BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00002255
         (1616 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272876.2| PREDICTED: translation initiation factor eIF...   641   0.0  
ref|XP_002297747.1| predicted protein [Populus trichocarpa] gi|2...   625   e-176
gb|AAD52847.2|AF137288_1 putative translation initiation factor ...   625   e-176
ref|XP_004143752.1| PREDICTED: translation initiation factor eIF...   616   e-174
ref|XP_002304788.1| predicted protein [Populus trichocarpa] gi|2...   615   e-174

>ref|XP_002272876.2| PREDICTED: translation initiation factor eIF-2B subunit beta-like
            isoform 1 [Vitis vinifera]
            gi|359487220|ref|XP_003633538.1| PREDICTED: translation
            initiation factor eIF-2B subunit beta-like isoform 2
            [Vitis vinifera] gi|297742689|emb|CBI35142.3| unnamed
            protein product [Vitis vinifera]
          Length = 417

 Score =  641 bits (1654), Expect = 0.0
 Identities = 327/415 (78%), Positives = 358/415 (86%)
 Frame = -3

Query: 1530 MPDTHALVNEFVLMLKRRKVEGSQATAKQTAELLRSVISQQRVPHTNQAGALIDAINSVG 1351
            MPD   LVNEF++ LK+RKVEGSQATA+ TAELLRSVISQQRVP+ NQAGALIDAI +VG
Sbjct: 1    MPDVQVLVNEFIIKLKKRKVEGSQATARMTAELLRSVISQQRVPYNNQAGALIDAIKAVG 60

Query: 1350 EKLIAANPVELAVGNVVRRVLHIIREEDLSLATTAIGNVGLSAGSDDEDNVEQDDYPXXX 1171
            E+LIAANPVELAVGN+VRRVLHIIREEDLSLAT A+  + LSA SDDED+ E++D+P   
Sbjct: 61   EQLIAANPVELAVGNIVRRVLHIIREEDLSLATAAVAGLNLSAVSDDEDDGEREDHPVLS 120

Query: 1170 XXXXXXXXXXXXXAPSLHTLLEGIPDPAAAHRTXXXXXXXXXXXXXXXXSLRTRKLKHDV 991
                          PSL TLLE +PD AA  ++                S R+RKLKHD+
Sbjct: 121  AAAVAAAARSTLRPPSLQTLLEDMPDSAAVPQSSSSGGDSEGKSKSADKSSRSRKLKHDI 180

Query: 990  IEAVNELIQDIDTCHELIAEQAVEHIHHNEVILTLGRSRTVMEFLCAAKEKKRSFRVFVA 811
            IEAVNELIQDI TCHE I+EQAVEHIH NEVILTLGRSRTVMEFLCAAKEKKRSFRVFVA
Sbjct: 181  IEAVNELIQDIATCHEQISEQAVEHIHQNEVILTLGRSRTVMEFLCAAKEKKRSFRVFVA 240

Query: 810  EGAPRYQGHILAKELVARGLQTTVITDSAVFAMISRVNMVIVGAHAIMANGGVIAPVGLN 631
            EGAPRYQGH+LAKELVARGLQTT+ITDSAVFAMISRVNMVIVGAHA+MANGGVIAPVGLN
Sbjct: 241  EGAPRYQGHVLAKELVARGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVIAPVGLN 300

Query: 630  MVALAAQRHAVPFVVVAGSHKLCPLYPHNPEVLLNDLRSPSELLDFGEFSDCMDFGSGNG 451
            MVALAAQRHAVPFVV+AG+HKLCPLYPHNPEVLLN+L+SPSELLDFGEFSDCMDFGSGNG
Sbjct: 301  MVALAAQRHAVPFVVLAGTHKLCPLYPHNPEVLLNELKSPSELLDFGEFSDCMDFGSGNG 360

Query: 450  SPLLHVVNPAFDYVPPKLVTLFITDTGGHNPSYMYRLIADYYSADDFVVQRKSSA 286
            SPLLHVVNPAFDYVPPKLV+LFITDTGGHNPSYMYRLIADYYSADD VVQR+ ++
Sbjct: 361  SPLLHVVNPAFDYVPPKLVSLFITDTGGHNPSYMYRLIADYYSADDLVVQRRPAS 415


>ref|XP_002297747.1| predicted protein [Populus trichocarpa] gi|222845005|gb|EEE82552.1|
            predicted protein [Populus trichocarpa]
          Length = 419

 Score =  625 bits (1612), Expect = e-176
 Identities = 322/414 (77%), Positives = 353/414 (85%), Gaps = 2/414 (0%)
 Frame = -3

Query: 1530 MPDTHALVNEFVLMLKRRKVEGSQATAKQTAELLRSVISQQRVPHTNQAGALIDAINSVG 1351
            MPD  ALVN+F++ LK+RK+EGSQATA+ TAELLRSVISQQRVP+TNQAGALIDA+ +VG
Sbjct: 1    MPDVQALVNDFLIKLKKRKIEGSQATARHTAELLRSVISQQRVPYTNQAGALIDAVRAVG 60

Query: 1350 EKLIAANPVELAVGNVVRRVLHIIREEDLSLATTAIGNVGLSAGSD--DEDNVEQDDYPX 1177
            EKLIAANPVELAVGN+VRRVLHIIREEDLSL T AI  + LSA SD  D+D+ E+D++  
Sbjct: 61   EKLIAANPVELAVGNIVRRVLHIIREEDLSLTTAAIAGLNLSAASDGDDDDDGERDEHTV 120

Query: 1176 XXXXXXXXXXXXXXXAPSLHTLLEGIPDPAAAHRTXXXXXXXXXXXXXXXXSLRTRKLKH 997
                            PSL TLLE +P+ AA  RT                S RTRKLKH
Sbjct: 121  LSAAVVAAAARSTLCPPSLQTLLEDMPESAAIPRTSSSGGDSEGKSKSADKSSRTRKLKH 180

Query: 996  DVIEAVNELIQDIDTCHELIAEQAVEHIHHNEVILTLGRSRTVMEFLCAAKEKKRSFRVF 817
            DVIEAVNELIQDI TCHE IAEQA+EHIH NEVILTLG S+TV+EFL AAKEKKRSFRVF
Sbjct: 181  DVIEAVNELIQDITTCHEQIAEQAIEHIHQNEVILTLGSSKTVLEFLYAAKEKKRSFRVF 240

Query: 816  VAEGAPRYQGHILAKELVARGLQTTVITDSAVFAMISRVNMVIVGAHAIMANGGVIAPVG 637
            VAEGAPRYQGH+LAKELVARGLQTT+ITDSAVFA+ISRVNMVIVGAHA+MANGGVIAPVG
Sbjct: 241  VAEGAPRYQGHLLAKELVARGLQTTLITDSAVFAIISRVNMVIVGAHAVMANGGVIAPVG 300

Query: 636  LNMVALAAQRHAVPFVVVAGSHKLCPLYPHNPEVLLNDLRSPSELLDFGEFSDCMDFGSG 457
            LNMVALAAQ+HAVPFVV+AGSHKLCPLYPHNPEVLLN+LRSPSELLDFGEFSDC+DFGSG
Sbjct: 301  LNMVALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCLDFGSG 360

Query: 456  NGSPLLHVVNPAFDYVPPKLVTLFITDTGGHNPSYMYRLIADYYSADDFVVQRK 295
             GSPLLHVVNPAFDYVPPKLV+LFITDTGGHNPSYMYRLIADYYSADD VVQR+
Sbjct: 361  TGSPLLHVVNPAFDYVPPKLVSLFITDTGGHNPSYMYRLIADYYSADDLVVQRR 414


>gb|AAD52847.2|AF137288_1 putative translation initiation factor 2B beta subunit [Nicotiana
            tabacum]
          Length = 415

 Score =  625 bits (1612), Expect = e-176
 Identities = 313/413 (75%), Positives = 350/413 (84%)
 Frame = -3

Query: 1530 MPDTHALVNEFVLMLKRRKVEGSQATAKQTAELLRSVISQQRVPHTNQAGALIDAINSVG 1351
            MPD + LVN+F++ LK+RK+EGS+ATAK TAE+LRS ISQQR+PHTNQA ALIDAI ++G
Sbjct: 1    MPDMNTLVNDFIIKLKKRKIEGSKATAKLTAEVLRSCISQQRLPHTNQAAALIDAIRTIG 60

Query: 1350 EKLIAANPVELAVGNVVRRVLHIIREEDLSLATTAIGNVGLSAGSDDEDNVEQDDYPXXX 1171
            EKLIAANPVELAVGN+VRRVLHIIREED+SL   A+G + + AGSDDED+ +QDD+P   
Sbjct: 61   EKLIAANPVELAVGNIVRRVLHIIREEDVSLTAAAVGGLAVLAGSDDEDDFKQDDHPDLS 120

Query: 1170 XXXXXXXXXXXXXAPSLHTLLEGIPDPAAAHRTXXXXXXXXXXXXXXXXSLRTRKLKHDV 991
                          PSL TLLE IP   AA  T                +  +RKLKH++
Sbjct: 121  AAAVAAASRSTLRPPSLQTLLEDIPQSTAAPHTSSSGGDSEGKSKSADKNTASRKLKHNI 180

Query: 990  IEAVNELIQDIDTCHELIAEQAVEHIHHNEVILTLGRSRTVMEFLCAAKEKKRSFRVFVA 811
            IEAVNELIQDI TCHE IAEQAVEHIHHNEVILTLG SRTVMEFLCAAKEKKRSFRVFVA
Sbjct: 181  IEAVNELIQDIATCHEQIAEQAVEHIHHNEVILTLGNSRTVMEFLCAAKEKKRSFRVFVA 240

Query: 810  EGAPRYQGHILAKELVARGLQTTVITDSAVFAMISRVNMVIVGAHAIMANGGVIAPVGLN 631
            EGAPRYQGH LAKELVARGLQTTVITDSA+FAMISRVNMV+VGAHA+MANGGVIAPVG+N
Sbjct: 241  EGAPRYQGHALAKELVARGLQTTVITDSAIFAMISRVNMVVVGAHAVMANGGVIAPVGMN 300

Query: 630  MVALAAQRHAVPFVVVAGSHKLCPLYPHNPEVLLNDLRSPSELLDFGEFSDCMDFGSGNG 451
            MVALAAQRHAVPFVV+AG+HKLCPLYPHNPEVLLN+L+SP+ELLDFGEFSDC+DFGS +G
Sbjct: 301  MVALAAQRHAVPFVVLAGTHKLCPLYPHNPEVLLNELKSPAELLDFGEFSDCLDFGSSSG 360

Query: 450  SPLLHVVNPAFDYVPPKLVTLFITDTGGHNPSYMYRLIADYYSADDFVVQRKS 292
            SPLLHVVNPAFDYVPP LV+LFITDTGGHNPSYMYRLIADYYSADDFVV++ S
Sbjct: 361  SPLLHVVNPAFDYVPPNLVSLFITDTGGHNPSYMYRLIADYYSADDFVVKQSS 413


>ref|XP_004143752.1| PREDICTED: translation initiation factor eIF-2B subunit beta-like
            [Cucumis sativus]
          Length = 416

 Score =  616 bits (1589), Expect = e-174
 Identities = 313/414 (75%), Positives = 347/414 (83%)
 Frame = -3

Query: 1530 MPDTHALVNEFVLMLKRRKVEGSQATAKQTAELLRSVISQQRVPHTNQAGALIDAINSVG 1351
            MPD  A+VN+FV+ L +RK+EGS ATAK TAELLRSVIS QR+P+TNQA ALIDA+ +VG
Sbjct: 1    MPDVQAIVNDFVIKLMKRKIEGSLATAKHTAELLRSVISAQRIPYTNQAAALIDAVKAVG 60

Query: 1350 EKLIAANPVELAVGNVVRRVLHIIREEDLSLATTAIGNVGLSAGSDDEDNVEQDDYPXXX 1171
            E+LIAANPVELAVGN+VRRVLHIIREEDLSL T +I  + LSA SDDED++E+DD P   
Sbjct: 61   EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTASIAGLSLSAVSDDEDDIEKDDRPVLS 120

Query: 1170 XXXXXXXXXXXXXAPSLHTLLEGIPDPAAAHRTXXXXXXXXXXXXXXXXSLRTRKLKHDV 991
                          PSL TLLE +P+  + H+T                S R+RKLKHDV
Sbjct: 121  AAAVAAAARSTLRPPSLQTLLEDVPNQTSVHQTSSSGGDSEGKSKSADKSSRSRKLKHDV 180

Query: 990  IEAVNELIQDIDTCHELIAEQAVEHIHHNEVILTLGRSRTVMEFLCAAKEKKRSFRVFVA 811
            IEAVNELIQDI TCHE IAEQAVEHIH NEVILTLG SRTV+EFLCAAKEKKRSFRVFVA
Sbjct: 181  IEAVNELIQDIATCHEQIAEQAVEHIHQNEVILTLGSSRTVLEFLCAAKEKKRSFRVFVA 240

Query: 810  EGAPRYQGHILAKELVARGLQTTVITDSAVFAMISRVNMVIVGAHAIMANGGVIAPVGLN 631
            EGAPRYQGH+LAKELV RGLQTT+ITDSAVFAMISRVNMVIVGAHA+MANGGV+APVGLN
Sbjct: 241  EGAPRYQGHLLAKELVTRGLQTTLITDSAVFAMISRVNMVIVGAHAVMANGGVMAPVGLN 300

Query: 630  MVALAAQRHAVPFVVVAGSHKLCPLYPHNPEVLLNDLRSPSELLDFGEFSDCMDFGSGNG 451
            MVALAA+RHAVPFVV+AGSHKLCPLYPHNPEVLLN+LRSPSELLDFGEFSDCMDFG+  G
Sbjct: 301  MVALAAKRHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCMDFGTCTG 360

Query: 450  SPLLHVVNPAFDYVPPKLVTLFITDTGGHNPSYMYRLIADYYSADDFVVQRKSS 289
            SPLLHVVNP FDYVPP LV+LFITDTGGHN SYMYRLIADYYSADD V++R S+
Sbjct: 361  SPLLHVVNPTFDYVPPSLVSLFITDTGGHNSSYMYRLIADYYSADDLVIKRSST 414


>ref|XP_002304788.1| predicted protein [Populus trichocarpa] gi|222842220|gb|EEE79767.1|
            predicted protein [Populus trichocarpa]
          Length = 420

 Score =  615 bits (1587), Expect = e-174
 Identities = 316/415 (76%), Positives = 349/415 (84%), Gaps = 3/415 (0%)
 Frame = -3

Query: 1530 MPDTHALVNEFVLMLKRRKVEGSQATAKQTAELLRSVISQQRVPHTNQAGALIDAINSVG 1351
            MPD  ALVN+F++ LK+RK+EGSQATA+Q AELLRSVISQQRVP+TNQAGALIDA+ +VG
Sbjct: 1    MPDVQALVNDFLIKLKKRKIEGSQATARQAAELLRSVISQQRVPYTNQAGALIDAVRAVG 60

Query: 1350 EKLIAANPVELAVGNVVRRVLHIIREEDLSLATTAIGNVGLSA---GSDDEDNVEQDDYP 1180
            E+LIAANPVELAVGN+VRRVLHIIREEDLSL T AI  + LSA   G DD+D+ E+D++ 
Sbjct: 61   EQLIAANPVELAVGNIVRRVLHIIREEDLSLTTAAISGLNLSAASDGDDDDDDGERDEHT 120

Query: 1179 XXXXXXXXXXXXXXXXAPSLHTLLEGIPDPAAAHRTXXXXXXXXXXXXXXXXSLRTRKLK 1000
                             PSL TLLE +P+  A   T                S RTR+LK
Sbjct: 121  VLSAAAVAAAARNALRPPSLQTLLEDMPESVAIPHTYSSGGDSEGKSKSADKSSRTRRLK 180

Query: 999  HDVIEAVNELIQDIDTCHELIAEQAVEHIHHNEVILTLGRSRTVMEFLCAAKEKKRSFRV 820
            HDVIEAVNELIQDI+TCHE IAEQAVEHIH NEVILTLG S+TV+EFL AAKEKKRSFRV
Sbjct: 181  HDVIEAVNELIQDINTCHEQIAEQAVEHIHQNEVILTLGSSKTVLEFLYAAKEKKRSFRV 240

Query: 819  FVAEGAPRYQGHILAKELVARGLQTTVITDSAVFAMISRVNMVIVGAHAIMANGGVIAPV 640
            FVAEGAPRYQGH+LAKELV RGL TT+ITDSAVFAMISRVNMVIVGAHA+MANGGVI PV
Sbjct: 241  FVAEGAPRYQGHLLAKELVTRGLHTTLITDSAVFAMISRVNMVIVGAHAVMANGGVIVPV 300

Query: 639  GLNMVALAAQRHAVPFVVVAGSHKLCPLYPHNPEVLLNDLRSPSELLDFGEFSDCMDFGS 460
            GLNMVALAAQ+HAVPFVV+AGSHKLCPLYPHNPEVLLN+LRSPSELLDFGEFSDC+DFGS
Sbjct: 301  GLNMVALAAQKHAVPFVVLAGSHKLCPLYPHNPEVLLNELRSPSELLDFGEFSDCLDFGS 360

Query: 459  GNGSPLLHVVNPAFDYVPPKLVTLFITDTGGHNPSYMYRLIADYYSADDFVVQRK 295
            G GSPLLHVVNPAFDYVPPKLV+LFITDTGGHNPSYMYRLIADYYSADD VV+R+
Sbjct: 361  GTGSPLLHVVNPAFDYVPPKLVSLFITDTGGHNPSYMYRLIADYYSADDLVVRRR 415