BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00001546
         (1605 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. diss...   876   0.0  
gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]                    501   e-139
sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltN...   491   e-136
sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcocla...   437   e-120
gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chi...   433   e-119

>dbj|BAF80448.1| corytuberine synthase [Coptis japonica var. dissecta]
          Length = 486

 Score =  876 bits (2263), Expect = 0.0
 Identities = 442/486 (90%), Positives = 445/486 (91%)
 Frame = +2

Query: 47   MDLQIALFSXXXXXXXXXXXXXXXXXXXXXGPHPWPLIGNLPTLFTNTEVPLHITLANMA 226
            MDLQIALFS                     GPHPWPLIGNLP LFTNTEVPLHITLANMA
Sbjct: 1    MDLQIALFSLIPVILVFILLLKPKYKNLPPGPHPWPLIGNLPILFTNTEVPLHITLANMA 60

Query: 227  RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC 406
            RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC
Sbjct: 61   RTHGPIMILWLGTQPTVMASTAEAAMEILKTHDRIFSARHIRMSFRLKHHIKYSLVWSDC 120

Query: 407  TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIEFLRSKEGQVVKISQFVFGTLLNI 586
            TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELI+FLRSKEGQVVKISQFVFGTLLNI
Sbjct: 121  TDYWKLLRKIVRTEIFSPKMLQAQSHVREQKVAELIDFLRSKEGQVVKISQFVFGTLLNI 180

Query: 587  LGNVVFSKDVFVYSDETDKGGIQNLIREMLMIGAEPNVAEFYPILEELDLQGLKKKCDDR 766
            LGNVVFSKDVFVY DETDKGGIQNLIREMLMIGAEPNVAEFYP LEELDLQGLKKKCD+R
Sbjct: 181  LGNVVFSKDVFVYGDETDKGGIQNLIREMLMIGAEPNVAEFYPSLEELDLQGLKKKCDER 240

Query: 767  FIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 946
            FIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE
Sbjct: 241  FIRVMKMWEGTVKERKANRNEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIE 300

Query: 947  WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMXXXXXXXXXXXX 1126
            WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAM            
Sbjct: 301  WVIAELIKSPKEMAKVRKELNEVVGTSTIKESDLPQLPYLQACIKEAMRLHPAAPFLLPR 360

Query: 1127 XXXETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIP 1306
               ETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTF PERFLESDVDFHGAHYQFIP
Sbjct: 361  RAAETCEVMGYTIPKNSQVLVNAYAIGRDPKSWKDPSTFWPERFLESDVDFHGAHYQFIP 420

Query: 1307 FGSGRRTCVGMPLATRTIPLIVGSLVHNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCV 1486
            FGSGRRTCVGMPLATRTIPLIVGSLVHNYD GLPGGNRPEDLKMNEMLSL+LAIDPSLCV
Sbjct: 421  FGSGRRTCVGMPLATRTIPLIVGSLVHNYDFGLPGGNRPEDLKMNEMLSLTLAIDPSLCV 480

Query: 1487 VPKARA 1504
            VPKARA
Sbjct: 481  VPKARA 486


>gb|AGC29949.1| CYP80G3 [Sinopodophyllum hexandrum]
          Length = 346

 Score =  501 bits (1290), Expect = e-139
 Identities = 249/346 (71%), Positives = 285/346 (82%)
 Frame = +2

Query: 464  MLQAQSHVREQKVAELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDK 643
            MLQ Q HVREQKVAEL+EFL+ KEGQVVKISQ VFGTLLNILGNV+FSKDVF Y    D 
Sbjct: 1    MLQVQEHVREQKVAELVEFLKGKEGQVVKISQLVFGTLLNILGNVIFSKDVFRYVG-ADG 59

Query: 644  GGIQNLIREMLMIGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANR 823
              +Q LI+EMLMIGA PN+AEF+PI EE DLQGLKK+C+ RF  VMKMW+GTV+ERK  R
Sbjct: 60   DKMQTLIKEMLMIGAAPNLAEFWPIFEEFDLQGLKKRCNQRFSEVMKMWKGTVEERKVKR 119

Query: 824  NEESKDMLDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKE 1003
            NEE KDMLDVLL NDFNDAQINA+FLETFGPGSETSSAT EW +AELIK+P  +AKVR+E
Sbjct: 120  NEEPKDMLDVLLLNDFNDAQINAVFLETFGPGSETSSATSEWALAELIKNPHILAKVRQE 179

Query: 1004 LNEVVGTSTIKESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQV 1183
            L++VVG ST+KESDLP LPYLQACIKE M               ETC VMGYTIPK++QV
Sbjct: 180  LDQVVGKSTVKESDLPHLPYLQACIKETMRLHPAAPFLLPHRAVETCPVMGYTIPKDTQV 239

Query: 1184 LVNAYAIGRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIP 1363
            LVN YAIGRDPK+WK+P+TF+PERFLESDVD++G H++FIPFG+GRR CVGMPLATRTIP
Sbjct: 240  LVNIYAIGRDPKTWKEPTTFKPERFLESDVDYYGKHFEFIPFGAGRRQCVGMPLATRTIP 299

Query: 1364 LIVGSLVHNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKAR 1501
            LIV +LV   D  LP G  P+DL MNE LSLSLA+DP+L V+PK+R
Sbjct: 300  LIVSNLVQTLDWSLPDGKLPQDLVMNESLSLSLALDPTLTVIPKSR 345


>sp|P47195.1|C80A1_BERST RecName: Full=Berbamunine synthase; AltName:
            Full=(S)-N-methylcoclaurine oxidase [C-O
            phenol-coupling]; AltName: Full=CYPLXXX; AltName:
            Full=Cytochrome P450 80 gi|642386|gb|AAC48987.1|
            cytochrome P-450 CYP80 [Berberis stolonifera]
          Length = 487

 Score =  491 bits (1263), Expect = e-136
 Identities = 240/455 (52%), Positives = 322/455 (70%), Gaps = 1/455 (0%)
 Frame = +2

Query: 140  PHPWPLIGNLPTLFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILKT 319
            P  WP++G+LP L +    P    ++N+A+ +GP++ L  G   ++ AST EAAME+L+T
Sbjct: 32   PPAWPIVGHLPDLISKNSPPFLDYMSNIAQKYGPLIHLKFGLHSSIFASTKEAAMEVLQT 91

Query: 320  HDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQK 499
            +D++ S R     FR+K HI YS++WSD   YWK  RKI+ TEIFS KMLQAQ   RE+ 
Sbjct: 92   NDKVLSGRQPLPCFRIKPHIDYSILWSDSNSYWKKGRKILHTEIFSQKMLQAQEKNRERV 151

Query: 500  VAELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDKGGIQNLIREMLM 679
               L+ F+ +K G VV++  ++FG  LN+LG+VVFSKDVF YSD++D+ G+  LI  MLM
Sbjct: 152  AGNLVNFIMTKVGDVVELRSWLFGCALNVLGHVVFSKDVFEYSDQSDEVGMDKLIHGMLM 211

Query: 680  IGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANRNEESKDMLDVLL 859
             G + +VA ++P+L   DL GLK+K D++F  ++K+WEG V  R+ANRN E KDMLDVL+
Sbjct: 212  TGGDFDVASYFPVLARFDLHGLKRKMDEQFKLLIKIWEGEVLARRANRNPEPKDMLDVLI 271

Query: 860  ANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVG-TSTIK 1036
            ANDFN+ QINA+F+ETFGPGS+T+S  IEW +A+LIK+P ++AK+R+EL+ VVG +ST+K
Sbjct: 272  ANDFNEHQINAMFMETFGPGSDTNSNIIEWALAQLIKNPDKLAKLREELDRVVGRSSTVK 331

Query: 1037 ESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAIGRDP 1216
            ES   +LPYLQAC+KE M               ETC+VMGYTIPK   V VNA+AIGRDP
Sbjct: 332  ESHFSELPYLQACVKETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDVHVNAHAIGRDP 391

Query: 1217 KSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLVHNYD 1396
            K WKDP  F+PERFL+SD++++G  +QFIPFGSGRR C G PLA R IPL++ SLVH + 
Sbjct: 392  KDWKDPLKFQPERFLDSDIEYNGKQFQFIPFGSGRRICPGRPLAVRIIPLVLASLVHAFG 451

Query: 1397 LGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKAR 1501
              LP G   E L M E+ +LSL +   L V+PK R
Sbjct: 452  WELPDGVPNEKLDMEELFTLSLCMAKPLRVIPKVR 486


>sp|Q9FXW4.1|C80B2_COPJA RecName: Full=Probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
            2; AltName: Full=Cytochrome P450 80B2
            gi|9971208|dbj|BAB12433.1|
            (S)-N-methylcoclaurine-3'-hydroxylase [Coptis japonica]
          Length = 488

 Score =  437 bits (1123), Expect = e-120
 Identities = 225/460 (48%), Positives = 307/460 (66%), Gaps = 4/460 (0%)
 Frame = +2

Query: 137  GPHPWPLIGNLPTLFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILK 316
            GP P P++GNL  L        H   A +A+ +G +  L LG+Q  V+AS+  AA EILK
Sbjct: 32   GPRPSPIVGNLLQLGDKP----HAEFAKLAQKYGELFSLKLGSQTVVVASSPAAAAEILK 87

Query: 317  THDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQ 496
            THD+I S R++  SFR+K H++ S+VWS+C D WKLLRK+ RTE+F+PKM+++QS +RE 
Sbjct: 88   THDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREA 147

Query: 497  KVAELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDKGGIQNL---IR 667
            K  E+++FLR KEG+VVKI + VFGTL+NI GN++FSKDVF   D T  GG   L   + 
Sbjct: 148  KAREMVKFLRGKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPT--GGSVELKEHLW 205

Query: 668  EMLMIGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANRNEESK-DM 844
            ++L +G   N A+++PI+ +LDL G ++   +   ++  +W   +KER+  +  ESK D 
Sbjct: 206  KLLDMGNSTNPADYFPIMGKLDLFGQRRAVAEVLQQIYDVWGVMLKERRGTKGSESKNDF 265

Query: 845  LDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGT 1024
            +DVLL    +D +INAL +E FG G+ETS++TIEW I EL K P  ++K+R EL  VVG 
Sbjct: 266  VDVLLNAGLDDQKINALLMELFGAGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGD 325

Query: 1025 STIKESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAI 1204
            +T+KESDLP LPYLQA +KE +               ETC VM YTIPK  Q++VNA+AI
Sbjct: 326  NTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAI 385

Query: 1205 GRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLV 1384
            GRDPK+W DP  F+PERFL SDVD+ G  ++ IPFG GRR C G+PLA++   LIV +LV
Sbjct: 386  GRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFSNLIVATLV 445

Query: 1385 HNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKARA 1504
             N++  LP G    +L M+E   L+L  DP L +V KARA
Sbjct: 446  QNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKARA 485


>gb|ABS19627.1| (S)-N-methylcoclaurine-3'-hydroxylase [Coptis chinensis]
          Length = 488

 Score =  433 bits (1114), Expect = e-119
 Identities = 223/459 (48%), Positives = 304/459 (66%), Gaps = 4/459 (0%)
 Frame = +2

Query: 137  GPHPWPLIGNLPTLFTNTEVPLHITLANMARTHGPIMILWLGTQPTVMASTAEAAMEILK 316
            GP P P++GNL  L            A +A+ +G +  L LG+Q  V+AS+  AA E+LK
Sbjct: 32   GPRPLPIVGNLLQLGDKPRAEF----AKLAQKYGQLFSLKLGSQTVVVASSPAAAAEVLK 87

Query: 317  THDRIFSARHIRMSFRLKHHIKYSLVWSDCTDYWKLLRKIVRTEIFSPKMLQAQSHVREQ 496
            THD+I S R++  SFR+K H++ S+VWS+C D WKLLRK+ RTE+F+PKM+++QS +RE 
Sbjct: 88   THDKILSGRYVFQSFRVKEHVENSIVWSECNDNWKLLRKVCRTELFTPKMIESQSEIREG 147

Query: 497  KVAELIEFLRSKEGQVVKISQFVFGTLLNILGNVVFSKDVFVYSDETDKGGIQNL---IR 667
            K  E+++FLRSKEG+VVKI + VFGTL+NI GN++FSKDVF   D T  GG   L   + 
Sbjct: 148  KAREMVKFLRSKEGEVVKIVEVVFGTLVNIFGNLIFSKDVFDLEDPT--GGSVELKEHLW 205

Query: 668  EMLMIGAEPNVAEFYPILEELDLQGLKKKCDDRFIRVMKMWEGTVKERKANRNEESK-DM 844
            ++L +G   N A+++PI+ + DL G ++   D   ++  +W   +KER+  +  ES  D 
Sbjct: 206  KLLEMGNSTNPADYFPIMGKYDLFGQRRAVADVLQQIYDVWGINLKERRGMKGSESNNDF 265

Query: 845  LDVLLANDFNDAQINALFLETFGPGSETSSATIEWVIAELIKSPKEMAKVRKELNEVVGT 1024
            +DVLL     D +INAL +E FG G+ETS++TIEW I EL K P  ++K+R EL  VVG 
Sbjct: 266  VDVLLNAGLEDQKINALLMELFGTGTETSASTIEWAITELTKKPLVVSKIRLELVNVVGD 325

Query: 1025 STIKESDLPQLPYLQACIKEAMXXXXXXXXXXXXXXXETCEVMGYTIPKNSQVLVNAYAI 1204
            +T+KESDLP LPYLQA +KE +               ETC VM YTIPK  Q++VNA+AI
Sbjct: 326  NTVKESDLPHLPYLQAFVKETLRLHPPTPLLLPRRALETCTVMNYTIPKECQIMVNAWAI 385

Query: 1205 GRDPKSWKDPSTFRPERFLESDVDFHGAHYQFIPFGSGRRTCVGMPLATRTIPLIVGSLV 1384
            GRDPK+W DP  F+PERFL SDVD+ G  ++ IPFG GRR C G+PLA++ I LIV +LV
Sbjct: 386  GRDPKTWDDPLNFKPERFLSSDVDYKGNDFELIPFGGGRRICPGLPLASQFISLIVATLV 445

Query: 1385 HNYDLGLPGGNRPEDLKMNEMLSLSLAIDPSLCVVPKAR 1501
             N++  LP G    +L M+E   L+L  DP L +V KAR
Sbjct: 446  QNFEWSLPQGMSTSELSMDEKFGLTLQKDPPLLIVLKAR 484