BLASTX nr result
ID: Coptis25_contig00002286
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00002286
(1730 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 850 0.0
emb|CBI20743.3| unnamed protein product [Vitis vinifera] 809 0.0
ref|XP_003522115.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 776 0.0
ref|XP_002308509.1| predicted protein [Populus trichocarpa] gi|2... 775 0.0
ref|NP_180194.2| 3beta-hydroxysteroid-dehydrogenase/decarboxylas... 774 0.0
>ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2-like [Vitis vinifera]
Length = 563
Score = 850 bits (2195), Expect = 0.0
Identities = 417/545 (76%), Positives = 483/545 (88%)
Frame = -3
Query: 1725 MTGEEKCCVVTGGRGFAARHLVEMLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSG 1546
M GE+K CVVTGGRGFAARHLVEMLI ++ V+IADLGPSI+LEP EE+ +L +AL+SG
Sbjct: 1 MEGEQKWCVVTGGRGFAARHLVEMLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSG 60
Query: 1545 AAKYVSADLRHKSQLIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECK 1366
A+YVSADLR K+Q++KA +GA+ VFHMAAPDSSINN QLH+SVNVQGTKNVIDAC+E K
Sbjct: 61 RAEYVSADLRDKAQVLKACQGAEAVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQK 120
Query: 1365 VKRLIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTC 1186
VKRLIYTSSPSVVFDGVHGIFNGDESL YP KHNDSYSATKAEGEALVIKSNG NGLLTC
Sbjct: 121 VKRLIYTSSPSVVFDGVHGIFNGDESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTC 180
Query: 1185 CIRPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSS 1006
CIRPSSIFGPGD+LLVPSLV AARAGKSKFIIGDGNN+YDFTYVENVAHAH+CAE AL+S
Sbjct: 181 CIRPSSIFGPGDKLLVPSLVNAARAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALAS 240
Query: 1005 EGEVAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYK 826
EG++A++AAGQAYFITNMEPIKFWEFVS ILEGLGYERP IKIP VMMPIAH+VEWTYK
Sbjct: 241 EGKIAEQAAGQAYFITNMEPIKFWEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYK 300
Query: 825 VLAPYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAE 646
+LAPYGM+VPQLTPSRIRLLS SRTFSCSKA+D+L Y PIVSLQ+GL+RTI+SY LRAE
Sbjct: 301 MLAPYGMKVPQLTPSRIRLLSCSRTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAE 360
Query: 645 QKQRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQI 466
+R GPSKAS YLG G++A+TLLWKDKK LT ++VL+AFY FIASGYTI+TAVS++
Sbjct: 361 HLPKREGPSKASRYLGSGRVADTLLWKDKKLALTTLLVLMAFYHNFIASGYTIVTAVSKL 420
Query: 465 LLMATLFLFVHGILPAKVLGYTIEKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLC 286
L+A++FLF+HG LP K+LGYT+EKIP SNFHF E+ SH+ ALS S+WN AVNVLKSLC
Sbjct: 421 FLVASIFLFIHGNLPEKILGYTVEKIPPSNFHFSEERSHRAALSVASSWNTAVNVLKSLC 480
Query: 285 KGEDFVLFVKVVISLLICSFLGATSIRSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALL 106
KG+D++LF+KVV+SLLI SFLGA S++S+F+ G+ AFVAF++YE KEEEID L +AL
Sbjct: 481 KGDDWMLFLKVVLSLLILSFLGAISLQSLFVTGLLIAFVAFYIYENKEEEIDRLASEALS 540
Query: 105 LGCKV 91
CK+
Sbjct: 541 FSCKL 545
>emb|CBI20743.3| unnamed protein product [Vitis vinifera]
Length = 540
Score = 809 bits (2090), Expect = 0.0
Identities = 397/522 (76%), Positives = 462/522 (88%)
Frame = -3
Query: 1656 MLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSGAAKYVSADLRHKSQLIKAFEGAD 1477
MLI ++ V+IADLGPSI+LEP EE+ +L +AL+SG A+YVSADLR K+Q++KA +GA+
Sbjct: 1 MLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSGRAEYVSADLRDKAQVLKACQGAE 60
Query: 1476 VVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVKRLIYTSSPSVVFDGVHGIFNG 1297
VFHMAAPDSSINN QLH+SVNVQGTKNVIDAC+E KVKRLIYTSSPSVVFDGVHGIFNG
Sbjct: 61 AVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQKVKRLIYTSSPSVVFDGVHGIFNG 120
Query: 1296 DESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTCCIRPSSIFGPGDRLLVPSLVTAA 1117
DESL YP KHNDSYSATKAEGEALVIKSNG NGLLTCCIRPSSIFGPGD+LLVPSLV AA
Sbjct: 121 DESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTCCIRPSSIFGPGDKLLVPSLVNAA 180
Query: 1116 RAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEGEVAQRAAGQAYFITNMEPIKF 937
RAGKSKFIIGDGNN+YDFTYVENVAHAH+CAE AL+SEG++A++AAGQAYFITNMEPIKF
Sbjct: 181 RAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALASEGKIAEQAAGQAYFITNMEPIKF 240
Query: 936 WEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVLAPYGMRVPQLTPSRIRLLSRS 757
WEFVS ILEGLGYERP IKIP VMMPIAH+VEWTYK+LAPYGM+VPQLTPSRIRLLS S
Sbjct: 241 WEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYKMLAPYGMKVPQLTPSRIRLLSCS 300
Query: 756 RTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQKQRRVGPSKASIYLGRGKIANT 577
RTFSCSKA+D+L Y PIVSLQ+GL+RTI+SY LRAE +R GPSKAS YLG G++A+T
Sbjct: 301 RTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAEHLPKREGPSKASRYLGSGRVADT 360
Query: 576 LLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQILLMATLFLFVHGILPAKVLGYTI 397
LLWKDKK LT ++VL+AFY FIASGYTI+TAVS++ L+A++FLF+HG LP K+LGYT+
Sbjct: 361 LLWKDKKLALTTLLVLMAFYHNFIASGYTIVTAVSKLFLVASIFLFIHGNLPEKILGYTV 420
Query: 396 EKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLCKGEDFVLFVKVVISLLICSFLGA 217
EKIP SNFHF E+ SH+ ALS S+WN AVNVLKSLCKG+D++LF+KVV+SLLI SFLGA
Sbjct: 421 EKIPPSNFHFSEERSHRAALSVASSWNTAVNVLKSLCKGDDWMLFLKVVLSLLILSFLGA 480
Query: 216 TSIRSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALLLGCKV 91
S++S+F+ G+ AFVAF++YE KEEEID L +AL CK+
Sbjct: 481 ISLQSLFVTGLLIAFVAFYIYENKEEEIDRLASEALSFSCKL 522
>ref|XP_003522115.1| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2-like [Glycine max]
Length = 563
Score = 776 bits (2005), Expect = 0.0
Identities = 386/544 (70%), Positives = 458/544 (84%), Gaps = 2/544 (0%)
Frame = -3
Query: 1716 EEKCCVVTGGRGFAARHLVEMLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSGAAK 1537
E+K CVVTGGRGFAARHLVEMLI ++ V+IADL +I LEP E+ +L QAL SG A+
Sbjct: 5 EDKWCVVTGGRGFAARHLVEMLIRHNEYCVRIADLEANIVLEPAEQLGLLGQALHSGRAQ 64
Query: 1536 YVSADLRHKSQLIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKVKR 1357
YVS DLR+K+QL+KA EG +VVFHMAAP+SSINN QLH+SVNVQGTKNVIDACVE VKR
Sbjct: 65 YVSLDLRNKAQLLKALEGVEVVFHMAAPNSSINNYQLHHSVNVQGTKNVIDACVELNVKR 124
Query: 1356 LIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTCCIR 1177
L+YTSSPSVVFDGVHGI NG+E++ Y ND YSATKAEGEALVIK+NG NGLLTCCIR
Sbjct: 125 LVYTSSPSVVFDGVHGIHNGNETMPYAHSPNDHYSATKAEGEALVIKANGTNGLLTCCIR 184
Query: 1176 PSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSEGE 997
PSSIFGPGDRLLVPSLV AAR GKSKF+IGDGNN+YDFTYVENVAHAH+CA+ AL SE
Sbjct: 185 PSSIFGPGDRLLVPSLVDAARKGKSKFLIGDGNNVYDFTYVENVAHAHICADRALVSEAP 244
Query: 996 VAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKVLA 817
V+++AAG+AYFITNMEP+KFWEFVS ++EGLGYE P IKIP V+MP AH+VEW Y++L
Sbjct: 245 VSEKAAGEAYFITNMEPMKFWEFVSVVVEGLGYEGPRIKIPTFVIMPFAHLVEWIYRLLG 304
Query: 816 PYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQ-- 643
PYGM+VPQLTPSRIRL S SRTF CSKA+DRLGY PIV+LQ+GL+RTIESY+HL+AE
Sbjct: 305 PYGMKVPQLTPSRIRLTSCSRTFDCSKAKDRLGYAPIVTLQEGLRRTIESYTHLKAENQP 364
Query: 642 KQRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQIL 463
K +R G SKASIYLG G++A+TLLWKDKKQT A++VLIA Y FIAS TII+A++++L
Sbjct: 365 KTKREGSSKASIYLGSGRVADTLLWKDKKQTFIALLVLIAIYFNFIASENTIISALTKLL 424
Query: 462 LMATLFLFVHGILPAKVLGYTIEKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLCK 283
L A++FLF+HGILPAK+LGYT+EKIP S FH E SHQ+ALS S+WN AVNVLKSL
Sbjct: 425 LFASIFLFIHGILPAKILGYTLEKIPKSWFHLSEDMSHQIALSVASSWNIAVNVLKSLAD 484
Query: 282 GEDFVLFVKVVISLLICSFLGATSIRSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALLL 103
G D+VLF K V+SL I SFLGA S++S++ IG+ FAF+AF++YE+KEEEI GL + L
Sbjct: 485 GNDWVLFFKAVLSLFILSFLGAFSLQSLYTIGVTFAFIAFYVYEQKEEEIGGLFIKTHSL 544
Query: 102 GCKV 91
GCK+
Sbjct: 545 GCKL 548
>ref|XP_002308509.1| predicted protein [Populus trichocarpa] gi|222854485|gb|EEE92032.1|
predicted protein [Populus trichocarpa]
Length = 558
Score = 775 bits (2000), Expect = 0.0
Identities = 388/545 (71%), Positives = 450/545 (82%)
Frame = -3
Query: 1725 MTGEEKCCVVTGGRGFAARHLVEMLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSG 1546
M+GEE+ CVVTGGRGFAARHLVEMLI + V+IADL P I L E+ L AL+SG
Sbjct: 1 MSGEERWCVVTGGRGFAARHLVEMLIQYDLFSVRIADLEPQITLSTEEQNGALGHALKSG 60
Query: 1545 AAKYVSADLRHKSQLIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECK 1366
A YVSADLR K Q++K EGA+VVFHMAAP+SS+N+QQLHYSVNVQGTKNVIDACVE K
Sbjct: 61 RANYVSADLRDKPQVLKVIEGAEVVFHMAAPNSSVNSQQLHYSVNVQGTKNVIDACVELK 120
Query: 1365 VKRLIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTC 1186
VKRLIYTSS SVVFDG+HGI NGDESL Y K DSYSATKAEGEA ++K+NG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGIHGIINGDESLPYTDKPLDSYSATKAEGEAAILKANGTNGLLTC 180
Query: 1185 CIRPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSS 1006
+RPSSIFGPGDRL VPSLV AARAGKSKF+IGDGNN+YDFTYVENVAHAH+CA+ AL+S
Sbjct: 181 SLRPSSIFGPGDRLFVPSLVAAARAGKSKFMIGDGNNIYDFTYVENVAHAHICADRALAS 240
Query: 1005 EGEVAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYK 826
E EVA++AAGQAYFITNMEPIKFWEF S ILEGLGYERP IKIP +MPIA V+EW YK
Sbjct: 241 EQEVAEKAAGQAYFITNMEPIKFWEFTSLILEGLGYERPRIKIPAFAVMPIAQVIEWIYK 300
Query: 825 VLAPYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAE 646
+ PYGM+VPQL PSRIRLLS SR+F+CSKA+DRLGY PIV L++GLKRT+ESY HLRAE
Sbjct: 301 IFGPYGMKVPQLIPSRIRLLSCSRSFNCSKAKDRLGYAPIVPLEEGLKRTLESYPHLRAE 360
Query: 645 QKQRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQI 466
+ +R GPSKASI LG GK +TLLWKDKKQTL ++VL+A Y FIAS TI+TA S++
Sbjct: 361 NQPKREGPSKASICLGSGK--DTLLWKDKKQTLITLLVLVAIYYNFIASQSTIVTAASKL 418
Query: 465 LLMATLFLFVHGILPAKVLGYTIEKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLC 286
LL+ ++FLF+HG LP KV GYTIEKIP S F+ E+ SHQ ALS S+WN AVNVLKSLC
Sbjct: 419 LLVVSVFLFIHGNLPEKVFGYTIEKIPMSYFNLSEERSHQTALSVASSWNAAVNVLKSLC 478
Query: 285 KGEDFVLFVKVVISLLICSFLGATSIRSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALL 106
KG D+ LF +VVISLLI SFLGA S +++F+IG+P AF AF+LYEK E+ ID + +AL
Sbjct: 479 KGNDWSLFFQVVISLLILSFLGAISFQTLFIIGLPVAFAAFYLYEKNEQAIDSMFLEALS 538
Query: 105 LGCKV 91
LGCK+
Sbjct: 539 LGCKM 543
>ref|NP_180194.2| 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
[Arabidopsis thaliana]
gi|229890388|sp|Q67ZE1.2|HSDD2_ARATH RecName:
Full=3beta-hydroxysteroid-dehydrogenase/decarboxylase
isoform 2; Short=At3BETAHSD/D2; AltName:
Full=4alpha-carboxysterol-C3-dehydrogenase/C4-
decarboxylase isoform 1-2; AltName: Full=Reticulon-like
protein B19; Short=AtRTNLB19; AltName:
Full=Sterol-4-alpha-carboxylate 3-dehydrogenase 2,
decarboxylating gi|330252720|gb|AEC07814.1|
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2 [Arabidopsis thaliana]
Length = 564
Score = 774 bits (1998), Expect = 0.0
Identities = 378/543 (69%), Positives = 452/543 (83%)
Frame = -3
Query: 1722 TGEEKCCVVTGGRGFAARHLVEMLIPCKKWLVKIADLGPSIQLEPYEEQSILAQALRSGA 1543
T E+ CVVTGGRGFAARHLVEML+ + + V+IADL P+I L+P E +L + LRSG
Sbjct: 6 TETERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGR 65
Query: 1542 AKYVSADLRHKSQLIKAFEGADVVFHMAAPDSSINNQQLHYSVNVQGTKNVIDACVECKV 1363
+Y+SADLR KSQ++KAF+GA+VVFHMAAPDSSINN QL YSVNVQGT+NVIDACV+ V
Sbjct: 66 VQYISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGV 125
Query: 1362 KRLIYTSSPSVVFDGVHGIFNGDESLAYPSKHNDSYSATKAEGEALVIKSNGRNGLLTCC 1183
KRLIYTSSPSVVFDGVHGI NG ES+AYP KHNDSYSATKAEGE L++K+NGRNGLLTCC
Sbjct: 126 KRLIYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCC 185
Query: 1182 IRPSSIFGPGDRLLVPSLVTAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAEHALSSE 1003
IRPSSIFGPGDRLLVPSLV AARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAE AL+S
Sbjct: 186 IRPSSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASG 245
Query: 1002 GEVAQRAAGQAYFITNMEPIKFWEFVSRILEGLGYERPSIKIPVPVMMPIAHVVEWTYKV 823
G+V+ +AAGQAYFITNMEPIKFWEF+S++L+GLGYERPSIKIP +MMPIAH+VE TYKV
Sbjct: 246 GDVSTKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKV 305
Query: 822 LAPYGMRVPQLTPSRIRLLSRSRTFSCSKARDRLGYEPIVSLQDGLKRTIESYSHLRAEQ 643
L PYGM VPQLTPSR+RLLS SRTF +KA+DRLGY P+V LQ+G++RTI+S+SHL A
Sbjct: 306 LGPYGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTAGS 365
Query: 642 KQRRVGPSKASIYLGRGKIANTLLWKDKKQTLTAVVVLIAFYSYFIASGYTIITAVSQIL 463
+ +R GPSKAS LG GK+A+TLLWKD KQTL A+ +LI+ Y F+A+G T++TA+S+ L
Sbjct: 366 QSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKAL 425
Query: 462 LMATLFLFVHGILPAKVLGYTIEKIPASNFHFPEKTSHQVALSAGSTWNYAVNVLKSLCK 283
L+A++FLF+HGILP K+ GYT+EKIPAS FH + +SH ++LS S+WN V L+SLC+
Sbjct: 426 LVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQ 485
Query: 282 GEDFVLFVKVVISLLICSFLGATSIRSIFMIGMPFAFVAFFLYEKKEEEIDGLVHQALLL 103
G D+ F KVV LL S GA S+ SIF+IG+P AF+AF +YEKKE+EID +V
Sbjct: 486 GNDWSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSIVVSFKSF 545
Query: 102 GCK 94
CK
Sbjct: 546 ACK 548