BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00017525
         (430 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285453.1| PREDICTED: probable 3-beta-hydroxysteroid-De...   177   8e-43
gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]   171   4e-41
ref|XP_002304628.1| predicted protein [Populus trichocarpa] gi|2...   166   2e-39
gb|ABU95101.1| putative C-8,7 sterol isomerase [Solanum tuberosum]    164   5e-39
ref|XP_002512695.1| sterol isomerase, putative [Ricinus communis...   161   4e-38

>ref|XP_002285453.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 2 [Vitis vinifera]
           gi|225435456|ref|XP_002285450.1| PREDICTED: probable
           3-beta-hydroxysteroid-Delta(8),Delta(7)-isomerase
           isoform 1 [Vitis vinifera] gi|297746323|emb|CBI16379.3|
           unnamed protein product [Vitis vinifera]
          Length = 222

 Score =  177 bits (449), Expect = 8e-43
 Identities = 94/154 (61%), Positives = 111/154 (72%), Gaps = 11/154 (7%)
 Frame = -1

Query: 430 KLSKIERLLM*WWAFTGLTHVILECYFAFSPQFYKDKSAHFLAEVWKGYRKGDSRYAGRD 251
           K SKI+RLLM WWAFTGLTH ILE YF FSP+FYKDK+A +LAEVWK Y KGDSRYA RD
Sbjct: 54  KTSKIDRLLMCWWAFTGLTHAILEGYFVFSPEFYKDKTACYLAEVWKEYSKGDSRYAARD 113

Query: 250 SGVVSVEGITAVIE-----------EWQVC*NFIQGCPSDFGQLYGTAVYFITSFLEGDN 104
           +GV++VEGITAV+E             +   ++I       GQLYGTAVYFITS+L+GDN
Sbjct: 114 AGVIAVEGITAVLEGPASLLALYTIATKRSYSYILQLTISLGQLYGTAVYFITSYLDGDN 173

Query: 103 FASSPXXXXXXXYVFMNSFWVWIPALIVIRSWKR 2
           FA+S        YVF N+FWV IP+LIVIR WK+
Sbjct: 174 FAAS-AYYYYAYYVFANAFWVVIPSLIVIRCWKK 206


>gb|ADG02944.1| putative C-8,7 sterol isomerase [Gossypium hirsutum]
          Length = 217

 Score =  171 bits (434), Expect = 4e-41
 Identities = 90/154 (58%), Positives = 110/154 (71%), Gaps = 12/154 (7%)
 Frame = -1

Query: 427 LSKIERLLM*WWAFTGLTHVILECYFAFSPQFYKDKSAHFLAEVWKGYRKGDSRYAGRDS 248
           +SK++RLLM WWAFTGLTH+ILE YFAFSP+FYKDK+  +LAEVWK Y KGDSRYAGRDS
Sbjct: 50  ISKLDRLLMCWWAFTGLTHIILEGYFAFSPEFYKDKTGFYLAEVWKEYSKGDSRYAGRDS 109

Query: 247 GVVSVEGITAVIEEWQVC------------*NFIQGCPSDFGQLYGTAVYFITSFLEGDN 104
            +V+VEGIT+V+ E   C             ++I       GQLYGT VYFIT++LEGDN
Sbjct: 110 TIVAVEGITSVL-EGPPCLLAVYAIAKGKGYSYILQFAISLGQLYGTFVYFITAYLEGDN 168

Query: 103 FASSPXXXXXXXYVFMNSFWVWIPALIVIRSWKR 2
           F++SP       YV  NSFW+ IP+LI IR WK+
Sbjct: 169 FSASP-FYYYAYYVLANSFWLLIPSLIAIRCWKK 201


>ref|XP_002304628.1| predicted protein [Populus trichocarpa] gi|222842060|gb|EEE79607.1|
           predicted protein [Populus trichocarpa]
          Length = 220

 Score =  166 bits (419), Expect = 2e-39
 Identities = 87/164 (53%), Positives = 110/164 (67%), Gaps = 21/164 (12%)
 Frame = -1

Query: 430 KLSKIERLLM*WWAFTGLTHVILECYFAFSPQFYKDKSAHFLAEVWKGYRKGDSRYAGRD 251
           K++K+ RLLM WWAFTGLTH+ILE YFAFSP+FYKDK+AH+LAEVWK Y KGDSRYA RD
Sbjct: 52  KITKMNRLLMCWWAFTGLTHLILEGYFAFSPEFYKDKTAHYLAEVWKEYSKGDSRYAARD 111

Query: 250 SGVVSVEGITAVIE---------------------EWQVC*NFIQGCPSDFGQLYGTAVY 134
           +  V+VEG+TAV+E                     ++ VC           GQLYGTAVY
Sbjct: 112 AATVTVEGLTAVLEGPASLLAVYAIASGKSYSYILQFAVC----------LGQLYGTAVY 161

Query: 133 FITSFLEGDNFASSPXXXXXXXYVFMNSFWVWIPALIVIRSWKR 2
           F+T++LEGD+FA+SP       Y+  N+ WV IP+LI +R WK+
Sbjct: 162 FLTAYLEGDHFATSP-YHYYVYYIGANASWVVIPSLIAMRCWKK 204


>gb|ABU95101.1| putative C-8,7 sterol isomerase [Solanum tuberosum]
          Length = 221

 Score =  164 bits (416), Expect = 5e-39
 Identities = 86/155 (55%), Positives = 104/155 (67%), Gaps = 12/155 (7%)
 Frame = -1

Query: 430 KLSKIERLLM*WWAFTGLTHVILECYFAFSPQFYKDKSAHFLAEVWKGYRKGDSRYAGRD 251
           KLSK +R+LM WW FTGLTH++LE YF F+P FY+  S  +LAEVWK Y KGDSRY GRD
Sbjct: 54  KLSKTDRVLMCWWIFTGLTHMVLEGYFVFTPDFYQKTSPVYLAEVWKEYSKGDSRYVGRD 113

Query: 250 SGVVSVEGITAVIEEWQVC*NFIQGCPSD------------FGQLYGTAVYFITSFLEGD 107
           + V+SVEG+TAVI E   C   +    +              GQLYGTA+YFIT+ LEGD
Sbjct: 114 AAVLSVEGVTAVI-EGPACLLAVYAIATKKAYRHVLQLSICLGQLYGTAIYFITAVLEGD 172

Query: 106 NFASSPXXXXXXXYVFMNSFWVWIPALIVIRSWKR 2
           NF++SP       YVF N FWVWIP+LIVI  WK+
Sbjct: 173 NFSTSP-FYYYAYYVFANHFWVWIPSLIVIHCWKK 206


>ref|XP_002512695.1| sterol isomerase, putative [Ricinus communis]
           gi|223548656|gb|EEF50147.1| sterol isomerase, putative
           [Ricinus communis]
          Length = 220

 Score =  161 bits (408), Expect = 4e-38
 Identities = 86/154 (55%), Positives = 106/154 (68%), Gaps = 11/154 (7%)
 Frame = -1

Query: 430 KLSKIERLLM*WWAFTGLTHVILECYFAFSPQFYKDKSAHFLAEVWKGYRKGDSRYAGRD 251
           K +KI+R LM WWAFTGLTH+ILE YF FSP+FYKDK++ +LAEVWK Y KGDSRYA RD
Sbjct: 52  KAAKIDRWLMCWWAFTGLTHIILEGYFVFSPEFYKDKTSFYLAEVWKEYSKGDSRYAARD 111

Query: 250 SGVVSVEGITAVIE-----------EWQVC*NFIQGCPSDFGQLYGTAVYFITSFLEGDN 104
           S VV+VEG+TAV+E                 ++I       GQLYGTAVYF+T +L+G N
Sbjct: 112 SAVVAVEGLTAVLEGPASLLAVYAIAAGKSYSYILQFAISLGQLYGTAVYFLTPYLDGYN 171

Query: 103 FASSPXXXXXXXYVFMNSFWVWIPALIVIRSWKR 2
           +ASSP       Y+  N+ WV IP+LI IRSWK+
Sbjct: 172 YASSP-YYHYAYYIGANASWVVIPSLICIRSWKK 204