BLASTX nr result
ID: Coptis25_contig00002190
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00002190
(952 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin... 163 5e-38
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi... 142 2e-31
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin... 138 2e-30
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro... 134 2e-29
gb|ADP76529.1| coclaurine N-methyltransferase [Aristolochia fimb... 123 6e-26
>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT gi|226897702|gb|ACO90222.1|
tetrahydroprotoberberine N-methyltransferase
[Eschscholzia californica]
Length = 350
Score = 163 bits (413), Expect = 5e-38
Identities = 75/107 (70%), Positives = 91/107 (85%)
Frame = +1
Query: 349 EDWYSNYIFPKGSVTVPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAA 528
+DWYS +IFPKG VT+ +STLLYFQDDV+ +D WV NG HMAR+VE W KLD+ I+AA
Sbjct: 241 DDWYSGFIFPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDETIEAA 300
Query: 529 RKILEPCLGSKDAVDKVITHSRTFCIGAYEQFSFNDGEEYMITHILF 669
R+ILEP LGSK+AV++VITH RTFCIG YEQFS+N+GEE+MIT ILF
Sbjct: 301 REILEPGLGSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQILF 347
Score = 103 bits (256), Expect = 8e-20
Identities = 43/72 (59%), Positives = 61/72 (84%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMK 182
+K CHVTG+TNS+AQ + IE++ + LEL+NVDVI ADVT +DT +T+DRI+V+E +EHMK
Sbjct: 143 YKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTKFDTDKTYDRILVVETIEHMK 202
Query: 183 NVGLFLRKISKW 218
N+ LF++K+S W
Sbjct: 203 NIQLFMKKLSTW 214
>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
tetrahydroprotoberberine N-methyltransferase [Papaver
bracteatum]
Length = 358
Score = 142 bits (357), Expect = 2e-31
Identities = 65/108 (60%), Positives = 85/108 (78%), Gaps = 1/108 (0%)
Frame = +1
Query: 349 EDWYSNYIFPKGSVTVPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAA 528
+DWYS +IFP G T+ A++LLYFQDDV+VVD WV NG HMAR+V+ W LDKN++AA
Sbjct: 248 DDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAA 307
Query: 529 RKILEPCL-GSKDAVDKVITHSRTFCIGAYEQFSFNDGEEYMITHILF 669
++IL P L GS +AV+ V+TH RTFC+G YEQFS NDG+E+M+ +LF
Sbjct: 308 KEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLF 355
Score = 92.8 bits (229), Expect = 1e-16
Identities = 39/72 (54%), Positives = 60/72 (83%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMK 182
+K CHVTG+TNS+AQ + + ++ + L L+NVD ILADVT Y++ +T+DR+++IEA+EHMK
Sbjct: 150 YKKCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQYESDKTYDRLLMIEAIEHMK 209
Query: 183 NVGLFLRKISKW 218
N+ LF++K+S W
Sbjct: 210 NLQLFMKKLSTW 221
>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT gi|67764091|gb|AAY79177.1|
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-
cis-N-methyltransferase [Papaver somniferum]
Length = 358
Score = 138 bits (348), Expect = 2e-30
Identities = 63/108 (58%), Positives = 84/108 (77%), Gaps = 1/108 (0%)
Frame = +1
Query: 349 EDWYSNYIFPKGSVTVPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAA 528
+DWYS +IFP G T+ A++LLYFQDDV+VVD WV NG HMAR+V+ W LDKN++AA
Sbjct: 248 DDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKNMEAA 307
Query: 529 RKILEPCL-GSKDAVDKVITHSRTFCIGAYEQFSFNDGEEYMITHILF 669
++IL P L GS + V+ V+TH RTFC+G YEQFS N+G+E+M+ +LF
Sbjct: 308 KEILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNGDEWMVAQLLF 355
Score = 93.6 bits (231), Expect = 6e-17
Identities = 39/72 (54%), Positives = 60/72 (83%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMK 182
+K CHVTG+TNS+AQ + + ++ + L L+NVD ILADVT Y++ +T+DR+++IEA+EHMK
Sbjct: 150 YKNCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAILADVTQYESDKTYDRLLMIEAIEHMK 209
Query: 183 NVGLFLRKISKW 218
N+ LF++K+S W
Sbjct: 210 NLQLFMKKLSTW 221
>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2
gi|226897730|gb|ACO90236.1| putative N-methyltransferase
[Papaver bracteatum]
Length = 358
Score = 134 bits (338), Expect = 2e-29
Identities = 64/108 (59%), Positives = 79/108 (73%), Gaps = 1/108 (0%)
Frame = +1
Query: 349 EDWYSNYIFPKGSVTVPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAA 528
+DWYS YIFP G T A +LLYFQDDV+VVD WV NG H ARTV+ W KLDKN++A
Sbjct: 248 DDWYSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKLDKNMEAV 307
Query: 529 RKILEPCL-GSKDAVDKVITHSRTFCIGAYEQFSFNDGEEYMITHILF 669
++IL P L G+ +AV+ VITH RT C+G Y QFS NDG+E+M +LF
Sbjct: 308 KEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDGDEWMNAQLLF 355
Score = 91.3 bits (225), Expect = 3e-16
Identities = 41/72 (56%), Positives = 57/72 (79%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMK 182
+K CHVTG+TNS+ Q + I ++ + L L NVDVILADVT Y++ +T+DRI+VI VEHMK
Sbjct: 150 YKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVILADVTQYESDKTYDRILVIGVVEHMK 209
Query: 183 NVGLFLRKISKW 218
N+ LF++K+S W
Sbjct: 210 NMQLFIKKLSTW 221
>gb|ADP76529.1| coclaurine N-methyltransferase [Aristolochia fimbriata]
Length = 357
Score = 123 bits (309), Expect = 6e-26
Identities = 54/107 (50%), Positives = 74/107 (69%)
Frame = +1
Query: 349 EDWYSNYIFPKGSVTVPGASTLLYFQDDVAVVDQWVENGKHMARTVEEWLHKLDKNIDAA 528
+DW++ Y FP GS +P AS LLYFQDDV++VD W+ +GKH RT EEW+ +LD N++
Sbjct: 248 DDWFAEYFFPPGSFAMPSASFLLYFQDDVSIVDHWILSGKHFHRTAEEWVKQLDTNLEKG 307
Query: 529 RKILEPCLGSKDAVDKVITHSRTFCIGAYEQFSFNDGEEYMITHILF 669
++ILE GSK+A K H R CI + E F +N GEE+M +H+LF
Sbjct: 308 KEILESKYGSKEAALKAFNHWRGLCIFSSEIFGYNGGEEWMTSHLLF 354
Score = 87.4 bits (215), Expect = 4e-15
Identities = 41/72 (56%), Positives = 53/72 (73%)
Frame = +3
Query: 3 FKGCHVTGITNSRAQKSIIEERCKNLELSNVDVILADVTNYDTKETFDRIIVIEAVEHMK 182
+K HVT +TNS QK IE++C+ LELSNV+V+L D+T + TFDRIIVI +EHMK
Sbjct: 149 YKKSHVTAVTNSATQKKYIEDQCQILELSNVEVLLEDITQLTMEATFDRIIVIGLLEHMK 208
Query: 183 NVGLFLRKISKW 218
N GL L+ IS+W
Sbjct: 209 NYGLLLQNISQW 220