BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00001695
         (1364 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-meth...   659   0.0  
gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictr...   577   e-162
gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein ...   521   e-145
dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]        451   e-124
gb|AAQ01669.1| (R,S)-norcoclaurine 6-O-methyltransferase [Papave...   431   e-118

>sp|Q9LEL6.1|6OMT_COPJA RecName: Full=(RS)-norcoclaurine 6-O-methyltransferase; AltName:
            Full=S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase; Short=6-OMT
            gi|9711914|dbj|BAB08004.1|
            S-adenosyl-L-methionine:norcoclaurine
            6-O-methyltransferase [Coptis japonica]
          Length = 347

 Score =  659 bits (1700), Expect = 0.0
 Identities = 323/347 (93%), Positives = 328/347 (94%)
 Frame = -1

Query: 1259 MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPV 1080
            MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHN+GTSMTLSELSSRLPSQPV
Sbjct: 1    MEVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPV 60

Query: 1079 NEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMA 900
            NEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAK+LVKGWDKCMVGSILAITDKDFMA
Sbjct: 61   NEDALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMA 120

Query: 899  PWHYLKDGLAGESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 720
            PWHYLKDGL+GESGTAFEKALG NIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN
Sbjct: 121  PWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 180

Query: 719  IFNXXXXXXXXXXXXXXXVRNIANAFPHIKCIVYDLPHVIADSPGYSEVHCVAGDMFKFI 540
            IFN               VRNIANAFPHIKC VYDLPHVIADSPGYSEVHCVAGDMFKFI
Sbjct: 181  IFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFI 240

Query: 539  PKADAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTKMRLTL 360
            PKADAIMMKCILHDWDDKECIEILKRCKEAVP++GGKVIIVDIVLNVQSEHPYTKMRLTL
Sbjct: 241  PKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTL 300

Query: 359  DLDMMLNTGGKERTEEEWKNLIHDAGYKGHKITQITAVQSVIEVYPY 219
            DLDMMLNTGGKERTEEEWK LIHDAGYKGHKITQITAVQSVIE YPY
Sbjct: 301  DLDMMLNTGGKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYPY 347


>gb|AAU20765.1| (S)-norcoclaurine 6-O-methyltransferase [Thalictrum flavum subsp.
            glaucum]
          Length = 350

 Score =  577 bits (1488), Expect = e-162
 Identities = 278/347 (80%), Positives = 308/347 (88%), Gaps = 2/347 (0%)
 Frame = -1

Query: 1253 VKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPVNE 1074
            + K+NLSSQAKLWNFIYGFA+SLVLK AVQLDLANIIHN+G+ MTLSELS  LPSQPVN+
Sbjct: 4    INKENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQ 63

Query: 1073 DALYRVMRYLVHMKLFTKASIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMAPW 894
            DALYRV+RYLVHMKLFTK+SIDGELRYGLAPPAKFLVKGWDKCM+G+IL ITDKDFMAPW
Sbjct: 64   DALYRVLRYLVHMKLFTKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPW 123

Query: 893  HYLKDGLA--GESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 720
            HYLK+G+   G + TAFEKALG NIW YMAEHPEKNQLFNE MAND+RLIMSALVKEC +
Sbjct: 124  HYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRLIMSALVKECSS 183

Query: 719  IFNXXXXXXXXXXXXXXXVRNIANAFPHIKCIVYDLPHVIADSPGYSEVHCVAGDMFKFI 540
            +F+               VRNIA AFPHIKC VYDLPHVIADSPGY+E++ + GDMFK+I
Sbjct: 184  MFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKYI 243

Query: 539  PKADAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTKMRLTL 360
            P ADAIMMKCILHDWDDKECIEILKRCK+AVP +GGKVII+DI+L+V+SEHPYTKMRLTL
Sbjct: 244  PNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMRLTL 303

Query: 359  DLDMMLNTGGKERTEEEWKNLIHDAGYKGHKITQITAVQSVIEVYPY 219
            DLDMMLNTGGKERTEEEWK LIHDAGYKG+KIT I+AVQSVIE YPY
Sbjct: 304  DLDMMLNTGGKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYPY 350


>gb|ACO90250.1| norcoclaurine 6-O-methyltransferase-like protein [Thalictrum flavum]
          Length = 346

 Score =  521 bits (1341), Expect = e-145
 Identities = 256/345 (74%), Positives = 294/345 (85%), Gaps = 3/345 (0%)
 Frame = -1

Query: 1244 DNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPVNEDAL 1065
            ++LSSQAKLWN+IYGFA+SLVLKCAVQLDLANIIH++GT MTLSELSSRLPSQPVN+DAL
Sbjct: 2    EDLSSQAKLWNYIYGFADSLVLKCAVQLDLANIIHDHGTPMTLSELSSRLPSQPVNQDAL 61

Query: 1064 YRVMRYLVHMKLFTKASIDGELRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMAPWHYL 885
            YRVMRYLVH+KLFTK+SIDGELRYGLAPPAKFLVKGWD CM+GSIL + D DFMAP+HY+
Sbjct: 62   YRVMRYLVHIKLFTKSSIDGELRYGLAPPAKFLVKGWDNCMIGSILLMVDTDFMAPYHYI 121

Query: 884  KDGLA--GESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIFN 711
            KD +   G S TAFEKA G NIW Y+AEHPEKNQLFNE MAN+SRL MS L+K+C + F 
Sbjct: 122  KDEILNDGSSITAFEKASGTNIWEYLAEHPEKNQLFNEGMANESRLTMSGLMKDCSSTFE 181

Query: 710  XXXXXXXXXXXXXXXVRNIANAFPHIKCIVYDLPHVIADSPGYSEVHCVAGDMFKFIPKA 531
                           V+ IA AFP+IK  VYDLPHVIAD+PG +E++ V GDMFK+IP A
Sbjct: 182  GLTTLVDVGGGTGTTVKYIAKAFPNIKYTVYDLPHVIADAPGDTEINFVEGDMFKYIPNA 241

Query: 530  DAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTKMRLTLDLD 351
            DAI+MK +LHDWDD ECIEILKRCK+AVP +GGKVII+D+VLNV+SEHPYTKMRLTLDLD
Sbjct: 242  DAILMKGVLHDWDDNECIEILKRCKDAVPRDGGKVIIIDVVLNVKSEHPYTKMRLTLDLD 301

Query: 350  MMLNTGGKERTEEEWKNLIH-DAGYKGHKITQITAVQSVIEVYPY 219
            MMLN  GKERTEE+WK LI+ DAGYKG+KITQI+A+QSVIE YPY
Sbjct: 302  MMLNNKGKERTEEDWKKLIYDDAGYKGYKITQISALQSVIEAYPY 346


>dbj|BAM37634.1| O-methyltransferase [Eschscholzia californica]
          Length = 351

 Score =  451 bits (1160), Expect = e-124
 Identities = 220/345 (63%), Positives = 272/345 (78%), Gaps = 2/345 (0%)
 Frame = -1

Query: 1247 KDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLPSQPVNEDA 1068
            K + ++QAK+W  IYGFAESL+LK AV+L++A+ IHN+G  MTLSEL+S+LP QPV+ D 
Sbjct: 8    KIDQATQAKIWKHIYGFAESLILKSAVELEIADTIHNHGKPMTLSELASKLPVQPVDLDR 67

Query: 1067 LYRVMRYLVHMKLFTKASIDGE--LRYGLAPPAKFLVKGWDKCMVGSILAITDKDFMAPW 894
            LYRVMRYLVHMKLF K   + E  ++Y L PPAKFL+KGW+K MV +IL I DKDFM+PW
Sbjct: 68   LYRVMRYLVHMKLFKKQEDEDEESIKYSLLPPAKFLIKGWEKSMVSAILLINDKDFMSPW 127

Query: 893  HYLKDGLAGESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIF 714
            H+LK+GL   S  AF+KALG +IW YM+ +P+KN++FNE MA DSRL+ SALV ECGNIF
Sbjct: 128  HHLKEGLVNNSN-AFDKALGKSIWDYMSANPDKNKVFNEGMACDSRLVSSALVNECGNIF 186

Query: 713  NXXXXXXXXXXXXXXXVRNIANAFPHIKCIVYDLPHVIADSPGYSEVHCVAGDMFKFIPK 534
                            V+ ++ AFPHIKC +YDLPHVI+DSP    +  + G+MFK IP 
Sbjct: 187  QGIQTLVDVGGGTGTAVKAVSKAFPHIKCSIYDLPHVISDSPDIPNIVKIEGEMFKSIPS 246

Query: 533  ADAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTKMRLTLDL 354
            ADAI+MKCILHDW+D ECIEILK+CKEAVP EGGKVIIVDIVL+++S+HPY+K+RLTLDL
Sbjct: 247  ADAILMKCILHDWNDDECIEILKKCKEAVPKEGGKVIIVDIVLDMESKHPYSKVRLTLDL 306

Query: 353  DMMLNTGGKERTEEEWKNLIHDAGYKGHKITQITAVQSVIEVYPY 219
            DMMLNT GKERTEEEWK LI  AG+  +KITQI+A+QSVIE +PY
Sbjct: 307  DMMLNTEGKERTEEEWKKLIDGAGFASYKITQISALQSVIEAFPY 351


>gb|AAQ01669.1| (R,S)-norcoclaurine 6-O-methyltransferase [Papaver somniferum]
          Length = 346

 Score =  431 bits (1107), Expect = e-118
 Identities = 219/348 (62%), Positives = 265/348 (76%), Gaps = 3/348 (0%)
 Frame = -1

Query: 1253 VKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNNGTSMTLSELSSRLP-SQPVN 1077
            V K +  +QAK+W  IYGFAESLVLKCAVQL++A  +HNN   M+LSEL+S+LP +QPVN
Sbjct: 4    VSKIDQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNNVKPMSLSELASKLPVAQPVN 63

Query: 1076 EDALYRVMRYLVHMKLFTKASIDGEL-RYGLAPPAKFLVKGWDKCMVGSILAITDKDFMA 900
            ED L+R+MRYLVHM+LF    ID    +Y LAPPAK+L++GW+K MV SIL I DKDF+A
Sbjct: 64   EDRLFRIMRYLVHMELF---KIDATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLA 120

Query: 899  PWHYLKDGLAGESGTAFEKALGMNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGN 720
            PWH+L DGL G    AFEKALG +IW YM+ +PEKNQLFN AMA D+RL+ SAL  EC +
Sbjct: 121  PWHHLGDGLTGNCD-AFEKALGKSIWVYMSVNPEKNQLFNAAMACDTRLVTSALANECKS 179

Query: 719  IFNXXXXXXXXXXXXXXXV-RNIANAFPHIKCIVYDLPHVIADSPGYSEVHCVAGDMFKF 543
            IF+                 + I+ AFP IKC +YDLPHVIADSP    +  ++GDMFK 
Sbjct: 180  IFSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKS 239

Query: 542  IPKADAIMMKCILHDWDDKECIEILKRCKEAVPIEGGKVIIVDIVLNVQSEHPYTKMRLT 363
            IP ADAI MKCILHDW+D ECI+ILKRCKEA+P +GGKVIIVD+V+++ S HPY K+RLT
Sbjct: 240  IPSADAIFMKCILHDWNDDECIQILKRCKEALP-KGGKVIIVDVVIDMDSTHPYAKIRLT 298

Query: 362  LDLDMMLNTGGKERTEEEWKNLIHDAGYKGHKITQITAVQSVIEVYPY 219
            LDLDMMLNTGGKERT+EEWK L   AG+  HK+TQI+AVQSVIE YPY
Sbjct: 299  LDLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYPY 346