BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00020354
         (1014 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAE16981.1| delta7 sterol C-5 desaturase [Zinnia elegans]         485   e-135
gb|AAD20458.1| sterol-C5(6)-desaturase homolog [Nicotiana tabacum]    483   e-134
gb|ABD96921.1| hypothetical protein [Cleome spinosa]                  482   e-134
ref|XP_002284080.1| PREDICTED: delta(7)-sterol-C5(6)-desaturase ...   479   e-133
ref|XP_002516756.1| sterol desaturase, putative [Ricinus communi...   474   e-131

>dbj|BAE16981.1| delta7 sterol C-5 desaturase [Zinnia elegans]
          Length = 271

 Score =  485 bits (1249), Expect = e-135
 Identities = 215/266 (80%), Positives = 242/266 (90%)
 Frame = -3

Query: 922 LKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLISGLLWCLYIYY 743
           L LF+ ET++YNQIVLG ++P  KL+ PLPH+ QTW+RNYI GTL+YLISG LWC YIY+
Sbjct: 5   LSLFVTETTWYNQIVLGTLLP-EKLWTPLPHFLQTWLRNYIAGTLVYLISGFLWCFYIYH 63

Query: 742 LKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRCYSRISDVGWS 563
           LKRNVY+PKDAIP+ KAM LQI VAMKAMPWYCAL  VSEY VENGWTRC+SRISDVGW+
Sbjct: 64  LKRNVYVPKDAIPTRKAMYLQIYVAMKAMPWYCALRAVSEYMVENGWTRCFSRISDVGWA 123

Query: 562 AYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 383
           +Y+ Y+ +YL+FVEFGIYWMHRELHDIKPLYK+LHATHHI+NKQNTLSPFAGLAFH +DG
Sbjct: 124 SYISYMTLYLIFVEFGIYWMHRELHDIKPLYKWLHATHHIHNKQNTLSPFAGLAFHAIDG 183

Query: 382 ILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 203
           ILQA+PHVIALFL+PTHF THI LLF+EAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR
Sbjct: 184 ILQAIPHVIALFLVPTHFMTHILLLFMEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 243

Query: 202 HNYGHYTVWMDWMFGTLRDPVSDVTK 125
           HNYGHYTVWMDWMFGTLRDP+ +  K
Sbjct: 244 HNYGHYTVWMDWMFGTLRDPLEEEAK 269


>gb|AAD20458.1| sterol-C5(6)-desaturase homolog [Nicotiana tabacum]
          Length = 271

 Score =  483 bits (1244), Expect = e-134
 Identities = 216/266 (81%), Positives = 239/266 (89%)
 Frame = -3

Query: 922 LKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLISGLLWCLYIYY 743
           L LF++ETSFYN++VLG  +P S  + PLPHWFQ W+RNYIGG LLY ISG LWC YIY 
Sbjct: 5   LNLFIEETSFYNRVVLGTFLPES-WWGPLPHWFQGWLRNYIGGVLLYFISGFLWCFYIYR 63

Query: 742 LKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRCYSRISDVGWS 563
           LKRNVY+PKDAIPS +AMLLQI VAMKAMP+YCALP++SEY + NGWT+C+SRISDVGW 
Sbjct: 64  LKRNVYIPKDAIPSNRAMLLQIGVAMKAMPFYCALPSLSEYMIVNGWTKCFSRISDVGWL 123

Query: 562 AYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 383
           +YL+Y+A+YLV VEFGIYWMHRELHDIK LYKYLHATHHIYNKQNTLSPFAGLAFHPLDG
Sbjct: 124 SYLIYMAVYLVIVEFGIYWMHRELHDIKLLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 382 ILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 203
           ILQA+PHV+ALFL+P HFTTHIALLFIEAIWTANIHDC H K WPVMGAGYHTIHHTTYR
Sbjct: 184 ILQAVPHVVALFLLPEHFTTHIALLFIEAIWTANIHDCKHAKVWPVMGAGYHTIHHTTYR 243

Query: 202 HNYGHYTVWMDWMFGTLRDPVSDVTK 125
           HNYGHYT+WMDWMFGTLRDPV D  K
Sbjct: 244 HNYGHYTIWMDWMFGTLRDPVEDEVK 269


>gb|ABD96921.1| hypothetical protein [Cleome spinosa]
          Length = 307

 Score =  482 bits (1240), Expect = e-134
 Identities = 211/273 (77%), Positives = 242/273 (88%)
 Frame = -3

Query: 952 KNTKMENSTSLKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLIS 773
           K    E S  +  F+DETSFYN+IVLG+++P + L++PLPH+ QTW+RNY+ GTLLYLIS
Sbjct: 25  KTAAAEQSHLMMQFVDETSFYNRIVLGHLLP-ANLWDPLPHFLQTWLRNYLAGTLLYLIS 83

Query: 772 GLLWCLYIYYLKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRC 593
           GL+WC YIYYLK NVY+PKDAIP+ KAMLLQI VAMKAMPWY  LPTVSE+ +E GWTRC
Sbjct: 84  GLIWCFYIYYLKHNVYVPKDAIPTRKAMLLQIYVAMKAMPWYTLLPTVSEHMIERGWTRC 143

Query: 592 YSRISDVGWSAYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPF 413
           +S I D GW+ YLVYLA YLV VEFGIYW+HRELHDIKPLYKYLHATHHIYNKQNTLSPF
Sbjct: 144 FSEIDDYGWTLYLVYLAFYLVIVEFGIYWVHRELHDIKPLYKYLHATHHIYNKQNTLSPF 203

Query: 412 AGLAFHPLDGILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAG 233
           AGLAFHPLDGILQA+PHVIALF++P HFTTH+ALLF+EA+WTANIHDCIHG  WPVMGAG
Sbjct: 204 AGLAFHPLDGILQAVPHVIALFIVPIHFTTHLALLFMEAVWTANIHDCIHGNIWPVMGAG 263

Query: 232 YHTIHHTTYRHNYGHYTVWMDWMFGTLRDPVSD 134
           YHTIHHTTY+HNYGHYT+WMDWMFG+LRDP+ +
Sbjct: 264 YHTIHHTTYKHNYGHYTIWMDWMFGSLRDPIEE 296


>ref|XP_002284080.1| PREDICTED: delta(7)-sterol-C5(6)-desaturase [Vitis vinifera]
           gi|296087259|emb|CBI33633.3| unnamed protein product
           [Vitis vinifera]
          Length = 271

 Score =  479 bits (1233), Expect = e-133
 Identities = 218/260 (83%), Positives = 234/260 (90%)
 Frame = -3

Query: 922 LKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLISGLLWCLYIYY 743
           L LF+ ETSFYN+IVLG  +P +KL+E  PH+ QTW+RNYIGGTL+YL+SG LWC YIYY
Sbjct: 5   LHLFVAETSFYNRIVLGTFLP-AKLWETWPHFVQTWLRNYIGGTLIYLVSGFLWCFYIYY 63

Query: 742 LKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRCYSRISDVGWS 563
           LKRNVY+PKDAIP+T+AM LQI VAMKAMPWY ALPT+SEY VENGWTRCYS ISDVGW 
Sbjct: 64  LKRNVYVPKDAIPTTRAMYLQIYVAMKAMPWYTALPTLSEYMVENGWTRCYSSISDVGWP 123

Query: 562 AYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 383
           AY V LA+YLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG
Sbjct: 124 AYFVNLAVYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 183

Query: 382 ILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 203
           ILQALPHV+ALFL  THF TH+ LLFIEAIWTANIHDCI GK WPVMGA YHTIHHTTYR
Sbjct: 184 ILQALPHVLALFLFRTHFLTHVLLLFIEAIWTANIHDCIDGKIWPVMGAAYHTIHHTTYR 243

Query: 202 HNYGHYTVWMDWMFGTLRDP 143
           HNYGHYTVWMDWMFGTL DP
Sbjct: 244 HNYGHYTVWMDWMFGTLCDP 263


>ref|XP_002516756.1| sterol desaturase, putative [Ricinus communis]
           gi|223544129|gb|EEF45654.1| sterol desaturase, putative
           [Ricinus communis]
          Length = 276

 Score =  474 bits (1221), Expect = e-131
 Identities = 213/263 (80%), Positives = 234/263 (88%)
 Frame = -3

Query: 922 LKLFLDETSFYNQIVLGNIIPFSKLYEPLPHWFQTWMRNYIGGTLLYLISGLLWCLYIYY 743
           L+ FL+ET+ YN IVLG+++P S  +  LP + QTW+RNYI GTLLY ISG LWC YIYY
Sbjct: 9   LQYFLEETAMYNNIVLGHLLP-SGWWATLPRFLQTWLRNYIAGTLLYFISGFLWCFYIYY 67

Query: 742 LKRNVYLPKDAIPSTKAMLLQIMVAMKAMPWYCALPTVSEYTVENGWTRCYSRISDVGWS 563
           LKRNVYLPKDAIP+TKAMLLQI VAMKAMPWYCALP++SEY VENGWT+C+S ISDVG  
Sbjct: 68  LKRNVYLPKDAIPTTKAMLLQIYVAMKAMPWYCALPSLSEYMVENGWTKCFSYISDVGCF 127

Query: 562 AYLVYLAIYLVFVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 383
            Y++YL  YLV VEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG
Sbjct: 128 GYIIYLIAYLVMVEFGIYWMHRELHDIKPLYKYLHATHHIYNKQNTLSPFAGLAFHPLDG 187

Query: 382 ILQALPHVIALFLIPTHFTTHIALLFIEAIWTANIHDCIHGKFWPVMGAGYHTIHHTTYR 203
           ILQA+PHVIALF++PTHF THI LLF+EAIWTANIHDCIHGK WPVMGAGYHTIHHTTYR
Sbjct: 188 ILQAVPHVIALFIVPTHFRTHIGLLFLEAIWTANIHDCIHGKLWPVMGAGYHTIHHTTYR 247

Query: 202 HNYGHYTVWMDWMFGTLRDPVSD 134
           HNYGHYT+WMDWM GTLRDP  D
Sbjct: 248 HNYGHYTIWMDWMLGTLRDPEED 270