BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00014020
         (1484 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEW67998.1| gibberellin 3 oxidase 1 [Castanea mollissima]          584   e-164
dbj|BAD91162.1| gibberellin 3-beta hydroxylase [Prunus subhirtella]   577   e-162
ref|XP_002303276.1| gibberellin 3-oxidase [Populus trichocarpa] ...   572   e-161
gb|AAR12160.1| gibberellin 3-oxidase [Populus tremula x Populus ...   572   e-161
ref|XP_002284963.2| PREDICTED: gibberellin 3-beta-dioxygenase 1-...   566   e-159

>gb|AEW67998.1| gibberellin 3 oxidase 1 [Castanea mollissima]
          Length = 368

 Score =  584 bits (1505), Expect = e-164
 Identities = 274/368 (74%), Positives = 320/368 (86%)
 Frame = -3

Query: 1320 MPSRLSDAFRAHPVHLHHNHLDFETVQEVPDSHAWPALVDYPSENLHDAESVPIIDLMDP 1141
            MPSRLSDAF+AHPV L+  H DF ++QE+PDS+ W  L +YPS     +ESVP+IDL DP
Sbjct: 1    MPSRLSDAFKAHPVQLYPKHQDFNSLQELPDSYKWTQLDEYPSCESFSSESVPLIDLTDP 60

Query: 1140 NAAKLVGNACETWGVFQVTNHGIPMRLLEDLESEGRRLFFLPAKQKLKAARSPDGISGYG 961
            NA  LVG+AC+TWGVFQVTNHGIP +LL+D+ES  R LF LP +QKLKAARSPDG+SGYG
Sbjct: 61   NALTLVGHACKTWGVFQVTNHGIPTKLLDDIESVSRCLFSLPQQQKLKAARSPDGVSGYG 120

Query: 960  IARISSFFPKLMWSEGFTIVGSPVEHARQLWPQDYIKFCDVMEEYEKAMKKLANRLMWIM 781
            +ARISSFFPKLMWSEGFTIVGSP+EH RQLWPQ+Y KFCD++EEYEK MKKLA RLMW++
Sbjct: 121  LARISSFFPKLMWSEGFTIVGSPLEHFRQLWPQEYSKFCDIIEEYEKEMKKLAGRLMWLI 180

Query: 780  LGSLGITGEDLKWASPKGDIKSACAALQLNSYPACPDPNRAMGLAAHTDSTLLTVLYQNS 601
            LGSLGI  ED+KWA PKG+ + A AALQLNSYPACPDP++AMGLAAHTDSTLLT+L+QN+
Sbjct: 181  LGSLGICNEDIKWAGPKGEFQGASAALQLNSYPACPDPDQAMGLAAHTDSTLLTILHQNN 240

Query: 600  TSGLQVLREGAGWVTVPPTPGALVVNVGDLLHILSNGRYVSVLHRAVVHRTRHRLSIAYL 421
            TSGLQVLREG GWVTV P  GALVVN+GDLLHILSNG Y SVLHRA+V+RTR+RLS+AYL
Sbjct: 241  TSGLQVLREGTGWVTVDPVQGALVVNIGDLLHILSNGLYPSVLHRAMVNRTRYRLSMAYL 300

Query: 420  YGPPTNIQISPLSKLIDPKHPPLYRSVTWSEYLGTKAKHFNKALSSVRLCGPKNGFIHLN 241
            YGPP ++QISPLSKL+ P HPPLYR VTW+EYLGTKAKHFNKALS VRLC P NG + +N
Sbjct: 301  YGPPDSVQISPLSKLLGPSHPPLYRPVTWNEYLGTKAKHFNKALSCVRLCVPLNGLVDVN 360

Query: 240  DHSRMKLG 217
            DH+R+++G
Sbjct: 361  DHNRVQVG 368


>dbj|BAD91162.1| gibberellin 3-beta hydroxylase [Prunus subhirtella]
          Length = 372

 Score =  577 bits (1487), Expect = e-162
 Identities = 275/372 (73%), Positives = 319/372 (85%), Gaps = 4/372 (1%)
 Frame = -3

Query: 1320 MPSRLSDAFRAHPVHLHHNHLDFETVQEVPDSHAWPALVD-YPSENLH---DAESVPIID 1153
            MP+RLSDAF++HP++L   H DF ++QE+PDS+AW    D YPS       + +SVP+ID
Sbjct: 1    MPARLSDAFKSHPLNLQLKHPDFSSLQELPDSYAWSQNDDQYPSSPGSFGAEQDSVPVID 60

Query: 1152 LMDPNAAKLVGNACETWGVFQVTNHGIPMRLLEDLESEGRRLFFLPAKQKLKAARSPDGI 973
            L DPNA KL G+AC TWGVFQVTNHGIP +LL+D+ES GR LF LP +QKLKAARSPDGI
Sbjct: 61   LSDPNALKLTGHACRTWGVFQVTNHGIPSKLLDDIESAGRSLFSLPVQQKLKAARSPDGI 120

Query: 972  SGYGIARISSFFPKLMWSEGFTIVGSPVEHARQLWPQDYIKFCDVMEEYEKAMKKLANRL 793
            SGYG ARISSFF KLMWSEGFTIVGSP++H RQLWPQDY KFC+++EEYEK MK+LA RL
Sbjct: 121  SGYGFARISSFFQKLMWSEGFTIVGSPLDHFRQLWPQDYNKFCNIIEEYEKVMKRLAGRL 180

Query: 792  MWIMLGSLGITGEDLKWASPKGDIKSACAALQLNSYPACPDPNRAMGLAAHTDSTLLTVL 613
            MW+MLGSLGI+ ED+KWA PKGD + A AALQLNSYPACPDP+RAMGLAAHTDSTLLT+L
Sbjct: 181  MWLMLGSLGISMEDVKWAGPKGDFRDASAALQLNSYPACPDPDRAMGLAAHTDSTLLTIL 240

Query: 612  YQNSTSGLQVLREGAGWVTVPPTPGALVVNVGDLLHILSNGRYVSVLHRAVVHRTRHRLS 433
            YQN+TSGLQVLREG GWVTVPP PGALVVNVGDL+HILSNG Y SVLHRAVV+R+RHRLS
Sbjct: 241  YQNNTSGLQVLREGTGWVTVPPLPGALVVNVGDLIHILSNGLYPSVLHRAVVNRSRHRLS 300

Query: 432  IAYLYGPPTNIQISPLSKLIDPKHPPLYRSVTWSEYLGTKAKHFNKALSSVRLCGPKNGF 253
            IAYLYGPP ++QISPLSKL+ P  PPLYR +TW+EYLGTKAKHFNKALSSVRLC P N  
Sbjct: 301  IAYLYGPPASVQISPLSKLLGPSQPPLYRPITWNEYLGTKAKHFNKALSSVRLCAPLNAL 360

Query: 252  IHLNDHSRMKLG 217
            + ++DH+ +K+G
Sbjct: 361  VDVSDHNSVKVG 372


>ref|XP_002303276.1| gibberellin 3-oxidase [Populus trichocarpa]
            gi|222840708|gb|EEE78255.1| gibberellin 3-oxidase
            [Populus trichocarpa]
          Length = 373

 Score =  572 bits (1475), Expect = e-161
 Identities = 271/373 (72%), Positives = 313/373 (83%), Gaps = 5/373 (1%)
 Frame = -3

Query: 1320 MPSR-LSDAFRAHPVHLHHNHLDFETVQEVPDSHAWPALVD----YPSENLHDAESVPII 1156
            MPSR L+DAFR+HPVHLH  HLDF ++QE+PDSH W  L D    +PS      ESVP+I
Sbjct: 1    MPSRSLTDAFRSHPVHLHQKHLDFSSLQEIPDSHKWTQLDDIEQQHPSVESFITESVPVI 60

Query: 1155 DLMDPNAAKLVGNACETWGVFQVTNHGIPMRLLEDLESEGRRLFFLPAKQKLKAARSPDG 976
            DL+DPN  + +GNAC+TWGV QVTNHGIP+ LLE +E   R LF LP +QKLKAARSPDG
Sbjct: 61   DLLDPNVLQNIGNACKTWGVLQVTNHGIPISLLESVEGVSRSLFSLPVQQKLKAARSPDG 120

Query: 975  ISGYGIARISSFFPKLMWSEGFTIVGSPVEHARQLWPQDYIKFCDVMEEYEKAMKKLANR 796
            +SGYG+ARISSFF KLMWSEGFTIVGSP+EH RQLWPQDY KFCDV+EE+EK M+KLA R
Sbjct: 121  VSGYGVARISSFFSKLMWSEGFTIVGSPLEHFRQLWPQDYTKFCDVIEEHEKEMQKLARR 180

Query: 795  LMWIMLGSLGITGEDLKWASPKGDIKSACAALQLNSYPACPDPNRAMGLAAHTDSTLLTV 616
            L W+MLGSLGIT +DL WA PKG+ K   AALQLNSYPACPDP+ AMGLAAHTDSTLLT+
Sbjct: 181  LTWLMLGSLGITKKDLNWAGPKGESKEGGAALQLNSYPACPDPDLAMGLAAHTDSTLLTI 240

Query: 615  LYQNSTSGLQVLREGAGWVTVPPTPGALVVNVGDLLHILSNGRYVSVLHRAVVHRTRHRL 436
            LYQN+TSGLQVL+EG GWVTVPP PG LVVNVGDLLHILSNG Y SVLHRAVV+RT+HRL
Sbjct: 241  LYQNNTSGLQVLKEGIGWVTVPPIPGGLVVNVGDLLHILSNGLYPSVLHRAVVNRTKHRL 300

Query: 435  SIAYLYGPPTNIQISPLSKLIDPKHPPLYRSVTWSEYLGTKAKHFNKALSSVRLCGPKNG 256
            SIAYLYGPP+++QISP+ KL+ P HPPLYR +TW+EYL  KAKHFNKALSSVR+C P NG
Sbjct: 301  SIAYLYGPPSSVQISPIQKLVGPNHPPLYRPITWNEYLVAKAKHFNKALSSVRICAPLNG 360

Query: 255  FIHLNDHSRMKLG 217
             + +NDH+ +K+G
Sbjct: 361  LVDVNDHNSVKVG 373


>gb|AAR12160.1| gibberellin 3-oxidase [Populus tremula x Populus tremuloides]
          Length = 373

 Score =  572 bits (1475), Expect = e-161
 Identities = 271/373 (72%), Positives = 313/373 (83%), Gaps = 5/373 (1%)
 Frame = -3

Query: 1320 MPSR-LSDAFRAHPVHLHHNHLDFETVQEVPDSHAWPALVD----YPSENLHDAESVPII 1156
            MPSR L+DAFR+HPVHLH  HLDF ++QE+PDSH W  L D    +PS      ESVP+I
Sbjct: 1    MPSRSLADAFRSHPVHLHQKHLDFSSLQEIPDSHKWTQLDDIEQQHPSVESFITESVPVI 60

Query: 1155 DLMDPNAAKLVGNACETWGVFQVTNHGIPMRLLEDLESEGRRLFFLPAKQKLKAARSPDG 976
            DL+DPN  + +GNAC+TWGV QVTNHGIP+ LLE +E   R LF LP +QKLKAARSPDG
Sbjct: 61   DLLDPNVLQNIGNACKTWGVLQVTNHGIPISLLESVEGVSRSLFSLPVQQKLKAARSPDG 120

Query: 975  ISGYGIARISSFFPKLMWSEGFTIVGSPVEHARQLWPQDYIKFCDVMEEYEKAMKKLANR 796
            +SGYG+ARISSFF KLMWSEGFTIVGSP+EH RQLWPQDY KFCDV+EE+EK M+KLA R
Sbjct: 121  VSGYGVARISSFFSKLMWSEGFTIVGSPLEHFRQLWPQDYTKFCDVIEEHEKEMQKLARR 180

Query: 795  LMWIMLGSLGITGEDLKWASPKGDIKSACAALQLNSYPACPDPNRAMGLAAHTDSTLLTV 616
            L W+MLGSLGIT +DL WA PKG+ K   AALQLNSYPACPDP+ AMGLAAHTDSTLLT+
Sbjct: 181  LTWLMLGSLGITKKDLNWAGPKGESKEGGAALQLNSYPACPDPDLAMGLAAHTDSTLLTI 240

Query: 615  LYQNSTSGLQVLREGAGWVTVPPTPGALVVNVGDLLHILSNGRYVSVLHRAVVHRTRHRL 436
            LYQN+TSGLQVL+EG GWVTVPP PG LVVNVGDLLHILSNG Y SVLHRAVV+RT+HRL
Sbjct: 241  LYQNNTSGLQVLKEGIGWVTVPPIPGGLVVNVGDLLHILSNGLYPSVLHRAVVNRTKHRL 300

Query: 435  SIAYLYGPPTNIQISPLSKLIDPKHPPLYRSVTWSEYLGTKAKHFNKALSSVRLCGPKNG 256
            SIAYLYGPP+++QISP+ KL+ P HPPLYR +TW+EYL  KAKHFNKALSSVR+C P NG
Sbjct: 301  SIAYLYGPPSSVQISPIQKLVGPNHPPLYRPITWNEYLVAKAKHFNKALSSVRICAPLNG 360

Query: 255  FIHLNDHSRMKLG 217
             + +NDH+ +K+G
Sbjct: 361  LVDVNDHNSVKVG 373


>ref|XP_002284963.2| PREDICTED: gibberellin 3-beta-dioxygenase 1-like [Vitis vinifera]
            gi|297743334|emb|CBI36201.3| unnamed protein product
            [Vitis vinifera]
          Length = 365

 Score =  566 bits (1459), Expect = e-159
 Identities = 267/368 (72%), Positives = 315/368 (85%)
 Frame = -3

Query: 1320 MPSRLSDAFRAHPVHLHHNHLDFETVQEVPDSHAWPALVDYPSENLHDAESVPIIDLMDP 1141
            MPSR+SDAF+AHP+HL+H HLD  +VQE+PD +AW  + + PS +    ESVP+IDL DP
Sbjct: 1    MPSRISDAFKAHPLHLNHRHLDLNSVQELPDLYAWAGVDENPSGDSLITESVPVIDLTDP 60

Query: 1140 NAAKLVGNACETWGVFQVTNHGIPMRLLEDLESEGRRLFFLPAKQKLKAARSPDGISGYG 961
            NA++LVG+AC++WGVFQVTNHGIP  LL+D+ES GR LF LPA+QKLKAARSPDG++GYG
Sbjct: 61   NASELVGHACKSWGVFQVTNHGIPGSLLDDIESAGRSLFSLPAQQKLKAARSPDGVAGYG 120

Query: 960  IARISSFFPKLMWSEGFTIVGSPVEHARQLWPQDYIKFCDVMEEYEKAMKKLANRLMWIM 781
            +ARISSFF KLMW EGFTI GSP+EHARQLWPQDY KFCDV EE+EK M +LA RLMW+M
Sbjct: 121  LARISSFFNKLMWYEGFTIFGSPLEHARQLWPQDYTKFCDVTEEFEKEMNQLAERLMWLM 180

Query: 780  LGSLGITGEDLKWASPKGDIKSACAALQLNSYPACPDPNRAMGLAAHTDSTLLTVLYQNS 601
            LGSLGIT EDL WA  KGD K   AALQLNSYPACP+P+RAMGLAAHTDS+L T+LYQN+
Sbjct: 181  LGSLGITKEDLNWAGSKGDFK---AALQLNSYPACPEPDRAMGLAAHTDSSLFTILYQNT 237

Query: 600  TSGLQVLREGAGWVTVPPTPGALVVNVGDLLHILSNGRYVSVLHRAVVHRTRHRLSIAYL 421
             SGLQV REGAGW+TVPP PGALV+NVGDLLHILSNG + SV+HRA+V+RT+HRLS+AYL
Sbjct: 238  VSGLQVQREGAGWITVPPLPGALVINVGDLLHILSNGVFPSVVHRALVNRTKHRLSVAYL 297

Query: 420  YGPPTNIQISPLSKLIDPKHPPLYRSVTWSEYLGTKAKHFNKALSSVRLCGPKNGFIHLN 241
            YGPP  + ISP+ KL+D  HPPLYR VTWSEYL TKAKHF+KALS VRLC P+NGFI +N
Sbjct: 298  YGPPAGVPISPVPKLVDSTHPPLYRPVTWSEYLCTKAKHFDKALSLVRLCMPRNGFIDVN 357

Query: 240  DHSRMKLG 217
            DH+ +K+G
Sbjct: 358  DHNGVKVG 365