BLASTX nr result
ID: Coptis24_contig00013122
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00013122
(1528 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002283782.2| PREDICTED: E3 ubiquitin-protein ligase HERC2... 646 0.0
ref|XP_004148391.1| PREDICTED: E3 ubiquitin-protein ligase HERC2... 622 e-176
ref|XP_002324218.1| predicted protein [Populus trichocarpa] gi|2... 621 e-175
dbj|BAJ94465.1| predicted protein [Hordeum vulgare subsp. vulgar... 616 e-174
ref|XP_004162977.1| PREDICTED: E3 ubiquitin-protein ligase HERC2... 615 e-174
>ref|XP_002283782.2| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Vitis vinifera]
Length = 564
Score = 646 bits (1666), Expect = 0.0
Identities = 326/496 (65%), Positives = 375/496 (75%), Gaps = 5/496 (1%)
Frame = +3
Query: 54 GKLSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRVEFDKAQGDR 233
G L K++S+AAGEAH+L LTGDG VYSWGRGTFGRLGTG E DELFPV ++FD A+
Sbjct: 11 GNLRGKIVSVAAGEAHSLVLTGDGRVYSWGRGTFGRLGTGSEADELFPVPIKFDSAEKSD 70
Query: 234 PK---FVEIAAGAYHSLALQDDGAIWCWGYNIYGQLGFNLENSLVPRFLEGFLELGSPDS 404
K FV IAAGAYHS+AL DDG +WCWGYN + +N LVP LE FL LGS S
Sbjct: 71 SKGVKFVGIAAGAYHSVALADDGTVWCWGYN---SCILSDDNCLVPHLLELFLGLGSLGS 127
Query: 405 LRDESSIDSKKPLKVCSVKAGGMMSLAIDSLGALWIWGNYPPQNHSGDGEFSHISSSAPL 584
L DES +K PLKV +VKAGGMMSLAID+LG LW+WGN P ++ GEFS +SS P+
Sbjct: 128 LTDESDTKNKTPLKVNAVKAGGMMSLAIDNLGGLWMWGNVP-ESSPDKGEFSLVSSFTPI 186
Query: 585 PVWEFHGHTVVKVACGNEHVVALVSAEETFTGGDLICYSWGNNNHRQLGLGDRENRLRPE 764
PVW+FHGHTVVKVACGNEHVVALVS ET+ GGDLICYSWGNN H QLGLGD ENRLRPE
Sbjct: 187 PVWDFHGHTVVKVACGNEHVVALVSHGETYKGGDLICYSWGNNGHGQLGLGDTENRLRPE 246
Query: 765 IVKTFNRECPWEVYEVACGAFHTAVLTRKKIHGNMIDSSHLESLELGEKESICWTFGLGD 944
+VKTFN E WEVYEVACGAFHTA+LT KK + + ES+CWTFGLGD
Sbjct: 247 VVKTFNLESLWEVYEVACGAFHTALLTHKKRPSDTL-------------ESMCWTFGLGD 293
Query: 945 NGQLGHGTTNSTYLPEPVKDLPPDVFLISVDCGLFHTSVVSSAGDVWSWGMEKGLGLCPD 1124
NGQLGHGTT LPEPVKDLP FL+SVDCGLFHTSVVSSAG+VWSWGMEKGLGLCPD
Sbjct: 294 NGQLGHGTTKGISLPEPVKDLPEHRFLVSVDCGLFHTSVVSSAGEVWSWGMEKGLGLCPD 353
Query: 1125 ASFTGTDAGDALSPLWIPCNEYGSKFVDPLQVVCGAAHTIIVAHSGYGLWAWGRGRSGAL 1304
ASFTGTD GDA SPL YGSKF DP+QV CGAAHT++VA GY LW+WGRGRSG L
Sbjct: 354 ASFTGTDPGDAPSPLL-----YGSKFRDPVQVACGAAHTVLVADDGYKLWSWGRGRSGVL 408
Query: 1305 GSGNTADSYAPCSVMWPPLDEDFQDALE--IVGEEARIQDEEPRRLAEIDKKLSLAMEEI 1478
G+G T D +AP +V+WPPL EDF++ LE E +I++++P + E++K+LSLA EE+
Sbjct: 409 GNGKTVDCFAPSTVLWPPLTEDFKE-LESGTTSGEKKIENKDPEEVTEMEKRLSLATEEM 467
Query: 1479 *HLRSKLTLTERYVSI 1526
L SKL+ ERY SI
Sbjct: 468 KLLHSKLSTMERYASI 483
>ref|XP_004148391.1| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Cucumis sativus]
Length = 571
Score = 622 bits (1605), Expect = e-176
Identities = 302/493 (61%), Positives = 369/493 (74%), Gaps = 3/493 (0%)
Frame = +3
Query: 54 GKLSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRVEFDKAQGDR 233
GKLS KV++++AGEAHTL LTGDG VYSWGRG FGRLGTG E DELFPVRV+ + +
Sbjct: 11 GKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGE-EN 69
Query: 234 PKFVEIAAGAYHSLALQDDGAIWCWGYNIYGQLGFNLENSLVPRFLEGFLELGSPDSLRD 413
K V IAAG+YHSLAL DDG++WCWGYNIYGQLGF+ NS P L+ FLEL P+SL +
Sbjct: 70 LKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTE 129
Query: 414 ESSIDSKKPLKVCSVKAGGMMSLAIDSLGALWIWGNYPPQNHSGDGEFSHISSSAPLPVW 593
ES SK LK+C +KAGG +S AID LGALW+WGN P + + S++SS P PVW
Sbjct: 130 ESGSKSKGSLKICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSSFTPSPVW 189
Query: 594 EFHGHTVVKVACGNEHVVALVSAEETFT-GGDLICYSWGNNNHRQLGLGDRENRLRPEIV 770
EFHGHTVVKVACGNEH+VALVS ET+ DL+CYSWG+N+H QLGLGD+++R P+I+
Sbjct: 190 EFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQII 249
Query: 771 KTFNRECPWEVYEVACGAFHTAVLTRKKIHGNMIDSSHLESLELGEKESICWTFGLGDNG 950
+TFN PW VYEVACGAFHTA+L+ K + + ES+CWTFGLG+NG
Sbjct: 250 ETFNEASPWAVYEVACGAFHTALLSHSKRPCDTL-------------ESVCWTFGLGENG 296
Query: 951 QLGHGTTNSTYLPEPVKDLPPDVFLISVDCGLFHTSVVSSAGDVWSWGMEKGLGLCPDAS 1130
QLGHGTT + +LP+PVK+LP V LISV CGLFHTSVVSS GD+WSWGME+GLGLCPDAS
Sbjct: 297 QLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDAS 356
Query: 1131 FTGTDAGDALSPLWIPCNEYGS-KFVDPLQVVCGAAHTIIVAHSGYGLWAWGRGRSGALG 1307
FTGT+ GDA+SPL I C+ S KF+DP+QV CGAAHT+IVAH GY LW+WGRGRSG LG
Sbjct: 357 FTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLG 416
Query: 1308 SGNTADSYAPCSVMWPPLDEDF-QDALEIVGEEARIQDEEPRRLAEIDKKLSLAMEEI*H 1484
+G D + P V+WPPL+EDF Q+ E EE ++++ + + E +KKL +AMEEI
Sbjct: 417 NGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKIEEVTEENKKLLMAMEEIKL 476
Query: 1485 LRSKLTLTERYVS 1523
L KL++ ERY S
Sbjct: 477 LLDKLSIMERYAS 489
>ref|XP_002324218.1| predicted protein [Populus trichocarpa] gi|222865652|gb|EEF02783.1|
predicted protein [Populus trichocarpa]
Length = 561
Score = 621 bits (1602), Expect = e-175
Identities = 309/488 (63%), Positives = 366/488 (75%), Gaps = 2/488 (0%)
Frame = +3
Query: 60 LSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRVEFDKAQGDRPK 239
LS KV+S+A GEAH+L LTGDG VYSWG+G FGRLGTG E DELFPV+V+F+ R
Sbjct: 7 LSGKVVSVATGEAHSLILTGDGRVYSWGKGMFGRLGTGSEVDELFPVQVKFETCVEKRLN 66
Query: 240 FVEIAAGAYHSLALQDDGAIWCWGYNIYGQLGFNLENSLVPRFLEGFLELGSPDSLRDES 419
FV +AAGAYHSLAL DDG++WCWGYNIYGQLG + EN +P ++ FLELGS SL D S
Sbjct: 67 FVGVAAGAYHSLALADDGSVWCWGYNIYGQLGVSGENYSMPHLMDQFLELGSLGSLIDHS 126
Query: 420 SIDSKKPLKVCSVKAGGMMSLAIDSLGALWIWGNYPPQNHSGDGEFSHISSSAPLPVWEF 599
S+ PLK+CS+KAGGMMSLAID GALW+WGN P Q + S SS P+PV +F
Sbjct: 127 ETKSEGPLKICSIKAGGMMSLAIDDHGALWMWGNCPQQRNKTGSSISFESSFIPIPVRDF 186
Query: 600 HGHTVVKVACGNEHVVALVSAEETFTGGDLICYSWGNNNHRQLGLGDRENRLRPEIVKTF 779
HGH+VVKVACG+EHVVALVSA ET+ DL+CYSWGNNNH QLGLGDRE+R+ PEIV+ F
Sbjct: 187 HGHSVVKVACGSEHVVALVSAGETYKVEDLVCYSWGNNNHGQLGLGDRESRVHPEIVEMF 246
Query: 780 NRECPWEVYEVACGAFHTAVLTRKKIHGNMIDSSHLESLELGEKESICWTFGLGDNGQLG 959
N++ W+VYEVACGA+HTA+LTRK+ + ES+CWTFGLGDNGQLG
Sbjct: 247 NKDSSWDVYEVACGAYHTALLTRKR-------------RTVDPLESVCWTFGLGDNGQLG 293
Query: 960 HGTTNSTYLPEPVKDLPPDVFLISVDCGLFHTSVVSSAGDVWSWGMEKGLGLCPDASFTG 1139
HGTT S PEPVK+LP LISVDCGLFHTSVVSS GDVWSWGMEKGLGLCPDASFTG
Sbjct: 294 HGTTQSVLKPEPVKELPGQAHLISVDCGLFHTSVVSSVGDVWSWGMEKGLGLCPDASFTG 353
Query: 1140 TDAGDALSPLWIP-CNEYGSKFVDPLQVVCGAAHTIIVAHSGYGLWAWGRGRSGALGSGN 1316
TDAGDA SPL + G F +P+QV CGAAHT++VA+ GY LW+WGRG SG LG+G
Sbjct: 354 TDAGDAASPLLMSGYGVDGPIFHNPIQVACGAAHTVLVANDGYKLWSWGRGTSGVLGNGK 413
Query: 1317 TADSYAPCSVMWPPLDEDF-QDALEIVGEEARIQDEEPRRLAEIDKKLSLAMEEI*HLRS 1493
T + +AP V+WPPL EDF Q + VGEE+ + +AE DK+LSLAMEEI L+S
Sbjct: 414 TINCFAPSFVLWPPLTEDFKQPEPKTVGEES----NGSKAVAETDKRLSLAMEEIELLQS 469
Query: 1494 KLTLTERY 1517
KL++ E+Y
Sbjct: 470 KLSIMEQY 477
>dbj|BAJ94465.1| predicted protein [Hordeum vulgare subsp. vulgare]
gi|326532960|dbj|BAJ89325.1| predicted protein [Hordeum
vulgare subsp. vulgare]
Length = 566
Score = 616 bits (1588), Expect = e-174
Identities = 310/494 (62%), Positives = 366/494 (74%), Gaps = 8/494 (1%)
Frame = +3
Query: 69 KVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRV----EFDKAQGDRP 236
KV ++AAGEAHTLALTGDG VYSWGRGTFGRLGTG+E D P V A RP
Sbjct: 4 KVSAVAAGEAHTLALTGDGEVYSWGRGTFGRLGTGREADVHVPTAVLPAVAAAAAGRPRP 63
Query: 237 KFVEIAAGAYHSLALQDDGAIWCWGYNIYGQLGFNLENSLVPRFLEGFLELGSPDSLRDE 416
+FV +AAGAYHSLAL D+G++W WGYNIYGQLG+ ENSL P ++GF +LGSP++L+DE
Sbjct: 64 RFVAVAAGAYHSLALDDEGSLWSWGYNIYGQLGYGEENSLSPCLVDGFQDLGSPETLQDE 123
Query: 417 SSID-SKKPLKVCSVKAGGMMSLAIDSLGALWIWGNYPPQNHSGDGEFSHISSSAPLPVW 593
+ ++ PLK+ SVKAGGMMS AIDSLGALW+WGN P Q + DGEF ++SAPLPVW
Sbjct: 124 AQDTLARTPLKLSSVKAGGMMSFAIDSLGALWMWGNCPQQ--TDDGEFYIAATSAPLPVW 181
Query: 594 EFHGHTVVKVACGNEHVVALVSAEETFTGGDLICYSWGNNNHRQLGLGDRENRLRPEIVK 773
+FHGHTVVKVACGNEHVVA VSA ET+TGGDL+CYSWGNNNH QLGLGD+E+R RP ++
Sbjct: 182 DFHGHTVVKVACGNEHVVAAVSAGETYTGGDLVCYSWGNNNHGQLGLGDKESRSRPMLIP 241
Query: 774 TFNRECPWEVYEVACGAFHTAVLTRKKIHGNMIDSSHLESLELGEKESICWTFGLGDNGQ 953
F WEVYE+ACGA+HTAVLT KK + ES CWTFG+GDNGQ
Sbjct: 242 EFGEGSSWEVYEIACGAWHTAVLTNKKSFDQ-------------DLESRCWTFGIGDNGQ 288
Query: 954 LGHGTTNSTYLPEPVKDLPPDVFLISVDCGLFHTSVVSSAGDVWSWGMEKGLGLCPDASF 1133
LGHGTT +T P+PV LP FLIS+DCGLFHT+VVSS G+VW WGME+GLGLCPDASF
Sbjct: 289 LGHGTTATTCSPQPVDGLPTGSFLISLDCGLFHTAVVSSDGEVWCWGMERGLGLCPDASF 348
Query: 1134 TGTDAGDALSPLWIPCNE-YGSKFVDPLQVVCGAAHTIIVAHSGYGLWAWGRGRSGALGS 1310
+G DAGDAL P+ + E G KF+ P+QV CGAAHT++VA GY +WAWGRGRSG LG
Sbjct: 349 SGMDAGDALHPIRVQSPETNGFKFLGPVQVACGAAHTVLVAGDGYRMWAWGRGRSGVLGR 408
Query: 1311 GNTADSYAPCSVMWPPLDEDFQDALEIVGEEARIQDE--EPRRLAEIDKKLSLAMEEI*H 1484
T DSY PC VMWPPLDE+FQ EI G++A+ R E++ KLS A EE+
Sbjct: 409 DQTTDSYTPCVVMWPPLDENFQ---EIHGDQAQASTSRANDRTNTELELKLSAASEELQF 465
Query: 1485 LRSKLTLTERYVSI 1526
LRSKLTL ERY +I
Sbjct: 466 LRSKLTLMERYANI 479
>ref|XP_004162977.1| PREDICTED: E3 ubiquitin-protein ligase HERC2-like [Cucumis sativus]
Length = 581
Score = 615 bits (1587), Expect = e-174
Identities = 303/503 (60%), Positives = 371/503 (73%), Gaps = 13/503 (2%)
Frame = +3
Query: 54 GKLSQKVISLAAGEAHTLALTGDGCVYSWGRGTFGRLGTGKEDDELFPVRVEFDKAQGDR 233
GKLS KV++++AGEAHTL LTGDG VYSWGRG FGRLGTG E DELFPVRV+ + +
Sbjct: 11 GKLSGKVVAISAGEAHTLVLTGDGRVYSWGRGMFGRLGTGAESDELFPVRVKLGSGE-EN 69
Query: 234 PKFVEIAAGAYHSLALQDDGAIWCWGYNIYGQLGFNLENSLVPRFLEGFLELGSPDSLRD 413
K V IAAG+YHSLAL DDG++WCWGYNIYGQLGF+ NS P L+ FLEL P+SL +
Sbjct: 70 LKVVAIAAGSYHSLALTDDGSVWCWGYNIYGQLGFDGGNSATPCLLKQFLELEPPESLTE 129
Query: 414 ESSIDSKKPLKVCSVKAGGMMSLAIDSLGALWIWGNYPPQNHSGDGEFSHISSSAPLPVW 593
ES SK LK+C +KAGG +S AID LGALW+WGN P + + S++SS P PVW
Sbjct: 130 ESGSKSKGSLKICDIKAGGQISFAIDDLGALWMWGNCPKPSGENEVGLSYVSSFTPSPVW 189
Query: 594 EFHGHTVVKVACGNEHVVALVSAEETFT-GGDLICYSWGNNNHRQLGLGDRENRLRPEIV 770
EFHGHTVVKVACGNEH+VALVS ET+ DL+CYSWG+N+H QLGLGD+++R P+I+
Sbjct: 190 EFHGHTVVKVACGNEHIVALVSVGETYNKAEDLVCYSWGDNSHGQLGLGDKDSRACPQII 249
Query: 771 KTFNRECPWEVYEVACGAFHTAVLTRKKIHGNMIDSSHLESLELGEKESICWTFGLGDNG 950
+TFN PW VYEVACGAFHTA+L+ K + + ES+CWTFGLG+NG
Sbjct: 250 ETFNEASPWAVYEVACGAFHTALLSHSKRPCDTL-------------ESVCWTFGLGENG 296
Query: 951 QLGHGTTNSTYLPEPVKDLPPDVFLISVDCGLFHTSVVSSAGDVWSWGMEKGLGLCPDAS 1130
QLGHGTT + +LP+PVK+LP V LISV CGLFHTSVVSS GD+WSWGME+GLGLCPDAS
Sbjct: 297 QLGHGTTQNEFLPQPVKELPQSVSLISVACGLFHTSVVSSGGDLWSWGMERGLGLCPDAS 356
Query: 1131 FTGTDAGDALSPLWIPCNEYGS-KFVDPLQVVCGAAHTIIVAHSGYGLWAWGRGRSGALG 1307
FTGT+ GDA+SPL I C+ S KF+DP+QV CGAAHT+IVAH GY LW+WGRGRSG LG
Sbjct: 357 FTGTNTGDAISPLSIFCSGTESPKFLDPVQVACGAAHTVIVAHDGYKLWSWGRGRSGVLG 416
Query: 1308 SGNTADSYAPCSVMWPPLDEDF-QDALEIVGEEARIQDE-------EPRRLAEI---DKK 1454
+G D + P V+WPPL+EDF Q+ E EE ++++ E ++ E+ +KK
Sbjct: 417 NGKAVDCFTPTVVLWPPLNEDFDQEETETKCEEDKVEENKTEEDKVEENKIEEVTEENKK 476
Query: 1455 LSLAMEEI*HLRSKLTLTERYVS 1523
L +AMEEI L KL++ ERY S
Sbjct: 477 LLMAMEEIKLLLDKLSIMERYAS 499