BLASTX nr result
ID: Coptis25_contig00000504
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis25_contig00000504
(1492 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002269753.1| PREDICTED: subtilisin-like protease-like [Vi... 609 e-172
emb|CBI19918.3| unnamed protein product [Vitis vinifera] 607 e-171
ref|NP_564107.1| Subtilisin-like serine endopeptidase-like prote... 559 e-157
gb|AAM65424.1| subtilisin-like serine protease [Arabidopsis thal... 558 e-156
dbj|BAJ53097.1| JHL20J20.3 [Jatropha curcas] 558 e-156
>ref|XP_002269753.1| PREDICTED: subtilisin-like protease-like [Vitis vinifera]
Length = 768
Score = 609 bits (1571), Expect = e-172
Identities = 318/500 (63%), Positives = 382/500 (76%), Gaps = 3/500 (0%)
Frame = +2
Query: 2 ADKGEKKSVYIVYTGAATSRLSSSRDNHIQILSSVLKRNDDAEKRLVHTYSRGFSGCAVW 181
AD+ + +YIVY GAATS S R +H QILSS+LKR +A LVH+Y GFSG A
Sbjct: 23 ADEMKNDRIYIVYMGAATSSEGSYRYDHAQILSSLLKRKANA---LVHSYRHGFSGFAAH 79
Query: 182 LTEEEARLIGQRSDVVSVFVDPIFKLHTTRSWDFLQY--DLETYSTPTLNSNALSDGY-D 352
LTEEEAR I Q+ VVSVF DP+ +LHTTRSWDFL Y DLET S P + ++ S G D
Sbjct: 80 LTEEEARSIAQKPGVVSVFEDPVLQLHTTRSWDFLHYQTDLETDSKPGSDGDSQSSGQAD 139
Query: 353 TIIGVFDTGIWPESDSFNDKDMGSIPSGWKGTCMEGNDFKAFKCNRKLIGARYYKETIND 532
TIIG+ DTGIWPES+SF+DK MG +PS W+GTCME ND +FKCNRKLIGARYY ND
Sbjct: 140 TIIGILDTGIWPESESFSDKTMGPVPSRWRGTCMESNDVDSFKCNRKLIGARYY----ND 195
Query: 533 EDDRPSKHLNDEGNTARDSQGHGTHTASTAAGSYVSNASYYGLAAGTAKGGSPGSRLAIY 712
D + +TARD GHGTH ASTAAG+ + + SYYGLA+GTAKGGSPGSR+A+Y
Sbjct: 196 SDAASAVP-----HTARDMIGHGTHVASTAAGNSLPDVSYYGLASGTAKGGSPGSRIAMY 250
Query: 713 KVCASGGCRGSAILAAFDDAISDGVNVISLSLGAAAYMRPNYSNDPIAIGAFHAVEKGIT 892
+VC GCRGS+ILAAFDDAISDGV+V+SLSLG++A +S DPIAIGA+HAV KGIT
Sbjct: 251 RVCTFFGCRGSSILAAFDDAISDGVDVLSLSLGSSAVFELEFSTDPIAIGAYHAVAKGIT 310
Query: 893 VVCSAGNDGPTSASVVNAAPWILTVAATTIDRDFESDVIYGENNVIKGGGINFSNLKRSP 1072
VVCSAGNDGP+ +VVN APWILTV ATTIDRDFESDV+ G N VIKG GINF+N+K+SP
Sbjct: 311 VVCSAGNDGPSPQTVVNIAPWILTVGATTIDRDFESDVVLGGNKVIKGEGINFANIKKSP 370
Query: 1073 AHPLIYGKSAKTSSSTDNQASNCDPNALDGNKIKGNIVLCHHSDGSYSKREIRDGVEDLE 1252
A+PLIYG SAK++SS + A NC PN+L +KIKG IVLC + DG Y++ E + V+ L
Sbjct: 371 AYPLIYGSSAKSNSSKVDDARNCKPNSLGEDKIKGRIVLCDNDDGEYTQTEKLEEVKRLG 430
Query: 1253 AIGLVLINDLERSVPSPSGNFPMTIISSKEAAALLSYINSTKNPVATISPTVTVTKYKPA 1432
+GL+LI D R+V S G FP+T+I+SK+A+ +LSYINST+NPVATI TV+V +YKPA
Sbjct: 431 GVGLILIEDETRAVASRYGAFPLTVITSKDASEILSYINSTRNPVATILATVSVEQYKPA 490
Query: 1433 PQVAYFSSRGPSDQTKNLLK 1492
P VAYFSSRGPS TKNLLK
Sbjct: 491 PAVAYFSSRGPSYATKNLLK 510
>emb|CBI19918.3| unnamed protein product [Vitis vinifera]
Length = 743
Score = 607 bits (1564), Expect = e-171
Identities = 316/492 (64%), Positives = 378/492 (76%), Gaps = 3/492 (0%)
Frame = +2
Query: 26 VYIVYTGAATSRLSSSRDNHIQILSSVLKRNDDAEKRLVHTYSRGFSGCAVWLTEEEARL 205
+YIVY GAATS S R +H QILSS+LKR +A LVH+Y GFSG A LTEEEAR
Sbjct: 6 IYIVYMGAATSSEGSYRYDHAQILSSLLKRKANA---LVHSYRHGFSGFAAHLTEEEARS 62
Query: 206 IGQRSDVVSVFVDPIFKLHTTRSWDFLQY--DLETYSTPTLNSNALSDGY-DTIIGVFDT 376
I Q+ VVSVF DP+ +LHTTRSWDFL Y DLET S P + ++ S G DTIIG+ DT
Sbjct: 63 IAQKPGVVSVFEDPVLQLHTTRSWDFLHYQTDLETDSKPGSDGDSQSSGQADTIIGILDT 122
Query: 377 GIWPESDSFNDKDMGSIPSGWKGTCMEGNDFKAFKCNRKLIGARYYKETINDEDDRPSKH 556
GIWPES+SF+DK MG +PS W+GTCME ND +FKCNRKLIGARYY ND D +
Sbjct: 123 GIWPESESFSDKTMGPVPSRWRGTCMESNDVDSFKCNRKLIGARYY----NDSDAASAVP 178
Query: 557 LNDEGNTARDSQGHGTHTASTAAGSYVSNASYYGLAAGTAKGGSPGSRLAIYKVCASGGC 736
+TARD GHGTH ASTAAG+ + + SYYGLA+GTAKGGSPGSR+A+Y+VC GC
Sbjct: 179 -----HTARDMIGHGTHVASTAAGNSLPDVSYYGLASGTAKGGSPGSRIAMYRVCTFFGC 233
Query: 737 RGSAILAAFDDAISDGVNVISLSLGAAAYMRPNYSNDPIAIGAFHAVEKGITVVCSAGND 916
RGS+ILAAFDDAISDGV+V+SLSLG++A +S DPIAIGA+HAV KGITVVCSAGND
Sbjct: 234 RGSSILAAFDDAISDGVDVLSLSLGSSAVFELEFSTDPIAIGAYHAVAKGITVVCSAGND 293
Query: 917 GPTSASVVNAAPWILTVAATTIDRDFESDVIYGENNVIKGGGINFSNLKRSPAHPLIYGK 1096
GP+ +VVN APWILTV ATTIDRDFESDV+ G N VIKG GINF+N+K+SPA+PLIYG
Sbjct: 294 GPSPQTVVNIAPWILTVGATTIDRDFESDVVLGGNKVIKGEGINFANIKKSPAYPLIYGS 353
Query: 1097 SAKTSSSTDNQASNCDPNALDGNKIKGNIVLCHHSDGSYSKREIRDGVEDLEAIGLVLIN 1276
SAK++SS + A NC PN+L +KIKG IVLC + DG Y++ E + V+ L +GL+LI
Sbjct: 354 SAKSNSSKVDDARNCKPNSLGEDKIKGRIVLCDNDDGEYTQTEKLEEVKRLGGVGLILIE 413
Query: 1277 DLERSVPSPSGNFPMTIISSKEAAALLSYINSTKNPVATISPTVTVTKYKPAPQVAYFSS 1456
D R+V S G FP+T+I+SK+A+ +LSYINST+NPVATI TV+V +YKPAP VAYFSS
Sbjct: 414 DETRAVASRYGAFPLTVITSKDASEILSYINSTRNPVATILATVSVEQYKPAPAVAYFSS 473
Query: 1457 RGPSDQTKNLLK 1492
RGPS TKNLLK
Sbjct: 474 RGPSYATKNLLK 485
>ref|NP_564107.1| Subtilisin-like serine endopeptidase-like protein [Arabidopsis
thaliana] gi|8778982|gb|AAF79897.1|AC022472_6 Contains
similarity to p69c gene from Lycopersicon esculentum
gb|Y17277 and is a member of subtilase family PF|00082.
ESTs gb|T22485, gb|R65370, gb|AA651071 come from this
gene [Arabidopsis thaliana] gi|110737651|dbj|BAF00765.1|
hypothetical protein [Arabidopsis thaliana]
gi|332191824|gb|AEE29945.1| Subtilisin-like serine
endopeptidase-like protein [Arabidopsis thaliana]
Length = 769
Score = 559 bits (1440), Expect = e-157
Identities = 286/489 (58%), Positives = 354/489 (72%)
Frame = +2
Query: 26 VYIVYTGAATSRLSSSRDNHIQILSSVLKRNDDAEKRLVHTYSRGFSGCAVWLTEEEARL 205
VYIVY G+A+S +++R I I + +R +D L+HTY GFSG A LT EEA++
Sbjct: 35 VYIVYMGSASSAANANRAQ-ILINTMFKRRAND----LLHTYKHGFSGFAARLTAEEAKV 89
Query: 206 IGQRSDVVSVFVDPIFKLHTTRSWDFLQYDLETYSTPTLNSNALSDGYDTIIGVFDTGIW 385
I ++ VVSVF DP F+LHTT SWDFL+Y S+A YD+I+G+ DTGIW
Sbjct: 90 IAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVKVDSGPPSSASDGSYDSIVGILDTGIW 149
Query: 386 PESDSFNDKDMGSIPSGWKGTCMEGNDFKAFKCNRKLIGARYYKETINDEDDRPSKHLND 565
PES+SFNDKDMG IPS WKGTCME DFK+ CNRK+IGARYYK N +DD
Sbjct: 150 PESESFNDKDMGPIPSRWKGTCMEAKDFKSSNCNRKIIGARYYK---NPDDD-------S 199
Query: 566 EGNTARDSQGHGTHTASTAAGSYVSNASYYGLAAGTAKGGSPGSRLAIYKVCASGGCRGS 745
E T RD GHG+H +ST AGS V NASYYG+A+GTAKGGS +R+A+YKVC GGC GS
Sbjct: 200 EYYTTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGS 259
Query: 746 AILAAFDDAISDGVNVISLSLGAAAYMRPNYSNDPIAIGAFHAVEKGITVVCSAGNDGPT 925
+ILAAFDDAI+DGV+V+SLSLGA AY R + + DPIAIGAFHAVE+GI V+CSAGNDGP
Sbjct: 260 SILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPD 319
Query: 926 SASVVNAAPWILTVAATTIDRDFESDVIYGENNVIKGGGINFSNLKRSPAHPLIYGKSAK 1105
+V N APWI+TVAA TIDRDFESDV+ G N VIKG GI+FSN+ +SP +PLI+GKSAK
Sbjct: 320 GGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGIHFSNVSKSPVYPLIHGKSAK 379
Query: 1106 TSSSTDNQASNCDPNALDGNKIKGNIVLCHHSDGSYSKREIRDGVEDLEAIGLVLINDLE 1285
++ +++ A CD ++LD K+KG IVLC + GSY RD V+ G V ++D
Sbjct: 380 SADASEGSARACDSDSLDQEKVKGKIVLCENVGGSYYASSARDEVKSKGGTGCVFVDDRT 439
Query: 1286 RSVPSPSGNFPMTIISSKEAAALLSYINSTKNPVATISPTVTVTKYKPAPQVAYFSSRGP 1465
R+V S G+FP T+I SKEAA + SY+NSTK+PVATI PT TV K+ PAP VAYFSSRGP
Sbjct: 440 RAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATILPTATVEKFTPAPAVAYFSSRGP 499
Query: 1466 SDQTKNLLK 1492
S T+++LK
Sbjct: 500 SSLTRSILK 508
>gb|AAM65424.1| subtilisin-like serine protease [Arabidopsis thaliana]
Length = 769
Score = 558 bits (1439), Expect = e-156
Identities = 286/489 (58%), Positives = 354/489 (72%)
Frame = +2
Query: 26 VYIVYTGAATSRLSSSRDNHIQILSSVLKRNDDAEKRLVHTYSRGFSGCAVWLTEEEARL 205
VYIVY G+A+S +++R I I + +R +D L+HTY GFSG A LT EEA++
Sbjct: 35 VYIVYMGSASSAANANRAQ-ILINTMFKRRAND----LLHTYKHGFSGFAARLTAEEAKV 89
Query: 206 IGQRSDVVSVFVDPIFKLHTTRSWDFLQYDLETYSTPTLNSNALSDGYDTIIGVFDTGIW 385
I ++ VVSVF DP F+LHTT SWDFL+Y S+A YD+I+G+ DTGIW
Sbjct: 90 IAKKPGVVSVFPDPHFQLHTTHSWDFLKYQTSVKVDSGPPSSASDGXYDSIVGILDTGIW 149
Query: 386 PESDSFNDKDMGSIPSGWKGTCMEGNDFKAFKCNRKLIGARYYKETINDEDDRPSKHLND 565
PES+SFNDKDMG IPS WKGTCME DFK+ CNRK+IGARYYK N +DD
Sbjct: 150 PESESFNDKDMGPIPSRWKGTCMEAKDFKSSNCNRKIIGARYYK---NPDDD-------S 199
Query: 566 EGNTARDSQGHGTHTASTAAGSYVSNASYYGLAAGTAKGGSPGSRLAIYKVCASGGCRGS 745
E T RD GHG+H +ST AGS V NASYYG+A+GTAKGGS +R+A+YKVC GGC GS
Sbjct: 200 EYYTTRDVIGHGSHVSSTIAGSAVENASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGS 259
Query: 746 AILAAFDDAISDGVNVISLSLGAAAYMRPNYSNDPIAIGAFHAVEKGITVVCSAGNDGPT 925
+ILAAFDDAI+DGV+V+SLSLGA AY R + + DPIAIGAFHAVE+GI V+CSAGNDGP
Sbjct: 260 SILAAFDDAIADGVDVLSLSLGAPAYARIDLNTDPIAIGAFHAVEQGILVICSAGNDGPD 319
Query: 926 SASVVNAAPWILTVAATTIDRDFESDVIYGENNVIKGGGINFSNLKRSPAHPLIYGKSAK 1105
+V N APWI+TVAA TIDRDFESDV+ G N VIKG GI+FSN+ +SP +PLI+GKSAK
Sbjct: 320 GGTVTNTAPWIMTVAANTIDRDFESDVVLGGNKVIKGEGIHFSNVSKSPVYPLIHGKSAK 379
Query: 1106 TSSSTDNQASNCDPNALDGNKIKGNIVLCHHSDGSYSKREIRDGVEDLEAIGLVLINDLE 1285
++ +++ A CD ++LD K+KG IVLC + GSY RD V+ G V ++D
Sbjct: 380 SADASEGSARACDSDSLDQEKVKGKIVLCENVGGSYYASSARDKVKSKGGTGCVFVDDRT 439
Query: 1286 RSVPSPSGNFPMTIISSKEAAALLSYINSTKNPVATISPTVTVTKYKPAPQVAYFSSRGP 1465
R+V S G+FP T+I SKEAA + SY+NSTK+PVATI PT TV K+ PAP VAYFSSRGP
Sbjct: 440 RAVASAYGSFPTTVIDSKEAAEIFSYLNSTKDPVATILPTATVEKFTPAPAVAYFSSRGP 499
Query: 1466 SDQTKNLLK 1492
S T+++LK
Sbjct: 500 SSLTRSILK 508
>dbj|BAJ53097.1| JHL20J20.3 [Jatropha curcas]
Length = 756
Score = 558 bits (1437), Expect = e-156
Identities = 285/493 (57%), Positives = 361/493 (73%)
Frame = +2
Query: 14 EKKSVYIVYTGAATSRLSSSRDNHIQILSSVLKRNDDAEKRLVHTYSRGFSGCAVWLTEE 193
+ VYIVY GAA + + +++Q+LSS+L R ++ LV +Y GFSG A L+E
Sbjct: 25 QNDGVYIVYMGAANGYVEN---DYVQLLSSILTRKKNS---LVRSYRNGFSGFAARLSEA 78
Query: 194 EARLIGQRSDVVSVFVDPIFKLHTTRSWDFLQYDLETYSTPTLNSNALSDGYDTIIGVFD 373
E + I +R VVSVF DP+ +LHTTRSWDFL+Y + ++S+++S G DTI+G+ D
Sbjct: 79 EVQSIAKRPGVVSVFPDPVLQLHTTRSWDFLKYQTDI----EIDSSSMSHGSDTIVGIID 134
Query: 374 TGIWPESDSFNDKDMGSIPSGWKGTCMEGNDFKAFKCNRKLIGARYYKETINDEDDRPSK 553
TGIWPES+SFNDKDMG IPS WKGTC++G +FK+ CN+K+IGAR+Y +DED+
Sbjct: 135 TGIWPESESFNDKDMGPIPSHWKGTCVKGYNFKSSNCNKKIIGARFYDSPEDDEDEIYQ- 193
Query: 554 HLNDEGNTARDSQGHGTHTASTAAGSYVSNASYYGLAAGTAKGGSPGSRLAIYKVCASGG 733
T RD+ GHGTH A+TAAG+ VSNASYYGLA GTAKGGSP SR+A+Y+VC+ G
Sbjct: 194 -------TPRDAIGHGTHVAATAAGAVVSNASYYGLAEGTAKGGSPMSRIAVYRVCSENG 246
Query: 734 CRGSAILAAFDDAISDGVNVISLSLGAAAYMRPNYSNDPIAIGAFHAVEKGITVVCSAGN 913
C GS ILAAFDDAI+DGV+V+S+SLG + + + D IAIGAFHAVE GITVVCSAGN
Sbjct: 247 CYGSNILAAFDDAIADGVDVLSISLGTPSGFVSDLNKDTIAIGAFHAVENGITVVCSAGN 306
Query: 914 DGPTSASVVNAAPWILTVAATTIDRDFESDVIYGENNVIKGGGINFSNLKRSPAHPLIYG 1093
DGPTS +VVN APWILTVAATTIDRDFESDV+ G N VIKG GINF+++ +SP HPLIYG
Sbjct: 307 DGPTSGTVVNDAPWILTVAATTIDRDFESDVVLGGNKVIKGEGINFADIGKSPVHPLIYG 366
Query: 1094 KSAKTSSSTDNQASNCDPNALDGNKIKGNIVLCHHSDGSYSKREIRDGVEDLEAIGLVLI 1273
KSAKT +T+ A NC ++ IKG IV C++ D + E++ V+ LE IGLVL
Sbjct: 367 KSAKTDVATEMDARNCRSGSMKKEMIKGKIVFCYNDDFEFPGDEMKQEVQSLEGIGLVLA 426
Query: 1274 NDLERSVPSPSGNFPMTIISSKEAAALLSYINSTKNPVATISPTVTVTKYKPAPQVAYFS 1453
+D R+V FPMT+I+S++AA + SYINST+NPVATI PT TV YKPAP VAYFS
Sbjct: 427 DDKTRAVAFNYKEFPMTVINSRDAAEIESYINSTRNPVATILPTTTVINYKPAPTVAYFS 486
Query: 1454 SRGPSDQTKNLLK 1492
SRGPS ++N+LK
Sbjct: 487 SRGPSAISRNILK 499