BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00015306
         (1126 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI32027.3| unnamed protein product [Vitis vinifera]              364   2e-98
ref|XP_002269566.1| PREDICTED: E3 ubiquitin-protein ligase HERC2...   360   4e-97
ref|XP_003517766.1| PREDICTED: probable E3 ubiquitin-protein lig...   349   6e-94
ref|XP_003519776.1| PREDICTED: probable E3 ubiquitin-protein lig...   347   2e-93
ref|XP_002321756.1| predicted protein [Populus trichocarpa] gi|2...   346   5e-93

>emb|CBI32027.3| unnamed protein product [Vitis vinifera]
          Length = 405

 Score =  364 bits (935), Expect = 2e-98
 Identities = 181/282 (64%), Positives = 220/282 (78%), Gaps = 3/282 (1%)
 Frame = -1

Query: 1027 KVWSWGAGTEGQLGTTKLQDENHPQLIHYLSFTPKH-ISYLACGGAHVIALSREDGKVLS 851
            ++WSWGAGT+GQLGTT+L+DE+ PQL+    F+    IS LACGGAHV+AL+  DGKV++
Sbjct: 21   EIWSWGAGTDGQLGTTRLEDEHLPQLLDLPRFSSAGPISLLACGGAHVLALTT-DGKVVT 79

Query: 850  WGRGTSGQLGHGDMKNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVLTCGDGTFGQ 671
            WGRGTSGQLGHGDM + L P  VK LESFV+++VSAGW+HSGFVS+TG + TCGDG+FGQ
Sbjct: 80   WGRGTSGQLGHGDMVSSLQPKHVKLLESFVMTHVSAGWSHSGFVSDTGCLFTCGDGSFGQ 139

Query: 670  LGHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGN--SGDALYGFGSGKRGQLGT 497
            LGHGD +S C P++V YF S+HV+QI CGMRH+LVLLKG   SGD +YGFGSGKRGQLG 
Sbjct: 140  LGHGDCKSHCSPVKVSYFDSKHVDQIACGMRHSLVLLKGTGLSGDQVYGFGSGKRGQLGV 199

Query: 496  SMDMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGISDACLPLL 317
            SMD  T+S+++PQ   G + V I  I ANGD SAAL+ +GHLYTWG+GF   SD   P  
Sbjct: 200  SMDN-TRSISVPQATVGLEGVEIISIDANGDHSAALSANGHLYTWGRGFGSTSDVHCPRC 258

Query: 316  PSSALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQK 191
              S+L FT+VALGWNH L+LT  G VFMLGGN HG+L  PQK
Sbjct: 259  LPSSLCFTQVALGWNHMLVLTGGGEVFMLGGNRHGVLGDPQK 300


>ref|XP_002269566.1| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Vitis vinifera]
          Length = 400

 Score =  360 bits (924), Expect = 4e-97
 Identities = 179/280 (63%), Positives = 218/280 (77%), Gaps = 1/280 (0%)
 Frame = -1

Query: 1027 KVWSWGAGTEGQLGTTKLQDENHPQLIHYLSFTPKH-ISYLACGGAHVIALSREDGKVLS 851
            ++WSWGAGT+GQLGTT+L+DE+ PQL+    F+    IS LACGGAHV+AL+  DGKV++
Sbjct: 13   EIWSWGAGTDGQLGTTRLEDEHLPQLLDLPRFSSAGPISLLACGGAHVLALTT-DGKVVT 71

Query: 850  WGRGTSGQLGHGDMKNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVLTCGDGTFGQ 671
            WGRGTSGQLGHGDM + L P  VK LESFV+++VSAGW+HSGFVS+TG + TCGDG+FGQ
Sbjct: 72   WGRGTSGQLGHGDMVSSLQPKHVKLLESFVMTHVSAGWSHSGFVSDTGCLFTCGDGSFGQ 131

Query: 670  LGHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGNSGDALYGFGSGKRGQLGTSM 491
            LGHGD +S C P++V YF S+HV+QI CGMRH+LVLLK   GD +YGFGSGKRGQLG SM
Sbjct: 132  LGHGDCKSHCSPVKVSYFDSKHVDQIACGMRHSLVLLK---GDQVYGFGSGKRGQLGVSM 188

Query: 490  DMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGISDACLPLLPS 311
            D  T+S+++PQ   G + V I  I ANGD SAAL+ +GHLYTWG+GF   SD   P    
Sbjct: 189  DN-TRSISVPQATVGLEGVEIISIDANGDHSAALSANGHLYTWGRGFGSTSDVHCPRCLP 247

Query: 310  SALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQK 191
            S+L FT+VALGWNH L+LT  G VFMLGGN HG+L  PQK
Sbjct: 248  SSLCFTQVALGWNHMLVLTGGGEVFMLGGNRHGVLGDPQK 287


>ref|XP_003517766.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC2-like [Glycine
            max]
          Length = 388

 Score =  349 bits (896), Expect = 6e-94
 Identities = 177/281 (62%), Positives = 213/281 (75%), Gaps = 1/281 (0%)
 Frame = -1

Query: 1027 KVWSWGAGTEGQLGTTKLQDENHPQLIHYLSFTPKHISYLACGGAHVIALSREDGKVLSW 848
            +VWSWGAGTEGQLGT  LQDE+ PQL+H  S +   IS LACGGAHVIAL+   GKVLSW
Sbjct: 19   RVWSWGAGTEGQLGTKILQDEHFPQLLHQPSLSS--ISSLACGGAHVIALTSA-GKVLSW 75

Query: 847  GRGTSGQLGHG-DMKNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVLTCGDGTFGQ 671
            GRG SGQLGHG  + N LYP  V  L+ + +++VSAGW HSGFVS+ G V TCGDG+FGQ
Sbjct: 76   GRGNSGQLGHGLVVSNSLYPKAVTSLDGYFITHVSAGWGHSGFVSDNGCVFTCGDGSFGQ 135

Query: 670  LGHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGNSGDALYGFGSGKRGQLGTSM 491
            LGHGD  S C P++V  F  +HVEQ+ CGMRH+LVLLKGN    +YGFGSGKRGQLG S 
Sbjct: 136  LGHGDHASHCSPVKVSCFVDQHVEQVACGMRHSLVLLKGNQ---VYGFGSGKRGQLGVSN 192

Query: 490  DMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGISDACLPLLPS 311
            D + KS+N+P+V+ GF+ V IA I ANGD SAA++VDGH+YTWG+GF G  DA +P   +
Sbjct: 193  DRV-KSVNVPKVVSGFEGVEIAGIAANGDHSAAVSVDGHVYTWGRGFKGFEDARVPQCLN 251

Query: 310  SALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQKL 188
            S+L FTKVALGWNHAL ++  G V MLGGNH G+LS  Q +
Sbjct: 252  SSLNFTKVALGWNHALAMSGEGEVCMLGGNHLGVLSDLQNI 292



 Score = 58.9 bits (141), Expect = 2e-06
 Identities = 53/191 (27%), Positives = 90/191 (47%), Gaps = 14/191 (7%)
 Frame = -1

Query: 1030 NKVWSWGAGTEGQLGTT--KLQDENHPQLIHYLSFTPKHISYLACGGAHVIALSREDGKV 857
            N+V+ +G+G  GQLG +  +++  N P+++    F    I+ +A  G H  A+S  DG V
Sbjct: 175  NQVYGFGSGKRGQLGVSNDRVKSVNVPKVVS--GFEGVEIAGIAANGDHSAAVS-VDGHV 231

Query: 856  LSWGRGTSGQLGHGDMKNCLYPFPVKCLESFV-VSYVSAGWNHSGFVSNTGRVLTCGDGT 680
             +WGRG  G       ++   P   +CL S +  + V+ GWNH+  +S  G V   G   
Sbjct: 232  YTWGRGFKG------FEDARVP---QCLNSSLNFTKVALGWNHALAMSGEGEVCMLGGNH 282

Query: 679  FGQLGHGDFQSQCLPIEVMYFRSRHVEQ-----------ITCGMRHTLVLLKGNSGDALY 533
             G L   D Q+      + + R  ++E+           I  G  H++++ +      + 
Sbjct: 283  LGVL--SDLQNISPAKHLPHLREVNLEKVPGLDGTKITDIATGAEHSVIVTEHGE---IK 337

Query: 532  GFGSGKRGQLG 500
             +G G+ GQLG
Sbjct: 338  TWGWGEHGQLG 348


>ref|XP_003519776.1| PREDICTED: probable E3 ubiquitin-protein ligase HERC1-like [Glycine
            max]
          Length = 388

 Score =  347 bits (891), Expect = 2e-93
 Identities = 174/281 (61%), Positives = 212/281 (75%), Gaps = 1/281 (0%)
 Frame = -1

Query: 1027 KVWSWGAGTEGQLGTTKLQDENHPQLIHYLSFTPKHISYLACGGAHVIALSREDGKVLSW 848
            +VWSWGAGTEGQLGT  LQDE+ PQL+H  S +   IS LACGGAHVIAL+   GK LSW
Sbjct: 19   RVWSWGAGTEGQLGTKILQDEHFPQLLHQFSLSS--ISSLACGGAHVIALTSA-GKALSW 75

Query: 847  GRGTSGQLGHGDM-KNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVLTCGDGTFGQ 671
            GRG SGQLGHG++  N LYP  V  L+ + +++VSAGW+HSGFVS+TG V TCGDG+FGQ
Sbjct: 76   GRGNSGQLGHGEVVSNTLYPKAVTSLDGYFITHVSAGWSHSGFVSDTGFVFTCGDGSFGQ 135

Query: 670  LGHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGNSGDALYGFGSGKRGQLGTSM 491
            LGHGD  S C P++V  F   HV Q+ CGMRH+LVLLKGN    +YGFGSGKRGQLG S 
Sbjct: 136  LGHGDNASHCSPVKVSCFVDLHVAQVACGMRHSLVLLKGNQ---VYGFGSGKRGQLGVSN 192

Query: 490  DMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGISDACLPLLPS 311
            D + KS+N+P+V+ GF+ V I    ANGD SAA++VDGH+YTWG+GF G  DA +P   +
Sbjct: 193  DRV-KSVNVPKVVSGFEGVEIVGTAANGDHSAAVSVDGHVYTWGRGFKGFEDAHVPQCLN 251

Query: 310  SALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQKL 188
            S+L FTKVALGWNHAL ++  G V+MLGGNH G+LS  Q +
Sbjct: 252  SSLNFTKVALGWNHALAMSGEGEVYMLGGNHLGVLSDLQNI 292


>ref|XP_002321756.1| predicted protein [Populus trichocarpa] gi|222868752|gb|EEF05883.1|
            predicted protein [Populus trichocarpa]
          Length = 390

 Score =  346 bits (888), Expect = 5e-93
 Identities = 175/286 (61%), Positives = 215/286 (75%), Gaps = 2/286 (0%)
 Frame = -1

Query: 1039 SINNKVWSWGAGTEGQLGTTKLQDENHPQLIH--YLSFTPKHISYLACGGAHVIALSRED 866
            S   ++WSWGAGTEGQLGT KL+DE  PQL+H  +LS + + IS LACGGAHVIAL+   
Sbjct: 11   SKQQQIWSWGAGTEGQLGTGKLEDEYLPQLLHLPFLS-SAESISTLACGGAHVIALT-SG 68

Query: 865  GKVLSWGRGTSGQLGHGDMKNCLYPFPVKCLESFVVSYVSAGWNHSGFVSNTGRVLTCGD 686
            G+V +WGRGT+GQLGHG+M N L+P PV  L+S V+++VSAGW+HSGFVS+ G + TCGD
Sbjct: 69   GRVFTWGRGTTGQLGHGEMLNSLHPKPVSSLQSCVITHVSAGWSHSGFVSDIGLLFTCGD 128

Query: 685  GTFGQLGHGDFQSQCLPIEVMYFRSRHVEQITCGMRHTLVLLKGNSGDALYGFGSGKRGQ 506
            G+FGQLG G+++SQ  P++V YF +  V QI CGMRH+LVLL GN    +YGFGSGKRGQ
Sbjct: 129  GSFGQLGQGNYRSQSTPVKVDYFANNFVNQIACGMRHSLVLLNGNQ---VYGFGSGKRGQ 185

Query: 505  LGTSMDMITKSLNLPQVIFGFQQVNIARIIANGDQSAALTVDGHLYTWGKGFHGISDACL 326
            LG S D  TKS+NLPQV  G + V I  I ANGD SAA++ DGHLYTWG+GF G SDA  
Sbjct: 186  LGISRDK-TKSINLPQVTCGLEDVQIVSISANGDHSAAISADGHLYTWGRGFAGASDANF 244

Query: 325  PLLPSSALKFTKVALGWNHALLLTDCGTVFMLGGNHHGMLSSPQKL 188
            P L  S+L+ TK ALGWNH LLLT    V MLGGN+HG+L   +K+
Sbjct: 245  PQLSLSSLRCTKAALGWNHGLLLTGDEEVLMLGGNYHGVLCDLEKM 290