BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00000712
         (1542 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q39522.1|SMT_COPJA RecName: Full=(S)-scoulerine 9-O-methyltra...   743   0.0  
gb|ACL31653.1| (S)-scoulerine 9-O-methyltransferase [Coptis chin...   657   0.0  
gb|AAU20770.1| (S)-scoulerine 9-O-methyltransferase [Thalictrum ...   631   e-178
gb|AFB74611.1| O-methyltransferase 1 [Papaver somniferum]             427   e-117
gb|AFK73709.1| scoulerine-9-O-methyltransferase [Papaver somnife...   426   e-116

>sp|Q39522.1|SMT_COPJA RecName: Full=(S)-scoulerine 9-O-methyltransferase
            gi|758580|dbj|BAA06192.1|
            S-adenosyl-L-methionine:scoulerine 9-O-methyltransferase
            [Coptis japonica]
          Length = 381

 Score =  743 bits (1917), Expect = 0.0
 Identities = 374/381 (98%), Positives = 378/381 (99%)
 Frame = +2

Query: 86   MCTSLSELKYPVFSTKSRLLLEVALRTSVDMAAQEGVNYLSGLGLSRLICLPMALRAAIE 265
            MCTSLSELK PVFSTK +LLLE ALRTSVDMAAQEGVNYLSGLGLSRLICLPMALRAAIE
Sbjct: 1    MCTSLSELKCPVFSTKRKLLLEFALRTSVDMAAQEGVNYLSGLGLSRLICLPMALRAAIE 60

Query: 266  LNVFEIISQAGPDAQLSPSDIVAKIPTKNPSAAISLDRILRMLGASSILSVSTTKSGRVY 445
            LNVFEIISQAGPDAQLSPSDIVAKIPTKNPSAAISLDRILRMLGASSILSVSTTKSGRVY
Sbjct: 61   LNVFEIISQAGPDAQLSPSDIVAKIPTKNPSAAISLDRILRMLGASSILSVSTTKSGRVY 120

Query: 446  GLNEESRCLVASEDKVSVVPMLLFTSDKAVVESFYNIKDVVLEEGVIPFDRTHGMDFFQY 625
            GLNEESRCLVASEDKVSVVPMLLFTSDKAVVESFYNIKDVVLEEGVIPFDRTHGMDFFQY
Sbjct: 121  GLNEESRCLVASEDKVSVVPMLLFTSDKAVVESFYNIKDVVLEEGVIPFDRTHGMDFFQY 180

Query: 626  AGKEQRVNKSFNQAMGAGSTIAFDEVFEVYKGFDNLKELVDVGGGIGTSLSNIVAKYPHI 805
            AGKE+RVNKSFNQAMGAGSTIAFDEVF+VYKGFDNLKELVDVGGGIGTSLSNIVAK+PHI
Sbjct: 181  AGKEERVNKSFNQAMGAGSTIAFDEVFKVYKGFDNLKELVDVGGGIGTSLSNIVAKHPHI 240

Query: 806  RGINFELPHVIGDAPDYPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWK 985
            RGINFELPHVIGDAPDYPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWK
Sbjct: 241  RGINFELPHVIGDAPDYPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWK 300

Query: 986  ALPENGTVIVIEFVLPQVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFA 1165
            ALPENGTVIVIEFVLPQVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFA
Sbjct: 301  ALPENGTVIVIEFVLPQVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFA 360

Query: 1166 ETKFFPISQGLHVMEFHKINC 1228
            ETKFFPISQGLHVMEFHKINC
Sbjct: 361  ETKFFPISQGLHVMEFHKINC 381


>gb|ACL31653.1| (S)-scoulerine 9-O-methyltransferase [Coptis chinensis]
          Length = 350

 Score =  657 bits (1694), Expect = 0.0
 Identities = 334/351 (95%), Positives = 337/351 (96%)
 Frame = +2

Query: 176  MAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAGPDAQLSPSDIVAKIPTKNP 355
            MAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAG         I ++ P +NP
Sbjct: 1    MAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAGQMLNYH-HQISSQNPHENP 59

Query: 356  SAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVASEDKVSVVPMLLFTSDKAV 535
            SAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVASEDKVSVVPMLLFT DKAV
Sbjct: 60   SAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVASEDKVSVVPMLLFTPDKAV 119

Query: 536  VESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMGAGSTIAFDEVFEVY 715
            VESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMGAGSTIAFDEVFEVY
Sbjct: 120  VESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMGAGSTIAFDEVFEVY 179

Query: 716  KGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPDYPGVEHVPGDMFEG 895
            KGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPDYPGVEHVPGDMFEG
Sbjct: 180  KGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPDYPGVEHVPGDMFEG 239

Query: 896  VPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLPQVLGNNAESFNALT 1075
            VPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLPQVLGN AESFNALT
Sbjct: 240  VPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLPQVLGNIAESFNALT 299

Query: 1076 PDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEFHKINC 1228
            PDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEFHKINC
Sbjct: 300  PDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEFHKINC 350


>gb|AAU20770.1| (S)-scoulerine 9-O-methyltransferase [Thalictrum flavum subsp.
            glaucum]
          Length = 355

 Score =  631 bits (1627), Expect = e-178
 Identities = 312/348 (89%), Positives = 332/348 (95%)
 Frame = +2

Query: 176  MAAQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIISQAGPDAQLSPSDIVAKIPTKNP 355
            MA QEGVNYLSGLGLSRLICLPMALRAAIELNVFEII QAGP+AQLSP++IVAKIPTKNP
Sbjct: 1    MALQEGVNYLSGLGLSRLICLPMALRAAIELNVFEIIFQAGPEAQLSPAEIVAKIPTKNP 60

Query: 356  SAAISLDRILRMLGASSILSVSTTKSGRVYGLNEESRCLVASEDKVSVVPMLLFTSDKAV 535
            +AAI+LDRILRMLGASSILSV+T K GRVYGL EESRCLVA ++ VSVVPMLLFTSDKAV
Sbjct: 61   NAAIALDRILRMLGASSILSVTTMKDGRVYGLTEESRCLVADKNGVSVVPMLLFTSDKAV 120

Query: 536  VESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMGAGSTIAFDEVFEVY 715
            VESFYNIKDVVLEEGVIPFDRTHGMDFF YAGKEQ VNKSFNQAMGAGSTIAFDEVF+VY
Sbjct: 121  VESFYNIKDVVLEEGVIPFDRTHGMDFFAYAGKEQSVNKSFNQAMGAGSTIAFDEVFKVY 180

Query: 716  KGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPDYPGVEHVPGDMFEG 895
            KGF +LKELV+VGGGIGTSLSNI+ KYPHI+GINFELPHVI DAP+YPGVEH+ G+MFEG
Sbjct: 181  KGFHDLKELVNVGGGIGTSLSNIIFKYPHIKGINFELPHVIADAPNYPGVEHIAGNMFEG 240

Query: 896  VPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLPQVLGNNAESFNALT 1075
            VPNAQNILLKWVLHDWDD+RSIKIL+NCWKALPE GTVIV+EFVLPQ+LGNNAESFNALT
Sbjct: 241  VPNAQNILLKWVLHDWDDERSIKILQNCWKALPEGGTVIVVEFVLPQILGNNAESFNALT 300

Query: 1076 PDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEFHK 1219
            PDLLMM LNPGGKERTT EFDGLAKAAGFAETKFFPISQGLHVMEFHK
Sbjct: 301  PDLLMMTLNPGGKERTTTEFDGLAKAAGFAETKFFPISQGLHVMEFHK 348


>gb|AFB74611.1| O-methyltransferase 1 [Papaver somniferum]
          Length = 390

 Score =  427 bits (1099), Expect = e-117
 Identities = 222/362 (61%), Positives = 267/362 (73%), Gaps = 16/362 (4%)
 Frame = +2

Query: 182  AQEGVNYLSGLG-LSRLICLPMALRAAIELNVFEIISQAGPDAQLSPSDIVAKIPT--KN 352
            +  GV YLS    L +LIC+PMALRAA+ELNVF++IS+ G DA++S S+I +K+P    N
Sbjct: 29   SSNGVCYLSETANLGKLICIPMALRAAMELNVFQLISKFGTDAKVSASEIASKMPNAKNN 88

Query: 353  PSAAISLDRILRMLGASSILSVSTTKSG------------RVYGLNEESRCLVA-SEDKV 493
            P AA+ LDRILR+LGASSILSVSTTK              ++YGL   S CLV   ED V
Sbjct: 89   PEAAMYLDRILRLLGASSILSVSTTKKSINRGGDDVVVHEKLYGLTNSSCCLVPRQEDGV 148

Query: 494  SVVPMLLFTSDKAVVESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMG 673
            S+V  LLFTSDK VV+SF+ +K VV E+  +PF+  HG   F+YA  E R+N+ FN  M 
Sbjct: 149  SLVEELLFTSDKVVVDSFFKLKCVVEEKDSVPFEVAHGAKIFEYAATEPRMNQVFNDGMA 208

Query: 674  AGSTIAFDEVFEVYKGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPD 853
              S + F+ VF VY GF ++KEL+DVGGGIGTS+S IVAKYP IRG+NF+LPHVI  AP 
Sbjct: 209  VFSIVVFEAVFRVYDGFLDMKELLDVGGGIGTSVSKIVAKYPLIRGVNFDLPHVISVAPQ 268

Query: 854  YPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLP 1033
            YPGVEHV GDMFE VP  QN+LLKWVLHDW D+R +K+LKNCW +LP  G V++IEFVLP
Sbjct: 269  YPGVEHVAGDMFEEVPKGQNMLLKWVLHDWGDERCVKLLKNCWNSLPVGGKVLIIEFVLP 328

Query: 1034 QVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEF 1213
              LGNNAESFNAL PDLL+MALNPGGKERT  E+D L KAAGF +T   PIS GLHV+EF
Sbjct: 329  NELGNNAESFNALIPDLLLMALNPGGKERTISEYDDLGKAAGFIKTIPIPISNGLHVIEF 388

Query: 1214 HK 1219
            HK
Sbjct: 389  HK 390


>gb|AFK73709.1| scoulerine-9-O-methyltransferase [Papaver somniferum]
          Length = 390

 Score =  426 bits (1094), Expect = e-116
 Identities = 221/362 (61%), Positives = 266/362 (73%), Gaps = 16/362 (4%)
 Frame = +2

Query: 182  AQEGVNYLSGLG-LSRLICLPMALRAAIELNVFEIISQAGPDAQLSPSDIVAKIPT--KN 352
            +  GV YLS    L +LIC+PMALRAA+ELNVF++IS+ G DA++S S+I +K+P    N
Sbjct: 29   SSNGVCYLSETANLGKLICIPMALRAAMELNVFQLISKFGTDAKVSASEIASKMPNAKNN 88

Query: 353  PSAAISLDRILRMLGASSILSVSTTKSG------------RVYGLNEESRCLVA-SEDKV 493
            P AA+ LDRILR+LGASSILSVSTTK              ++YGL   S CLV   ED V
Sbjct: 89   PEAAMYLDRILRLLGASSILSVSTTKKSINRGGDDVVVHEKLYGLTNSSCCLVPRQEDGV 148

Query: 494  SVVPMLLFTSDKAVVESFYNIKDVVLEEGVIPFDRTHGMDFFQYAGKEQRVNKSFNQAMG 673
            S+V  LLFTSDK VV+SF+ +K VV E+  +PF+  HG   F+YA  E R+N+ FN  M 
Sbjct: 149  SLVEELLFTSDKVVVDSFFKLKCVVEEKDSVPFEVAHGAKIFEYAATEPRMNQVFNDGMA 208

Query: 674  AGSTIAFDEVFEVYKGFDNLKELVDVGGGIGTSLSNIVAKYPHIRGINFELPHVIGDAPD 853
              S + F+ VF  Y GF ++KEL+DVGGGIGTS+S IVAKYP IRG+NF+LPHVI  AP 
Sbjct: 209  VFSIVVFEAVFRFYDGFLDMKELLDVGGGIGTSVSKIVAKYPLIRGVNFDLPHVISVAPQ 268

Query: 854  YPGVEHVPGDMFEGVPNAQNILLKWVLHDWDDDRSIKILKNCWKALPENGTVIVIEFVLP 1033
            YPGVEHV GDMFE VP  QN+LLKWVLHDW D+R +K+LKNCW +LP  G V++IEFVLP
Sbjct: 269  YPGVEHVAGDMFEEVPKGQNMLLKWVLHDWGDERCVKLLKNCWNSLPVGGKVLIIEFVLP 328

Query: 1034 QVLGNNAESFNALTPDLLMMALNPGGKERTTIEFDGLAKAAGFAETKFFPISQGLHVMEF 1213
              LGNNAESFNAL PDLL+MALNPGGKERT  E+D L KAAGF +T   PIS GLHV+EF
Sbjct: 329  NELGNNAESFNALIPDLLLMALNPGGKERTISEYDDLGKAAGFIKTIPIPISNGLHVIEF 388

Query: 1214 HK 1219
            HK
Sbjct: 389  HK 390