BLASTX nr result
ID: Coptis24_contig00019388
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00019388
(1171 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003552911.1| PREDICTED: taxadiene 5-alpha hydroxylase-lik... 399 e-109
ref|XP_002275049.2| PREDICTED: cytochrome P450 716B1 [Vitis vini... 348 1e-93
emb|CBI25367.3| unnamed protein product [Vitis vinifera] 348 1e-93
emb|CAN70350.1| hypothetical protein VITISV_012582 [Vitis vinifera] 348 1e-93
ref|XP_002325485.1| cytochrome P450 [Populus trichocarpa] gi|222... 313 4e-83
>ref|XP_003552911.1| PREDICTED: taxadiene 5-alpha hydroxylase-like [Glycine max]
Length = 483
Score = 399 bits (1026), Expect = e-109
Identities = 187/340 (55%), Positives = 260/340 (76%)
Frame = -2
Query: 1020 KVKNLPKGSLGRLPYIGETLNFLRAQNRNQGAEWISERVTKYGPVFKTSLMGAPTVIITG 841
+ KN+PKGSLG P IGETL+FL+AQ +++G+ W+ ER++KYGP+FKTSLMG PTV + G
Sbjct: 29 QTKNVPKGSLG-YPIIGETLSFLKAQRQDKGSVWLEERISKYGPIFKTSLMGFPTVFVIG 87
Query: 840 QAGNKFVLGSDDEIFASKQPKSISEIAGKHNLFELTGARYKIIKAAMVSFLKPECLQHYV 661
Q GNKFVLGS D++ +SK+P ++ +I G+ +L ELTG RY+++K M+ FLKPECLQ+YV
Sbjct: 88 QEGNKFVLGSPDDLLSSKKPLTLRKILGRQSLVELTGPRYRLVKGEMLKFLKPECLQNYV 147
Query: 660 KHMDDLIAKKLHSETKDRDTVKAVDLMKIVTFDVACTVLFGIHDETIKNDLLSDFTIALT 481
K MD+L+ L E ++ + ++AV MK +++++AC +LF I DE K L DFT+A
Sbjct: 148 KEMDELVNATLLREFRENEIIRAVVFMKKLSYEIACNLLFDIKDEHTKEALFVDFTLAFK 207
Query: 480 AVWSLPINFPGTHFRKGLEARGRIIRRMSPIIMKKKEDLTKGIVYPKTDVICSLLALTED 301
A+ SLPIN PGT F +G AR RI+ RM PI+ K++E+L+KG++ D++ LLAL ++
Sbjct: 208 AIHSLPINLPGTTFWRGQRARARIVDRMIPILNKRREELSKGVLSSTNDMLSCLLALRDE 267
Query: 300 NEEPITEQEVIDNFFMLMIASHDTSATLLSLMIMKLAKDPKIYEKIREEQLRILSERQGA 121
N +P+ + + DNF L +ASHDTSATL+SLMI KL++D ++Y K+ EEQ+ I+ +R+G
Sbjct: 268 NHQPLDDDLITDNFIFLFVASHDTSATLMSLMIWKLSRDQEVYNKVLEEQMEIIKQREGT 327
Query: 120 NENLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAVKDT 1
E LTW EIQKMKYTWRVAQELMR+IPP+FGSFRKA+KDT
Sbjct: 328 EERLTWAEIQKMKYTWRVAQELMRMIPPLFGSFRKALKDT 367
>ref|XP_002275049.2| PREDICTED: cytochrome P450 716B1 [Vitis vinifera]
Length = 516
Score = 348 bits (893), Expect = 1e-93
Identities = 164/339 (48%), Positives = 242/339 (71%), Gaps = 1/339 (0%)
Frame = -2
Query: 1014 KNLPKGSLGRLPYIGETLNFLRAQNRNQGAEWISERVTKYGPVFKTSLMGAPTVIITGQA 835
K LP GSLG P+IGET++FLRAQ +++ EWI R+ KYGPVFKTSLMG+ V++TGQA
Sbjct: 45 KKLPSGSLG-FPFIGETISFLRAQRQDKTVEWIESRIAKYGPVFKTSLMGSKVVVLTGQA 103
Query: 834 GNKFVLGSDDEIFASKQPKSISEIAGKHNLFELTGARYKIIKAAMVSFLKPECLQHYVKH 655
GN+F+ D S QP S+++I GKH++FEL G R+K+++ A+++FLKPE +Q V
Sbjct: 104 GNRFLFSGSDNGILSNQPMSVAKILGKHSIFELAGTRHKLVRGAIMNFLKPESIQRSVSR 163
Query: 654 MDDLIAKKLHSETKDRDTVKAVDLMKIVTFDVACTVLFGIHDETIKNDLLSDFTIALTAV 475
MD ++ ++L E + +D+V+ V LMK +TF V C++LFG+ D +LL DFT AL
Sbjct: 164 MDSVVQQQLFQELEGKDSVQMVGLMKKITFKVTCSLLFGLPDGKETEELLEDFTTALKGA 223
Query: 474 WSLPINFPGTHFRKGLEARGRIIRRMSPIIMKKKEDLTKGIVYPKTDVICSLLALTEDNE 295
W++P + PGT FRK L+ARGRI ++++ ++ ++K + +G V D+I SL+ L ++N
Sbjct: 224 WTVPWDLPGTVFRKALQARGRICKQLAELVRERKAKIEEGRVDSHEDIISSLITLRQENG 283
Query: 294 EPITEQEVIDNFFMLMIASHDTSATLLSLMIMKLAKDPKIYEKIREEQLRILSERQG-AN 118
+P++E+E+IDN ++IASHDTS LL L+I LA+D ++ +K+ EEQ ++ ++G N
Sbjct: 284 QPLSEEEIIDNLISVVIASHDTSTVLLGLLIRHLARDTEVCKKVLEEQKQVAKAKEGKGN 343
Query: 117 ENLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAVKDT 1
LTW E+Q MKYTWRVAQELMR+ PPV G+F+ A +DT
Sbjct: 344 GKLTWGEVQMMKYTWRVAQELMRMTPPVLGNFKCAWRDT 382
>emb|CBI25367.3| unnamed protein product [Vitis vinifera]
Length = 496
Score = 348 bits (893), Expect = 1e-93
Identities = 164/339 (48%), Positives = 242/339 (71%), Gaps = 1/339 (0%)
Frame = -2
Query: 1014 KNLPKGSLGRLPYIGETLNFLRAQNRNQGAEWISERVTKYGPVFKTSLMGAPTVIITGQA 835
K LP GSLG P+IGET++FLRAQ +++ EWI R+ KYGPVFKTSLMG+ V++TGQA
Sbjct: 45 KKLPSGSLG-FPFIGETISFLRAQRQDKTVEWIESRIAKYGPVFKTSLMGSKVVVLTGQA 103
Query: 834 GNKFVLGSDDEIFASKQPKSISEIAGKHNLFELTGARYKIIKAAMVSFLKPECLQHYVKH 655
GN+F+ D S QP S+++I GKH++FEL G R+K+++ A+++FLKPE +Q V
Sbjct: 104 GNRFLFSGSDNGILSNQPMSVAKILGKHSIFELAGTRHKLVRGAIMNFLKPESIQRSVSR 163
Query: 654 MDDLIAKKLHSETKDRDTVKAVDLMKIVTFDVACTVLFGIHDETIKNDLLSDFTIALTAV 475
MD ++ ++L E + +D+V+ V LMK +TF V C++LFG+ D +LL DFT AL
Sbjct: 164 MDSVVQQQLFQELEGKDSVQMVGLMKKITFKVTCSLLFGLPDGKETEELLEDFTTALKGA 223
Query: 474 WSLPINFPGTHFRKGLEARGRIIRRMSPIIMKKKEDLTKGIVYPKTDVICSLLALTEDNE 295
W++P + PGT FRK L+ARGRI ++++ ++ ++K + +G V D+I SL+ L ++N
Sbjct: 224 WTVPWDLPGTVFRKALQARGRICKQLAELVRERKAKIEEGRVDSHEDIISSLITLRQENG 283
Query: 294 EPITEQEVIDNFFMLMIASHDTSATLLSLMIMKLAKDPKIYEKIREEQLRILSERQG-AN 118
+P++E+E+IDN ++IASHDTS LL L+I LA+D ++ +K+ EEQ ++ ++G N
Sbjct: 284 QPLSEEEIIDNLISVVIASHDTSTVLLGLLIRHLARDTEVCKKVLEEQKQVAKAKEGKGN 343
Query: 117 ENLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAVKDT 1
LTW E+Q MKYTWRVAQELMR+ PPV G+F+ A +DT
Sbjct: 344 GKLTWGEVQMMKYTWRVAQELMRMTPPVLGNFKCAWRDT 382
>emb|CAN70350.1| hypothetical protein VITISV_012582 [Vitis vinifera]
Length = 496
Score = 348 bits (893), Expect = 1e-93
Identities = 164/339 (48%), Positives = 242/339 (71%), Gaps = 1/339 (0%)
Frame = -2
Query: 1014 KNLPKGSLGRLPYIGETLNFLRAQNRNQGAEWISERVTKYGPVFKTSLMGAPTVIITGQA 835
K LP GSLG P+IGET++FLRAQ +++ EWI R+ KYGPVFKTSLMG+ V++TGQA
Sbjct: 45 KKLPSGSLG-FPFIGETISFLRAQRQDKTVEWIESRIAKYGPVFKTSLMGSKVVVLTGQA 103
Query: 834 GNKFVLGSDDEIFASKQPKSISEIAGKHNLFELTGARYKIIKAAMVSFLKPECLQHYVKH 655
GN+F+ D S QP S+++I GKH++FEL G R+K+++ A+++FLKPE +Q V
Sbjct: 104 GNRFLFSGSDNGILSNQPMSVAKILGKHSIFELAGTRHKLVRGAIMNFLKPESIQRSVSR 163
Query: 654 MDDLIAKKLHSETKDRDTVKAVDLMKIVTFDVACTVLFGIHDETIKNDLLSDFTIALTAV 475
MD ++ ++L E + +D+V+ V LMK +TF V C++LFG+ D +LL DFT AL
Sbjct: 164 MDSVVQQQLFQELEGKDSVQMVGLMKKITFKVTCSLLFGLPDGKETEELLEDFTTALKGA 223
Query: 474 WSLPINFPGTHFRKGLEARGRIIRRMSPIIMKKKEDLTKGIVYPKTDVICSLLALTEDNE 295
W++P + PGT FRK L+ARGRI ++++ ++ ++K + +G V D+I SL+ L ++N
Sbjct: 224 WTVPWDLPGTVFRKALQARGRICKQLAQLVRERKAKIEEGRVDSHEDIISSLITLRQENG 283
Query: 294 EPITEQEVIDNFFMLMIASHDTSATLLSLMIMKLAKDPKIYEKIREEQLRILSERQG-AN 118
+P++E+E+IDN ++IASHDTS LL L+I LA+D ++ +K+ EEQ ++ ++G N
Sbjct: 284 QPLSEEEIIDNLISVVIASHDTSTVLLGLLIRHLARDTEVCKKVLEEQKQVAKAKEGKGN 343
Query: 117 ENLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAVKDT 1
LTW E+Q MKYTWRVAQELMR+ PPV G+F+ A +DT
Sbjct: 344 GKLTWGEVQMMKYTWRVAQELMRMTPPVLGNFKCAWRDT 382
>ref|XP_002325485.1| cytochrome P450 [Populus trichocarpa] gi|222862360|gb|EEE99866.1|
cytochrome P450 [Populus trichocarpa]
Length = 502
Score = 313 bits (803), Expect = 4e-83
Identities = 145/337 (43%), Positives = 235/337 (69%)
Frame = -2
Query: 1014 KNLPKGSLGRLPYIGETLNFLRAQNRNQGAEWISERVTKYGPVFKTSLMGAPTVIITGQA 835
K LP GSLG P +GE+++ +RAQ R++ EW+ +R+ K+GP+FKTS+ G TV++TGQA
Sbjct: 45 KKLPPGSLG-FPLVGESISLVRAQKRDKIDEWMWKRIDKFGPIFKTSIFGTKTVVLTGQA 103
Query: 834 GNKFVLGSDDEIFASKQPKSISEIAGKHNLFELTGARYKIIKAAMVSFLKPECLQHYVKH 655
GN+F+ D I + KQPK+I+ I GK++LFE++G+R+K+I+ A+V FLKPE +Q V
Sbjct: 104 GNRFLFSGGDGI-SYKQPKTIASILGKYSLFEISGSRHKLIRGAIVGFLKPERIQKIVGE 162
Query: 654 MDDLIAKKLHSETKDRDTVKAVDLMKIVTFDVACTVLFGIHDETIKNDLLSDFTIALTAV 475
++ L+ ++L E D+VK V MK + F++ C + FGI D K+ L +F++A+
Sbjct: 163 INSLVQQQLSKELDGVDSVKIVPFMKRIAFNITCNIFFGIPDGKEKDTLFEEFSVAVKGC 222
Query: 474 WSLPINFPGTHFRKGLEARGRIIRRMSPIIMKKKEDLTKGIVYPKTDVICSLLALTEDNE 295
W++P++ PGT F + ++AR + + +S II ++K + +G V ++I S L+L ++N+
Sbjct: 223 WAVPLDIPGTVFHRAMQARASLCKILSKIIDERKRQMEEGTVDVNENIIYSFLSLRDEND 282
Query: 294 EPITEQEVIDNFFMLMIASHDTSATLLSLMIMKLAKDPKIYEKIREEQLRILSERQGANE 115
EP+ E+E++D L++ASHD++ LL L++ L++D +IY K+ EEQ ++ + G++
Sbjct: 283 EPLIEEEILDMVLSLIMASHDSTTILLCLLVRLLSRDAEIYNKVLEEQREVIKVKGGSDG 342
Query: 114 NLTWNEIQKMKYTWRVAQELMRIIPPVFGSFRKAVKD 4
+TWNEIQ MKY+WRVAQE+MR PP+FG+FR+ KD
Sbjct: 343 KITWNEIQMMKYSWRVAQEVMRFYPPIFGNFRQITKD 379