BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00020777
         (491 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284809.1| PREDICTED: probable E3 ubiquitin-protein lig...   257   8e-67
emb|CAN60415.1| hypothetical protein VITISV_023540 [Vitis vinifera]   257   8e-67
ref|XP_002528573.1| Ran GTPase binding protein, putative [Ricinu...   248   4e-64
ref|XP_002329104.1| predicted protein [Populus trichocarpa] gi|2...   247   6e-64
ref|XP_002316910.1| predicted protein [Populus trichocarpa] gi|2...   247   8e-64

>ref|XP_002284809.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC2 [Vitis
           vinifera] gi|302143037|emb|CBI20332.3| unnamed protein
           product [Vitis vinifera]
          Length = 492

 Score =  257 bits (656), Expect = 8e-67
 Identities = 121/147 (82%), Positives = 130/147 (88%)
 Frame = -2

Query: 442 MQSGEVFTCGDNSSYCCGHGEGGHAIFRPRRVEALKGVSCKQVATGLSFTAFLTRQGHVY 263
           MQSGEVFTCGDNSS+CCGHG+    IFRPR VEALKGV CKQVA GLSFT FLT +GHVY
Sbjct: 179 MQSGEVFTCGDNSSFCCGHGDTVRPIFRPRLVEALKGVPCKQVAAGLSFTVFLTMKGHVY 238

Query: 262 TCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPSYTLAVMDDGTVYSFGSGTN 83
           TCG+N HGQLGHGD VDR TPKIVE LEGVG VVQIAAGPSY +AV DDGTV+SFGSG+N
Sbjct: 239 TCGTNTHGQLGHGDTVDRRTPKIVELLEGVGPVVQIAAGPSYAMAVTDDGTVHSFGSGSN 298

Query: 82  FCLGHGEQHNELQPRAIQSFKRKNIHV 2
           FCLGHGEQHNEL+PRAIQSF+RK IHV
Sbjct: 299 FCLGHGEQHNELRPRAIQSFRRKTIHV 325



 Score = 76.3 bits (186), Expect = 2e-12
 Identities = 42/115 (36%), Positives = 62/115 (53%), Gaps = 2/115 (1%)
 Frame = -2

Query: 433 GEVFTCGDNSSYCCGHGEGGHAIFRPRRVEAL--KGVSCKQVATGLSFTAFLTRQGHVYT 260
           G V + G  S++C GHGE  H   RPR +++   K +   +++ G      L   G VYT
Sbjct: 288 GTVHSFGSGSNFCLGHGEQ-HNELRPRAIQSFRRKTIHVVRISAGDEHVVALDSSGFVYT 346

Query: 259 CGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPSYTLAVMDDGTVYSFG 95
            G    G LGHGD +D+ TP+I+ +L+     VQ+ A    T  ++D G+VY FG
Sbjct: 347 WGKGYCGALGHGDEIDKTTPEILSSLKS-HLAVQVCARKRKTFVLVDTGSVYGFG 400



 Score = 55.1 bits (131), Expect = 6e-06
 Identities = 31/101 (30%), Positives = 51/101 (50%), Gaps = 1/101 (0%)
 Frame = -2

Query: 319 QVATGLSFTAFLTRQGHVYTCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPS 140
           Q+ TG   T  L +   VY+CGS+  G LGHG    +           +  V Q++A  +
Sbjct: 115 QITTGRYHT-LLIKDSSVYSCGSSLCGVLGHGPETTQCVAFSRINFPPLAHVAQVSASHN 173

Query: 139 YTLAVMDDGTVYSFGSGTNFCLGHGEQHNEL-QPRAIQSFK 20
           +   VM  G V++ G  ++FC GHG+    + +PR +++ K
Sbjct: 174 HAAFVMQSGEVFTCGDNSSFCCGHGDTVRPIFRPRLVEALK 214


>emb|CAN60415.1| hypothetical protein VITISV_023540 [Vitis vinifera]
          Length = 511

 Score =  257 bits (656), Expect = 8e-67
 Identities = 121/147 (82%), Positives = 130/147 (88%)
 Frame = -2

Query: 442 MQSGEVFTCGDNSSYCCGHGEGGHAIFRPRRVEALKGVSCKQVATGLSFTAFLTRQGHVY 263
           MQSGEVFTCGDNSS+CCGHG+    IFRPR VEALKGV CKQVA GLSFT FLT +GHVY
Sbjct: 179 MQSGEVFTCGDNSSFCCGHGDTVRPIFRPRLVEALKGVPCKQVAAGLSFTVFLTMKGHVY 238

Query: 262 TCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPSYTLAVMDDGTVYSFGSGTN 83
           TCG+N HGQLGHGD VDR TPKIVE LEGVG VVQIAAGPSY +AV DDGTV+SFGSG+N
Sbjct: 239 TCGTNTHGQLGHGDTVDRRTPKIVELLEGVGPVVQIAAGPSYAMAVTDDGTVHSFGSGSN 298

Query: 82  FCLGHGEQHNELQPRAIQSFKRKNIHV 2
           FCLGHGEQHNEL+PRAIQSF+RK IHV
Sbjct: 299 FCLGHGEQHNELRPRAIQSFRRKTIHV 325



 Score = 76.3 bits (186), Expect = 2e-12
 Identities = 42/115 (36%), Positives = 62/115 (53%), Gaps = 2/115 (1%)
 Frame = -2

Query: 433 GEVFTCGDNSSYCCGHGEGGHAIFRPRRVEAL--KGVSCKQVATGLSFTAFLTRQGHVYT 260
           G V + G  S++C GHGE  H   RPR +++   K +   +++ G      L   G VYT
Sbjct: 288 GTVHSFGSGSNFCLGHGEQ-HNELRPRAIQSFRRKTIHVVRISAGDEHVVALDSSGFVYT 346

Query: 259 CGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPSYTLAVMDDGTVYSFG 95
            G    G LGHGD +D+ TP+I+ +L+     VQ+ A    T  ++D G+VY FG
Sbjct: 347 WGKGYCGALGHGDEIDKTTPEILSSLKS-HLAVQVCARKRKTFVLVDTGSVYGFG 400



 Score = 55.1 bits (131), Expect = 6e-06
 Identities = 31/101 (30%), Positives = 51/101 (50%), Gaps = 1/101 (0%)
 Frame = -2

Query: 319 QVATGLSFTAFLTRQGHVYTCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPS 140
           Q+ TG   T  L +   VY+CGS+  G LGHG    +           +  V Q++A  +
Sbjct: 115 QITTGRYHT-LLIKDSSVYSCGSSLCGVLGHGPETTQCVAFSRINFPPLAHVAQVSASHN 173

Query: 139 YTLAVMDDGTVYSFGSGTNFCLGHGEQHNEL-QPRAIQSFK 20
           +   VM  G V++ G  ++FC GHG+    + +PR +++ K
Sbjct: 174 HAAFVMQSGEVFTCGDNSSFCCGHGDTVRPIFRPRLVEALK 214


>ref|XP_002528573.1| Ran GTPase binding protein, putative [Ricinus communis]
           gi|223532017|gb|EEF33828.1| Ran GTPase binding protein,
           putative [Ricinus communis]
          Length = 497

 Score =  248 bits (633), Expect = 4e-64
 Identities = 111/147 (75%), Positives = 126/147 (85%)
 Frame = -2

Query: 442 MQSGEVFTCGDNSSYCCGHGEGGHAIFRPRRVEALKGVSCKQVATGLSFTAFLTRQGHVY 263
           ++SGEVFTCGDNSS+CCGH +    IFRPR VEALKGV CKQVA GL+FT FLTR GHVY
Sbjct: 179 LESGEVFTCGDNSSFCCGHQDTSRPIFRPRFVEALKGVPCKQVAAGLNFTVFLTRTGHVY 238

Query: 262 TCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPSYTLAVMDDGTVYSFGSGTN 83
           +CG+N HGQLGHGD +DRPTPKI+E+ EG+G V QIAAGPSY LAV D G VYSFGSG+N
Sbjct: 239 SCGTNTHGQLGHGDTLDRPTPKIIESFEGIGSVAQIAAGPSYVLAVTDSGVVYSFGSGSN 298

Query: 82  FCLGHGEQHNELQPRAIQSFKRKNIHV 2
           FCLGHGEQHNE  PRAIQ+F+RKN+HV
Sbjct: 299 FCLGHGEQHNEFHPRAIQTFRRKNLHV 325


>ref|XP_002329104.1| predicted protein [Populus trichocarpa] gi|222869773|gb|EEF06904.1|
           predicted protein [Populus trichocarpa]
          Length = 492

 Score =  247 bits (631), Expect = 6e-64
 Identities = 113/147 (76%), Positives = 128/147 (87%)
 Frame = -2

Query: 442 MQSGEVFTCGDNSSYCCGHGEGGHAIFRPRRVEALKGVSCKQVATGLSFTAFLTRQGHVY 263
           +QSGEVFTCGDNSS+CCGH +    IFRPR VEALK VSCKQV+ GL+FT FLTRQG +Y
Sbjct: 179 LQSGEVFTCGDNSSFCCGHKDTNRPIFRPRLVEALKAVSCKQVSVGLNFTVFLTRQGQLY 238

Query: 262 TCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPSYTLAVMDDGTVYSFGSGTN 83
           TCGSN HGQLGHGD +DRPTPKI+E LEG+G VVQIAAGPSY LAV D+G VYSFG G+N
Sbjct: 239 TCGSNTHGQLGHGDTLDRPTPKIIELLEGIGPVVQIAAGPSYVLAVTDNGVVYSFGYGSN 298

Query: 82  FCLGHGEQHNELQPRAIQSFKRKNIHV 2
           FCLGHGEQH+E QPRAIQ+F+RK+IHV
Sbjct: 299 FCLGHGEQHDEFQPRAIQTFRRKDIHV 325



 Score = 76.6 bits (187), Expect = 2e-12
 Identities = 45/126 (35%), Positives = 64/126 (50%), Gaps = 2/126 (1%)
 Frame = -2

Query: 436 SGEVFTCGDNSSYCCGHGEGGHAIFRPRRVEAL--KGVSCKQVATGLSFTAFLTRQGHVY 263
           +G V++ G  S++C GHGE  H  F+PR ++    K +    V+ G      L   G VY
Sbjct: 287 NGVVYSFGYGSNFCLGHGEQ-HDEFQPRAIQTFRRKDIHVVHVSAGDEHAVALDSSGFVY 345

Query: 262 TCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPSYTLAVMDDGTVYSFGSGTN 83
           + G    G LGHGD +D+  P +V  L+     VQ  A    T  ++DDG+VY FG    
Sbjct: 346 SWGKGYCGALGHGDEIDKTLPSLVNCLKS-RLAVQACARKRKTFVLVDDGSVYGFG---- 400

Query: 82  FCLGHG 65
            C+G G
Sbjct: 401 -CMGFG 405



 Score = 57.0 bits (136), Expect = 2e-06
 Identities = 30/108 (27%), Positives = 53/108 (49%), Gaps = 1/108 (0%)
 Frame = -2

Query: 340 LKGVSCKQVATGLSFTAFLTRQGHVYTCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVV 161
           +K  +C    T   +   L R   VY+CGS+  G LGHG    +           +  VV
Sbjct: 107 VKTSACNMQVTTGRYHTLLIRDSSVYSCGSSLCGVLGHGAETTQCVAFTRIRFPSLANVV 166

Query: 160 QIAAGPSYTLAVMDDGTVYSFGSGTNFCLGHGEQHNEL-QPRAIQSFK 20
           Q++A  ++   V+  G V++ G  ++FC GH + +  + +PR +++ K
Sbjct: 167 QVSASHNHAAFVLQSGEVFTCGDNSSFCCGHKDTNRPIFRPRLVEALK 214


>ref|XP_002316910.1| predicted protein [Populus trichocarpa] gi|222859975|gb|EEE97522.1|
           predicted protein [Populus trichocarpa]
          Length = 478

 Score =  247 bits (630), Expect = 8e-64
 Identities = 113/147 (76%), Positives = 127/147 (86%)
 Frame = -2

Query: 442 MQSGEVFTCGDNSSYCCGHGEGGHAIFRPRRVEALKGVSCKQVATGLSFTAFLTRQGHVY 263
           +QSGEVFTCGDNSS+CCGH +    IFRPR VEALKGV CKQVA GL+FT FLTRQG +Y
Sbjct: 179 LQSGEVFTCGDNSSFCCGHNDTNRPIFRPRLVEALKGVPCKQVAVGLNFTVFLTRQGQLY 238

Query: 262 TCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPSYTLAVMDDGTVYSFGSGTN 83
           TCG+N HGQLGHGD  DRPTPKI+E LEGVG VVQIAAGPSY LAV D+G VYSFGSG+N
Sbjct: 239 TCGTNTHGQLGHGDTQDRPTPKIIELLEGVGSVVQIAAGPSYVLAVTDNGVVYSFGSGSN 298

Query: 82  FCLGHGEQHNELQPRAIQSFKRKNIHV 2
            CLGHGEQH+E +PRAIQ+F+RK+IHV
Sbjct: 299 LCLGHGEQHDEFRPRAIQTFRRKDIHV 325



 Score = 77.4 bits (189), Expect = 1e-12
 Identities = 48/148 (32%), Positives = 72/148 (48%), Gaps = 5/148 (3%)
 Frame = -2

Query: 436 SGEVFTCGDNSSYCCGHGEGGHAIFRPRRVEAL--KGVSCKQVATGLSFTAFLTRQGHVY 263
           +G V++ G  S+ C GHGE  H  FRPR ++    K +    V+ G      L   G VY
Sbjct: 287 NGVVYSFGSGSNLCLGHGEQ-HDEFRPRAIQTFRRKDIHVVHVSAGDEHAVALDSSGFVY 345

Query: 262 TCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPSYTLAVMDDGTVYSFGSGTN 83
           + G    G LGHGD +D+  P +V  L+     VQ+ A    T  ++D G+VY FG    
Sbjct: 346 SWGKGYCGALGHGDEIDKTLPALVNCLKS-HLAVQVCARKRKTFVLVDGGSVYGFGWMGF 404

Query: 82  FCLG---HGEQHNELQPRAIQSFKRKNI 8
             LG    G     ++PR + S + +++
Sbjct: 405 GSLGFPDRGLSDKVMRPRVLDSLRARHV 432



 Score = 57.4 bits (137), Expect = 1e-06
 Identities = 31/101 (30%), Positives = 52/101 (51%), Gaps = 1/101 (0%)
 Frame = -2

Query: 319 QVATGLSFTAFLTRQGHVYTCGSNAHGQLGHGDNVDRPTPKIVEALEGVGCVVQIAAGPS 140
           QV TG   T  L R   VY+CGS+  G LGHG    +           +  V+Q++A  +
Sbjct: 115 QVTTGRYHT-LLIRDSSVYSCGSSLCGVLGHGAETTQCVAFTRIRFPSLAYVIQVSASHN 173

Query: 139 YTLAVMDDGTVYSFGSGTNFCLGHGEQHNEL-QPRAIQSFK 20
           +   V+  G V++ G  ++FC GH + +  + +PR +++ K
Sbjct: 174 HAAFVLQSGEVFTCGDNSSFCCGHNDTNRPIFRPRLVEALK 214