BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00030152
         (658 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI29911.3| unnamed protein product [Vitis vinifera]              241   7e-62
ref|XP_002277107.1| PREDICTED: cytochrome P450 93A1 [Vitis vinif...   241   7e-62
ref|XP_002323294.1| cytochrome P450 [Populus trichocarpa] gi|222...   234   1e-59
gb|AFG31000.1| flavone synthase II [Cynara cardunculus var. scol...   225   5e-57
ref|XP_002334837.1| cytochrome P450 probable flavone synthase [P...   225   5e-57

>emb|CBI29911.3| unnamed protein product [Vitis vinifera]
          Length = 438

 Score =  241 bits (616), Expect = 7e-62
 Identities = 118/221 (53%), Positives = 159/221 (71%), Gaps = 3/221 (1%)
 Frame = +3

Query: 3   EDEWGKGIRSVVREATEIIATFNLSDFIWFLRHIDVQGIKKRAMNIQFRFDTLLEKIIKE 182
           E+E G+ ++ +V+E  E+   FNLSDFIWF +++D+QG  KR   +  RFD+++EKI++E
Sbjct: 181 ENEAGE-VKRLVQEVAELTGKFNLSDFIWFCKNLDLQGFGKRLKEVHERFDSMIEKIMRE 239

Query: 183 REQVRRKKVE---KSSKXXXXXXXXXXXXXXXKIKLTRANIKAFMFDFLTAATDTSGIVV 353
            E+ R+K++     ++K               +IKLTR NIKAF+ D   A TDTS I  
Sbjct: 240 HEEARKKEMGGGGDAAKDVLDILLDISEDQSSEIKLTRENIKAFILDIFAAGTDTSAITT 299

Query: 354 EWAMSELVNNPALMEKAKEEIDSVVGKNRLVKESDIANLPYLQAIFKETLRLHPPIPIFE 533
           EWA++EL+NNP +MEKA++EIDSVVGKN+LV+ESDIANLPYLQAI KETLRLHP  P+  
Sbjct: 300 EWALAELINNPNIMEKARQEIDSVVGKNKLVEESDIANLPYLQAIVKETLRLHPTGPLIV 359

Query: 534 RESTEDCKVAGYDIPAKSILFINNWSICRDPKTWKNPFKFQ 656
           RES+EDC + GYDIPA + LF+N W+I RDP  W+NP +FQ
Sbjct: 360 RESSEDCTIGGYDIPAGTRLFVNVWAIGRDPNHWENPLEFQ 400


>ref|XP_002277107.1| PREDICTED: cytochrome P450 93A1 [Vitis vinifera]
          Length = 512

 Score =  241 bits (616), Expect = 7e-62
 Identities = 118/221 (53%), Positives = 159/221 (71%), Gaps = 3/221 (1%)
 Frame = +3

Query: 3   EDEWGKGIRSVVREATEIIATFNLSDFIWFLRHIDVQGIKKRAMNIQFRFDTLLEKIIKE 182
           E+E G+ ++ +V+E  E+   FNLSDFIWF +++D+QG  KR   +  RFD+++EKI++E
Sbjct: 198 ENEAGE-VKRLVQEVAELTGKFNLSDFIWFCKNLDLQGFGKRLKEVHERFDSMIEKIMRE 256

Query: 183 REQVRRKKVE---KSSKXXXXXXXXXXXXXXXKIKLTRANIKAFMFDFLTAATDTSGIVV 353
            E+ R+K++     ++K               +IKLTR NIKAF+ D   A TDTS I  
Sbjct: 257 HEEARKKEMGGGGDAAKDVLDILLDISEDQSSEIKLTRENIKAFILDIFAAGTDTSAITT 316

Query: 354 EWAMSELVNNPALMEKAKEEIDSVVGKNRLVKESDIANLPYLQAIFKETLRLHPPIPIFE 533
           EWA++EL+NNP +MEKA++EIDSVVGKN+LV+ESDIANLPYLQAI KETLRLHP  P+  
Sbjct: 317 EWALAELINNPNIMEKARQEIDSVVGKNKLVEESDIANLPYLQAIVKETLRLHPTGPLIV 376

Query: 534 RESTEDCKVAGYDIPAKSILFINNWSICRDPKTWKNPFKFQ 656
           RES+EDC + GYDIPA + LF+N W+I RDP  W+NP +FQ
Sbjct: 377 RESSEDCTIGGYDIPAGTRLFVNVWAIGRDPNHWENPLEFQ 417


>ref|XP_002323294.1| cytochrome P450 [Populus trichocarpa] gi|222867924|gb|EEF05055.1|
           cytochrome P450 [Populus trichocarpa]
          Length = 511

 Score =  234 bits (596), Expect = 1e-59
 Identities = 112/221 (50%), Positives = 154/221 (69%), Gaps = 4/221 (1%)
 Frame = +3

Query: 6   DEWGKGIRSVVREATEIIATFNLSDFIWFLRHIDVQGIKKRAMNIQFRFDTLLEKIIKER 185
           D+    +R++V E  ++   FNLSDFIWF +++D+QG  KR   ++ RFDT+ E+II E 
Sbjct: 198 DDEADEVRNLVHEVADLTGKFNLSDFIWFCKNLDLQGFGKRLKEVRKRFDTMTERIIMEH 257

Query: 186 EQVRRKKVEKSS----KXXXXXXXXXXXXXXXKIKLTRANIKAFMFDFLTAATDTSGIVV 353
           E+ R+KK E       K               ++KLTR NIKAF+ D   A TDTS + +
Sbjct: 258 EEARKKKKETGEGDPVKDLLDILLDISEDDSSEMKLTRENIKAFILDIFAAGTDTSAVTM 317

Query: 354 EWAMSELVNNPALMEKAKEEIDSVVGKNRLVKESDIANLPYLQAIFKETLRLHPPIPIFE 533
           EWA++EL+NNP ++E+A+EEIDSVVG++RLV+ESDIANLPY+QAI KETLRLHP  PI  
Sbjct: 318 EWALAELINNPNILERAREEIDSVVGQSRLVQESDIANLPYVQAILKETLRLHPTGPIIL 377

Query: 534 RESTEDCKVAGYDIPAKSILFINNWSICRDPKTWKNPFKFQ 656
           RES+E C + GY+IPA++ LF+N W+I RDP  W+NP +F+
Sbjct: 378 RESSESCTINGYEIPARTRLFVNVWAINRDPNYWENPLEFE 418


>gb|AFG31000.1| flavone synthase II [Cynara cardunculus var. scolymus]
          Length = 513

 Score =  225 bits (574), Expect = 5e-57
 Identities = 112/213 (52%), Positives = 150/213 (70%), Gaps = 3/213 (1%)
 Frame = +3

Query: 27  RSVVREATEIIATFNLSDFIWFLRHIDVQGIKKRAMNIQFRFDTLLEKIIKEREQVRRKK 206
           +++VRE T+I   FN+SDFIWF ++ID+QG KKR  +   R+D LLEKII ERE+ RRK+
Sbjct: 204 KNLVREVTQIFGEFNVSDFIWFCKNIDLQGFKKRYTDTHKRYDALLEKIIFEREEKRRKE 263

Query: 207 VEKSS---KXXXXXXXXXXXXXXXKIKLTRANIKAFMFDFLTAATDTSGIVVEWAMSELV 377
            ++     K               +IK+TR +IKA + DF TAATDT+ I +EW + EL+
Sbjct: 264 GKREDGKGKDFLDMLLDVLEDAKAEIKITRDHIKALILDFFTAATDTTAIALEWMLVELI 323

Query: 378 NNPALMEKAKEEIDSVVGKNRLVKESDIANLPYLQAIFKETLRLHPPIPIFERESTEDCK 557
           +NP ++E A+EEID VVG  RLV+ESD  NLPY+QAI KE LRLHPPIP+  R+S ED  
Sbjct: 324 SNPKVLEIAREEIDQVVGNERLVQESDAPNLPYIQAIIKEALRLHPPIPMLIRKSIEDVS 383

Query: 558 VAGYDIPAKSILFINNWSICRDPKTWKNPFKFQ 656
           V GYDIPA ++LF+N WSI R+PK W++P +F+
Sbjct: 384 VQGYDIPAGTMLFVNIWSIGRNPKYWESPLEFK 416


>ref|XP_002334837.1| cytochrome P450 probable flavone synthase [Populus trichocarpa]
           gi|222875137|gb|EEF12268.1| cytochrome P450 probable
           flavone synthase [Populus trichocarpa]
          Length = 522

 Score =  225 bits (574), Expect = 5e-57
 Identities = 108/218 (49%), Positives = 149/218 (68%), Gaps = 1/218 (0%)
 Frame = +3

Query: 6   DEWGKGIRSVVREATEIIATFNLSDFIWFLRHIDVQGIKKRAMNIQFRFDTLLEKIIKER 185
           D  G+  +++ RE T+I   FN+SDFIW  R+ D QG +K++ ++  RFD LL+ II  R
Sbjct: 192 DSQGEDAKTLAREVTQIFGEFNVSDFIWLCRNFDFQGSRKKSEDVHTRFDALLDNIITNR 251

Query: 186 EQVRRKKVEK-SSKXXXXXXXXXXXXXXXKIKLTRANIKAFMFDFLTAATDTSGIVVEWA 362
           E  R++   K  ++               +I+ TR +IKA + DFLTA TDT+    EWA
Sbjct: 252 ELERKQSGGKVQARDLLDMMLDTLEAQNSEIEFTRDHIKALVLDFLTAGTDTTAASTEWA 311

Query: 363 MSELVNNPALMEKAKEEIDSVVGKNRLVKESDIANLPYLQAIFKETLRLHPPIPIFERES 542
           ++EL+N+P ++EKA++EID+VVG  RLV+ESD  NLPYLQAIFKET RLHPPIP+  R+S
Sbjct: 312 LAELINHPKILEKARQEIDAVVGNKRLVEESDFPNLPYLQAIFKETFRLHPPIPMISRKS 371

Query: 543 TEDCKVAGYDIPAKSILFINNWSICRDPKTWKNPFKFQ 656
           T++CK+ GY IPA S+LF+N WSI RD K W NP +F+
Sbjct: 372 TQECKINGYTIPANSLLFVNMWSIGRDSKYWTNPSEFE 409