BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00000917
         (1245 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285915.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   479   e-162
ref|XP_003634474.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   469   e-159
emb|CBI18888.3| unnamed protein product [Vitis vinifera]              468   e-158
ref|NP_181956.2| cytoplasmic tRNA 2-thiolation protein 1 [Arabid...   472   e-156
ref|XP_002881955.1| hypothetical protein ARALYDRAFT_483552 [Arab...   471   e-156

>ref|XP_002285915.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like isoform 1
           [Vitis vinifera]
          Length = 356

 Score =  479 bits (1232), Expect(2) = e-162
 Identities = 231/255 (90%), Positives = 243/255 (95%), Gaps = 1/255 (0%)
 Frame = -2

Query: 944 GITGYRDDSLETVKRNELQYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 765
           GITGYRDDSLETVKRNE+QYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA
Sbjct: 102 GITGYRDDSLETVKRNEIQYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 161

Query: 764 LDRGAALLKVDKLATGHNADDIAETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKY 585
           LDRGAA+LKVDKLATGHNADDIAETVLLNILRGDIARL RCTSI+TGEDGPIPRCKPFKY
Sbjct: 162 LDRGAAMLKVDKLATGHNADDIAETVLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKY 221

Query: 584 TYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRI 405
           TYEKEIVMYAYFK+LDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDII+SGE FRI
Sbjct: 222 TYEKEIVMYAYFKRLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIRSGEKFRI 281

Query: 404 SNQTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPRLGIGRTRGL-NGHMTGVEKAD 228
           S   KMPEQGTCERCGYISSQKWCKACVLLEGLNRGLP+LGIGRTRGL NG     ++++
Sbjct: 282 STSAKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKLGIGRTRGLNNGLECDTKQSN 341

Query: 227 GAQSIQSKQCGTLDF 183
           G ++IQSKQCGTLDF
Sbjct: 342 GVKNIQSKQCGTLDF 356



 Score =  120 bits (300), Expect(2) = e-162
 Identities = 58/72 (80%), Positives = 64/72 (88%), Gaps = 1/72 (1%)
 Frame = -3

Query: 1219 IGEA-AKPKKTGGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKS 1043
            +GEA  KPKK G  QCSIC+QRR ALKRPKTLEQICRECFYAVFE+EIH+V+VDN+LFK 
Sbjct: 1    MGEAEVKPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKP 60

Query: 1042 GERIGIGASGGK 1007
            GERI IGASGGK
Sbjct: 61   GERIAIGASGGK 72


>ref|XP_003634474.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 1-like isoform 2
           [Vitis vinifera]
          Length = 363

 Score =  469 bits (1206), Expect(2) = e-159
 Identities = 229/262 (87%), Positives = 242/262 (92%), Gaps = 8/262 (3%)
 Frame = -2

Query: 944 GITGYRDDSLETVKRNELQYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 765
           GITGYRDDSLETVKRNE+QYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA
Sbjct: 102 GITGYRDDSLETVKRNEIQYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 161

Query: 764 LDRGAALLKVDKLATGHNADDIAETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKY 585
           LDRGAA+LKVDKLATGHNADDIAETVLLNILRGDIARL RCTSI+TGEDGPIPRCKPFKY
Sbjct: 162 LDRGAAMLKVDKLATGHNADDIAETVLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKY 221

Query: 584 TYEKEIVM-------YAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIK 426
           TYEKEI+        YAYFK+LDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDII+
Sbjct: 222 TYEKEILQNLNRIYTYAYFKRLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIR 281

Query: 425 SGESFRISNQTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPRLGIGRTRGL-NGHM 249
           SGE FRIS   KMPEQGTCERCGYISSQKWCKACVLLEGLNRGLP+LGIGRTRGL NG  
Sbjct: 282 SGEKFRISTSAKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKLGIGRTRGLNNGLE 341

Query: 248 TGVEKADGAQSIQSKQCGTLDF 183
              ++++G ++IQSKQCGTLDF
Sbjct: 342 CDTKQSNGVKNIQSKQCGTLDF 363



 Score =  120 bits (300), Expect(2) = e-159
 Identities = 58/72 (80%), Positives = 64/72 (88%), Gaps = 1/72 (1%)
 Frame = -3

Query: 1219 IGEA-AKPKKTGGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKS 1043
            +GEA  KPKK G  QCSIC+QRR ALKRPKTLEQICRECFYAVFE+EIH+V+VDN+LFK 
Sbjct: 1    MGEAEVKPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKP 60

Query: 1042 GERIGIGASGGK 1007
            GERI IGASGGK
Sbjct: 61   GERIAIGASGGK 72


>emb|CBI18888.3| unnamed protein product [Vitis vinifera]
          Length = 370

 Score =  468 bits (1203), Expect(2) = e-158
 Identities = 230/269 (85%), Positives = 243/269 (90%), Gaps = 15/269 (5%)
 Frame = -2

Query: 944 GITGYRDDSLETVKRNELQYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 765
           GITGYRDDSLETVKRNE+QYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA
Sbjct: 102 GITGYRDDSLETVKRNEIQYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 161

Query: 764 LDRGAALLKVDKLATGHNADDIAETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKY 585
           LDRGAA+LKVDKLATGHNADDIAETVLLNILRGDIARL RCTSI+TGEDGPIPRCKPFKY
Sbjct: 162 LDRGAAMLKVDKLATGHNADDIAETVLLNILRGDIARLSRCTSIITGEDGPIPRCKPFKY 221

Query: 584 TYEKEIVM--------------YAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPR 447
           TYEKEIV+              YAYFK+LDYFSTECIYSPNAYRGFAREFIKDLERIRPR
Sbjct: 222 TYEKEIVIYPYSFLQNLNRIYTYAYFKRLDYFSTECIYSPNAYRGFAREFIKDLERIRPR 281

Query: 446 AILDIIKSGESFRISNQTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPRLGIGRTR 267
           AILDII+SGE FRIS   KMPEQGTCERCGYISSQKWCKACVLLEGLNRGLP+LGIGRTR
Sbjct: 282 AILDIIRSGEKFRISTSAKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKLGIGRTR 341

Query: 266 GL-NGHMTGVEKADGAQSIQSKQCGTLDF 183
           GL NG     ++++G ++IQSKQCGTLDF
Sbjct: 342 GLNNGLECDTKQSNGVKNIQSKQCGTLDF 370



 Score =  120 bits (300), Expect(2) = e-158
 Identities = 58/72 (80%), Positives = 64/72 (88%), Gaps = 1/72 (1%)
 Frame = -3

Query: 1219 IGEA-AKPKKTGGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKS 1043
            +GEA  KPKK G  QCSIC+QRR ALKRPKTLEQICRECFYAVFE+EIH+V+VDN+LFK 
Sbjct: 1    MGEAEVKPKKAGPVQCSICSQRRAALKRPKTLEQICRECFYAVFEEEIHRVVVDNQLFKP 60

Query: 1042 GERIGIGASGGK 1007
            GERI IGASGGK
Sbjct: 61   GERIAIGASGGK 72


>ref|NP_181956.2| cytoplasmic tRNA 2-thiolation protein 1 [Arabidopsis thaliana]
           gi|226711456|sp|O64862.2|CTU1_ARATH RecName:
           Full=Cytoplasmic tRNA 2-thiolation protein 1; AltName:
           Full=Cytoplasmic tRNA adenylyltransferase 1
           gi|26449617|dbj|BAC41934.1| unknown protein [Arabidopsis
           thaliana] gi|30725380|gb|AAP37712.1| At2g44270
           [Arabidopsis thaliana] gi|330255305|gb|AEC10399.1|
           cytoplasmic tRNA 2-thiolation protein 1 [Arabidopsis
           thaliana]
          Length = 355

 Score =  472 bits (1214), Expect(2) = e-156
 Identities = 229/256 (89%), Positives = 239/256 (93%), Gaps = 2/256 (0%)
 Frame = -2

Query: 944 GITGYRDDSLETVKRNELQYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 765
           GITGYRDDSLETVKRNE+QYGLPLK+VSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA
Sbjct: 100 GITGYRDDSLETVKRNEVQYGLPLKIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 159

Query: 764 LDRGAALLKVDKLATGHNADDIAETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKY 585
           LDRGAALLKV+KL TGHNADDIAETVLLNILRGDIARL RCTSI TGEDGPIPRCKPFKY
Sbjct: 160 LDRGAALLKVEKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKY 219

Query: 584 TYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRI 405
           TYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGE FRI
Sbjct: 220 TYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRI 279

Query: 404 SNQTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPRLGIGRTRGLNGHMTGVEKADG 225
           +  TKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLP++GIGR RG+NG      K  G
Sbjct: 280 ATTTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKMGIGRPRGVNGDHNKETKKPG 339

Query: 224 --AQSIQSKQCGTLDF 183
             A+SI+SKQCG+LDF
Sbjct: 340 SVAKSIESKQCGSLDF 355



 Score =  107 bits (268), Expect(2) = e-156
 Identities = 49/69 (71%), Positives = 55/69 (79%)
 Frame = -3

Query: 1213 EAAKPKKTGGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKSGER 1034
            EA   K    R C +CN RRP LKRPKTL+QICRECFY VFE+EIH+VIV N+LFKSGER
Sbjct: 2    EAKNKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGER 61

Query: 1033 IGIGASGGK 1007
            + IGASGGK
Sbjct: 62   VAIGASGGK 70


>ref|XP_002881955.1| hypothetical protein ARALYDRAFT_483552 [Arabidopsis lyrata subsp.
           lyrata] gi|297327794|gb|EFH58214.1| hypothetical protein
           ARALYDRAFT_483552 [Arabidopsis lyrata subsp. lyrata]
          Length = 355

 Score =  471 bits (1212), Expect(2) = e-156
 Identities = 229/256 (89%), Positives = 239/256 (93%), Gaps = 2/256 (0%)
 Frame = -2

Query: 944 GITGYRDDSLETVKRNELQYGLPLKVVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 765
           GITGYRDDSLETVKRNE+QYGLPL++VSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA
Sbjct: 100 GITGYRDDSLETVKRNEVQYGLPLQIVSYKDLYGWTMDEIVKMIGLKNNCTFCGVFRRQA 159

Query: 764 LDRGAALLKVDKLATGHNADDIAETVLLNILRGDIARLGRCTSIVTGEDGPIPRCKPFKY 585
           LDRGAALLKVDKL TGHNADDIAETVLLNILRGDIARL RCTSI TGEDGPIPRCKPFKY
Sbjct: 160 LDRGAALLKVDKLVTGHNADDIAETVLLNILRGDIARLSRCTSITTGEDGPIPRCKPFKY 219

Query: 584 TYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGESFRI 405
           TYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGE FRI
Sbjct: 220 TYEKEIVMYAYFKKLDYFSTECIYSPNAYRGFAREFIKDLERIRPRAILDIIKSGEDFRI 279

Query: 404 SNQTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPRLGIGRTRGLNGHMTGVEKADG 225
           +  TKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLP++GIGR RG+NG      K  G
Sbjct: 280 ATTTKMPEQGTCERCGYISSQKWCKACVLLEGLNRGLPKMGIGRPRGVNGDHKMETKKPG 339

Query: 224 --AQSIQSKQCGTLDF 183
             A+SI+SKQCG+LDF
Sbjct: 340 SVAKSIESKQCGSLDF 355



 Score =  107 bits (268), Expect(2) = e-156
 Identities = 49/69 (71%), Positives = 55/69 (79%)
 Frame = -3

Query: 1213 EAAKPKKTGGRQCSICNQRRPALKRPKTLEQICRECFYAVFEDEIHKVIVDNKLFKSGER 1034
            EA   K    R C +CN RRP LKRPKTL+QICRECFY VFE+EIH+VIV N+LFKSGER
Sbjct: 2    EAKSKKAVASRLCCLCNLRRPVLKRPKTLQQICRECFYEVFEEEIHQVIVQNRLFKSGER 61

Query: 1033 IGIGASGGK 1007
            + IGASGGK
Sbjct: 62   VAIGASGGK 70