BLASTX nr result
ID: Coptis24_contig00016661
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00016661
(1402 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_194638.1| Ribonuclease H-like protein [Arabidopsis thalia... 91 9e-16
ref|NP_187562.1| RNase H domain-containing protein [Arabidopsis ... 87 8e-15
emb|CCA66036.1| hypothetical protein [Beta vulgaris subsp. vulga... 87 8e-15
gb|AAD24831.1| putative non-LTR retroelement reverse transcripta... 87 1e-14
gb|EEE66057.1| hypothetical protein OsJ_22054 [Oryza sativa Japo... 79 2e-14
>ref|NP_194638.1| Ribonuclease H-like protein [Arabidopsis thaliana]
gi|4972055|emb|CAB43923.1| putative protein [Arabidopsis
thaliana] gi|7269807|emb|CAB79667.1| putative protein
[Arabidopsis thaliana] gi|67633766|gb|AAY78807.1|
putative reverse transcriptase/RNA-dependent DNA
polymerase [Arabidopsis thaliana]
gi|332660185|gb|AEE85585.1| Ribonuclease H-like protein
[Arabidopsis thaliana]
Length = 575
Score = 90.5 bits (223), Expect = 9e-16
Identities = 93/388 (23%), Positives = 154/388 (39%), Gaps = 11/388 (2%)
Frame = +3
Query: 129 LKVNSLFSPVLLSWNQNRLGELFPFXXXXXXXXXXXXPSGSPDHIIWPATKNRELTVKSA 308
LKV+ L W ++ + LFP D W T + + TVKS
Sbjct: 169 LKVSDLIDESGREWRKDVIEMLFPEVERKLIGELRPGGRRILDSYTWDYTSSGDYTVKSG 228
Query: 309 YVFLTKQDNISFSS------SFNPLSMKNLWKLHLDAAT*LFIWKLYIGGLPTGDVLHKF 470
Y LT+ N S S NP+ K +WK F+WK LP L
Sbjct: 229 YWVLTQIINKRSSPQEVSEPSLNPIYQK-IWKSQTSPKIQHFLWKCLSNSLPVAGALAYR 287
Query: 471 KFKGDISCSFCQKCIETASHVFFSCTWIKMMWYVSNAMLDINVHPDWNVKEWINQF-IFW 647
+ +C C C ET +H+ F CT+ ++ W +S+ + I + +W ++N + +F
Sbjct: 288 HLSKESACIRCPSCKETVNHLLFKCTFARLTWAISS--IPIPLGGEWADSIYVNLYWVFN 345
Query: 648 CSSKDEDICRRGYLCLFILYELWLARNKARMECRPIELKSILNFSDMKRE---ITSLAFL 818
+ + + L ++L+ LW RN+ R + +L ++ E I + A
Sbjct: 346 LGNGNPQWEKASQLVPWLLWRLWKNRNELVFRGREFNAQEVLRRAEDDLEEWRIRTEAES 405
Query: 819 SITYPGLPLSFNIIXXXXXXXXXVKVNFNVAFDRSHFSAAVGVVVRNAEGVILGAAFRRF 998
T P + S + VK N + ++R + +G V+RN +G + R
Sbjct: 406 CGTKPQVNRS-SCGRWRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARAL 464
Query: 999 -SANDPEQAELIGAEVAILLALRLNLCFVILEGDCQTLMSALKTCNSSLLG*NSFFVFQH 1175
+AEL A+L R +VI E D Q L+ L N+ + + Q
Sbjct: 465 PKLKSVLEAELEAMRWAVLSLSRFQYNYVIFESDSQVLIEIL---NNDEIWPSLKPTIQD 521
Query: 1176 IFALAAGLDKFVFSWVSRTGNGFAHGLA 1259
+ L + + F ++ R GN A +A
Sbjct: 522 LQRLLSQFTEVKFVFIPREGNTLAERVA 549
>ref|NP_187562.1| RNase H domain-containing protein [Arabidopsis thaliana]
gi|6682231|gb|AAF23283.1|AC016661_8 putative non-LTR
reverse transcriptase [Arabidopsis thaliana]
gi|332641254|gb|AEE74775.1| RNase H domain-containing
protein [Arabidopsis thaliana]
Length = 484
Score = 87.4 bits (215), Expect = 8e-15
Identities = 90/395 (22%), Positives = 162/395 (41%), Gaps = 15/395 (3%)
Frame = +3
Query: 126 DLKVNSLFSPV--LLSWNQNRLGELFPFXXXXXXXXXXXXPSGSPDHIIWPATKNRELTV 299
++ +N+LF W+ +++ + S PD IIW E TV
Sbjct: 72 EMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKIIWNYNTTGEYTV 131
Query: 300 KSAYVFLTKQDNISFSSSFNP---LSMKN-LWKLHLDAAT*LFIWKLYIGGLPTGDVLHK 467
+S Y LT + + + P + +K +W L + F+W+ L T + L
Sbjct: 132 RSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRALSQALATTERLTT 191
Query: 468 FKFKGDISCSFCQKCIETASHVFFSCTWIKMMWYVSNAMLDINVHPDWNVKEWINQFIFW 647
+ D SC C + E+ +H F+C + M W +S++ L N + +E I+ + +
Sbjct: 192 RGMRIDPSCPRCHRENESINHALFTCPFATMAWRLSDSSLIRNQLMSNDFEENISNILNF 251
Query: 648 CSSKDEDICRRGYLCLFILYELWLARNKARM-ECRPIELKSILNFSDMKRE--ITSLAFL 818
+ L +++++ +W ARN + R K++L+ + + +
Sbjct: 252 VQDTTMSDFHK-LLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNATQSHK 310
Query: 819 SITYPGLPLSFNIIXXXXXXXXXVKVNFNVAFDRSHFSAAVGVVVRNAEGV-ILGAAFRR 995
P ++ N I VK NF+ FD A G ++RN G I + +
Sbjct: 311 KTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKL 370
Query: 996 FSANDPEQAELIGAEVAILLALRLNLCFVILEGDCQTLMSALK--TCNSSLLG*NSFFVF 1169
++P +AE A+ V +EGDCQTL++ + + +SSL
Sbjct: 371 AHTSNPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLINGISFHSSLA-------- 422
Query: 1170 QHIFALAAGLDKFV---FSWVSRTGNGFAHGLASW 1265
H+ ++ +KF F ++ R GN AH LA +
Sbjct: 423 NHLEDISFWANKFASIQFGFIRRKGNKLAHVLAKY 457
>emb|CCA66036.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
Length = 1369
Score = 87.4 bits (215), Expect = 8e-15
Identities = 93/380 (24%), Positives = 148/380 (38%), Gaps = 6/380 (1%)
Frame = +3
Query: 168 WNQNRLGELFPFXXXXXXXXXXXXPSGSPDHIIWPATKNRELTVKSAYVFLTKQDNISFS 347
WN L LF PD +W +KN + TV+SAY +D +
Sbjct: 981 WNVELLNTLFQPWESTAIQRIPVALQKKPDQWMWMMSKNGQFTVRSAYYHELLEDRKTGP 1040
Query: 348 SSFNPLSMK---NLWKLHLDAAT*LFIWKLYIGGLPTGDVLHKFKFKGDISCSFCQKCIE 518
S+ ++K +WK + LF WK GL + K D +C C + E
Sbjct: 1041 STSRGPNLKLWQKIWKAKIPPKVKLFSWKAIHNGLAVYTNMRKRGMNIDGACPRCGEKEE 1100
Query: 519 TASHVFFSCTWIKMMWYVSNAMLDINVHPDWNVKEWINQFIFWCSSKDEDICRRGYLCLF 698
T H+ + C WY+S + +H N++ F W S + + LF
Sbjct: 1101 TTEHLIWGCDESSRAWYIS----PLRIHTG-NIE--AGSFRIWVESLLDTHKDTEWWALF 1153
Query: 699 --ILYELWLARNKARMECRPIELKSILNFSDMKREITSLAFLSITYPGLPLSFNIIXXXX 872
I + +WL RNK E + + + ++ + ++ + + T P L+ +
Sbjct: 1154 WMICWNIWLGRNKWVFEKKKLAFQEVVERA-VRGVMEFEEECAHTSPVETLNTHENGWSV 1212
Query: 873 XXXXXVKVNFNVAFDRSHFSAAVGVVVRNAEG-VILGAAFRRFSANDPEQAELIGAEVAI 1049
VK+N + A + H +G VVR+AEG V+L ++ DP AE +
Sbjct: 1213 PPVGMVKLNVDAAVFK-HVGIGMGGVVRDAEGDVLLATCCGGWAMEDPAMAEACSLRYGL 1271
Query: 1050 LLALRLNLCFVILEGDCQTLMSALKTCNSSLLG*NSFFVFQHIFALAAGLDKFVFSWVSR 1229
+A +++E DC+ L L+ S + V I LA+ VF V R
Sbjct: 1272 KVAYEAGFRNLVVEMDCKKLFLQLRGKASDVTPFGR--VVDDILYLASKCSNVVFEHVKR 1329
Query: 1230 TGNGFAHGLASWASKQIPGR 1289
N AH LA + R
Sbjct: 1330 HCNKVAHLLAQMCKNAMEKR 1349
>gb|AAD24831.1| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana]
Length = 1524
Score = 86.7 bits (213), Expect = 1e-14
Identities = 89/395 (22%), Positives = 162/395 (41%), Gaps = 15/395 (3%)
Frame = +3
Query: 126 DLKVNSLFSPV--LLSWNQNRLGELFPFXXXXXXXXXXXXPSGSPDHIIWPATKNRELTV 299
++ +N+LF W+ +++ + S PD IIW E TV
Sbjct: 1112 EMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKIIWNYNTTGEYTV 1171
Query: 300 KSAYVFLTKQDNISFSSSFNP---LSMKN-LWKLHLDAAT*LFIWKLYIGGLPTGDVLHK 467
+S Y LT + + + P + +K +W L + F+W+ L T + L
Sbjct: 1172 RSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRALSQALATTERLTT 1231
Query: 468 FKFKGDISCSFCQKCIETASHVFFSCTWIKMMWYVSNAMLDINVHPDWNVKEWINQFIFW 647
+ D C C + E+ +H F+C + M W++S++ L N + +E I+ + +
Sbjct: 1232 RGMRIDPICPRCHRENESINHALFTCPFATMAWWLSDSSLIRNQLMSNDFEENISNILNF 1291
Query: 648 CSSKDEDICRRGYLCLFILYELWLARNKARM-ECRPIELKSILNFSDMKRE--ITSLAFL 818
+ L +++++ +W ARN + R K++L+ + + +
Sbjct: 1292 VQDTTMSDFHK-LLPVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNATQSHK 1350
Query: 819 SITYPGLPLSFNIIXXXXXXXXXVKVNFNVAFDRSHFSAAVGVVVRNAEGV-ILGAAFRR 995
P ++ N I VK NF+ FD A G ++RN G I + +
Sbjct: 1351 KTPSPTRQIAENKIEWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKL 1410
Query: 996 FSANDPEQAELIGAEVAILLALRLNLCFVILEGDCQTLMSALK--TCNSSLLG*NSFFVF 1169
++P +AE A+ V +EGDCQTL++ + + +SSL
Sbjct: 1411 AHTSNPLEAETKALLAALQQTWIRGYTQVFMEGDCQTLINLINGISFHSSLA-------- 1462
Query: 1170 QHIFALAAGLDKFV---FSWVSRTGNGFAHGLASW 1265
H+ ++ +KF F ++ R GN AH LA +
Sbjct: 1463 NHLEDISFWANKFASIQFGFIRRKGNKLAHVLAKY 1497
>gb|EEE66057.1| hypothetical protein OsJ_22054 [Oryza sativa Japonica Group]
Length = 940
Score = 79.0 bits (193), Expect(2) = 2e-14
Identities = 93/421 (22%), Positives = 170/421 (40%), Gaps = 20/421 (4%)
Frame = +3
Query: 78 KPIILKDLLDPGPSLFDLKVNSLFSPVLLSWNQNRLGELFPFXXXXXXXXXXXXPSGSPD 257
KP +++ LL P D+ V+ L + + W+++++ F G D
Sbjct: 517 KPSMVRPLL---PMPDDVTVDFLVNAAIGEWDEDKVFSFFDETTAQQILQIPVSAHGGED 573
Query: 258 HIIWPATKNRELTVKSAYVFLTKQDNISFSSSFNPLSM-----------KNLWKLHLDAA 404
I WP K +V+SAY L + + + S N M K LW+++
Sbjct: 574 FISWPHDKRGVFSVRSAYN-LARSEIFMAAQSENGRGMLSGLQESANRWKELWRINAPGK 632
Query: 405 T*LFIWKLYIGGLPTGDVLHKFKFKGDISCSFCQKCIETASHVFFSCTWIKMMWYVSNAM 584
+W++ LP+G L + C FC++ + H+F C + +W
Sbjct: 633 MLTNLWRIVHDCLPSGFQLRRRHIPATDGCCFCER-DDRIEHIFLLCPFAVCIWDSIKQH 691
Query: 585 LDINVHPD--WNVKEWINQFIFWCSSKDEDICRRGYLCLFILYELWLARNKARME---CR 749
D+ + N+K+W+ F+ S+ + L+ +W ARN +R
Sbjct: 692 FDLKLCMTDLSNMKQWVFDFLGRSSNIQKT------ALAVTLWHIWEARNHSRNNPTLAN 745
Query: 750 PIE-LKSILNFSDMKREITSLAFLSITYPGLPLSFNIIXXXXXXXXXVKVNFNVAFDRSH 926
P + ++ IL + +M + A ++ L + + +N + A +S
Sbjct: 746 PRQVIQKILAYVEMIEQHCCCAVQAVRGDALR---PVPRWRPPPEGTILINTDAAVFQSV 802
Query: 927 FSAAVGVVVRNAEGVILGAAFRRFS-ANDPEQAELIGAEVAILLALRLNLCFVILEGDCQ 1103
S +G + R+ G+ L AA R S PE AE + A+ A+ ++L DC
Sbjct: 803 NSFGLGFLFRDHSGLCLFAANERHSGCIQPEMAEALAIRCALRTAMEEGHQKIVLASDCL 862
Query: 1104 TLMSALKT--CNSSLLG*NSFFVFQHIFALAAGLDKFVFSWVSRTGNGFAHGLASWASKQ 1277
++ +++ + S++G + I LAAG F V+R N AH LA + +
Sbjct: 863 AIIQKIQSGARDRSMVG----ALVSDINFLAAGFLDCSFIHVNRVTNAAAHLLAQCSEQT 918
Query: 1278 I 1280
+
Sbjct: 919 V 919
Score = 27.7 bits (60), Expect(2) = 2e-14
Identities = 10/20 (50%), Positives = 12/20 (60%)
Frame = +2
Query: 2 GFLWNIGAGSSISIFNDPWL 61
G W IG GSS+ I D W+
Sbjct: 494 GVRWGIGNGSSVKILKDHWI 513