BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00022475
         (539 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       116   1e-36
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   116   6e-31
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       110   8e-30
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   110   4e-29
gb|ACO90244.1| canadine synthase [Thalictrum flavum]                   91   2e-23

>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  116 bits (291), Expect(2) = 1e-36
 Identities = 56/59 (94%), Positives = 58/59 (98%)
 Frame = -2

Query: 538 FMPERFLQGEDGSANNKAMEQSFLPFGAGMRICAGMNLGKLQIAFALANLVNAFKWSCV 362
           FMPERFLQGE+ SANNKAMEQSFLPFGAGMRICAGM+LGKLQIAFALANLVNAFKWSCV
Sbjct: 402 FMPERFLQGENVSANNKAMEQSFLPFGAGMRICAGMDLGKLQIAFALANLVNAFKWSCV 460



 Score = 61.6 bits (148), Expect(2) = 1e-36
 Identities = 30/30 (100%), Positives = 30/30 (100%)
 Frame = -3

Query: 258 EGKLPDMSEQLFAVTLMKTPLEGRISTRKV 169
           EGKLPDMSEQLFAVTLMKTPLEGRISTRKV
Sbjct: 462 EGKLPDMSEQLFAVTLMKTPLEGRISTRKV 491


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  116 bits (290), Expect(2) = 6e-31
 Identities = 55/59 (93%), Positives = 57/59 (96%)
 Frame = -2

Query: 538 FMPERFLQGEDGSANNKAMEQSFLPFGAGMRICAGMNLGKLQIAFALANLVNAFKWSCV 362
           FMPERFLQGEDGSA+NKAMEQSFLPF AGMRICAGM+LGKLQ AFALANLVNAFKWSCV
Sbjct: 403 FMPERFLQGEDGSASNKAMEQSFLPFSAGMRICAGMDLGKLQFAFALANLVNAFKWSCV 461



 Score = 43.1 bits (100), Expect(2) = 6e-31
 Identities = 20/29 (68%), Positives = 23/29 (79%)
 Frame = -3

Query: 258 EGKLPDMSEQLFAVTLMKTPLEGRISTRK 172
           EGK PDMSE+L  V LMKTPLE +I+ RK
Sbjct: 463 EGKFPDMSEELSFVLLMKTPLEAKITARK 491


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  110 bits (276), Expect(2) = 8e-30
 Identities = 51/59 (86%), Positives = 55/59 (93%)
 Frame = -2

Query: 538 FMPERFLQGEDGSANNKAMEQSFLPFGAGMRICAGMNLGKLQIAFALANLVNAFKWSCV 362
           FMPER+LQGEDGS  +KAMEQS LPFGAGMRICAGM+LGKLQ+AF LANLVNAFKWSCV
Sbjct: 418 FMPERYLQGEDGSVTHKAMEQSLLPFGAGMRICAGMDLGKLQVAFVLANLVNAFKWSCV 476



 Score = 44.7 bits (104), Expect(2) = 8e-30
 Identities = 22/31 (70%), Positives = 26/31 (83%), Gaps = 1/31 (3%)
 Frame = -3

Query: 258 EGKLPDMSEQLFA-VTLMKTPLEGRISTRKV 169
           +GKLPDM+E+LF  + LMKTPLEGRIS R V
Sbjct: 478 DGKLPDMTEELFGLIILMKTPLEGRISPRTV 508


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
           Full=(S)-tetrahydrocolumbamine oxidase
           (methylenedioxy-bridge-forming); AltName:
           Full=Cytochrome P450 719A1 gi|15822820|dbj|BAB68769.1|
           methylenedioxy bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  110 bits (274), Expect(2) = 4e-29
 Identities = 52/59 (88%), Positives = 55/59 (93%)
 Frame = -2

Query: 538 FMPERFLQGEDGSANNKAMEQSFLPFGAGMRICAGMNLGKLQIAFALANLVNAFKWSCV 362
           FMPERFL+GE G+A NKAMEQSFLPF AGMRICAGM+LGKLQ AFALANLVNAFKWSCV
Sbjct: 402 FMPERFLEGETGTAYNKAMEQSFLPFSAGMRICAGMDLGKLQFAFALANLVNAFKWSCV 460



 Score = 43.1 bits (100), Expect(2) = 4e-29
 Identities = 21/30 (70%), Positives = 23/30 (76%)
 Frame = -3

Query: 258 EGKLPDMSEQLFAVTLMKTPLEGRISTRKV 169
           EGKLPDM E+L  V LMKTPLE RI+ R V
Sbjct: 462 EGKLPDMGEELSFVLLMKTPLEARIAGRNV 491


>gb|ACO90244.1| canadine synthase [Thalictrum flavum]
          Length = 78

 Score = 90.9 bits (224), Expect(2) = 2e-23
 Identities = 43/47 (91%), Positives = 45/47 (95%)
 Frame = -2

Query: 502 SANNKAMEQSFLPFGAGMRICAGMNLGKLQIAFALANLVNAFKWSCV 362
           SA+NKAMEQSFLPF AGMRICAGM+LGKLQ AFALANLVNAFKWSCV
Sbjct: 1   SASNKAMEQSFLPFSAGMRICAGMDLGKLQFAFALANLVNAFKWSCV 47



 Score = 43.1 bits (100), Expect(2) = 2e-23
 Identities = 20/29 (68%), Positives = 23/29 (79%)
 Frame = -3

Query: 258 EGKLPDMSEQLFAVTLMKTPLEGRISTRK 172
           EGK PDMSE+L  V LMKTPLE +I+ RK
Sbjct: 49  EGKFPDMSEELSFVLLMKTPLEAKITARK 77