BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00029628
         (707 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADN33954.1| 4-coumarate-CoA ligase [Cucumis melo subsp. melo]      224   1e-56
gb|AGE10595.1| 4-coumarate CoA ligase [Lonicera japonica]             214   1e-53
ref|XP_002511221.1| AMP dependent CoA ligase, putative [Ricinus ...   213   3e-53
ref|XP_002315623.1| acyl:coa ligase [Populus trichocarpa] gi|222...   211   2e-52
ref|XP_003602078.1| 4-coumarate-coa ligase [Medicago truncatula]...   209   4e-52

>gb|ADN33954.1| 4-coumarate-CoA ligase [Cucumis melo subsp. melo]
          Length = 1055

 Score =  224 bits (571), Expect = 1e-56
 Identities = 123/231 (53%), Positives = 161/231 (69%), Gaps = 5/231 (2%)
 Frame = +3

Query: 27   YNFKSSMACTSKHD---YNFDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXX 197
            + F S  A  S  +   ++ DL+SGF   T+IFHSLRP + LPP + PLS  QHA     
Sbjct: 482  FEFTSMAARNSNFNAAPHSIDLRSGFCPLTKIFHSLRPPLSLPPLSQPLSVTQHAFSLLQ 541

Query: 198  XXXXXXXXTTALIDSVSGTRVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVP 377
                    +T LIDS SG  +S+  FL+Q  +L+ +LK+  SL  G VAFIL+P  LQ+P
Sbjct: 542  SSSPPPN-STLLIDSNSGLHLSYAIFLRQIRNLASNLKSLTSLCNGHVAFILAPTSLQIP 600

Query: 378  ILYISLLSLGVIVSPANPASTVSEVSRQVGLTNPVIAFAVASTASKIPSLRYGTVFLDSS 557
            +LY +LLSLGV++SPANP S+VSE+S Q+ L+ PVIAFA +STASK+P+LR+GTV +DS 
Sbjct: 601  VLYFALLSLGVVLSPANPTSSVSEISHQIQLSKPVIAFATSSTASKLPTLRFGTVIIDSP 660

Query: 558  EFKSML--VNGVDNRNEVMVNQSDTAAILYSSGTTGRVKGVELTHRNFIAL 704
             F SML   NG D   ++ ++QSD+AAILYSSGTTGRVKGV L+HRN IA+
Sbjct: 661  HFLSMLTETNGSDGLTDIKIDQSDSAAILYSSGTTGRVKGVLLSHRNLIAV 711



 Score =  209 bits (532), Expect = 4e-52
 Identities = 116/221 (52%), Positives = 151/221 (68%), Gaps = 8/221 (3%)
 Frame = +3

Query: 69  YNFDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVS 248
           ++ D +SGF S+T+I+ SLRP + LPP + PL+ A HA+            T ALIDS S
Sbjct: 4   HSVDSRSGFCSQTKIYKSLRPPLSLPPLSQPLTVAGHALSVLRSSPPPPN-TAALIDSDS 62

Query: 249 GTRVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPAN 428
           G  VS+  FL+Q  +L+ +LK  +S   G VAFILSP  LQ+P+LY +LLSLGV+VSPAN
Sbjct: 63  GVSVSYALFLRQIRNLTSNLKALYSFSNGQVAFILSPTSLQIPVLYFALLSLGVVVSPAN 122

Query: 429 PASTVSEVSRQVGLTNPVIAFAVASTASKIPSLRYGTVFLDSSEFKSMLVNGVDNRNE-- 602
           P S+ SE++ QV L  PVIAFA++STASKIP L  GTV +DS EF S++     NR+E  
Sbjct: 123 PISSESEIAHQVSLCKPVIAFAISSTASKIPRLPLGTVLIDSPEFLSLM--NESNRSEGV 180

Query: 603 ------VMVNQSDTAAILYSSGTTGRVKGVELTHRNFIALV 707
                 + +NQ+D+AAILYSSGTTGRVKGV L+HRN I  +
Sbjct: 181 NDGIFDLKINQNDSAAILYSSGTTGRVKGVLLSHRNLITAI 221


>gb|AGE10595.1| 4-coumarate CoA ligase [Lonicera japonica]
          Length = 540

 Score =  214 bits (545), Expect = 1e-53
 Identities = 114/211 (54%), Positives = 152/211 (72%)
 Frame = +3

Query: 75  FDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVSGT 254
           FD  SGFSS+T+ FHSL P + LPP    +S+A +++            +T LIDS +G 
Sbjct: 4   FDPNSGFSSQTKTFHSLTPPLPLPPETALISSATYSLSLQLTSPWPD--STXLIDSATGQ 61

Query: 255 RVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPANPA 434
           R+S+++F  Q  +LS SL+++  L KGDVAFILS N LQ+P+LY SLLSLGVI+SP+NP+
Sbjct: 62  RLSYSQFTTQTNNLSSSLQSHLGLSKGDVAFILSLNSLQIPLLYFSLLSLGVIISPSNPS 121

Query: 435 STVSEVSRQVGLTNPVIAFAVASTASKIPSLRYGTVFLDSSEFKSMLVNGVDNRNEVMVN 614
           ST SE+SRQ+ L+ PVIAFA ++T+ KIP+LR+GTV +DS EF SM  +     + V V+
Sbjct: 122 STESEISRQIKLSKPVIAFATSATSHKIPALRHGTVLIDSPEFVSMTTSSGRELSCVGVS 181

Query: 615 QSDTAAILYSSGTTGRVKGVELTHRNFIALV 707
           Q D AAI+YSSGTTG+VKGV LTHRN  A+V
Sbjct: 182 QCDIAAIMYSSGTTGQVKGVMLTHRNLTAIV 212


>ref|XP_002511221.1| AMP dependent CoA ligase, putative [Ricinus communis]
           gi|223550336|gb|EEF51823.1| AMP dependent CoA ligase,
           putative [Ricinus communis]
          Length = 549

 Score =  213 bits (542), Expect = 3e-53
 Identities = 117/212 (55%), Positives = 142/212 (66%)
 Frame = +3

Query: 72  NFDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVSG 251
           N D  +GF  +T+ FHSLRP   LPP + PLS AQ+A+            TT LIDS + 
Sbjct: 7   NIDPNNGFCKETKTFHSLRPSTPLPPSDQPLSIAQYAVSLLNSTTANLTATTFLIDSNTA 66

Query: 252 TRVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPANP 431
             ++F++FL +  SLS SLKT+FSL   DVAFIL P  L VP+LY SLLSLG+ +SPANP
Sbjct: 67  ESLTFSQFLNRTRSLSFSLKTHFSLSPNDVAFILCPASLHVPLLYFSLLSLGLTISPANP 126

Query: 432 ASTVSEVSRQVGLTNPVIAFAVASTASKIPSLRYGTVFLDSSEFKSMLVNGVDNRNEVMV 611
            S+ SEV+ Q  L+ P IAFA + TA KIPS   GT+ +DS EF S L N  +    V V
Sbjct: 127 LSSNSEVAHQTQLSQPKIAFATSQTAHKIPSPPSGTILIDSPEFLSFLHNS-NTATYVSV 185

Query: 612 NQSDTAAILYSSGTTGRVKGVELTHRNFIALV 707
           NQSD AAILYSSGTTG+VKGV LTHRN IAL+
Sbjct: 186 NQSDPAAILYSSGTTGKVKGVSLTHRNIIALI 217


>ref|XP_002315623.1| acyl:coa ligase [Populus trichocarpa] gi|222864663|gb|EEF01794.1|
           acyl:coa ligase [Populus trichocarpa]
          Length = 552

 Score =  211 bits (536), Expect = 2e-52
 Identities = 112/214 (52%), Positives = 144/214 (67%), Gaps = 4/214 (1%)
 Frame = +3

Query: 78  DLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTALIDSVSGTR 257
           D KSGF SKT  +HSLRP +QLPP   P+SA ++AI            TTAL+D+V+G R
Sbjct: 10  DPKSGFCSKTNTYHSLRPHLQLPPITTPVSATEYAISLLLYSFPSPPQTTALLDAVTGRR 69

Query: 258 VSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGVIVSPANPAS 437
           +SF E +   E+L+ SL   F LKKGD AFILSPN + +PILY+SL +LGV++SP+NP S
Sbjct: 70  ISFPELIHFTETLASSLLNRFRLKKGDTAFILSPNSIHIPILYLSLFTLGVVISPSNPLS 129

Query: 438 TVSEVSRQVGLTNPVIAFAVASTASKIPSLRYGTVFLDSSEFKSMLVN----GVDNRNEV 605
           +  E+  Q  L+ PVIAF  + TA KIP     T+ LDS EF+S++ +     V+    V
Sbjct: 130 SEQEILHQTNLSKPVIAFVTSQTAHKIPYSVKKTILLDSPEFESLMTSQTQGTVNGLERV 189

Query: 606 MVNQSDTAAILYSSGTTGRVKGVELTHRNFIALV 707
            V QSD AAILYSSGTTGR KGV LTHRNFI+++
Sbjct: 190 RVYQSDPAAILYSSGTTGRFKGVLLTHRNFISML 223


>ref|XP_003602078.1| 4-coumarate-coa ligase [Medicago truncatula]
           gi|355491126|gb|AES72329.1| 4-coumarate-coa ligase
           [Medicago truncatula]
          Length = 560

 Score =  209 bits (532), Expect = 4e-52
 Identities = 116/222 (52%), Positives = 150/222 (67%), Gaps = 4/222 (1%)
 Frame = +3

Query: 54  TSKHDYN-FDLKSGFSSKTRIFHSLRPKVQLPPPNLPLSAAQHAIXXXXXXXXXXXXTTA 230
           T  H  N  DL +G+ S +R FHSLRP   LPPP+ PLS  ++A+            TTA
Sbjct: 9   TQSHSNNTIDLNTGYCSLSRTFHSLRPIAPLPPPSQPLSLTEYALSLLSTTTAVSNNTTA 68

Query: 231 LIDSVSGTRVSFTEFLKQAESLSVSLKTNFSLKKGDVAFILSPNILQVPILYISLLSLGV 410
           LID+ +   +++  FL+Q +SLS  L+++ +L KG VA IL P+ + VP+LY SLLSLGV
Sbjct: 69  LIDATTDHHLTYPTFLRQIKSLSSFLQSHTTLSKGHVALILIPSSIHVPVLYFSLLSLGV 128

Query: 411 IVSPANPASTVSEVSRQVGLTNPVIAFAVASTASKIPSLRYGTVFLDSSEFKSMLVNGVD 590
            ++PANP S+ SE+S  V L NPVIAF+ +STA+KIP L  GTV  DS  F S+L + +D
Sbjct: 129 TIAPANPLSSPSELSHLVHLINPVIAFSTSSTATKIPKLPLGTVIFDSPSFLSVLNSTID 188

Query: 591 NRNE---VMVNQSDTAAILYSSGTTGRVKGVELTHRNFIALV 707
              E   V  +QSDTAAIL+SSGTTGRVKGV LTHRNFIAL+
Sbjct: 189 TDTELRPVETSQSDTAAILFSSGTTGRVKGVLLTHRNFIALI 230