BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis25_contig00000250
         (1516 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAE97401.1| mitogen-activated protein kinase 2 [Nicotiana ta...   484   e-134
gb|ADT91695.1| mitogen-activated protein kinase kinase 2 [Nicoti...   483   e-134
dbj|BAG31944.1| MAP kinase kinase [Nicotiana benthamiana]             481   e-133
ref|XP_002310949.1| predicted protein [Populus trichocarpa] gi|2...   478   e-132
dbj|BAC81698.1| mitogen-activated protein kinase kinase [Solanum...   477   e-132

>dbj|BAE97401.1| mitogen-activated protein kinase 2 [Nicotiana tabacum]
          Length = 373

 Score =  484 bits (1246), Expect = e-134
 Identities = 248/363 (68%), Positives = 278/363 (76%), Gaps = 17/363 (4%)
 Frame = +2

Query: 290  MRPIQPPKP----TTST-------------NRQRRRPDLTLPLPERIPSLAVXXXXXXXX 418
            MRP+QPP P    TTS+             NR RRR DLTLPLP+R P+LAV        
Sbjct: 1    MRPLQPPPPAAAATTSSSTTASPMPPPPSRNRPRRRTDLTLPLPQRDPALAVPLPLPPTS 60

Query: 419  XXXXXXXXXXXXXXXXXXXXXXXXGALSLTELDKERRIGSGSGGTVYKVRHRPTGRSYAL 598
                                      L+ +EL++  RIGSG+GGTVYKV HRPTGR YAL
Sbjct: 61   APSSSSSSSSSPLPTP----------LNFSELERINRIGSGAGGTVYKVLHRPTGRLYAL 110

Query: 599  KVIYGNHEDAVRLQICREIEILRTVDNPNVVKCHGMFDNAGEIQVLLEHMDCGSLEGNHI 778
            KVIYGNHED+VRLQ+CREIEILR VDNPNVV+CH MFD+ GEIQVLLE MD GSLEG HI
Sbjct: 111  KVIYGNHEDSVRLQMCREIEILRDVDNPNVVRCHDMFDHNGEIQVLLEFMDKGSLEGIHI 170

Query: 779  SQEQALADLSYQILSGLSYLHRRKIVHRDIKPSNLLINSRKQVKIADFGVSRILAQTMDP 958
             +E AL+DL+ Q+LSGL YLHRRKIVHRDIKPSNLLINSR++VKIADFGVSR+LAQTMDP
Sbjct: 171  PKESALSDLTRQVLSGLYYLHRRKIVHRDIKPSNLLINSRREVKIADFGVSRVLAQTMDP 230

Query: 959  CNSSVGTIAYMSPERINTDLNHGVYDGYAGDIWSLGVSILEFYLGKFPFAVGRQGDWASL 1138
            CNSSVGTIAYMSPERINTDLNHG YDGYAGDIWSLGVSILEFYLG+FPF+VGR GDWASL
Sbjct: 231  CNSSVGTIAYMSPERINTDLNHGQYDGYAGDIWSLGVSILEFYLGRFPFSVGRSGDWASL 290

Query: 1139 MCAICISAPPEAPINASREFKNFIACCLQRDPAKRWTAAQLLQHPFIVMNSSPNLNRRHL 1318
            MCAIC+S PPEAP NASREF++FIACCLQRDPA+RWTA QLL+HPFI  NS       ++
Sbjct: 291  MCAICMSQPPEAPANASREFRDFIACCLQRDPARRWTAVQLLRHPFITQNSPAATTTGNM 350

Query: 1319 QPV 1327
             P+
Sbjct: 351  MPL 353


>gb|ADT91695.1| mitogen-activated protein kinase kinase 2 [Nicotiana attenuata]
          Length = 375

 Score =  483 bits (1242), Expect = e-134
 Identities = 247/352 (70%), Positives = 274/352 (77%), Gaps = 18/352 (5%)
 Frame = +2

Query: 290  MRPIQPPKP-----TTST-------------NRQRRRPDLTLPLPERIPSLAVXXXXXXX 415
            MRP+QPP P     TTS+             NR RRR DLTLPLP+R P+LAV       
Sbjct: 1    MRPLQPPPPAAAATTTSSSTTASPMPPPPSRNRPRRRTDLTLPLPQRDPALAVPLPLPPT 60

Query: 416  XXXXXXXXXXXXXXXXXXXXXXXXXGALSLTELDKERRIGSGSGGTVYKVRHRPTGRSYA 595
                                       L+ +EL++  RIGSG+GGTVYKV HRPTGR YA
Sbjct: 61   SAPSSSSSSSSSPLPTP----------LNFSELERINRIGSGAGGTVYKVLHRPTGRLYA 110

Query: 596  LKVIYGNHEDAVRLQICREIEILRTVDNPNVVKCHGMFDNAGEIQVLLEHMDCGSLEGNH 775
            LKVIYGNHED+VRLQ+CREIEILR VDNPNVV+CH MFD+ GEIQVLLE MD GSLEG H
Sbjct: 111  LKVIYGNHEDSVRLQMCREIEILRDVDNPNVVRCHDMFDHNGEIQVLLEFMDKGSLEGIH 170

Query: 776  ISQEQALADLSYQILSGLSYLHRRKIVHRDIKPSNLLINSRKQVKIADFGVSRILAQTMD 955
            I +E AL+DL+ Q+LSGL YLHRRKIVHRDIKPSNLLINSR++VKIADFGVSR+LAQTMD
Sbjct: 171  IPKESALSDLTRQVLSGLYYLHRRKIVHRDIKPSNLLINSRREVKIADFGVSRVLAQTMD 230

Query: 956  PCNSSVGTIAYMSPERINTDLNHGVYDGYAGDIWSLGVSILEFYLGKFPFAVGRQGDWAS 1135
            PCNSSVGTIAYMSPERINTDLNHG YDGYAGDIWSLGVSILEFYLG+FPF+VGR GDWAS
Sbjct: 231  PCNSSVGTIAYMSPERINTDLNHGQYDGYAGDIWSLGVSILEFYLGRFPFSVGRSGDWAS 290

Query: 1136 LMCAICISAPPEAPINASREFKNFIACCLQRDPAKRWTAAQLLQHPFIVMNS 1291
            LMCAIC+S PPEAP NASREF++FIACCLQRDPA+RWTA QLL+HPFI  NS
Sbjct: 291  LMCAICMSQPPEAPANASREFRDFIACCLQRDPARRWTAVQLLRHPFITQNS 342


>dbj|BAG31944.1| MAP kinase kinase [Nicotiana benthamiana]
          Length = 372

 Score =  481 bits (1239), Expect = e-133
 Identities = 244/363 (67%), Positives = 275/363 (75%), Gaps = 17/363 (4%)
 Frame = +2

Query: 290  MRPIQPPKPTTST-----------------NRQRRRPDLTLPLPERIPSLAVXXXXXXXX 418
            MRP+QPP P  +T                 NR RRR DLTLPLP+R P+LAV        
Sbjct: 1    MRPLQPPPPAAATTTSSSTTASPMPPPPSRNRPRRRTDLTLPLPQRDPALAVPLPLPPTS 60

Query: 419  XXXXXXXXXXXXXXXXXXXXXXXXGALSLTELDKERRIGSGSGGTVYKVRHRPTGRSYAL 598
                                      L+ +EL++  RIGSG+GGTVYKV +RPTGR YAL
Sbjct: 61   APSSSSSSSSSPLPTP----------LNFSELERINRIGSGAGGTVYKVLYRPTGRLYAL 110

Query: 599  KVIYGNHEDAVRLQICREIEILRTVDNPNVVKCHGMFDNAGEIQVLLEHMDCGSLEGNHI 778
            KVIYGNHED+VRLQ+CREIEILR VDNPNVV+CH MFD+ GEIQVLLE MD GSLEG HI
Sbjct: 111  KVIYGNHEDSVRLQMCREIEILRDVDNPNVVRCHDMFDHNGEIQVLLEFMDKGSLEGIHI 170

Query: 779  SQEQALADLSYQILSGLSYLHRRKIVHRDIKPSNLLINSRKQVKIADFGVSRILAQTMDP 958
             +E AL+DL+ Q+LSGL YLHRRKIVHRDIKPSNLLINSR++VKIADFGVSR+LAQTMDP
Sbjct: 171  PKESALSDLTRQVLSGLYYLHRRKIVHRDIKPSNLLINSRREVKIADFGVSRVLAQTMDP 230

Query: 959  CNSSVGTIAYMSPERINTDLNHGVYDGYAGDIWSLGVSILEFYLGKFPFAVGRQGDWASL 1138
            CNSSVGTIAYMSPERINTDLNHG YDGYAGDIWSLGVSILEFYLG+FPF+VGR GDWASL
Sbjct: 231  CNSSVGTIAYMSPERINTDLNHGQYDGYAGDIWSLGVSILEFYLGRFPFSVGRSGDWASL 290

Query: 1139 MCAICISAPPEAPINASREFKNFIACCLQRDPAKRWTAAQLLQHPFIVMNSSPNLNRRHL 1318
            MCAIC+S PPEAP NASREF++FIACCLQRDPA+RWTA QLL+HPFI  N        ++
Sbjct: 291  MCAICMSQPPEAPANASREFRDFIACCLQRDPARRWTAVQLLRHPFITQNRPATTTTGNM 350

Query: 1319 QPV 1327
             P+
Sbjct: 351  MPL 353


>ref|XP_002310949.1| predicted protein [Populus trichocarpa] gi|222850769|gb|EEE88316.1|
            predicted protein [Populus trichocarpa]
          Length = 356

 Score =  478 bits (1231), Expect = e-132
 Identities = 242/339 (71%), Positives = 270/339 (79%), Gaps = 8/339 (2%)
 Frame = +2

Query: 290  MRPIQPPKPT--------TSTNRQRRRPDLTLPLPERIPSLAVXXXXXXXXXXXXXXXXX 445
            M+PIQPP P         T+ +R RRRPDLTLPLP+R P+LAV                 
Sbjct: 1    MKPIQPPPPQSPSSTNRHTNNSRPRRRPDLTLPLPQRDPALAVPLPLPPNSVGSNPSSSS 60

Query: 446  XXXXXXXXXXXXXXXGALSLTELDKERRIGSGSGGTVYKVRHRPTGRSYALKVIYGNHED 625
                             LS +ELD+  RIGSGSGGTVYKV HRPTGR YALKVIYGNHED
Sbjct: 61   VSSAR----------AQLSFSELDRINRIGSGSGGTVYKVVHRPTGRFYALKVIYGNHED 110

Query: 626  AVRLQICREIEILRTVDNPNVVKCHGMFDNAGEIQVLLEHMDCGSLEGNHISQEQALADL 805
            +VR QICREIEILR V++PNVVKCH MFD+ GEIQVLLE MD GSLEG HI+ E  L+D+
Sbjct: 111  SVRNQICREIEILRDVNHPNVVKCHDMFDHNGEIQVLLEFMDNGSLEGTHINHEAYLSDV 170

Query: 806  SYQILSGLSYLHRRKIVHRDIKPSNLLINSRKQVKIADFGVSRILAQTMDPCNSSVGTIA 985
            + QIL+G++YLH+RKIVHRDIKPSNLLINS+K VKIADFGVSRILAQTMDPCNSSVGTIA
Sbjct: 171  ARQILNGIAYLHKRKIVHRDIKPSNLLINSKKNVKIADFGVSRILAQTMDPCNSSVGTIA 230

Query: 986  YMSPERINTDLNHGVYDGYAGDIWSLGVSILEFYLGKFPFAVGRQGDWASLMCAICISAP 1165
            YMSPERINTDLN G+YDGYAGDIWSLGVSILEFYLG+FPF VGRQGDWASLMCAIC+S P
Sbjct: 231  YMSPERINTDLNQGLYDGYAGDIWSLGVSILEFYLGRFPFGVGRQGDWASLMCAICMSQP 290

Query: 1166 PEAPINASREFKNFIACCLQRDPAKRWTAAQLLQHPFIV 1282
            PEAP +ASREF+NFIACCLQR+P++RWTA QLLQHPFIV
Sbjct: 291  PEAPASASREFRNFIACCLQREPSRRWTANQLLQHPFIV 329


>dbj|BAC81698.1| mitogen-activated protein kinase kinase [Solanum tuberosum]
          Length = 374

 Score =  477 bits (1227), Expect = e-132
 Identities = 248/369 (67%), Positives = 274/369 (74%), Gaps = 18/369 (4%)
 Frame = +2

Query: 290  MRPIQPPKPTTST------------------NRQRRRPDLTLPLPERIPSLAVXXXXXXX 415
            MRP+QPP P  ++                  +R RRR DLTLPLP+R  +LAV       
Sbjct: 1    MRPLQPPPPAANSTSSAAASSMPPPSSAGQRSRPRRRTDLTLPLPQRDVALAVPLPLPPT 60

Query: 416  XXXXXXXXXXXXXXXXXXXXXXXXXGALSLTELDKERRIGSGSGGTVYKVRHRPTGRSYA 595
                                       L  +EL++  RIGSG+GGTVYKV HRPTGR YA
Sbjct: 61   SAPSSSSSSSSSPLPTP----------LHFSELERVNRIGSGTGGTVYKVLHRPTGRLYA 110

Query: 596  LKVIYGNHEDAVRLQICREIEILRTVDNPNVVKCHGMFDNAGEIQVLLEHMDCGSLEGNH 775
            LKVIYGNHED+VRLQ+CREIEILR VDNPNVV+CH MFD+ GEIQVLLE MD GSLEG H
Sbjct: 111  LKVIYGNHEDSVRLQMCREIEILRDVDNPNVVRCHDMFDHNGEIQVLLEFMDKGSLEGIH 170

Query: 776  ISQEQALADLSYQILSGLSYLHRRKIVHRDIKPSNLLINSRKQVKIADFGVSRILAQTMD 955
            I  EQ L+DL+ Q+LSGL YLHRRKIVHRDIKPSNLLINSR++VKIADFGVSR+LAQTMD
Sbjct: 171  IPLEQPLSDLTRQVLSGLYYLHRRKIVHRDIKPSNLLINSRREVKIADFGVSRVLAQTMD 230

Query: 956  PCNSSVGTIAYMSPERINTDLNHGVYDGYAGDIWSLGVSILEFYLGKFPFAVGRQGDWAS 1135
            PCNSSVGTIAYMSPERINTDLNHG YDGYAGDIWSLGVSILEFYLG+FPF+VGRQGDWAS
Sbjct: 231  PCNSSVGTIAYMSPERINTDLNHGQYDGYAGDIWSLGVSILEFYLGRFPFSVGRQGDWAS 290

Query: 1136 LMCAICISAPPEAPINASREFKNFIACCLQRDPAKRWTAAQLLQHPFIVMNSSPNLNRRH 1315
            LMCAIC+S PPEAP  ASREF+ FIACCLQRDPA+RWTAAQLL+HPFI  NS       H
Sbjct: 291  LMCAICMSQPPEAPPTASREFREFIACCLQRDPARRWTAAQLLRHPFITQNSPGT----H 346

Query: 1316 LQPVNMSLS 1342
              P   SLS
Sbjct: 347  TGPATTSLS 355