BLASTX nr result
ID: Coptis25_contig00001772
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00001772
(1497 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002529521.1| cysteine-type endopeptidase, putative [Ricin... 262 2e-67
ref|XP_003546773.1| PREDICTED: ubiquitin carboxyl-terminal hydro... 254 4e-65
ref|XP_003531525.1| PREDICTED: ubiquitin carboxyl-terminal hydro... 252 2e-64
ref|XP_002885042.1| ubiquitin-specific protease 25 [Arabidopsis ... 223 1e-55
ref|NP_566486.1| ubiquitin carboxyl-terminal hydrolase 25 [Arabi... 219 2e-54
>ref|XP_002529521.1| cysteine-type endopeptidase, putative [Ricinus communis]
gi|223531005|gb|EEF32859.1| cysteine-type endopeptidase,
putative [Ricinus communis]
Length = 644
Score = 262 bits (670), Expect = 2e-67
Identities = 184/477 (38%), Positives = 243/477 (50%), Gaps = 6/477 (1%)
Frame = +2
Query: 2 VLVIQLKRFEGIHGGKIDRAIAFEEGLLLSNYMCKESQDPCPQYNLYGTIVHSGYSPDSG 181
+LVIQLKRFEGI GGKID+AIAFEE L+LS++MCK SQDP P+YNL+GTIVHSGYSP+SG
Sbjct: 247 ILVIQLKRFEGIFGGKIDKAIAFEEVLVLSSFMCKGSQDPRPEYNLFGTIVHSGYSPESG 306
Query: 182 HYYAYIKDAMGRWYCCNDSFVSISNLQEVVSEKVYILFFARSNQRPRPTKSPSVTTGVEP 361
HYYAYIKDA GRWYCCNDS+V++S +Q+V+SEKVYILFF+RSNQRP + PS T
Sbjct: 307 HYYAYIKDATGRWYCCNDSYVTLSTMQDVLSEKVYILFFSRSNQRP-GSAGPSFTP---- 361
Query: 362 PHCNGHDASSSVTNGMKPPGCNGHDASSSVTNGVKPPHCNGHDASSSVANGAKPQYYNGH 541
+KP NG +AS+ V P N DA +SV ++
Sbjct: 362 ---------------IKPCNPNGCEASNISKVTVSP---NAVDAEASVEQPSRKDI---- 399
Query: 542 AASYSDPNEVKPLHSNGHNAPQSIRGAVPPKPVFMGSYGPDNSKKLIPTISKNDKVPSSP 721
P S P S R V +G SK+++PT + S
Sbjct: 400 -----------PSMSKVDKVPSSPR-------VKFNIFGNSLSKRILPTGDGKAESHKSQ 441
Query: 722 RIKFNILKSSDSKELRPXXXXXXXXYAGYHIDIKERKEMKKITKEMPSVTNVAHVNESLD 901
++ N +K+R ++K K+MPS N N+S
Sbjct: 442 NMEMN-------------------------GSMKDRVYVEKHDKDMPSTINGNGCNKSRK 476
Query: 902 ALDPEPMPHANGKIAGSSVRPMKTEGSEGGGFALI------VAQDRRPDCGVVLQNACEK 1063
D E I +S + G+ G + + D+ P LQN
Sbjct: 477 INDGE----TGQAITKASANGVTQNGASDSGKPCLYESNGSASTDKGPG-QHKLQNGGVN 531
Query: 1064 NLSFVSGSKRKLLDDECSKSVSPDVHQAAVEKIDEISSSRLAKDASSRLRSCGWYDEVHR 1243
S + KRKL +D C + V+++ E L ++AS+ LRSCGW +V+
Sbjct: 532 CHSGIPELKRKLKEDSCILIAQDAQSRTEVKELKE----ALKQEASTVLRSCGWSSKVYS 587
Query: 1244 FMRGKKRLCAEKGGDAISDTELKKLLIEEASQIFKRQIPASLTQHLINYLKSFHQGK 1414
FM +KRLCA + G+ SD +LKKLLI EA F QIP SL + L+ L+SF Q K
Sbjct: 588 FMCSRKRLCAREPGNLPSDIDLKKLLIAEAKSTFISQIPESLKEDLVKRLQSFSQEK 644
>ref|XP_003546773.1| PREDICTED: ubiquitin carboxyl-terminal hydrolase 25-like [Glycine
max]
Length = 652
Score = 254 bits (649), Expect = 4e-65
Identities = 175/472 (37%), Positives = 245/472 (51%)
Frame = +2
Query: 2 VLVIQLKRFEGIHGGKIDRAIAFEEGLLLSNYMCKESQDPCPQYNLYGTIVHSGYSPDSG 181
+LVIQLKRFEGI GGKID+A+AFEE L+LS++MCK SQDP P+Y L+GTIVHSGYSP+SG
Sbjct: 236 ILVIQLKRFEGILGGKIDKAVAFEEVLVLSSFMCKASQDPQPEYKLFGTIVHSGYSPESG 295
Query: 182 HYYAYIKDAMGRWYCCNDSFVSISNLQEVVSEKVYILFFARSNQRPRPTKSPSVTTGVEP 361
HYYAYIKDAMGRWYCC+DS V+++ LQEV+SEKVYILFF+R+NQRP + + + GV+
Sbjct: 296 HYYAYIKDAMGRWYCCDDSCVTVATLQEVLSEKVYILFFSRTNQRPVASCNSLASNGVK- 354
Query: 362 PHCNGHDASSSVTNGMKPPGCNGHDASSSVTNGVKPPHCNGHDASSSVANGAKPQYYNGH 541
PH NG S G+ P H S+S + K A + ++ K
Sbjct: 355 PHSNGSQTSECPKVGVPPKAV--HVKSNSELSPCKDMPSVSKTAKAPSSSRVKFDINGSS 412
Query: 542 AASYSDPNEVKPLHSNGHNAPQSIRGAVPPKPVFMGSYGPDNSKKLIPTISKNDKVPSSP 721
+ P V N P I G V S +N K K+ +P+S
Sbjct: 413 SKRNPAPVSVNGKVDVSRNQPSVINGHV------KDSVSLENGK-------KDSSLPTSN 459
Query: 722 RIKFNILKSSDSKELRPXXXXXXXXYAGYHIDIKERKEMKKITKEMPSVTNVAHVNESLD 901
I K + + K+ ++ +VTN N+++D
Sbjct: 460 GID------------------------------KNKVAVDKLKRKESTVTNGHTGNQTVD 489
Query: 902 ALDPEPMPHANGKIAGSSVRPMKTEGSEGGGFALIVAQDRRPDCGVVLQNACEKNLSFVS 1081
+ +K++ E + ++A R PD ++ N +
Sbjct: 490 ------------------IHSVKSDLKEDTDRSRVIA-GRVPD-NFKHESNDLNNKPTIL 529
Query: 1082 GSKRKLLDDECSKSVSPDVHQAAVEKIDEISSSRLAKDASSRLRSCGWYDEVHRFMRGKK 1261
G+KRKL +D C QA V+++ +I L K+A S LRSCGW D+V+ FMR KK
Sbjct: 530 GNKRKLQEDPCILLAHDGQSQARVQELKDI----LGKEAKSVLRSCGWTDKVYEFMRSKK 585
Query: 1262 RLCAEKGGDAISDTELKKLLIEEASQIFKRQIPASLTQHLINYLKSFHQGKQ 1417
L A++ G+ + E++KLLI +A F +IP SL Q +I L+SF Q K+
Sbjct: 586 -LHAQEAGNLTNGDEIRKLLIGDAQGAFISKIPESLKQDMIERLRSFSQEKK 636
>ref|XP_003531525.1| PREDICTED: ubiquitin carboxyl-terminal hydrolase 25-like [Glycine
max]
Length = 641
Score = 252 bits (644), Expect = 2e-64
Identities = 179/473 (37%), Positives = 241/473 (50%), Gaps = 2/473 (0%)
Frame = +2
Query: 2 VLVIQLKRFEGIHGGKIDRAIAFEEGLLLSNYMCKESQDPCPQYNLYGTIVHSGYSPDSG 181
+LVIQLKRFEGI GGKID+A+AFEE L+LS++MCK SQDP P+Y L+GTIVHSGYSP+SG
Sbjct: 235 ILVIQLKRFEGILGGKIDKAVAFEEVLVLSSFMCKASQDPQPEYKLFGTIVHSGYSPESG 294
Query: 182 HYYAYIKDAMGRWYCCNDSFVSISNLQEVVSEKVYILFFARSNQRPRPTKSPSVTTGVEP 361
HYYAYIKDAMGRWYCC+DS V+++ LQEV+SEKVYILFF+R+NQRP + + + GV+
Sbjct: 295 HYYAYIKDAMGRWYCCDDSCVTVATLQEVLSEKVYILFFSRTNQRPVSSSNSLASNGVK- 353
Query: 362 PHCNGHDASSSVTNGMKPPGCNGHDASSSVTNGVKPPHCNGHDASSSVANGAKPQYYNGH 541
PH NG S G+ P K H N
Sbjct: 354 PHSNGSQTSECPKVGVPP----------------KAVHAKS----------------NSE 381
Query: 542 AASYSDPNEVKPLHSNGHNAPQSIRGAVPPKPVFMGSYGPDNSKKLIPTISKNDKVPSSP 721
+S+ D P S AP S R V G +SK+ +S N KV
Sbjct: 382 LSSWKD----IPRVSKTAKAPSSSR-------VKFDINGSSSSKRNSAPVSVNGKV---- 426
Query: 722 RIKFNILKSSDSKELRPXXXXXXXXYAGYHIDIKERKEMKKITKEMPSVTNVAHVNESLD 901
++ ++ S H+ E K +P+ + S+D
Sbjct: 427 ----DVCRNQPSA-------------VNGHVKDSASLENGKEDSSLPTRNGIDENKVSVD 469
Query: 902 ALDPEPMPHANGKIAGSSV--RPMKTEGSEGGGFALIVAQDRRPDCGVVLQNACEKNLSF 1075
L + +NG +V +K++ E + I+A R PD N N
Sbjct: 470 KLKRKESTVSNGHTGNQTVDIHSVKSDLKEDTDRSRIIA-GRVPDNFKQESNGL-NNKPT 527
Query: 1076 VSGSKRKLLDDECSKSVSPDVHQAAVEKIDEISSSRLAKDASSRLRSCGWYDEVHRFMRG 1255
+ G+KRKL D C QA V+++ +I L K+A S LRS GW D+V+ FMR
Sbjct: 528 ILGNKRKLQGDPCILLAHDGQSQARVQELKDI----LGKEAKSVLRSIGWTDKVYEFMRS 583
Query: 1256 KKRLCAEKGGDAISDTELKKLLIEEASQIFKRQIPASLTQHLINYLKSFHQGK 1414
KK L A++ G+ + E++KLLI +A F +IP SL Q +I L+SF Q K
Sbjct: 584 KK-LRAQEAGNLTNGDEIRKLLIGDAQGAFISKIPESLKQDMIQRLQSFSQEK 635
>ref|XP_002885042.1| ubiquitin-specific protease 25 [Arabidopsis lyrata subsp. lyrata]
gi|297330882|gb|EFH61301.1| ubiquitin-specific protease
25 [Arabidopsis lyrata subsp. lyrata]
Length = 662
Score = 223 bits (567), Expect = 1e-55
Identities = 180/490 (36%), Positives = 230/490 (46%), Gaps = 17/490 (3%)
Frame = +2
Query: 2 VLVIQLKRFEGIHGGKIDRAIAFEEGLLLSNYMCKESQDPCPQYNLYGTIVHSGYSPDSG 181
+LVIQLKRF GI GGKID+AI+F E L+LSN+M K S+DP P+Y L+G IVHSG+SP+SG
Sbjct: 234 ILVIQLKRFGGIFGGKIDKAISFGEILVLSNFMSKASKDPQPEYKLFGIIVHSGFSPESG 293
Query: 182 HYYAYIKDAMGRWYCCNDSFVSISNLQEVVSEKVYILFFARSNQRPRPTKSPSVTTGVEP 361
HYYAY+KD++GRWYCCNDSFVS+S LQEV+SEK YILFF+RSNQRP K+ + G
Sbjct: 294 HYYAYVKDSLGRWYCCNDSFVSLSTLQEVLSEKAYILFFSRSNQRPASAKTLVTSNGTTS 353
Query: 362 PHCNGHDASSSVTNGMKPPGCNGHDASSSVTNGVKPPHCNGHDASSSVANGAKPQYYNGH 541
NG + S N K G NGV KPQ
Sbjct: 354 HEVNGCETS----NPQKFIG---------PLNGV----------------NMKPQ----- 379
Query: 542 AASYSDPNEVKPLHSNGHNAPQSIRGAVPPKPVFMGSYGPDNSKKLIPTISKNDKVPSSP 721
S +VK S H + PK + DN SK +K P P
Sbjct: 380 --SEQSFQKVKLASSKPHK-------FIGPKLRAEQALQEDNLLS-----SKVEKAPLKP 425
Query: 722 RIKFNILKSSDSKELRPXXXXXXXXYAGYHIDIKERKE--MKKITKEMPSVTNVAHVNES 895
K I + +K + P + +I K KE + + + S T E+
Sbjct: 426 HAKVIISVNLGAKRVSPSVNGRLSFHQDENIAPKANKENSVSVLPTRVKSGTERKFGTEN 485
Query: 896 LDALDPEPMPHANGKIAGSS-----VRP-MKTEGSEGGGFALIVAQDRRPDCGV------ 1039
E NG GSS V P ++ GS GG +D CG
Sbjct: 486 GGNGVKE-----NGSAPGSSNHKVTVHPHERSNGSCNGGDHY---KDNLHPCGSNGSQNG 537
Query: 1040 VLQNACEKNLSFVSGSK---RKLLDDECSKSVSPDVHQAAVEKIDEISSSRLAKDASSRL 1210
+ E+N + SK +D C + + +E I E L KDA S L
Sbjct: 538 TVHQEIERNGVSTTQSKGLCSSTNEDPCILLRKDESSRNELEAIKE----SLKKDALSHL 593
Query: 1211 RSCGWYDEVHRFMRGKKRLCAEKGGDAISDTELKKLLIEEASQIFKRQIPASLTQHLINY 1390
RSCGWYD+VH MR KKRL E+ G +LK+LLIE+ K QIP L L+N
Sbjct: 594 RSCGWYDKVHISMRAKKRLRTEQSGGE-DGNDLKRLLIEDVKSSLKSQIPEGLKADLVNR 652
Query: 1391 LKSFHQGKQS 1420
+ + K S
Sbjct: 653 IWEISKKKYS 662
>ref|NP_566486.1| ubiquitin carboxyl-terminal hydrolase 25 [Arabidopsis thaliana]
gi|75262798|sp|Q9FPS2.1|UBP25_ARATH RecName:
Full=Ubiquitin carboxyl-terminal hydrolase 25; AltName:
Full=Deubiquitinating enzyme 25; Short=AtUBP25; AltName:
Full=Ubiquitin thioesterase 25; AltName:
Full=Ubiquitin-specific-processing protease 25
gi|11993490|gb|AAG42763.1|AF302673_1 ubiquitin-specific
protease 25 [Arabidopsis thaliana]
gi|60543331|gb|AAX22263.1| At3g14400 [Arabidopsis
thaliana] gi|332641992|gb|AEE75513.1| ubiquitin
carboxyl-terminal hydrolase 25 [Arabidopsis thaliana]
Length = 661
Score = 219 bits (557), Expect = 2e-54
Identities = 167/490 (34%), Positives = 227/490 (46%), Gaps = 17/490 (3%)
Frame = +2
Query: 2 VLVIQLKRFEGIHGGKIDRAIAFEEGLLLSNYMCKESQDPCPQYNLYGTIVHSGYSPDSG 181
+LVIQLKRF GI GGKID+AI+F E L+LSN+M K S+DP P+Y L+G IVHSG+SP+SG
Sbjct: 234 ILVIQLKRFGGIFGGKIDKAISFGEILVLSNFMSKASKDPQPEYKLFGIIVHSGFSPESG 293
Query: 182 HYYAYIKDAMGRWYCCNDSFVSISNLQEVVSEKVYILFFARSNQRPRPTKSPSVTTGVEP 361
HYYAY+KD++GRWYCCNDSFVS+S LQEV+SEK YILFF+RSNQRP K+ + G
Sbjct: 294 HYYAYVKDSLGRWYCCNDSFVSLSTLQEVLSEKAYILFFSRSNQRPASAKTLVTSNGTTS 353
Query: 362 PHCNGHDASSSVTNGMKPPGCNGHDASSSVTNGVKPPHCNGHDASSSVANGAKPQYYNGH 541
NG + S+ PQ + G
Sbjct: 354 HEVNGCETSN-------------------------------------------PQKFIGP 370
Query: 542 AASYSDPNEVKPLHSNGHNAPQSIRGAVPPKPVFMGSYGPDNSKKLIPTISKNDKVPSSP 721
++ + + G+ A + PKP + DN SK +K P P
Sbjct: 371 LNGFNMKPQAEQSFQKGNLASSKPHKFIRPKPRAEQAPLEDNLLS-----SKVEKAPLRP 425
Query: 722 RIKFNILKSSDSKELRPXXXXXXXXYAGYHID--IKERKEMKKITKEMPSVTNVAHVNES 895
K +I + +K + P +H D I + + +P+ N +
Sbjct: 426 HAKVSISVNLGAKRVSPVNGR-----LSFHQDENIAPKANKENSVSVLPTKVN-SGTERK 479
Query: 896 LDALDPEPMPHANGKIAGSSVRPM------KTEGSEGGGFALIVAQDRRPDCGV-VLQNA 1054
+ NG GSS + ++ GS GG +D CG QN
Sbjct: 480 FGTENGGNGVKENGSAPGSSNHKVALHPHERSNGSSNGGDH---HKDNLHPCGSNGSQNG 536
Query: 1055 C---EKNLSFVSGSKRKLL-----DDECSKSVSPDVHQAAVEKIDEISSSRLAKDASSRL 1210
E + VS ++ K L +D C + + +E I E L KDA S L
Sbjct: 537 TAHPETERNGVSTTQSKGLCSSTKEDPCILLRKDESSRNELEAIKE----SLKKDALSHL 592
Query: 1211 RSCGWYDEVHRFMRGKKRLCAEKGGDAISDTELKKLLIEEASQIFKRQIPASLTQHLINY 1390
RSCGWYD+V M KKRL E+ G ++LK+ LIE+ K QIP L L+N
Sbjct: 593 RSCGWYDKVLISMHAKKRLRTEQSGGE-DGSDLKRRLIEDVKSSLKSQIPEELKADLVNR 651
Query: 1391 LKSFHQGKQS 1420
+ + K S
Sbjct: 652 IWEISKKKYS 661