BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00018751
         (1633 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002278843.1| PREDICTED: l-2-hydroxyglutarate dehydrogenas...   580   e-163
ref|XP_002308844.1| predicted protein [Populus trichocarpa] gi|2...   578   e-162
ref|XP_003618905.1| L-2-hydroxyglutarate dehydrogenase [Medicago...   570   e-160
ref|XP_003525735.1| PREDICTED: l-2-hydroxyglutarate dehydrogenas...   561   e-157
gb|AFK34722.1| unknown [Lotus japonicus]                              551   e-154

>ref|XP_002278843.1| PREDICTED: l-2-hydroxyglutarate dehydrogenase, mitochondrial [Vitis
            vinifera] gi|297741744|emb|CBI32876.3| unnamed protein
            product [Vitis vinifera]
          Length = 424

 Score =  580 bits (1494), Expect = e-163
 Identities = 294/428 (68%), Positives = 343/428 (80%)
 Frame = -2

Query: 1386 MLRHTLRRTLKSCTSSVWIQSRKSTIPSQSLLLETIPKENVDCXXXXXXXXXXXXARDLA 1207
            MLRH +R    S     WI S+  T  S+  +   + +E+VDC            AR+LA
Sbjct: 1    MLRHAIRGLRNSRR---WISSKNFT--SRVGVERAVARESVDCVVIGAGVVGIAVARELA 55

Query: 1206 LKGRDVLVIESAPTFGSGTSSRNSEVIHAGIYYPPNSLKAIFCLRGRKLLYQYCANHGVP 1027
            LKGR+V+VIE A TFG+GTSSRNSEVIHAGIYYP NSLKAIFC RGR+LLY+YC+ H VP
Sbjct: 56   LKGREVVVIEFASTFGTGTSSRNSEVIHAGIYYPRNSLKAIFCARGRELLYKYCSEHEVP 115

Query: 1026 HKQIGKLIVATGSSEIQKLIVLLSQGRENGVEGLRMMEGFEAMRMEPELKCLKALLSPYS 847
            HKQIGKLIVAT SSE+ KL  L+ +G ENGV+GLRM+EGFEAM MEPEL+C++ALLSP S
Sbjct: 116  HKQIGKLIVATRSSEVPKLNDLMIRGNENGVDGLRMLEGFEAMEMEPELQCVRALLSPAS 175

Query: 846  GIVDTHALMLSLVGEAENHGATFSYNTTVVGGHLEGDSIQIHISQSKNIEKCIGGCPPQP 667
            GIVDTH+LMLSLVGEAENH  TF YNT V+GGHLEGD I +HIS+SK++E      P  P
Sbjct: 176  GIVDTHSLMLSLVGEAENHRTTFCYNTAVIGGHLEGDHICLHISESKDLENWDRRFPLHP 235

Query: 666  ELILVPNLVVNSAGLSSPSLAKSFKGLDSRVIPPSYYAQGCYFTLSNNKTPPFEHLIYPI 487
            E+IL+  LVVNSAGLS+P+LAK F GL+S VIP S+YA+GCYF LS  KTPPF+HLIYPI
Sbjct: 236  EVILMAKLVVNSAGLSAPALAKRFDGLNSTVIPASHYARGCYFVLSKMKTPPFKHLIYPI 295

Query: 486  PEDGGLGVHVTLDLDGHVKFGPDVEWIGELDSISSFLNKFDYSVSSDRVQKFYPEIRRYY 307
            PEDGGLGVHVTLDLDG +KFGPDVEWI   D ISSFLNKFDYSV + RV++FYPEIR+YY
Sbjct: 296  PEDGGLGVHVTLDLDGQIKFGPDVEWINGADDISSFLNKFDYSVCAKRVERFYPEIRKYY 355

Query: 306  PNLKEGSLEPGYAGIRPKLSGPGQPPVDFIIQGEDINGVRGLINLFGIESPGLTSSLAIA 127
            PNLK+ SLEPGYAGIRPKLSG  Q  VDF+IQGEDI+GV GL+NLFGIESPGLTSS+AIA
Sbjct: 356  PNLKDDSLEPGYAGIRPKLSGAQQSAVDFVIQGEDIHGVPGLVNLFGIESPGLTSSMAIA 415

Query: 126  EHIGLKFI 103
            EHI  +++
Sbjct: 416  EHIVARYL 423


>ref|XP_002308844.1| predicted protein [Populus trichocarpa] gi|222854820|gb|EEE92367.1|
            predicted protein [Populus trichocarpa]
          Length = 430

 Score =  578 bits (1489), Expect = e-162
 Identities = 283/426 (66%), Positives = 350/426 (82%), Gaps = 3/426 (0%)
 Frame = -2

Query: 1371 LRRTLKSCTSSVWIQSRKSTIPSQSLLL--ETIPKENVDCXXXXXXXXXXXXARDLALKG 1198
            L++TL+S   +  I S+   I + S  +  E +P E +DC            AR+LA KG
Sbjct: 5    LKQTLQSLKRTATI-SKAQAIKNLSTQIKKEDVPTEKLDCVVIGAGVVGIAIARELASKG 63

Query: 1197 RDVLVIESAPTFGSGTSSRNSEVIHAGIYYPPNSLKAIFCLRGRKLLYQYCANHGVPHKQ 1018
            R+VLV++SA TFG+GTSSRNSEVIHAGIYYPP+SLKA+FC+RGR+LLY+YC  HGVP KQ
Sbjct: 64   REVLVLDSASTFGTGTSSRNSEVIHAGIYYPPDSLKALFCVRGRELLYRYCYEHGVPCKQ 123

Query: 1017 IGKLIVATGSSEIQKLIVLLSQGRENGVEGLRMMEGFEAMRMEPELKCLKALLSPYSGIV 838
            IGKLIVATG  EI KL  L+ +G +NGV+ L+M+EGFEAM+MEPEL+C KALLSP SGIV
Sbjct: 124  IGKLIVATGPLEISKLNELMDRGTQNGVDNLKMLEGFEAMKMEPELQCKKALLSPVSGIV 183

Query: 837  DTHALMLSLVGEAENHGATFSYNTTVVGGHLEGDSIQIHISQSKNIEKCIGGCPPQPELI 658
            D+H+LMLSLVGEAEN+G TFSYN+TV+ GHLEG+ + ++I +SK++E   G  P  PEL+
Sbjct: 184  DSHSLMLSLVGEAENNGTTFSYNSTVISGHLEGNCLHLYIVESKDLENWNGDYPLHPELV 243

Query: 657  LVPNLVVNSAGLSSPSLAKSFKGLDSRVIPPSYYAQGCYFTLSNNKTPPFEHLIYPIPED 478
            LVP LVVNSAGLSS +LAK F GLD+ +IPP ++A+GCYFTLS+ K PPFEHLIYPIPED
Sbjct: 244  LVPKLVVNSAGLSSLALAKRFHGLDNGIIPPGFFARGCYFTLSSTKVPPFEHLIYPIPED 303

Query: 477  GGLGVHVTLDLDGHVKFGPDVEWIGELDSISSFLNKFDYSVSSDRVQKFYPEIRRYYPNL 298
            GGLGVHVTLDLDGH+KFGPDVEWI  +D +SSFLNK+DYSVS+ R ++FYPEIR+YYPNL
Sbjct: 304  GGLGVHVTLDLDGHLKFGPDVEWIDGIDDVSSFLNKYDYSVSASRAERFYPEIRKYYPNL 363

Query: 297  KEGSLEPGYAGIRPKLSGPGQPPVDFII-QGEDINGVRGLINLFGIESPGLTSSLAIAEH 121
            K+GSL+PGY+GIRPK+SGP Q P+DF+I QGEDI+GV GL+NLFGIESPGLT+S+AIAEH
Sbjct: 364  KDGSLQPGYSGIRPKISGPRQSPIDFVIQQGEDIHGVPGLVNLFGIESPGLTASMAIAEH 423

Query: 120  IGLKFI 103
            I  +F+
Sbjct: 424  IASRFL 429


>ref|XP_003618905.1| L-2-hydroxyglutarate dehydrogenase [Medicago truncatula]
            gi|355493920|gb|AES75123.1| L-2-hydroxyglutarate
            dehydrogenase [Medicago truncatula]
          Length = 483

 Score =  570 bits (1469), Expect = e-160
 Identities = 280/446 (62%), Positives = 350/446 (78%), Gaps = 25/446 (5%)
 Frame = -2

Query: 1365 RTLKSCTSSVWIQSRKSTIPSQSLLLETIPKENVDCXXXXXXXXXXXXARDLALKGRDVL 1186
            R ++S T +    +    +  ++    ++P+E VDC            AR LALKGR+V+
Sbjct: 38   RNIRSTTQNDTNTTTSYPVSRETTTSYSVPRERVDCVVIGAGVVGIAVARALALKGREVI 97

Query: 1185 VIESAPTFGSGTSSRNSEVIHAGIYYPPNSLKAIFCLRGRKLLYQYCANHGVPHKQIGKL 1006
            VIESAP+FG+GTSSRNSEV+HAGIYYP +SLKAIFC++GR++LY+YCA H +PH+Q GKL
Sbjct: 98   VIESAPSFGTGTSSRNSEVVHAGIYYPHHSLKAIFCVKGREMLYEYCAKHDIPHEQTGKL 157

Query: 1005 IVATGSSEIQKLIVLLSQGRENGVEGLRMMEGFEAMRMEPELKCLKALLSPYSGIVDTHA 826
            IVAT SSEI KL V+L+ G +NGV+GL+MM+G +AM+MEPEL+C+KA+LSP SGIVD+H+
Sbjct: 158  IVATRSSEIPKLSVILNHGIQNGVDGLKMMDGVDAMKMEPELQCVKAILSPLSGIVDSHS 217

Query: 825  LMLSLV-------------------------GEAENHGATFSYNTTVVGGHLEGDSIQIH 721
            LMLSLV                         GEAENHGATF+YN+TV+GGH+EG+ I +H
Sbjct: 218  LMLSLVVCISSFYYHVVKLDLVLNINPMLVQGEAENHGATFTYNSTVIGGHMEGNEICLH 277

Query: 720  ISQSKNIEKCIGGCPPQPELILVPNLVVNSAGLSSPSLAKSFKGLDSRVIPPSYYAQGCY 541
            +S++K++++  G    QPEL+L+P LVVNSAGLS+ +LAK F GL+++VIPP+YYA+GCY
Sbjct: 278  VSETKSLKEWNGKSSLQPELVLIPKLVVNSAGLSALALAKRFTGLENKVIPPAYYARGCY 337

Query: 540  FTLSNNKTPPFEHLIYPIPEDGGLGVHVTLDLDGHVKFGPDVEWIGELDSISSFLNKFDY 361
            FTLSN K  PF HLIYPIPEDGGLGVHVTLDL+G VKFGPDVEWI  +D ISSF NKFDY
Sbjct: 338  FTLSNTKASPFRHLIYPIPEDGGLGVHVTLDLNGQVKFGPDVEWIDGVDDISSFQNKFDY 397

Query: 360  SVSSDRVQKFYPEIRRYYPNLKEGSLEPGYAGIRPKLSGPGQPPVDFIIQGEDINGVRGL 181
            SV ++R +KFYPEIR+YYPNLK+GSLEPGY+GIRPKLSGP Q PVDF+IQGEDI+GV GL
Sbjct: 398  SVQANRAEKFYPEIRKYYPNLKDGSLEPGYSGIRPKLSGPCQSPVDFVIQGEDIHGVPGL 457

Query: 180  INLFGIESPGLTSSLAIAEHIGLKFI 103
            INLFGIESPGLTSSLAIA+ I  KF+
Sbjct: 458  INLFGIESPGLTSSLAIADFISTKFL 483


>ref|XP_003525735.1| PREDICTED: l-2-hydroxyglutarate dehydrogenase, mitochondrial-like
            [Glycine max]
          Length = 410

 Score =  561 bits (1446), Expect = e-157
 Identities = 268/395 (67%), Positives = 330/395 (83%)
 Frame = -2

Query: 1287 ETIPKENVDCXXXXXXXXXXXXARDLALKGRDVLVIESAPTFGSGTSSRNSEVIHAGIYY 1108
            +++PKE V+C            AR LALKGR+VLV+ESAPTFG+GTSSRNSEVIHAGIYY
Sbjct: 16   DSVPKERVECVVIGAGVVGIAVARALALKGREVLVVESAPTFGTGTSSRNSEVIHAGIYY 75

Query: 1107 PPNSLKAIFCLRGRKLLYQYCANHGVPHKQIGKLIVATGSSEIQKLIVLLSQGRENGVEG 928
            P NS KAIFC+RGR++LY+YC+ H +PHKQIGKLIVAT SSEI KL  +L+ G +NGV+G
Sbjct: 76   PLNSFKAIFCVRGREMLYEYCSKHDIPHKQIGKLIVATRSSEIPKLNDILNCGIQNGVDG 135

Query: 927  LRMMEGFEAMRMEPELKCLKALLSPYSGIVDTHALMLSLVGEAENHGATFSYNTTVVGGH 748
            L++++G EAM+MEPEL+C+KA+LSP +GIVD+H+LMLSLVGEAEN G TF+YN+TV+GGH
Sbjct: 136  LKIVDGVEAMKMEPELQCVKAILSPLTGIVDSHSLMLSLVGEAENQGTTFTYNSTVIGGH 195

Query: 747  LEGDSIQIHISQSKNIEKCIGGCPPQPELILVPNLVVNSAGLSSPSLAKSFKGLDSRVIP 568
            LEG  I +H++++  + +  G    QPEL+L+P LVVNS GLSSP LAK F G  S V+P
Sbjct: 196  LEGSEICLHVTETDRLTEWKGTSILQPELLLIPKLVVNSTGLSSPVLAKRFNGPKSGVVP 255

Query: 567  PSYYAQGCYFTLSNNKTPPFEHLIYPIPEDGGLGVHVTLDLDGHVKFGPDVEWIGELDSI 388
            P+YYA+GCYFTLSN K  PF  LIYPIPEDGG+GVHVT+DL+G VKFGP+VEWI  +D I
Sbjct: 256  PAYYARGCYFTLSNTKNSPFRRLIYPIPEDGGIGVHVTIDLNGQVKFGPNVEWIDSVDDI 315

Query: 387  SSFLNKFDYSVSSDRVQKFYPEIRRYYPNLKEGSLEPGYAGIRPKLSGPGQPPVDFIIQG 208
            SSF NK+DYSV+++R ++FYPEIR+YYPNLK+GSLEPGY+GIRPKLSGP QPP DF+IQG
Sbjct: 316  SSFQNKYDYSVNANRAERFYPEIRKYYPNLKDGSLEPGYSGIRPKLSGPLQPPSDFVIQG 375

Query: 207  EDINGVRGLINLFGIESPGLTSSLAIAEHIGLKFI 103
            EDI+GV GLINLFGIESPGLTSS+AIAE I  +F+
Sbjct: 376  EDIHGVPGLINLFGIESPGLTSSMAIAEFISTRFL 410


>gb|AFK34722.1| unknown [Lotus japonicus]
          Length = 418

 Score =  551 bits (1421), Expect = e-154
 Identities = 266/398 (66%), Positives = 326/398 (81%)
 Frame = -2

Query: 1293 LLETIPKENVDCXXXXXXXXXXXXARDLALKGRDVLVIESAPTFGSGTSSRNSEVIHAGI 1114
            +++++P+E VDC            AR LALKGR+VLVIES  TFG+GTSSRNSEVIHAGI
Sbjct: 21   IMDSVPREKVDCVVIGAGVVGIAVARALALKGREVLVIESGSTFGTGTSSRNSEVIHAGI 80

Query: 1113 YYPPNSLKAIFCLRGRKLLYQYCANHGVPHKQIGKLIVATGSSEIQKLIVLLSQGRENGV 934
            YYP +SLKAIFC+RGR++LY+YC+ H +PHKQ+GKLIVAT SSEI KL  +L+ G +NGV
Sbjct: 81   YYPRDSLKAIFCVRGREMLYEYCSMHDIPHKQVGKLIVATRSSEIPKLNDILNHGIQNGV 140

Query: 933  EGLRMMEGFEAMRMEPELKCLKALLSPYSGIVDTHALMLSLVGEAENHGATFSYNTTVVG 754
            + L M+ G +AM+MEPEL+C+KA+LSP SGIVD+H+LMLSLVGEAENH  TF+YN+TV+G
Sbjct: 141  DSLEMINGAKAMKMEPELQCVKAILSPLSGIVDSHSLMLSLVGEAENHRTTFTYNSTVIG 200

Query: 753  GHLEGDSIQIHISQSKNIEKCIGGCPPQPELILVPNLVVNSAGLSSPSLAKSFKGLDSRV 574
            GHLEG  I +HI ++K +++  G     PELILVP L+VNS GLS+P+LAK F  L S V
Sbjct: 201  GHLEGKEICLHILETKCLKEWDGTSILHPELILVPKLLVNSTGLSAPALAKRFTSLQSEV 260

Query: 573  IPPSYYAQGCYFTLSNNKTPPFEHLIYPIPEDGGLGVHVTLDLDGHVKFGPDVEWIGELD 394
            IPPSYYA+GCYFTLS+ K  PF HLIYPIPEDGGLGVHVTLDL+G +KFGPDVEWI  +D
Sbjct: 261  IPPSYYARGCYFTLSDTKASPFRHLIYPIPEDGGLGVHVTLDLNGQIKFGPDVEWIDGVD 320

Query: 393  SISSFLNKFDYSVSSDRVQKFYPEIRRYYPNLKEGSLEPGYAGIRPKLSGPGQPPVDFII 214
             ISSF NKFDYSV ++R ++FYP IR YYPNLK+ +LEPGYAGIRPKLS PGQ PVDF+I
Sbjct: 321  DISSFQNKFDYSVHANRAEQFYPAIRSYYPNLKDRALEPGYAGIRPKLSRPGQSPVDFVI 380

Query: 213  QGEDINGVRGLINLFGIESPGLTSSLAIAEHIGLKFIG 100
            QGEDI+G+ GL+NLFGIESPGLT+SLAIA+ +  + +G
Sbjct: 381  QGEDIHGIPGLVNLFGIESPGLTASLAIADFVSTRLLG 418