BLASTX nr result
ID: Coptis24_contig00021146
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00021146
(550 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAC67200.1| putative retroelement pol polyprotein [Arabidopsi... 114 7e-29
emb|CAN78447.1| hypothetical protein VITISV_026810 [Vitis vinifera] 129 4e-28
emb|CAC37623.1| copia-like polyprotein [Arabidopsis thaliana] 120 5e-28
gb|AAT93988.1| putative polyprotein [Oryza sativa Japonica Group] 121 6e-28
gb|AAD21687.1| Strong similarity to gi|3600044 T12H20.12 proteas... 117 3e-27
>gb|AAC67200.1| putative retroelement pol polyprotein [Arabidopsis thaliana]
Length = 1402
Score = 114 bits (284), Expect(2) = 7e-29
Identities = 51/122 (41%), Positives = 75/122 (61%), Gaps = 1/122 (0%)
Frame = +1
Query: 187 CKACAMGKSTRLSFITHEHRTTAPFDLIHSDVW-MSPISIVSGYCYYVLFTNDFTSYSWV 363
C+AC +GKSTRL F++ + P + +H D+W SPI+ V G+ YY +F + ++ +SW+
Sbjct: 498 CEACQLGKSTRLPFVSSSFTSNRPLERVHCDLWGPSPITSVQGFRYYAVFIDHYSRFSWI 557
Query: 364 FPMKLKSEVFSHFVHLFNSIENQFSAIIKCFQSDGGGEYDNSSFHNFCTNKGIHHRFSCP 543
+P+KLKS+ ++ FV +ENQ + I FQ DGGGE+ N F N GI S P
Sbjct: 558 YPLKLKSDFYNIFVAFHKLVENQLNHKISVFQCDGGGEFVNHKFLQHLQNHGIQQHISYP 617
Query: 544 HT 549
HT
Sbjct: 618 HT 619
Score = 38.5 bits (88), Expect(2) = 7e-29
Identities = 21/59 (35%), Positives = 31/59 (52%), Gaps = 3/59 (5%)
Frame = +2
Query: 17 IQGQCKDGLYPIKPSCSFQALTTS---SLLPTLWHRSLGHPSSPILRKLFNNKCLVSVN 184
I G DGLY +K F+A ++ S +WHR LGHP +L++L +S+N
Sbjct: 434 IMGSTCDGLYCLKDDSQFKAFFSTRQQSASDEVWHRRLGHPHPQVLQQLVKTNS-ISIN 491
>emb|CAN78447.1| hypothetical protein VITISV_026810 [Vitis vinifera]
Length = 1171
Score = 129 bits (323), Expect = 4e-28
Identities = 66/173 (38%), Positives = 101/173 (58%), Gaps = 6/173 (3%)
Frame = +1
Query: 49 HQTELFLSSTYDI*FVAHTLASQFGA------SLIPYSSKII**QMFSQCQFCKACAMGK 210
H++ LS+T + A T S+ G + + +S+K+ ++ +FC AC +GK
Sbjct: 441 HKSLTCLSTTIGVRANADTWHSRLGHPSSVIFNSLFHSNKLSVKGSSTKLEFCSACQLGK 500
Query: 211 STRLSFITHEHRTTAPFDLIHSDVWMSPISIVSGYCYYVLFTNDFTSYSWVFPMKLKSEV 390
+ +L F +++ P LIHSDVW+SP+ G YYVLF +D++ YSW++P+ KS+V
Sbjct: 501 AKQLPFPESSRQSSVPLALIHSDVWVSPVQSTGGCSYYVLFIDDYSRYSWLYPLHRKSDV 560
Query: 391 FSHFVHLFNSIENQFSAIIKCFQSDGGGEYDNSSFHNFCTNKGIHHRFSCPHT 549
F+ FV E FS IK Q+D GGE+ ++ F F T +GI HR +CPHT
Sbjct: 561 FATFVKFKTIAEKLFSTSIKQIQTDNGGEFTSNQFKQFLTAQGIFHRLTCPHT 613
>emb|CAC37623.1| copia-like polyprotein [Arabidopsis thaliana]
Length = 1466
Score = 120 bits (301), Expect(2) = 5e-28
Identities = 54/122 (44%), Positives = 75/122 (61%), Gaps = 1/122 (0%)
Frame = +1
Query: 187 CKACAMGKSTRLSFITHEHRTTAPFDLIHSDVW-MSPISIVSGYCYYVLFTNDFTSYSWV 363
C+ C MGKSTRL F + + R P D +H D+W SP+ G+ YY +F +DF+ +SW
Sbjct: 490 CEPCQMGKSTRLQFFSSDFRALKPLDRVHCDLWGPSPVVSNQGFKYYAVFVDDFSRFSWF 549
Query: 364 FPMKLKSEVFSHFVHLFNSIENQFSAIIKCFQSDGGGEYDNSSFHNFCTNKGIHHRFSCP 543
FP+++KS+ S F+ +ENQ IK FQSDGGGE+ ++ GIHHR SCP
Sbjct: 550 FPLRMKSKFISVFIAYQKLVENQLGTKIKEFQSDGGGEFTSNKLKEHFREHGIHHRISCP 609
Query: 544 HT 549
+T
Sbjct: 610 YT 611
Score = 29.3 bits (64), Expect(2) = 5e-28
Identities = 22/64 (34%), Positives = 32/64 (50%), Gaps = 3/64 (4%)
Frame = +2
Query: 20 QGQCKDGLYPIKPSCSFQALTTSSLLPT---LWHRSLGHPSSPILRKLFNNKCLVSVNFV 190
+G +GLY ++ S F AL ++ WH LGH +S IL++L K + VN
Sbjct: 427 KGPRNNGLYMLENS-EFVALYSNRQCAASMETWHHRLGHSNSKILQQLLTRK-EIQVNKS 484
Query: 191 RPVP 202
R P
Sbjct: 485 RTSP 488
>gb|AAT93988.1| putative polyprotein [Oryza sativa Japonica Group]
Length = 1480
Score = 121 bits (303), Expect(2) = 6e-28
Identities = 53/126 (42%), Positives = 81/126 (64%)
Frame = +1
Query: 172 SQCQFCKACAMGKSTRLSFITHEHRTTAPFDLIHSDVWMSPISIVSGYCYYVLFTNDFTS 351
S + C AC +GK TRLSF T++PF+L+H DVW SP+ +SG+ YY++ +DFT
Sbjct: 516 SNNKLCHACHLGKHTRLSFSKSSSSTSSPFELVHCDVWTSPVLSLSGFKYYLVVLDDFTH 575
Query: 352 YSWVFPMKLKSEVFSHFVHLFNSIENQFSAIIKCFQSDGGGEYDNSSFHNFCTNKGIHHR 531
+ W FP++ KS+V H + ++ QFS I+CFQ+D G ++ N + +F ++GI R
Sbjct: 576 FCWTFPLRHKSDVHQHLLEFVAYVKTQFSLPIRCFQADNGTKFVNHATTSFFASRGIVLR 635
Query: 532 FSCPHT 549
SCP+T
Sbjct: 636 LSCPYT 641
Score = 28.1 bits (61), Expect(2) = 6e-28
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 3/30 (10%)
Frame = +2
Query: 74 ALTTSSLLP---TLWHRSLGHPSSPILRKL 154
A+T S L TLWH LGHPS ++ L
Sbjct: 476 AITAHSFLAKSSTLWHHRLGHPSPAAVQTL 505
>gb|AAD21687.1| Strong similarity to gi|3600044 T12H20.12 protease homolog from
Arabidopsis thaliana BAC gb|AF080119 and is a member of
the reverse transcriptase family PF|00078 [Arabidopsis
thaliana]
Length = 1415
Score = 117 bits (294), Expect(2) = 3e-27
Identities = 54/122 (44%), Positives = 74/122 (60%), Gaps = 1/122 (0%)
Frame = +1
Query: 187 CKACAMGKSTRLSFITHEHRTTAPFDLIHSDVW-MSPISIVSGYCYYVLFTNDFTSYSWV 363
C+ C MGKS+RL F+ + R P D IH D+W SP+ G YY +F +D++ YSW
Sbjct: 488 CEPCQMGKSSRLPFLISDSRVLHPLDRIHCDLWGPSPVVSNQGLKYYAIFVDDYSRYSWF 547
Query: 364 FPMKLKSEVFSHFVHLFNSIENQFSAIIKCFQSDGGGEYDNSSFHNFCTNKGIHHRFSCP 543
+P+ KSE S F+ +ENQ + IK FQSDGGGE+ ++ + GIHHR SCP
Sbjct: 548 YPLHNKSEFLSVFISFQKLVENQLNTKIKVFQSDGGGEFVSNKLKTHLSEHGIHHRISCP 607
Query: 544 HT 549
+T
Sbjct: 608 YT 609
Score = 29.3 bits (64), Expect(2) = 3e-27
Identities = 20/63 (31%), Positives = 33/63 (52%), Gaps = 3/63 (4%)
Frame = +2
Query: 23 GQCKDGLYPIKPSCSFQALTTS---SLLPTLWHRSLGHPSSPILRKLFNNKCLVSVNFVR 193
G ++GLY ++ F AL ++ + +WH LGH +S L+ L N+K + +N R
Sbjct: 426 GPRRNGLYVLENQ-EFVALYSNRQCAATEEVWHHRLGHANSKALQHLQNSKA-IQINKSR 483
Query: 194 PVP 202
P
Sbjct: 484 TSP 486