BLASTX nr result
ID: Coptis25_contig00002103
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00002103
(1929 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002285593.1| PREDICTED: aspartic proteinase nepenthesin-2... 488 e-135
ref|XP_002515388.1| Aspartic proteinase nepenthesin-2 precursor,... 470 e-130
ref|XP_002324349.1| predicted protein [Populus trichocarpa] gi|2... 469 e-130
ref|XP_002283470.1| PREDICTED: aspartic proteinase nepenthesin-2... 460 e-127
ref|XP_003532146.1| PREDICTED: aspartic proteinase nepenthesin-1... 458 e-126
>ref|XP_002285593.1| PREDICTED: aspartic proteinase nepenthesin-2 [Vitis vinifera]
Length = 481
Score = 488 bits (1256), Expect = e-135
Identities = 247/453 (54%), Positives = 319/453 (70%), Gaps = 8/453 (1%)
Frame = -2
Query: 1733 GRPETLANHHHHHIIEVKSLFRATTCSEFPTRGNKGSMLPMFDKDGPCSPLSTGDMTEKA 1554
GR L+ H + + SL ++ CS P +K + L + K GPCS LS +
Sbjct: 30 GRKTALSTPSTLHNVHITSLMPSSVCSPSPKGDDKRASLEVIHKHGPCSKLSQDKGRSPS 89
Query: 1553 RNYTHVLIQDQARVQSIHSRISK---EGISVEPEAVRLPANSGKVFGTGNYYVTVGIGTP 1383
R T +L QD++RV SI SR++K +G ++ V LP+ SG GTGNY VTVG+GTP
Sbjct: 90 R--TQMLDQDESRVNSIRSRLAKNPADGGKLKGSKVTLPSKSGSTIGTGNYVVTVGLGTP 147
Query: 1382 KRDLPLVFDTGSDLTWIQCQPCLGGCYPQTDPKFEPLNSSSYSNITCNSTECIQLESGTG 1203
KRDL +FDTGSDLTW QC+PC CY Q +P F P S+SY+NI+C+S C +L+SGTG
Sbjct: 148 KRDLTFIFDTGSDLTWTQCEPCARYCYHQQEPIFNPSKSTSYTNISCSSPTCDELKSGTG 207
Query: 1202 NAPRCRASTCVYSITYGDNSYSQGFFAHETLAISPSDVFPNFQFGCGQSNGGLFKGTAGL 1023
N+P C ASTCVY I YGD SYS GFFA + LA++ +DVF NF FGCGQ+N GLF G AGL
Sbjct: 208 NSPSCSASTCVYGIQYGDQSYSVGFFAQDKLALTSTDVFNNFLFGCGQNNRGLFVGVAGL 267
Query: 1022 LGLGQDKISFVSQTSQKYKKLFSYCI--SPSSSGFLAFGD---SSSVTQFTPLLKDKRGP 858
+GLG++ +S VSQT+QKY KLFSYC+ + SS+G+L FG +S +FTP L + +GP
Sbjct: 268 IGLGRNALSLVSQTAQKYGKLFSYCLPSTSSSTGYLTFGSGGGTSKAVKFTPSLVNSQGP 327
Query: 857 SFYFLNLEGISVGGQELAISPSTFSLSGTIIDSGTVVTRLAPSAYAALRDAFQQAMKSYP 678
SFYFLNL ISVGG++L+ S S FS +GTIIDSGTV++RL P+AY+ LR +FQQ M YP
Sbjct: 328 SFYFLNLIAISVGGRKLSTSASVFSTAGTIIDSGTVISRLPPTAYSDLRASFQQQMSKYP 387
Query: 677 RASEFSLLDTCYDLSGNDTVTVPTIVLHFQGGTDLDVNQYGILLGPRQSQLCLAFIGNSN 498
+A+ S+LDTCYD S DTV VP I L+F G ++D++ GI SQ+CLAF GNS+
Sbjct: 388 KAAPASILDTCYDFSQYDTVDVPKINLYFSDGAEMDLDPSGIFYILNISQVCLAFAGNSD 447
Query: 497 ARDVAIIGNLQQKTVEVVYDVAGGKLGFGPGGC 399
A D+AI+GN+QQKT +VVYDVAGG++GF PGGC
Sbjct: 448 ATDIAILGNVQQKTFDVVYDVAGGRIGFAPGGC 480
>ref|XP_002515388.1| Aspartic proteinase nepenthesin-2 precursor, putative [Ricinus
communis] gi|223545332|gb|EEF46837.1| Aspartic proteinase
nepenthesin-2 precursor, putative [Ricinus communis]
Length = 494
Score = 470 bits (1210), Expect = e-130
Identities = 242/448 (54%), Positives = 314/448 (70%), Gaps = 9/448 (2%)
Frame = -2
Query: 1712 NHHHHHIIEVKSLFRATTC---SEFPTRGNKGSMLPMFDKDGPCSPLSTGDMTEKARNYT 1542
+ H H I + SL A +C ++ P+ NK + L + K GPCS L G E
Sbjct: 52 SQHTHTTIHLTSLLPAASCKPSTQVPSIENK-AFLKVVHKHGPCSDLRQGHKAEAQ---- 106
Query: 1541 HVLIQDQARVQSIHSRISKE-GIS--VEPEAVRLPANSGKVFGTGNYYVTVGIGTPKRDL 1371
++L+QDQ+RV SIHS++SK+ G+S A LPA G + G+GNY+VTVG+GTPK+D
Sbjct: 107 YILLQDQSRVDSIHSKLSKDSGLSDVKATAATTLPAKDGSIIGSGNYFVTVGLGTPKKDF 166
Query: 1370 PLVFDTGSDLTWIQCQPCLGGCYPQTDPKFEPLNSSSYSNITCNSTECIQLESGTGNAPR 1191
L+FDTGSDLTW QC+PC+ CY Q + F P S+SY+NI+C ST C L S TGN
Sbjct: 167 SLIFDTGSDLTWTQCEPCVKSCYNQKEAIFNPSQSTSYANISCGSTLCDSLASATGNIFN 226
Query: 1190 CRASTCVYSITYGDNSYSQGFFAHETLAISPSDVFPNFQFGCGQSNGGLFKGTAGLLGLG 1011
C +STCVY I YGD+S+S GFF E L+++ +DVF +F FGCGQ+N GLF G AGLLGLG
Sbjct: 227 CASSTCVYGIQYGDSSFSIGFFGKEKLSLTATDVFNDFYFGCGQNNKGLFGGAAGLLGLG 286
Query: 1010 QDKISFVSQTSQKYKKLFSYCI--SPSSSGFLAFGDSSSVT-QFTPLLKDKRGPSFYFLN 840
+DK+S VSQT+Q+Y K+FSYC+ S SS+GFL FG S+S + FTPL G SFY L+
Sbjct: 287 RDKLSLVSQTAQRYNKIFSYCLPSSSSSTGFLTFGGSTSKSASFTPLATISGGSSFYGLD 346
Query: 839 LEGISVGGQELAISPSTFSLSGTIIDSGTVVTRLAPSAYAALRDAFQQAMKSYPRASEFS 660
L GISVGG++LAISPS FS +GTIIDSGTV+TRL P+AY+AL F++ M YP A S
Sbjct: 347 LTGISVGGRKLAISPSVFSTAGTIIDSGTVITRLPPAAYSALSSTFRKLMSQYPAAPALS 406
Query: 659 LLDTCYDLSGNDTVTVPTIVLHFQGGTDLDVNQYGILLGPRQSQLCLAFIGNSNARDVAI 480
+LDTC+D S +DT++VP I L F GG +D+++ GI +Q+CLAF GNS+A DVAI
Sbjct: 407 ILDTCFDFSNHDTISVPKIGLFFSGGVVVDIDKTGIFYVNDLTQVCLAFAGNSDASDVAI 466
Query: 479 IGNLQQKTVEVVYDVAGGKLGFGPGGCN 396
GN+QQKT+EVVYD A G++GF P GC+
Sbjct: 467 FGNVQQKTLEVVYDGAAGRVGFAPAGCS 494
>ref|XP_002324349.1| predicted protein [Populus trichocarpa] gi|222865783|gb|EEF02914.1|
predicted protein [Populus trichocarpa]
Length = 490
Score = 469 bits (1208), Expect = e-130
Identities = 241/453 (53%), Positives = 305/453 (67%), Gaps = 12/453 (2%)
Frame = -2
Query: 1718 LANHHHHHIIEVKSLFRATTCSE----FPTRGNKGSMLPMFDKDGPCSPLSTGDMTEKAR 1551
+A HH H IEV SL + +C NK S L + K GPCS LS D A
Sbjct: 40 VAESHHSHSIEVSSLLPSASCKPSTKVLSNNDNKAS-LKVVHKHGPCSKLSQ-DEASAAP 97
Query: 1550 NYTHVLIQDQARVQSIHSRISKEGIS-----VEPEAVRLPANSGKVFGTGNYYVTVGIGT 1386
+T +L+QDQ+RV+SIHSR+S S ++ +PA G G+GNY VTVG+GT
Sbjct: 98 THTEILLQDQSRVKSIHSRLSNSKTSGGKDVKVTDSTTIPAKDGSTVGSGNYIVTVGLGT 157
Query: 1385 PKRDLPLVFDTGSDLTWIQCQPCLGGCYPQTDPKFEPLNSSSYSNITCNSTECIQLESGT 1206
PK+DL L+FDTGSD+TW QCQPC CY Q + F+P S+SY+NI+C+S+ C L S T
Sbjct: 158 PKKDLSLIFDTGSDITWTQCQPCARSCYKQKEQIFDPSQSTSYTNISCSSSICNSLTSAT 217
Query: 1205 GNAPRCRASTCVYSITYGDNSYSQGFFAHETLAISPSDVFPNFQFGCGQSNGGLFKGTAG 1026
GN P C +S CVY I YGD+S+S GFF E L ++ +D F N FGCGQ+N GLF G+AG
Sbjct: 218 GNTPGCASSACVYGIQYGDSSFSVGFFGTEKLTLTSTDAFNNIYFGCGQNNQGLFGGSAG 277
Query: 1025 LLGLGQDKISFVSQTSQKYKKLFSYCI--SPSSSGFLAFGDSSSV-TQFTPLLKDKRGPS 855
LLGLG+DK+S VSQT+QKY K+FSYC+ S SS+GFL FG S+S +FTPL GPS
Sbjct: 278 LLGLGRDKLSVVSQTAQKYNKIFSYCLPSSSSSTGFLTFGGSASKNAKFTPLSTISAGPS 337
Query: 854 FYFLNLEGISVGGQELAISPSTFSLSGTIIDSGTVVTRLAPSAYAALRDAFQQAMKSYPR 675
FY L+ GISVGG++LAIS S FS +G IIDSGTV+TRL P+AY+ALR +F+ M YP
Sbjct: 338 FYGLDFTGISVGGKKLAISASVFSTAGAIIDSGTVITRLPPAAYSALRASFRNLMSKYPM 397
Query: 674 ASEFSLLDTCYDLSGNDTVTVPTIVLHFQGGTDLDVNQYGILLGPRQSQLCLAFIGNSNA 495
S+LDTCYD S T++VP I F G ++D++ GIL SQ+CLAF GNS+A
Sbjct: 398 TKALSILDTCYDFSSYTTISVPKIGFSFSSGIEVDIDATGILYASSLSQVCLAFAGNSDA 457
Query: 494 RDVAIIGNLQQKTVEVVYDVAGGKLGFGPGGCN 396
DV I GN+QQKT+EV YD + GK+GF PGGC+
Sbjct: 458 TDVFIFGNVQQKTLEVFYDGSAGKVGFAPGGCS 490
>ref|XP_002283470.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Vitis vinifera]
Length = 490
Score = 460 bits (1183), Expect = e-127
Identities = 231/452 (51%), Positives = 310/452 (68%), Gaps = 12/452 (2%)
Frame = -2
Query: 1715 ANHHH----HHIIEVKSLFRATTCSEFPTRGNKGSMLPMFDKDGPCSPLSTGDMTEKARN 1548
A HH HH + + SL ++ CS P ++ + L + K GPCS L +
Sbjct: 41 AESHHVQPIHHNVHITSLMPSSACSPSPKGHDQRASLEVVHKHGPCSKLRPHKANSPS-- 98
Query: 1547 YTHVLIQDQARVQSIHSRISKE---GISVEPEAVRLPANSGKVFGTGNYYVTVGIGTPKR 1377
+T +L QD++RV SI SR++K G +++ LP+ S G+GNY VTVG+G+PKR
Sbjct: 99 HTQILAQDESRVASIQSRLAKNLAGGSNLKASKATLPSKSASTLGSGNYVVTVGLGSPKR 158
Query: 1376 DLPLVFDTGSDLTWIQCQPCLGGCYPQTDPKFEPLNSSSYSNITCNSTECIQLESGTGNA 1197
DL +FDTGSDLTW QC+PC+G CY Q + F+P S SYSN++C+S C +LES TGN+
Sbjct: 159 DLTFIFDTGSDLTWTQCEPCVGYCYQQREHIFDPSTSLSYSNVSCDSPSCEKLESATGNS 218
Query: 1196 PRCRASTCVYSITYGDNSYSQGFFAHETLAISPSDVFPNFQFGCGQSNGGLFKGTAGLLG 1017
P C +STC+Y I YGD SYS GFFA E L+++ +DVF NFQFGCGQ+N GLF GTAGLLG
Sbjct: 219 PGCSSSTCLYGIRYGDGSYSIGFFAREKLSLTSTDVFNNFQFGCGQNNRGLFGGTAGLLG 278
Query: 1016 LGQDKISFVSQTSQKYKKLFSYCI--SPSSSGFLAFGD---SSSVTQFTPLLKDKRGPSF 852
L ++ +S VSQT+QKY K+FSYC+ S SS+G+L+FG S +FTP + PSF
Sbjct: 279 LARNPLSLVSQTAQKYGKVFSYCLPSSSSSTGYLSFGSGDGDSKAVKFTPSEVNSDYPSF 338
Query: 851 YFLNLEGISVGGQELAISPSTFSLSGTIIDSGTVVTRLAPSAYAALRDAFQQAMKSYPRA 672
YFL++ GISVG ++L I S FS +GTIIDSGTV++RL P+ Y++++ F++ M YPR
Sbjct: 339 YFLDMVGISVGERKLPIPKSVFSTAGTIIDSGTVISRLPPTVYSSVQKVFRELMSDYPRV 398
Query: 671 SEFSLLDTCYDLSGNDTVTVPTIVLHFQGGTDLDVNQYGILLGPRQSQLCLAFIGNSNAR 492
S+LDTCYDLS TV VP I+L+F GG ++D+ GI+ + SQ+CLAF GNS+
Sbjct: 399 KGVSILDTCYDLSKYKTVKVPKIILYFSGGAEMDLAPEGIIYVLKVSQVCLAFAGNSDDD 458
Query: 491 DVAIIGNLQQKTVEVVYDVAGGKLGFGPGGCN 396
+VAIIGN+QQKT+ VVYD A G++GF P GCN
Sbjct: 459 EVAIIGNVQQKTIHVVYDDAEGRVGFAPSGCN 490
>ref|XP_003532146.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Glycine max]
Length = 488
Score = 458 bits (1179), Expect = e-126
Identities = 230/447 (51%), Positives = 305/447 (68%), Gaps = 10/447 (2%)
Frame = -2
Query: 1709 HHHHHIIEVKSLFRATTCSEFPTRGNKGSMLPMFDKDGPCSPLSTGDMTEKARN-YTHVL 1533
H + H++ + SL +++CS + + L + K GPCS L+ D K++ ++ +L
Sbjct: 41 HQYTHLVHLSSLLPSSSCSSSAKGPKRKASLEVVHKHGPCSQLNNHDGKAKSKTPHSEIL 100
Query: 1532 IQDQARVQSIHSRISK----EGISVEPEAVRLPANSGKVFGTGNYYVTVGIGTPKRDLPL 1365
QD+ RV+ I+SRISK + E ++V LPA SG + G+GNY+V VG+GTPKRDL L
Sbjct: 101 NQDKERVKYINSRISKNLGQDSSVSELDSVTLPAKSGSLIGSGNYFVVVGLGTPKRDLSL 160
Query: 1364 VFDTGSDLTWIQCQPCLGGCYPQTDPKFEPLNSSSYSNITCNSTECIQLESGTGNAPRCR 1185
+FDTGSDLTW QC+PC CY Q D F+P S+SYSNITC ST C QL + TGN P C
Sbjct: 161 IFDTGSDLTWTQCEPCARSCYKQQDAIFDPSKSTSYSNITCTSTLCTQLSTATGNEPGCS 220
Query: 1184 AST--CVYSITYGDNSYSQGFFAHETLAISPSDVFPNFQFGCGQSNGGLFKGTAGLLGLG 1011
AST C+Y I YGD+S+S G+F+ E L+++ +D+ NF FGCGQ+N GLF G+AGL+GLG
Sbjct: 221 ASTKACIYGIQYGDSSFSVGYFSRERLSVTATDIVDNFLFGCGQNNQGLFGGSAGLIGLG 280
Query: 1010 QDKISFVSQTSQKYKKLFSYCI--SPSSSGFLAFGDSS-SVTQFTPLLKDKRGPSFYFLN 840
+ ISFV QT+ Y+K+FSYC+ + SS+G L+FG ++ S ++TP RG SFY L+
Sbjct: 281 RHPISFVQQTAAVYRKIFSYCLPATSSSTGRLSFGTTTTSYVKYTPFSTISRGSSFYGLD 340
Query: 839 LEGISVGGQELAISPSTFSLSGTIIDSGTVVTRLAPSAYAALRDAFQQAMKSYPRASEFS 660
+ GISVGG +L +S STFS G IIDSGTV+TRL P+AY ALR AF+Q M YP A E S
Sbjct: 341 ITGISVGGAKLPVSSSTFSTGGAIIDSGTVITRLPPTAYTALRSAFRQGMSKYPSAGELS 400
Query: 659 LLDTCYDLSGNDTVTVPTIVLHFQGGTDLDVNQYGILLGPRQSQLCLAFIGNSNARDVAI 480
+LDTCYDLSG + ++P I F GG + + GIL Q+CLAF N + DV I
Sbjct: 401 ILDTCYDLSGYEVFSIPKIDFSFAGGVTVQLPPQGILYVASAKQVCLAFAANGDDSDVTI 460
Query: 479 IGNLQQKTVEVVYDVAGGKLGFGPGGC 399
GN+QQKT+EVVYDV GG++GFG GGC
Sbjct: 461 YGNVQQKTIEVVYDVGGGRIGFGAGGC 487