BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00035323
         (492 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI16383.3| unnamed protein product [Vitis vinifera]              205   3e-51
ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnm...   205   3e-51
ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase Mnm...   191   4e-47
ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|2...   179   3e-43
ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-m...   176   1e-42

>emb|CBI16383.3| unnamed protein product [Vitis vinifera]
          Length = 544

 Score =  205 bits (522), Expect = 3e-51
 Identities = 101/171 (59%), Positives = 129/171 (75%), Gaps = 7/171 (4%)
 Frame = +1

Query: 1   RKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQG 180
           R FR+GSLKW+S  PP   +QL CKVRHGP FY C+ TMEP +DG +DV +VQL EDDQG
Sbjct: 369 RLFRVGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQG 428

Query: 181 LAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE- 357
           LAAGQF AFY+ ++C+GSG+ILESW+DQGFPVC +ALEIAR+EDKSKLGKPVKI V+PE 
Sbjct: 429 LAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPET 488

Query: 358 -PKGN-----IQLHKEAAMAKITPSEPVIETPRPASQGIFSSCFPMNWLKE 492
            PKG+     I+L +E A ++   ++ +      +S+G   S FPMNWL++
Sbjct: 489 PPKGSDQIDGIKLDRELANSQAAVTDEISR----SSEGEARSRFPMNWLQK 535


>ref|XP_002282827.1| PREDICTED: tRNA-specific 2-thiouridylase mnmA-like [Vitis vinifera]
          Length = 540

 Score =  205 bits (522), Expect = 3e-51
 Identities = 101/171 (59%), Positives = 129/171 (75%), Gaps = 7/171 (4%)
 Frame = +1

Query: 1   RKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQG 180
           R FR+GSLKW+S  PP   +QL CKVRHGP FY C+ TMEP +DG +DV +VQL EDDQG
Sbjct: 365 RLFRVGSLKWISREPPGQINQLQCKVRHGPGFYNCSFTMEPGEDGHEDVAVVQLSEDDQG 424

Query: 181 LAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE- 357
           LAAGQF AFY+ ++C+GSG+ILESW+DQGFPVC +ALEIAR+EDKSKLGKPVKI V+PE 
Sbjct: 425 LAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPVKIKVRPET 484

Query: 358 -PKGN-----IQLHKEAAMAKITPSEPVIETPRPASQGIFSSCFPMNWLKE 492
            PKG+     I+L +E A ++   ++ +      +S+G   S FPMNWL++
Sbjct: 485 PPKGSDQIDGIKLDRELANSQAAVTDEISR----SSEGEARSRFPMNWLQK 531


>ref|XP_004142116.1| PREDICTED: tRNA-specific 2-thiouridylase MnmA-like [Cucumis
           sativus] gi|449524848|ref|XP_004169433.1| PREDICTED:
           tRNA-specific 2-thiouridylase MnmA-like [Cucumis
           sativus]
          Length = 538

 Score =  191 bits (486), Expect = 4e-47
 Identities = 95/169 (56%), Positives = 119/169 (70%), Gaps = 7/169 (4%)
 Frame = +1

Query: 1   RKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQG 180
           R FR+GSLKWLSG PPD F +L CKVRHGPSFY C LTME  +DG +DV +V L EDDQG
Sbjct: 363 RVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQG 422

Query: 181 LAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE- 357
           LAAGQFAAFY+ ++C+GSG+ILESW+DQGFPVC +A+E A++EDKSKLGKP+KI V+PE 
Sbjct: 423 LAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPES 482

Query: 358 ------PKGNIQLHKEAAMAKITPSEPVIETPRPASQGIFSSCFPMNWL 486
                  K + ++H +    + T    V+E  R  S     +   M WL
Sbjct: 483 GMKESKAKDDTEIHDKLRNEEAT----VVEQIREVSPDEAMATPSMKWL 527


>ref|XP_002298404.1| predicted protein [Populus trichocarpa] gi|222845662|gb|EEE83209.1|
           predicted protein [Populus trichocarpa]
          Length = 434

 Score =  179 bits (453), Expect = 3e-43
 Identities = 89/151 (58%), Positives = 110/151 (72%)
 Frame = +1

Query: 1   RKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQG 180
           R FR+GSLKWLSGSP D   QL CKVRHGP+FY C++ +E  +D  ++V  V L EDDQG
Sbjct: 289 RSFRVGSLKWLSGSPTDMTTQLQCKVRHGPTFYDCSVKIELGEDSPEEVVAVHLCEDDQG 348

Query: 181 LAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPEP 360
           LAAGQF AFY+ + C+GSG+ILESW+D+GFPVC +ALE+ARLEDKSKLGKPVKI V+PE 
Sbjct: 349 LAAGQFTAFYQGRTCIGSGVILESWDDKGFPVCQKALELARLEDKSKLGKPVKIKVKPET 408

Query: 361 KGNIQLHKEAAMAKITPSEPVIETPRPASQG 453
                + K+     I P + + E PR A  G
Sbjct: 409 SDQESVQKDG----IEPDKGLAE-PRVAVTG 434


>ref|XP_002513510.1| tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
           putative [Ricinus communis] gi|223547418|gb|EEF48913.1|
           tRNA
           (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
           putative [Ricinus communis]
          Length = 497

 Score =  176 bits (447), Expect = 1e-42
 Identities = 79/119 (66%), Positives = 97/119 (81%)
 Frame = +1

Query: 1   RKFRIGSLKWLSGSPPDHFDQLHCKVRHGPSFYPCTLTMEPVDDGKKDVGLVQLFEDDQG 180
           R  R+GSL+W +GSPP    +L CKVRHGP FY C++ +EP +   +DV +VQL EDDQG
Sbjct: 352 RLLRVGSLRWFNGSPPKETSELQCKVRHGPGFYNCSVVIEPGEVDDQDVAIVQLCEDDQG 411

Query: 181 LAAGQFAAFYRDKNCLGSGIILESWEDQGFPVCTRALEIARLEDKSKLGKPVKIMVQPE 357
           LAAGQFAAFY+ + C+GSG+ILESW DQGFPVC RALE+AR+EDKS+LGKPVKI V+PE
Sbjct: 412 LAAGQFAAFYKGRTCIGSGVILESWNDQGFPVCERALELARIEDKSELGKPVKIKVKPE 470