BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00013612
         (1719 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]             554   e-155
emb|CBI24150.3| unnamed protein product [Vitis vinifera]              525   e-146
ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chlorop...   516   e-144
ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precurso...   506   e-141
gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]                  498   e-138

>gb|AEF32083.1| ent-kaurene synthase [Castanea mollissima]
          Length = 784

 Score =  554 bits (1428), Expect = e-155
 Identities = 284/486 (58%), Positives = 352/486 (72%), Gaps = 4/486 (0%)
 Frame = -2

Query: 1718 VLDETYRCWLRREEEIFLDIETCAMAFRLLRTNGFVVSSDTLAEFNEEKDFFTSAGGNIK 1539
            VLDETYRCWL+ EEEIFLD  TCAMAFR+LR NGF +SSD   + +E+  F +S GG +K
Sbjct: 298  VLDETYRCWLQGEEEIFLDTATCAMAFRILRVNGFDISSDPFTQLSEDH-FSSSLGGYMK 356

Query: 1538 GIHTVLELYKTSQIMILPNEPVLEILNSWTSQLLKQNLANGVIHDGNCQK----EVVNSL 1371
             I +VLEL++ SQI+I P+E VLE  N WTSQ L Q L+NG IH     K    EV ++L
Sbjct: 357  DIGSVLELFRASQIIIHPDEFVLEKQNFWTSQFLIQELSNGSIHADGLNKYVSQEVDDAL 416

Query: 1370 KFTYDADVERLENRRNIECYNVDSLWVLKASYRCSNIDNRDIVQFGVEDYNFCQGILQKE 1191
            KF Y A +ERL NRR IE YN ++  VLK +Y  SNI N D +   VED+N CQ I ++E
Sbjct: 417  KFPYHASLERLSNRRAIENYNKNNTRVLKTAYSSSNIGNEDFLNLAVEDFNICQSIQREE 476

Query: 1190 LANLERWVKENRLDQLKFARLKLEYLYFSVAGILFSPELSDARMSWTKNSFLSTVVDDFF 1011
            L +L RW+ ENRLD+LKFAR KL Y YFS A  LFSPELSDAR+SW KN  L+TVVDDFF
Sbjct: 477  LKDLARWITENRLDKLKFARQKLAYCYFSAAATLFSPELSDARISWAKNGVLTTVVDDFF 536

Query: 1010 DVKGSREELVNLINLVEKWERDTATDYSSEQVEIIFLAIHKTINDLGDIAFTRQGRNIER 831
            DV GS EELVNLI LVEKW+ D +TD  SE VEIIF A+H TI D  D   T QGRN+  
Sbjct: 537  DVGGSVEELVNLIQLVEKWDVDVSTDCCSENVEIIFSALHSTICDFADKGLTLQGRNVIS 596

Query: 830  HMIDLWLSLLNSMMKEYEWVRDKSAPKVEEYIETASISFTLGPVVLVTQYFLGPKLSEEV 651
            H+ID+WL+LL SM+KE EW+RDKS P ++EY+    +SF LGP+VL   Y +GPKLSEE+
Sbjct: 597  HIIDIWLNLLKSMLKEAEWLRDKSVPSMDEYMTNGYVSFALGPIVLPALYCVGPKLSEEI 656

Query: 650  ITSPEYNDLFKHTNICCRLLNDVQTFKREGEQGKLNSVLLCKIHSPEAITEQQAVREIQS 471
            + +PE + L++  + C RLLND+QTFKRE E+GKLN+V LC IH     T+++ ++E++S
Sbjct: 657  VGTPELHHLYEIMSTCGRLLNDIQTFKRESEEGKLNAVSLCMIHGGGDCTKEETIKELKS 716

Query: 470  IIDRNRRELLGLVLDTKGSIVPKACKDLFWNTFRVTHLFYRKKDGFSSPLEMVNAVNAVI 291
             I   RRELL LVL  KGS+VP+ACKDLFW   +V HLFY K DGF+S  EM N+VNAV+
Sbjct: 717  FIAGKRRELLKLVLQEKGSVVPRACKDLFWKMIKVLHLFYMKDDGFTSH-EMFNSVNAVL 775

Query: 290  DEPISL 273
            +EPI L
Sbjct: 776  EEPIVL 781


>emb|CBI24150.3| unnamed protein product [Vitis vinifera]
          Length = 826

 Score =  525 bits (1351), Expect = e-146
 Identities = 263/486 (54%), Positives = 347/486 (71%), Gaps = 4/486 (0%)
 Frame = -2

Query: 1718 VLDETYRCWLRREEEIFLDIETCAMAFRLLRTNGFVVSSDTLAEFNEEKDFFTSAGGNIK 1539
            VLDETYRCWL+REEEIF D  TCA+AFR+LR NG+ +SS  LA+F E+  +F   G + K
Sbjct: 340  VLDETYRCWLQREEEIFADRATCAIAFRILRLNGYDISSVPLAQFAEDDQYF-KFGQDFK 398

Query: 1538 GIHTVLELYKTSQIMILPNEPVLEILNSWTSQLLKQNLANGVIHDGNCQK----EVVNSL 1371
             +   LEL++ S+++I P+E VLE  NSW+S  L+Q L+N  IH     K    EV ++L
Sbjct: 399  DLGAALELFRASEMIIHPDEVVLEKQNSWSSHFLRQGLSNSSIHADRLNKYIAQEVEDAL 458

Query: 1370 KFTYDADVERLENRRNIECYNVDSLWVLKASYRCSNIDNRDIVQFGVEDYNFCQGILQKE 1191
            +F Y A+++R+ NRR+IE YNVD   +LK +YR S++ N+D ++  VED+NFCQ I Q E
Sbjct: 459  RFPYYANLDRIANRRSIEHYNVDDTRILKTAYRSSHVCNKDFLKLAVEDFNFCQSIHQNE 518

Query: 1190 LANLERWVKENRLDQLKFARLKLEYLYFSVAGILFSPELSDARMSWTKNSFLSTVVDDFF 1011
            L  LERW+ ENRLD+LKFAR KL Y YFS A  +FSPE SDAR+SW KNS L+TVVDDFF
Sbjct: 519  LKQLERWIIENRLDKLKFARQKLAYCYFSAAATIFSPEQSDARLSWAKNSVLTTVVDDFF 578

Query: 1010 DVKGSREELVNLINLVEKWERDTATDYSSEQVEIIFLAIHKTINDLGDIAFTRQGRNIER 831
            D+ GS EEL+NLI LVEKW+ D A D  SEQVEI+F A+H TI+++G  A   Q RN+  
Sbjct: 579  DIGGSEEELLNLIQLVEKWDIDVAVDCCSEQVEIVFSALHSTISEIGVKASAWQARNVTS 638

Query: 830  HMIDLWLSLLNSMMKEYEWVRDKSAPKVEEYIETASISFTLGPVVLVTQYFLGPKLSEEV 651
            H+ID+WL LL SM++E +WV +KSAP ++EY+  A +SF LGP+VL   YF+GPKLSEEV
Sbjct: 639  HIIDIWLKLLRSMLQEAQWVSNKSAPTMDEYMTNAYVSFALGPIVLPALYFVGPKLSEEV 698

Query: 650  ITSPEYNDLFKHTNICCRLLNDVQTFKREGEQGKLNSVLLCKIHSPEAITEQQAVREIQS 471
            +  PE + L+K  + C RLLND+ +FKRE ++GK N++ L  IH     TE+QA+RE++ 
Sbjct: 699  VEGPECHKLYKLMSTCGRLLNDIHSFKRESKEGKANALALHMIHGNGVTTEEQAIREMKG 758

Query: 470  IIDRNRRELLGLVLDTKGSIVPKACKDLFWNTFRVTHLFYRKKDGFSSPLEMVNAVNAVI 291
            ++   RREL  LVL  KGS VP+ CKDLFW   +V H FY K DGF+S  +M+ AV +VI
Sbjct: 759  LVKSQRRELQRLVLQEKGSTVPRICKDLFWKMSKVLHTFYEKDDGFTSH-DMLRAVKSVI 817

Query: 290  DEPISL 273
             EP+ L
Sbjct: 818  YEPVLL 823


>ref|XP_002265005.2| PREDICTED: ent-kaur-16-ene synthase, chloroplastic-like [Vitis
            vinifera]
          Length = 793

 Score =  516 bits (1328), Expect = e-144
 Identities = 260/486 (53%), Positives = 342/486 (70%), Gaps = 4/486 (0%)
 Frame = -2

Query: 1718 VLDETYRCWLRREEEIFLDIETCAMAFRLLRTNGFVVSSDTLAEFNEEKDFFTSAGGNIK 1539
            VLDETYRCWL+REEEIF D  TCA+AFR+LR NG+ +SSD         D +   G + K
Sbjct: 315  VLDETYRCWLQREEEIFADRATCAIAFRILRLNGYDISSD---------DQYFKFGQDFK 365

Query: 1538 GIHTVLELYKTSQIMILPNEPVLEILNSWTSQLLKQNLANGVIHDGNCQK----EVVNSL 1371
             +   LEL++ S+++I P+E VLE  NSW+S  L+Q L+N  IH     K    EV ++L
Sbjct: 366  DLGAALELFRASEMIIHPDEVVLEKQNSWSSHFLRQGLSNSSIHADRLNKYIAQEVEDAL 425

Query: 1370 KFTYDADVERLENRRNIECYNVDSLWVLKASYRCSNIDNRDIVQFGVEDYNFCQGILQKE 1191
            +F Y A+++R+ NRR+IE YNVD   +LK +YR S++ N+D ++  VED+NFCQ I Q E
Sbjct: 426  RFPYYANLDRIANRRSIEHYNVDDTRILKTAYRSSHVCNKDFLKLAVEDFNFCQSIHQNE 485

Query: 1190 LANLERWVKENRLDQLKFARLKLEYLYFSVAGILFSPELSDARMSWTKNSFLSTVVDDFF 1011
            L  LERW+ ENRLD+LKFAR KL Y YFS A  +FSPE SDAR+SW KNS L+TVVDDFF
Sbjct: 486  LKQLERWIIENRLDKLKFARQKLAYCYFSAAATIFSPEQSDARLSWAKNSVLTTVVDDFF 545

Query: 1010 DVKGSREELVNLINLVEKWERDTATDYSSEQVEIIFLAIHKTINDLGDIAFTRQGRNIER 831
            D+ GS EEL+NLI LVEKW+ D A D  SEQVEI+F A+H TI+++G  A   Q RN+  
Sbjct: 546  DIGGSEEELLNLIQLVEKWDIDVAVDCCSEQVEIVFSALHSTISEIGVKASAWQARNVTS 605

Query: 830  HMIDLWLSLLNSMMKEYEWVRDKSAPKVEEYIETASISFTLGPVVLVTQYFLGPKLSEEV 651
            H+ID+WL LL SM++E +WV +KSAP ++EY+  A +SF LGP+VL   YF+GPKLSEEV
Sbjct: 606  HIIDIWLKLLRSMLQEAQWVSNKSAPTMDEYMTNAYVSFALGPIVLPALYFVGPKLSEEV 665

Query: 650  ITSPEYNDLFKHTNICCRLLNDVQTFKREGEQGKLNSVLLCKIHSPEAITEQQAVREIQS 471
            +  PE + L+K  + C RLLND+ +FKRE ++GK N++ L  IH     TE+QA+RE++ 
Sbjct: 666  VEGPECHKLYKLMSTCGRLLNDIHSFKRESKEGKANALALHMIHGNGVTTEEQAIREMKG 725

Query: 470  IIDRNRRELLGLVLDTKGSIVPKACKDLFWNTFRVTHLFYRKKDGFSSPLEMVNAVNAVI 291
            ++   RREL  LVL  KGS VP+ CKDLFW   +V H FY K DGF+S  +M+ AV +VI
Sbjct: 726  LVKSQRRELQRLVLQEKGSTVPRICKDLFWKMSKVLHTFYEKDDGFTSH-DMLRAVKSVI 784

Query: 290  DEPISL 273
             EP+ L
Sbjct: 785  YEPVLL 790


>ref|XP_002533694.1| Ent-kaurene synthase B, chloroplast precursor, putative [Ricinus
            communis] gi|223526405|gb|EEF28689.1| Ent-kaurene
            synthase B, chloroplast precursor, putative [Ricinus
            communis]
          Length = 808

 Score =  506 bits (1303), Expect = e-141
 Identities = 272/525 (51%), Positives = 351/525 (66%), Gaps = 12/525 (2%)
 Frame = -2

Query: 1718 VLDETYRCWLRREEEIFLDIETCAMAFRLLRTNGFVVSSDTLAEFNEEKDFFTSAGGNIK 1539
            VL+ETYR WL+  EEIFLD  TCAMAFR+LR NG+ VSSD   +F E+  FF S GG +K
Sbjct: 253  VLEETYRYWLQGNEEIFLDCITCAMAFRILRVNGYDVSSDVFTQFTEDH-FFDSLGGYLK 311

Query: 1538 GIHTVLELYKTSQIMILPNEPVLEILNSWTSQLLKQNLANGVIH-DG--NCQKEVV-NSL 1371
               TVLELY+ SQI+  P+EP+LE  NSWT+  L++ L++G  + DG   C  EVV N+L
Sbjct: 312  DTRTVLELYRASQILY-PDEPLLEKQNSWTNHFLEKCLSSGSSYADGPRECITEVVHNAL 370

Query: 1370 KFTYDADVERLENRRNIECYNVDSLWVLKASYRCSNIDNRDIVQFGVEDYNFCQGILQKE 1191
               Y AD+ERL NRR+IE YNVD   +LKASYRC N  N+  ++  VED+N CQ I Q+E
Sbjct: 371  NCPYYADLERLTNRRSIENYNVDETRILKASYRCLNTGNQHFLKLAVEDFNLCQLIHQEE 430

Query: 1190 LANLERWVKENRLDQLKFARLKLEYLYFSVAGILFSPELSDARMSWTKNSFLSTVVDDFF 1011
            L  L RWV E RL++LKFAR KL Y YFS A  LF+PELSDAR+SW KN  L+TVVDDFF
Sbjct: 431  LQQLGRWVVEKRLNKLKFARQKLGYCYFSAAATLFAPELSDARLSWAKNGVLTTVVDDFF 490

Query: 1010 DVKGSREELVNLINLVEKWERDTATDYSSEQVEIIFLAIHKTINDLGDIAFTRQGRNIER 831
            DV GS EEL+NLI L+EKW+ D +T + SEQVEIIF A+  TI+++GD AFT QGR +  
Sbjct: 491  DVGGSVEELINLIQLIEKWDVDESTHFCSEQVEIIFSALRSTISEIGDKAFTWQGRKVTS 550

Query: 830  HMIDLWLSLLNSMMKEYEWVRDKSAPKVEEYIETASISFTLGPVVLVTQYFLGPKLSEEV 651
            H+I +WL LL SM+ E  W + KS P ++EY+    +SF LGP+VL   + +GPKL+EE 
Sbjct: 551  HVIKIWLDLLKSMLTETLWTKSKSIPTLDEYMINGYVSFALGPIVLPALFLVGPKLTEED 610

Query: 650  ITSPEYNDLFKHTNICCRLLNDVQTFKREGEQGKLNSVLLCKIHSPEAITEQQAVREIQS 471
            +  PE +DLFK    C RLLND + F+RE ++GKLN+V L  I     + E++A+R+I+ 
Sbjct: 611  VRDPELHDLFKAMGTCGRLLNDWRGFQRESKEGKLNAVSLHMIQGNGGVNEEEAIRKIKG 670

Query: 470  IIDRNRRELLGLVLDTKGSIVPKACKDLFWNTFRVTHLFYRKKDGFSSPLEMVNAVNAVI 291
            +I+  R ELL LVL  K S +P+ACKDLFW   +V HLFY K DGF+S  EM++  NAVI
Sbjct: 671  LINSQRSELLRLVLREKNSNIPRACKDLFWKMIKVLHLFYLKDDGFTSN-EMISTANAVI 729

Query: 290  DEPISLPSSSSGKGMDGPCVA*EVAERIGQQF--------DSSWF 180
             EP++         +        + E I   F        D+SWF
Sbjct: 730  TEPVAFHGPFLSWLLSATIYVHSMLENINPLFDGLSYTVYDASWF 774


>gb|AEN74904.1| ent-kaurene synthase [Pyrus communis]
          Length = 737

 Score =  498 bits (1283), Expect = e-138
 Identities = 259/486 (53%), Positives = 339/486 (69%), Gaps = 4/486 (0%)
 Frame = -2

Query: 1718 VLDETYRCWLRREEEIFLDIETCAMAFRLLRTNGFVVSSDTLAEFNEEKDFFTSAGGNIK 1539
            VLDETYR WL+ +E+I  D  TCAMAFRLLR NG+ VS+D L +++E++  F S GG +K
Sbjct: 252  VLDETYRGWLQGDEDILSDAATCAMAFRLLRVNGYDVSADPLTQYSEDR-LFNSLGGYMK 310

Query: 1538 GIHTVLELYKTSQIMILPNEPVLEILNSWTSQLLKQNLANGVIHDGNCQK----EVVNSL 1371
             I   LEL++ S+ +I P+E VLE  + WTS  LKQ L+N +I      K    EV ++L
Sbjct: 311  DIDDALELFRASETIIHPDESVLEKQHHWTSHFLKQELSNTLIRGHKLNKHIGLEVDDAL 370

Query: 1370 KFTYDADVERLENRRNIECYNVDSLWVLKASYRCSNIDNRDIVQFGVEDYNFCQGILQKE 1191
            K    A + RL  R  I+ YN DS  +LK+ YRC NI N D ++  V+D N CQ I ++E
Sbjct: 371  KLPRYAILGRLSTRTAIKYYNTDSTRILKSYYRCLNIGNEDFLKLAVDDLNNCQSIHREE 430

Query: 1190 LANLERWVKENRLDQLKFARLKLEYLYFSVAGILFSPELSDARMSWTKNSFLSTVVDDFF 1011
            L +L RWV+E RLD+L FAR K  Y YFS A  LF PELSDAR+SW KN  L+TVVDDFF
Sbjct: 431  LNHLTRWVEEYRLDKLNFARQKQAYCYFSAAATLFPPELSDARISWAKNGVLTTVVDDFF 490

Query: 1010 DVKGSREELVNLINLVEKWERDTATDYSSEQVEIIFLAIHKTINDLGDIAFTRQGRNIER 831
            DV GS EEL+NLI LVEKW+ +   D  SE VEIIF A+  TIN++G  AFTRQGR++  
Sbjct: 491  DVGGSEEELINLIQLVEKWDVNVGVDCCSENVEIIFSALKNTINEIGARAFTRQGRSVTS 550

Query: 830  HMIDLWLSLLNSMMKEYEWVRDKSAPKVEEYIETASISFTLGPVVLVTQYFLGPKLSEEV 651
            H+I++WL L+ SM KE EW+R+KS P +EEY+E A +SF LGP+VL   Y +GPKLSEE 
Sbjct: 551  HVIEIWLDLIKSMFKEAEWLRNKSVPTMEEYMENAYVSFALGPIVLPALYLVGPKLSEEA 610

Query: 650  ITSPEYNDLFKHTNICCRLLNDVQTFKREGEQGKLNSVLLCKIHSPEAITEQQAVREIQS 471
            + S E++ L++  +   RLLND+Q FKRE  +GKLN+V L  IH    +TE++A+ E+++
Sbjct: 611  VGSSEFDHLYRLMSTAGRLLNDIQGFKRESAEGKLNAVSLAMIHG-NGVTEEEAINEMKN 669

Query: 470  IIDRNRRELLGLVLDTKGSIVPKACKDLFWNTFRVTHLFYRKKDGFSSPLEMVNAVNAVI 291
            +I   RRELL LVL  KGS+VP+ACKDLFWN  +V HLFY K DGF++  +M+  VNAV+
Sbjct: 670  VIVSKRRELLRLVLLEKGSVVPRACKDLFWNMSKVLHLFYAKHDGFTAH-DMMKTVNAVM 728

Query: 290  DEPISL 273
            +EPI L
Sbjct: 729  EEPILL 734