLLTPRFPPVFY 533


>gb|AGC92398.1| (S)-cis-N-methylstylopine 14-hydroxylase [Papaver somniferum]
          Length = 526

 Score =  545 bits (1403), Expect = e-152
 Identities = 272/517 (52%), Positives = 360/517 (69%), Gaps = 6/517 (1%)
 Frame = -1

Query: 2059 PIVFSFIVIVLICIVKKRSGNVSKTRK-APEVSGAWPLVGHLNLVSGPKLVHFVLGELTD 1883
            PI  + +VI L+    +R+     T+K APE SG  P++GHL+L +  +L H  LG + D
Sbjct: 21   PISVALVVIALVWNYGRRN----PTKKLAPEASGGRPIMGHLHLFNDGELTHRKLGAMAD 76

Query: 1882 KYGPAFTIHLGVYPTFVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEESIGFTPYG 1703
             YGP F I  G + T VVS+WE+VKECFTTNDK+FSNRP    IK MFY+ +S+G+ PYG
Sbjct: 77   TYGPVFNIRFGSHKTLVVSDWEIVKECFTTNDKLFSNRPGTLGIKLMFYDADSVGYAPYG 136

Query: 1702 SYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWVLVDMSKWF 1523
            +YWR+LRK++TLKLLSNHR+D +K LR SE+++CF +LY  W   +    +  V M  W 
Sbjct: 137  AYWRDLRKISTLKLLSNHRIDTIKHLRSSEVESCFESLYSQWGNGEKSGEFAPVRMDSWL 196

Query: 1522 GEISFNVVARIVAGKKNFGSKGD----RYKKVMEEVVRLMCVTALSDIVPYXXXXXXXXX 1355
            G+++FNVVARIVAGKKNF + GD    RYK  M+E +RLM   A SD++P          
Sbjct: 197  GDLTFNVVARIVAGKKNFSANGDVGAQRYKAAMDEAMRLMRFFAFSDVIPSLSWLDNLRG 256

Query: 1354 XXRAMKRAAKELDSVLQSWVEEHRQRRVSVSAGTGSTANKTKEEVEKDFIDITLSIMEEN 1175
              R MK+ A E+DS++ +WVEEHR +R   ++G  S       ++E DFID+ L IME +
Sbjct: 257  LVREMKKCASEIDSIMATWVEEHRVKR---NSGGNS-------QLEHDFIDVCLDIMEHS 306

Query: 1174 QLPGDDPDTVIKSLILDMILGGSDXXXXXXXXXXXXXXXXVHALKKAQRELDAQIGKERQ 995
             LPGDDPD V+KS  LDMILGGSD                   L+KA+ EL+ Q+GK RQ
Sbjct: 307  SLPGDDPDLVVKSTCLDMILGGSDTTTVTLTWAMSLLLNHPQVLQKAKEELETQVGKNRQ 366

Query: 994  VEDSDIKNLPYIQAIIKEAMRLYPAGPIIEREASEDCDVGGFHVPAGTRLMVNLWKLQRD 815
            V+DSDI NLP+IQAIIKE MRLYPAGP+IER   EDC+V G+ VPAGTRL+VN+WK+QRD
Sbjct: 367  VDDSDIPNLPFIQAIIKETMRLYPAGPLIERRTMEDCEVAGYQVPAGTRLLVNVWKMQRD 426

Query: 814  PNVWPNDPLEFRPERFLTSHADVDLRGQHLELIPFGSGRRVCPGISFSLQVMHLALARII 635
             NV+  DPLEFRP+RFLTS+ADVDL+GQH ELIPFG+GRR+CPG+SF++Q+MHL LAR++
Sbjct: 427  GNVYKGDPLEFRPDRFLTSNADVDLKGQHYELIPFGAGRRICPGVSFAVQLMHLVLARLL 486

Query: 634  HGFEL-KIPNDSTIDMSGTPGIISCKATPLQVLLTAR 527
            H FE+  +  ++ +DM+ + G++  K  PL+VL+  R
Sbjct: 487  HEFEITTVEPETKVDMAESGGLLCYKIMPLEVLIKPR 523


>dbj|BAK20464.1| protopine 6-hydroxylase [Eschscholzia californica]
          Length = 524

 Score =  522 bits (1345), Expect = e-145
 Identities = 260/534 (48%), Positives = 358/534 (67%), Gaps = 8/534 (1%)
 Frame = -1

Query: 2086 LPTSIIAPAPIVFSFIVIVLICIVKKRSGNVSKTRKAPEVSGAWPLVGHLNLVSGPKLVH 1907
            L  + + P  +      + L  +   R+    K R AP  +GAWP++GHL+L    +L H
Sbjct: 4    LMLAYLFPISVASIIAFVFLYNLFSSRTLKNKKIRTAPMATGAWPVLGHLHLFGSGELPH 63

Query: 1906 FVLGELTDKYGPAFTIHLGVYPTFVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEE 1727
             +L  + DKYG AF +  G + T VVS+  +VKECFTTND +FSNRP  KA + M Y+ E
Sbjct: 64   KMLAAMADKYGSAFRMKFGKHTTLVVSDTRIVKECFTTNDTLFSNRPSTKAFQLMTYDNE 123

Query: 1726 SIGFTPYGSYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWV 1547
            S+ FTPYGSYWRE+RK++TLKLLSNHRL  +K +R SE++ CF+ LY+   KN   +  +
Sbjct: 124  SVAFTPYGSYWREIRKISTLKLLSNHRLQAIKDVRASEVNVCFKTLYD-QCKNPSGSAPI 182

Query: 1546 LVDMSKWFGEISFNVVARIVAGKKNFGSK-------GDRYKKVMEEVVRLMCVTALSDIV 1388
            L+DM KWF E+S NVV R++ G++NFGSK          YKKVM+E++RL  ++  SD  
Sbjct: 183  LIDMKKWFEEVSNNVVMRVIVGRQNFGSKIVQGEEEAIHYKKVMDELLRLASLSMFSDFA 242

Query: 1387 PYXXXXXXXXXXXRAMKRAAKELDSVLQSWVEEHRQRRVSVSAGTGSTANKTKEEVEKDF 1208
            P             AMKR AK++D++L++W+EEHR+++ SV+            E ++DF
Sbjct: 243  PLLGFVDIFQGNLSAMKRNAKKVDAILENWLEEHRKKKNSVA------------ESQQDF 290

Query: 1207 IDITLSIMEENQLPGDDPDTVIKSLILDMILGGSDXXXXXXXXXXXXXXXXVHALKKAQR 1028
            +D+ LSI+EE++L G D D VIK+  L MI+GG+D                 HALKKA+ 
Sbjct: 291  MDVMLSIVEESKLSGHDADAVIKATCLAMIMGGTDTTAVSLTWIISLLMNNRHALKKARE 350

Query: 1027 ELDAQIGKERQVEDSDIKNLPYIQAIIKEAMRLYPAGPIIEREASEDCDVGGFHVPAGTR 848
            ELDA +GK+RQVEDSD+KNL Y+ AI+KE MR+YP G ++ERE  EDC++ GFHV  GTR
Sbjct: 351  ELDALVGKDRQVEDSDLKNLVYMNAIVKETMRMYPLGTLLERETKEDCEIDGFHVKGGTR 410

Query: 847  LMVNLWKLQRDPNVWPNDPLEFRPERFLTSHADVDLRGQHLELIPFGSGRRVCPGISFSL 668
            L+VN+WKLQRDPNVW  DP EFRPERFLT +AD+D+ GQH EL+PFG+GRRVCPG+ F+L
Sbjct: 411  LLVNVWKLQRDPNVWV-DPTEFRPERFLTENADIDVGGQHFELLPFGAGRRVCPGVXFAL 469

Query: 667  QVMHLALARIIHGFELKIPNDSTIDMSGTP-GIISCKATPLQVLLTARFNPVFY 509
            Q MHL LAR+IHG++L   N+  +D++ +P G ++ KA+PL ++LT R +   Y
Sbjct: 470  QFMHLVLARLIHGYDLNTLNEENVDLTESPEGHVNHKASPLDLILTPRLHYKLY 523


>ref|XP_002284031.1| PREDICTED: cytochrome P450 82C4 [Vitis vinifera]
            gi|302142392|emb|CBI19595.3| unnamed protein product
            [Vitis vinifera]
          Length = 527

 Score =  504 bits (1299), Expect = e-140
 Identities = 269/531 (50%), Positives = 354/531 (66%), Gaps = 12/531 (2%)
 Frame = -1

Query: 2065 PAPIVFSFIVIVLICIV-KKRSGNVSKTRKAPEVSGAWPLVGHLNLVSG-PKLVHFVLGE 1892
            PA  VF  ++ V   ++ +K+    SK+R APE  GAWP++GHL+L+ G  +L++  LG 
Sbjct: 8    PAFFVFLSLIYVFYAMLGRKKIIKSSKSRDAPEPGGAWPIIGHLHLLGGGDQLLYRTLGA 67

Query: 1891 LTDKYGPAFTIHLGVYPTFVVSNWELVKECFTTNDKIFSNRPVNKAIKYMFYNEESIGFT 1712
            + DKYGPAF I LG    FVVS+WE+ KECFT NDK  + RP   A K+M YN    GF 
Sbjct: 68   MADKYGPAFNIRLGSRRAFVVSSWEVAKECFTINDKALATRPTTVAAKHMGYNYAVFGFA 127

Query: 1711 PYGSYWRELRKMTTLKLLSNHRLDMLKPLRISEMDACFRNLYELWTKNKDDNVWVLVDMS 1532
            PY  +WRE+RK+ TL+LLSN RL++LK +R SE+D   R LY LW KN      +LV+++
Sbjct: 128  PYSPFWREMRKIATLELLSNRRLEILKHVRTSEVDMGIRELYGLWVKNSSRP--LLVELN 185

Query: 1531 KWFGEISFNVVARIVAGKKNFG-------SKGDRYKKVMEEVVRLMCVTALSDIVPYXXX 1373
            +W  +++ NV+ R+VAGK+ FG       S+  R +K + +  RL+ +  +SD +P+   
Sbjct: 186  RWLEDMTLNVIVRMVAGKRYFGAAAASDSSEARRCQKAISQFFRLIGIFVVSDALPF-LW 244

Query: 1372 XXXXXXXXRAMKRAAKELDSVLQSWVEEHRQRRVSVSAGTGSTANKTKEEVEKDFIDITL 1193
                    RAMK  AKELDS+L+ W+EEHRQRRVS         +  K E E+DFID+ L
Sbjct: 245  WLDLQGHERAMKTTAKELDSILEGWLEEHRQRRVS---------SLIKAEGEQDFIDVML 295

Query: 1192 SIMEENQLPG--DDPDTVIKSLILDMILGGSDXXXXXXXXXXXXXXXXVHALKKAQRELD 1019
            S+ EE +L G   D +T IKS  L +ILGGSD                 HALKKAQ ELD
Sbjct: 296  SLQEEGRLSGFQYDSETSIKSTCLALILGGSDTTAGTLTWAISLLLNNRHALKKAQEELD 355

Query: 1018 AQIGKERQVEDSDIKNLPYIQAIIKEAMRLYPAGPII-EREASEDCDVGGFHVPAGTRLM 842
              +G ERQVE+SD+KNL Y+QAIIKE +RLYPAGP++  REA +DC V G++VPAGTRL+
Sbjct: 356  LCVGMERQVEESDVKNLVYLQAIIKETLRLYPAGPLLGPREALDDCTVAGYNVPAGTRLI 415

Query: 841  VNLWKLQRDPNVWPNDPLEFRPERFLTSHADVDLRGQHLELIPFGSGRRVCPGISFSLQV 662
            VN+WKLQRDP+VW N P  F+PERFL +HADVD++GQ  EL+PFGSGRR CPG+SF+LQV
Sbjct: 416  VNIWKLQRDPSVWTN-PCAFQPERFLNAHADVDVKGQQFELMPFGSGRRSCPGVSFALQV 474

Query: 661  MHLALARIIHGFELKIPNDSTIDMSGTPGIISCKATPLQVLLTARFNPVFY 509
            +HL LAR++H FEL  P D  +DM+ + G+   KATPL+VLLT R N   Y
Sbjct: 475  LHLTLARLLHAFELSTPVDQPVDMTESSGLTIPKATPLEVLLTPRLNSKLY 525


>gb|AGC92397.1| protopine 6-hydroxylase [Papaver somniferum]
          Length = 541

 Score =  499 bits (1286), Expect = e-139
 Identities = 256/555 (46%), Positives = 359/555 (64%), Gaps = 18/555 (3%)
 Frame = -1

Query: 2119 SSPMYPQLHQWLPTSIIAPAPIVFSFIVIVLICIVKKRSGNVSKTR---------KAPEV 1967
            SS +   L+ W+    +A      + + +VL+  ++    + SKT          + P V
Sbjct: 4    SSLLLLLLNTWISAYSMA------ALLALVLVYNLRMTKSSSSKTTSLKGKKIITRPPAV 57

Query: 1966 SGAWPLVGHLNLVSGPKLVHFVLGELTDKYGPAFTIHLGVYPTFVVSNWELVKECFTTND 1787
            +GAWP+ GHL+L    +  H +L +L +KYGP+FT+  G + T VVS+  +VKECFTTND
Sbjct: 58   TGAWPVFGHLHLFGSGEHPHEMLSKLAEKYGPSFTMKFGKHTTLVVSDTRVVKECFTTND 117

Query: 1786 KIFSNRPVNKAIKYMFYNEESIGFTPYGSYWRELRKMTTLKLLSNHRLDMLKPLRISEMD 1607
             +FSNRP   A   M Y  +SI FTPY  YWRELRK++TLKLLSN+RL+ +K LR SE+ 
Sbjct: 118  TLFSNRPSTIAFDLMTYATDSIAFTPYSPYWRELRKISTLKLLSNNRLESIKQLRTSEVS 177

Query: 1606 ACFRNLYELWTKNKDDNVWVLVDMSKWFGEISFNVVARIVAGKKNFGSK--------GDR 1451
             CF+ LY+L  K  D+   V +D+ +WF E+S NV+ R++ GK+NFGSK           
Sbjct: 178  VCFKELYDLTNKKNDNGAPVPIDLKRWFDEVSNNVIMRVIFGKQNFGSKIVLGEDQEAVH 237

Query: 1450 YKKVMEEVVRLMCVTALSDIVPYXXXXXXXXXXXRAMKRAAKELDSVLQSWVEEHRQRRV 1271
            YKK+M+E+ RL  +T LSD+VP            RAMKR  KEL+S+LQ W+EEH+ ++ 
Sbjct: 238  YKKIMDELSRLSSLTMLSDMVPLLGWLDYFKGDLRAMKRNGKELNSILQKWLEEHKSKKS 297

Query: 1270 SVSAGTGSTANKTKEEVEKDFIDITLSIMEENQLPGDDPDTVIKSLILDMILGGSDXXXX 1091
            S              +  +DF+D+ LSI ++ QL G D DT IK+  L MI+GG++    
Sbjct: 298  S--------------DARQDFMDVMLSISKDTQLYGHDQDTFIKATCLAMIMGGTNSTEV 343

Query: 1090 XXXXXXXXXXXXVHALKKAQRELDAQIGKERQVEDSDIKNLPYIQAIIKEAMRLYPAGPI 911
                          AL KA+ E+D  +GK+RQVEDSD+KNL Y+ AIIKE MRLYP G +
Sbjct: 344  ALTWILSLLMNNRCALHKAREEIDLLVGKDRQVEDSDVKNLTYMNAIIKETMRLYPLGFL 403

Query: 910  IEREASEDCDVGGFHVPAGTRLMVNLWKLQRDPNVWPNDPLEFRPERFLTSHADVDLRGQ 731
            +ER+  EDC+V GF++  GTRL++N+WKLQRDPNVW  DP+EF+PERFLT +AD+D+ GQ
Sbjct: 404  LERDTKEDCEVSGFNIKGGTRLLINVWKLQRDPNVW-TDPMEFKPERFLTENADIDVGGQ 462

Query: 730  HLELIPFGSGRRVCPGISFSLQVMHLALARIIHGFELKIPNDSTIDMS-GTPGIISCKAT 554
            H EL+PFG+GRRVCPG+SF+LQ MHL LAR+IHG++++  N   +D+S  + G ++ K+T
Sbjct: 463  HFELLPFGAGRRVCPGVSFALQFMHLVLARLIHGYDMETLNGEDVDLSVSSGGHVNIKST 522

Query: 553  PLQVLLTARFNPVFY 509
            PL+++LT R +P  Y
Sbjct: 523  PLELILTPRLHPELY 537