BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00027964
         (1073 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002269753.1| PREDICTED: subtilisin-like protease-like [Vi...   374   e-101
emb|CBI19918.3| unnamed protein product [Vitis vinifera]              367   3e-99
ref|XP_002307740.1| predicted protein [Populus trichocarpa] gi|2...   351   2e-94
ref|NP_564107.1| Subtilisin-like serine endopeptidase-like prote...   342   7e-92
ref|NP_001236511.1| subtilisin-type protease precursor [Glycine ...   342   7e-92

>ref|XP_002269753.1| PREDICTED: subtilisin-like protease-like [Vitis vinifera]
          Length = 768

 Score =  374 bits (960), Expect = e-101
 Identities = 198/311 (63%), Positives = 234/311 (75%), Gaps = 3/311 (0%)
 Frame = +3

Query: 150  ESRADKGEKKSVYIVYTGAATSRLSSSRDNHIQILSSVLKRNDDAEKRLVHTYSRGFSGC 329
            E+RAD+ +   +YIVY GAATS   S R +H QILSS+LKR  +A   LVH+Y  GFSG 
Sbjct: 20   ETRADEMKNDRIYIVYMGAATSSEGSYRYDHAQILSSLLKRKANA---LVHSYRHGFSGF 76

Query: 330  AVWLTEEEARLIGQRSDVVSVFVDPIFKLHTTRSWDFLQY--DLETYSTPTLNSNALSDG 503
            A  LTEEEAR I Q+  VVSVF DP+ +LHTTRSWDFL Y  DLET S P  + ++ S G
Sbjct: 77   AAHLTEEEARSIAQKPGVVSVFEDPVLQLHTTRSWDFLHYQTDLETDSKPGSDGDSQSSG 136

Query: 504  Y-DTIIGVFDTGIWPESDSFNDKDMGSIPSGWKGTCMEGNDFKAFKCNRKLIGARYYKET 680
              DTIIG+ DTGIWPES+SF+DK MG +PS W+GTCME ND  +FKCNRKLIGARYY   
Sbjct: 137  QADTIIGILDTGIWPESESFSDKTMGPVPSRWRGTCMESNDVDSFKCNRKLIGARYY--- 193

Query: 681  INDEDDRPSKHLNDEGNTARDSQGHGTHTASTAAGSYVSNASYYGLAAGTAKGGSPGSRL 860
             ND D   +       +TARD  GHGTH ASTAAG+ + + SYYGLA+GTAKGGSPGSR+
Sbjct: 194  -NDSDAASAVP-----HTARDMIGHGTHVASTAAGNSLPDVSYYGLASGTAKGGSPGSRI 247

Query: 861  AIYKVCASGGCRGSAILAAFDDAISDGVNVISLSLGAAAYMRPNYSNDPIAIGAFHAVEK 1040
            A+Y+VC   GCRGS+ILAAFDDAISDGV+V+SLSLG++A     +S DPIAIGA+HAV K
Sbjct: 248  AMYRVCTFFGCRGSSILAAFDDAISDGVDVLSLSLGSSAVFELEFSTDPIAIGAYHAVAK 307

Query: 1041 GITVVCSAGND 1073
            GITVVCSAGND
Sbjct: 308  GITVVCSAGND 318


>emb|CBI19918.3| unnamed protein product [Vitis vinifera]
          Length = 743

 Score =  367 bits (942), Expect = 3e-99
 Identities = 194/300 (64%), Positives = 227/300 (75%), Gaps = 3/300 (1%)
 Frame = +3

Query: 183  VYIVYTGAATSRLSSSRDNHIQILSSVLKRNDDAEKRLVHTYSRGFSGCAVWLTEEEARL 362
            +YIVY GAATS   S R +H QILSS+LKR  +A   LVH+Y  GFSG A  LTEEEAR 
Sbjct: 6    IYIVYMGAATSSEGSYRYDHAQILSSLLKRKANA---LVHSYRHGFSGFAAHLTEEEARS 62

Query: 363  IGQRSDVVSVFVDPIFKLHTTRSWDFLQY--DLETYSTPTLNSNALSDGY-DTIIGVFDT 533
            I Q+  VVSVF DP+ +LHTTRSWDFL Y  DLET S P  + ++ S G  DTIIG+ DT
Sbjct: 63   IAQKPGVVSVFEDPVLQLHTTRSWDFLHYQTDLETDSKPGSDGDSQSSGQADTIIGILDT 122

Query: 534  GIWPESDSFNDKDMGSIPSGWKGTCMEGNDFKAFKCNRKLIGARYYKETINDEDDRPSKH 713
            GIWPES+SF+DK MG +PS W+GTCME ND  +FKCNRKLIGARYY    ND D   +  
Sbjct: 123  GIWPESESFSDKTMGPVPSRWRGTCMESNDVDSFKCNRKLIGARYY----NDSDAASAVP 178

Query: 714  LNDEGNTARDSQGHGTHTASTAAGSYVSNASYYGLAAGTAKGGSPGSRLAIYKVCASGGC 893
                 +TARD  GHGTH ASTAAG+ + + SYYGLA+GTAKGGSPGSR+A+Y+VC   GC
Sbjct: 179  -----HTARDMIGHGTHVASTAAGNSLPDVSYYGLASGTAKGGSPGSRIAMYRVCTFFGC 233

Query: 894  RGSAILAAFDDAISDGVNVISLSLGAAAYMRPNYSNDPIAIGAFHAVEKGITVVCSAGND 1073
            RGS+ILAAFDDAISDGV+V+SLSLG++A     +S DPIAIGA+HAV KGITVVCSAGND
Sbjct: 234  RGSSILAAFDDAISDGVDVLSLSLGSSAVFELEFSTDPIAIGAYHAVAKGITVVCSAGND 293


>ref|XP_002307740.1| predicted protein [Populus trichocarpa] gi|222857189|gb|EEE94736.1|
            predicted protein [Populus trichocarpa]
          Length = 769

 Score =  351 bits (901), Expect = 2e-94
 Identities = 183/310 (59%), Positives = 221/310 (71%), Gaps = 2/310 (0%)
 Frame = +3

Query: 150  ESRADKGEKKSVYIVYTGAATSRLSSSRDNHIQILSSVLKRNDDAEKRLVHTYSRGFSGC 329
            +S AD+GEK  VYIVY GAAT+   SS++ H Q+LSSVLKR  +A   LVH+Y  G SG 
Sbjct: 21   KSGADEGEKDGVYIVYMGAATAN-GSSKNEHAQLLSSVLKRRKNA---LVHSYEHGISGF 76

Query: 330  AVWLTEEEARLIGQRSDVVSVFVDPIFKLHTTRSWDFLQY--DLETYSTPTLNSNALSDG 503
               L+  EA+ I +   VVSVF DP+++LHTTRSWDFL+Y  D++   +P  +SN  S G
Sbjct: 77   TARLSAAEAQSIAKNPGVVSVFPDPVYQLHTTRSWDFLKYGTDVKIDLSPNSDSNLSSRG 136

Query: 504  YDTIIGVFDTGIWPESDSFNDKDMGSIPSGWKGTCMEGNDFKAFKCNRKLIGARYYKETI 683
            YD IIG+ DTGIWPES SF+DKDM  IPS WKGTC+E  DF +  CNRKLIGAR Y    
Sbjct: 137  YDVIIGILDTGIWPESKSFSDKDMDPIPSSWKGTCVEARDFNSSNCNRKLIGARSYNGPG 196

Query: 684  NDEDDRPSKHLNDEGNTARDSQGHGTHTASTAAGSYVSNASYYGLAAGTAKGGSPGSRLA 863
            +D+D           NT RD  GHGTH ASTAAG  V  ASY+GLA+GTAKGGS GSR+A
Sbjct: 197  DDDDGLV--------NTPRDMNGHGTHVASTAAGIMVPGASYHGLASGTAKGGSLGSRIA 248

Query: 864  IYKVCASGGCRGSAILAAFDDAISDGVNVISLSLGAAAYMRPNYSNDPIAIGAFHAVEKG 1043
            +Y++C   GC GS+ILAAF DAI DGV+++SLSLG+ A    ++  DPIAIGAFHAVE G
Sbjct: 249  VYRICTPNGCAGSSILAAFSDAIKDGVDILSLSLGSPASRISDFKEDPIAIGAFHAVENG 308

Query: 1044 ITVVCSAGND 1073
            ITVVCSAGND
Sbjct: 309  ITVVCSAGND 318


>ref|NP_564107.1| Subtilisin-like serine endopeptidase-like protein [Arabidopsis
            thaliana] gi|8778982|gb|AAF79897.1|AC022472_6 Contains
            similarity to p69c gene from Lycopersicon esculentum
            gb|Y17277 and is a member of subtilase family PF|00082.
            ESTs gb|T22485, gb|R65370, gb|AA651071 come from this
            gene [Arabidopsis thaliana] gi|110737651|dbj|BAF00765.1|
            hypothetical protein [Arabidopsis thaliana]
            gi|332191824|gb|AEE29945.1| Subtilisin-like serine
            endopeptidase-like protein [Arabidopsis thaliana]
          Length = 769

 Score =  342 bits (878), Expect = 7e-92
 Identities = 183/331 (55%), Positives = 224/331 (67%)
 Frame = +3

Query: 81   MKGISVYCYVXXXXXXXXXXXXGESRADKGEKKSVYIVYTGAATSRLSSSRDNHIQILSS 260
            MKGI+ +                E+ A       VYIVY G+A+S  +++R   I I + 
Sbjct: 1    MKGITFFTPFLSFLYLLCILFMTETEAGSRNGDGVYIVYMGSASSAANANRAQ-ILINTM 59

Query: 261  VLKRNDDAEKRLVHTYSRGFSGCAVWLTEEEARLIGQRSDVVSVFVDPIFKLHTTRSWDF 440
              +R +D    L+HTY  GFSG A  LT EEA++I ++  VVSVF DP F+LHTT SWDF
Sbjct: 60   FKRRAND----LLHTYKHGFSGFAARLTAEEAKVIAKKPGVVSVFPDPHFQLHTTHSWDF 115

Query: 441  LQYDLETYSTPTLNSNALSDGYDTIIGVFDTGIWPESDSFNDKDMGSIPSGWKGTCMEGN 620
            L+Y           S+A    YD+I+G+ DTGIWPES+SFNDKDMG IPS WKGTCME  
Sbjct: 116  LKYQTSVKVDSGPPSSASDGSYDSIVGILDTGIWPESESFNDKDMGPIPSRWKGTCMEAK 175

Query: 621  DFKAFKCNRKLIGARYYKETINDEDDRPSKHLNDEGNTARDSQGHGTHTASTAAGSYVSN 800
            DFK+  CNRK+IGARYYK   N +DD        E  T RD  GHG+H +ST AGS V N
Sbjct: 176  DFKSSNCNRKIIGARYYK---NPDDD-------SEYYTTRDVIGHGSHVSSTIAGSAVEN 225

Query: 801  ASYYGLAAGTAKGGSPGSRLAIYKVCASGGCRGSAILAAFDDAISDGVNVISLSLGAAAY 980
            ASYYG+A+GTAKGGS  +R+A+YKVC  GGC GS+ILAAFDDAI+DGV+V+SLSLGA AY
Sbjct: 226  ASYYGVASGTAKGGSQNARIAMYKVCNPGGCTGSSILAAFDDAIADGVDVLSLSLGAPAY 285

Query: 981  MRPNYSNDPIAIGAFHAVEKGITVVCSAGND 1073
             R + + DPIAIGAFHAVE+GI V+CSAGND
Sbjct: 286  ARIDLNTDPIAIGAFHAVEQGILVICSAGND 316


>ref|NP_001236511.1| subtilisin-type protease precursor [Glycine max]
            gi|14150446|gb|AAK53065.1| subtilisin-type protease
            precursor [Glycine max]
          Length = 766

 Score =  342 bits (878), Expect = 7e-92
 Identities = 178/309 (57%), Positives = 223/309 (72%)
 Frame = +3

Query: 147  GESRADKGEKKSVYIVYTGAATSRLSSSRDNHIQILSSVLKRNDDAEKRLVHTYSRGFSG 326
            G    D   +K VYIVY GAA S  +S R++H Q+L+SVL+RN++A   LV  Y  GFSG
Sbjct: 28   GNGSNDVTNRKEVYIVYMGAADSTDASFRNDHAQVLNSVLRRNENA---LVRNYKHGFSG 84

Query: 327  CAVWLTEEEARLIGQRSDVVSVFVDPIFKLHTTRSWDFLQYDLETYSTPTLNSNALSDGY 506
             A  L+++EA  I Q+  VVSVF  P+ KLHTTRSWDFL+Y  +T        NA+S   
Sbjct: 85   FAARLSKKEATSIAQKPGVVSVFPGPVLKLHTTRSWDFLKY--QTQVKIDTKPNAVSKS- 141

Query: 507  DTIIGVFDTGIWPESDSFNDKDMGSIPSGWKGTCMEGNDFKAFKCNRKLIGARYYKETIN 686
             ++IG+ DTGIWPE+ SF+DK MG +PS WKGTCM+  DF +  CNRKLIGARYY +  N
Sbjct: 142  SSVIGILDTGIWPEAASFSDKGMGPVPSRWKGTCMKSQDFYSSNCNRKLIGARYYADP-N 200

Query: 687  DEDDRPSKHLNDEGNTARDSQGHGTHTASTAAGSYVSNASYYGLAAGTAKGGSPGSRLAI 866
            D  D          NTARDS GHGTH A TAAG  V+NASYYG+A G AKGGSP SRLA+
Sbjct: 201  DSGD----------NTARDSNGHGTHVAGTAAGVMVTNASYYGVATGCAKGGSPESRLAV 250

Query: 867  YKVCASGGCRGSAILAAFDDAISDGVNVISLSLGAAAYMRPNYSNDPIAIGAFHAVEKGI 1046
            Y+VC++ GCRGS+ILAAFDDAI+DGV+++S+SLGA+   RP+ ++DPI++GAFHA+E GI
Sbjct: 251  YRVCSNFGCRGSSILAAFDDAIADGVDLLSVSLGASTGFRPDLTSDPISLGAFHAMEHGI 310

Query: 1047 TVVCSAGND 1073
             VVCSAGND
Sbjct: 311  LVVCSAGND 319