BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00017842
         (810 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277900.1| PREDICTED: probable steroid reductase DET2 [...   402   e-110
emb|CAN63063.1| hypothetical protein VITISV_021171 [Vitis vinifera]   402   e-110
ref|XP_002323554.1| predicted protein [Populus trichocarpa] gi|2...   368   e-99 
ref|XP_002527863.1| 3-oxo-5-alpha-steroid 4-dehydrogenase, putat...   357   2e-96
sp|Q2QDF6.1|DET2_GOSHI RecName: Full=Steroid 5-alpha-reductase D...   356   4e-96

>ref|XP_002277900.1| PREDICTED: probable steroid reductase DET2 [Vitis vinifera]
           gi|297740252|emb|CBI30434.3| unnamed protein product
           [Vitis vinifera]
          Length = 257

 Score =  402 bits (1033), Expect = e-110
 Identities = 186/256 (72%), Positives = 213/256 (83%), Gaps = 1/256 (0%)
 Frame = +3

Query: 21  DESLFHYSLLTFFIITPPTFISLRFLEAPYGKHYRKGWGPTISPSLAWFLMESPTIWLTL 200
           D +LFH SL+  ++I PPTFISLRFL+APYGKH+R GWGPT+ PSLAWFLMESPT+WLTL
Sbjct: 4   DRTLFHNSLIALYLIAPPTFISLRFLQAPYGKHHRSGWGPTVPPSLAWFLMESPTVWLTL 63

Query: 201 LIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRLRNTNPKKTTKASFPVSVALMGFGF 380
           LI+P G   SN +S+ LISI+LFHYIHRT+IYPL LRN+   K  +  FPVSVALM FGF
Sbjct: 64  LIYPLGDRSSNPRSLILISIYLFHYIHRTLIYPLHLRNSTAAK--RNGFPVSVALMAFGF 121

Query: 381 NILNAYLQSRWVSHYYVAENDWWFGVRFVIGLVIFLSGMIVNVRSDLILVKLKGEDGGYK 560
           N+LNAYLQ+RWVSHY   E+D WF  RF +GLVIFL GM+VNVRSDL LV LK + GGYK
Sbjct: 122 NLLNAYLQARWVSHYANYEDDGWFWWRFWVGLVIFLDGMLVNVRSDLALVGLKSQGGGYK 181

Query: 561 IPRGGWFERVSCANYFGEIVEWLGWAVMTWSWVGFAFFIYTCANLVPRARANHIWYRDKF 740
           +PRGGWFE VSCANYFGE+VEWLGWAVMTWSWVG  F +YTCANLVPRARANH WY +KF
Sbjct: 182 VPRGGWFELVSCANYFGEVVEWLGWAVMTWSWVGLGFLLYTCANLVPRARANHKWYLEKF 241

Query: 741 -KEYPTSRKAVIPFLY 785
            ++YP  RKAVIPFLY
Sbjct: 242 GEDYPKGRKAVIPFLY 257


>emb|CAN63063.1| hypothetical protein VITISV_021171 [Vitis vinifera]
          Length = 257

 Score =  402 bits (1032), Expect = e-110
 Identities = 186/256 (72%), Positives = 213/256 (83%), Gaps = 1/256 (0%)
 Frame = +3

Query: 21  DESLFHYSLLTFFIITPPTFISLRFLEAPYGKHYRKGWGPTISPSLAWFLMESPTIWLTL 200
           D +LFH SL+  ++I PPTFISLRFL+APYGKH+R GWGPT+ PSLAWFLMESPT+WLTL
Sbjct: 4   DRTLFHNSLIALYLIAPPTFISLRFLQAPYGKHHRXGWGPTVPPSLAWFLMESPTVWLTL 63

Query: 201 LIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRLRNTNPKKTTKASFPVSVALMGFGF 380
           LI+P G   SN +S+ LISI+LFHYIHRT+IYPL LRN+   K  +  FPVSVALM FGF
Sbjct: 64  LIYPLGDRSSNPRSLILISIYLFHYIHRTLIYPLHLRNSTAAK--RNGFPVSVALMAFGF 121

Query: 381 NILNAYLQSRWVSHYYVAENDWWFGVRFVIGLVIFLSGMIVNVRSDLILVKLKGEDGGYK 560
           N+LNAYLQ+RWVSHY   E+D WF  RF +GLVIFL GM+VNVRSDL LV LK + GGYK
Sbjct: 122 NLLNAYLQARWVSHYANYEDDGWFWWRFWVGLVIFLDGMLVNVRSDLALVGLKSQGGGYK 181

Query: 561 IPRGGWFERVSCANYFGEIVEWLGWAVMTWSWVGFAFFIYTCANLVPRARANHIWYRDKF 740
           +PRGGWFE VSCANYFGE+VEWLGWAVMTWSWVG  F +YTCANLVPRARANH WY +KF
Sbjct: 182 VPRGGWFELVSCANYFGEVVEWLGWAVMTWSWVGLGFLLYTCANLVPRARANHKWYLEKF 241

Query: 741 -KEYPTSRKAVIPFLY 785
            ++YP  RKAVIPFLY
Sbjct: 242 GEDYPKGRKAVIPFLY 257


>ref|XP_002323554.1| predicted protein [Populus trichocarpa] gi|222868184|gb|EEF05315.1|
           predicted protein [Populus trichocarpa]
          Length = 257

 Score =  368 bits (944), Expect = e-99
 Identities = 179/262 (68%), Positives = 208/262 (79%), Gaps = 4/262 (1%)
 Frame = +3

Query: 9   MKLSDESLFHYSLLTFFIITPPTFISLRFLEAPYGKHYRKGWGPTISPSLAWFLMESPTI 188
           M L D+SLFHYSLL+ ++I PPTFISL+FL+APYGKH+R GWGPTISP LAW LMESPT+
Sbjct: 1   MALLDQSLFHYSLLSIYLIGPPTFISLKFLQAPYGKHHRPGWGPTISPPLAWILMESPTL 60

Query: 189 WLTLLIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRLRNTNPKKTTKASFPVSVALM 368
           WLTLL+FP+GQ+ +N K+I L+S FL HY HRT IYPLR+   +  KTT   FPVSVA+M
Sbjct: 61  WLTLLLFPYGQHFTNPKAIILMSPFLLHYFHRTCIYPLRIYRNS--KTT-GGFPVSVAVM 117

Query: 369 GFGFNILNAYLQSRWVSHY---YVAENDWWFGVRFVIGLVIFLSGMIVNVRSDLILVKLK 539
            FGFN+LNAYLQ+RWVSHY   Y     WW   +F  GLV+F+ GM +N  +D +L+ LK
Sbjct: 118 AFGFNLLNAYLQARWVSHYKSDYDGGLFWW---KFFGGLVVFVWGMRINTWADGVLLGLK 174

Query: 540 GEDGGYKIPRGGWFERVSCANYFGEIVEWLGWAVMTWSWVGFAFFIYTCANLVPRARANH 719
            E GGYK+PRGGWFE VSC NYFGEIVEWLGWAVMTWSW GF F +YTCANLVPRA ANH
Sbjct: 175 REGGGYKVPRGGWFELVSCPNYFGEIVEWLGWAVMTWSWAGFGFLLYTCANLVPRACANH 234

Query: 720 IWYRDKFKE-YPTSRKAVIPFL 782
            WY +KFKE YP SRKAVIPFL
Sbjct: 235 KWYLEKFKEDYPRSRKAVIPFL 256


>ref|XP_002527863.1| 3-oxo-5-alpha-steroid 4-dehydrogenase, putative [Ricinus communis]
           gi|223532714|gb|EEF34494.1| 3-oxo-5-alpha-steroid
           4-dehydrogenase, putative [Ricinus communis]
          Length = 263

 Score =  357 bits (916), Expect = 2e-96
 Identities = 178/263 (67%), Positives = 202/263 (76%), Gaps = 4/263 (1%)
 Frame = +3

Query: 9   MKLSDESLFHYSLLTFFIITPPTFISLRFLEAPYGKHYRKGWGPTISPSLAWFLMESPTI 188
           M  SD SLFH SLL  ++I PPTFISL+FL+APYGKH R GWGPTISP LAWFLMESPT+
Sbjct: 1   MAFSDGSLFHKSLLALYLIGPPTFISLKFLQAPYGKHNRPGWGPTISPPLAWFLMESPTL 60

Query: 189 WLTLLIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRLR-NTNPKKTTKAS-FPVSVA 362
           WLT L+FPFGQ+ SN K+  L+  +LFHY HRT +YPLRL  NTN + T   S FPVS+A
Sbjct: 61  WLTFLLFPFGQHASNPKAWVLMFPYLFHYFHRTCLYPLRLYWNTNQRNTKITSGFPVSMA 120

Query: 363 LMGFGFNILNAYLQSRWVSHYYV-AENDWWFGVRFVIGLVIFLSGMIVNVRSDLILVKLK 539
           L+ F FN LNAYLQ+RWVSHY    + D WF  RF  GLV+F  GM  NV SD +LV+LK
Sbjct: 121 LLAFLFNFLNAYLQARWVSHYKEDYDQDRWFWWRFWGGLVVFAWGMWTNVWSDRVLVRLK 180

Query: 540 GEDGGYKIPRGGWFERVSCANYFGEIVEWLGWAVMTWSWVGFAFFIYTCANLVPRARANH 719
            E  GYK+PRGG F+ VSC NYFGEIVEWLGW +MTWSWVGF FF+YT ANLVPRA ANH
Sbjct: 181 REGDGYKVPRGGLFDLVSCPNYFGEIVEWLGWTLMTWSWVGFGFFLYTSANLVPRAVANH 240

Query: 720 IWYRDKF-KEYPTSRKAVIPFLY 785
            WY DKF K+YP  RKAVIPFLY
Sbjct: 241 KWYLDKFGKDYPRGRKAVIPFLY 263


>sp|Q2QDF6.1|DET2_GOSHI RecName: Full=Steroid 5-alpha-reductase DET2; Short=GhDET2
           gi|73761693|gb|AAZ83346.1| steroid 5-alpha-reductase
           [Gossypium hirsutum]
          Length = 258

 Score =  356 bits (913), Expect = 4e-96
 Identities = 177/260 (68%), Positives = 204/260 (78%), Gaps = 4/260 (1%)
 Frame = +3

Query: 18  SDESLFHYSLLTFFIITPPTFISLRFLEAPYGKHYRKGWGPTISPSLAWFLMESPTIWLT 197
           SD++LFHY LLT +II  PT+ISL FL+APYGKH R GWGPTISP LAWFLMESPT+WLT
Sbjct: 3   SDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLWLT 62

Query: 198 LLIFPFGQNKSNFKSISLISIFLFHYIHRTIIYPLRL-RNTNPKKTTKASFPVSVALMGF 374
             +FP GQ+  N KS  LIS FLFHY +RT++YPLRL RNT    T    FPVSVA M F
Sbjct: 63  FFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRLARNT----TQTRGFPVSVAFMAF 118

Query: 375 GFNILNAYLQSRWVSHYY-VAENDWWFGVRFVIGLVIFLSGMIVNVRSDLILVKLKGE-D 548
           GFN+LN YLQ+RWVSHY    EN+  F  RF+ GL+IF+ GM VNVR+D +LV LK + D
Sbjct: 119 GFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKKQGD 178

Query: 549 GGYKIPRGGWFERVSCANYFGEIVEWLGWAVMTWSWVGFAFFIYTCANLVPRARANHIWY 728
           GGYKIPRGG FE VSC NYFGEI+EW GWAVMTWSWVGF FF+YTCANL+PRARA  +WY
Sbjct: 179 GGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATRLWY 238

Query: 729 RDKFK-EYPTSRKAVIPFLY 785
            +KFK +YP  RKAVIPF+Y
Sbjct: 239 LEKFKDDYPKDRKAVIPFIY 258