BLASTX nr result
ID: Coptis24_contig00012409
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00012409
(1484 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum] 374 e-101
gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum] 373 e-101
gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclau... 300 8e-79
gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Pap... 298 3e-78
gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methylt... 294 4e-77
>gb|AFK73710.1| narcotoline-O-methyltransferase [Papaver somniferum]
Length = 356
Score = 374 bits (959), Expect = e-101
Identities = 184/349 (52%), Positives = 254/349 (72%), Gaps = 4/349 (1%)
Frame = -3
Query: 1395 DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDTKPIA----LSSLATSPTLVSI 1228
+MK +QI N +CG VD+ VL+CA++L +FD I N+ KP+ LSS+ +SP+ SI
Sbjct: 11 EMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNSG--KPMITLTELSSIVSSPSSSSI 68
Query: 1227 KPNNLYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLLRGQNRSLVDWALAIIDETVID 1048
+P NLYRL+RYL M+L++I N+ T SLT SKLLLR Q +SL+DW LAI E ++
Sbjct: 69 EPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLAIXCEMMVV 128
Query: 1047 GWHELSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCC 868
WHELS ++P P F++VHGK+ ELAGE N +IN+AM SDT + P +Q C
Sbjct: 129 VWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGC 188
Query: 867 DKLLNGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFK 688
K+LNG+TS++D+GGG G TM+YIV+AFPHIK V DLPHV+ ++ + PGVE + GD+FK
Sbjct: 189 GKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFK 248
Query: 687 FIPPADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAK 508
I ADA+ LK++LHNW D EC+ +LK+CKE +P DKGKVII+D+V D ++ + LT+AK
Sbjct: 249 SISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVID-DDDNSILTQAK 307
Query: 507 MNLDIDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 361
++LD+ +M GGRERT+ +W L++++GFSRHEIIPI A+ S+IVAYP
Sbjct: 308 LSLDLTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356
>gb|AFB74612.1| O-methyltransferase 2 [Papaver somniferum]
Length = 356
Score = 373 bits (958), Expect = e-101
Identities = 184/349 (52%), Positives = 254/349 (72%), Gaps = 4/349 (1%)
Frame = -3
Query: 1395 DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDTKPIA----LSSLATSPTLVSI 1228
+MK +QI N +CG VD+ VL+CA++L +FD I N+ KP+ LSS+ +SP+ SI
Sbjct: 11 EMKYQSQIWNQICGTVDTSVLRCAIQLGIFDAIHNSG--KPMITLTELSSIVSSPSSSSI 68
Query: 1227 KPNNLYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLLRGQNRSLVDWALAIIDETVID 1048
+P NLYRL+RYL M+L++I N+ T SLT SKLLLR Q +SL+DW LAI E ++
Sbjct: 69 EPCNLYRLVRYLSQMDLISIGECLNEATVSLTGTSKLLLRNQEKSLIDWVLAISCEMMVV 128
Query: 1047 GWHELSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCC 868
WHELS ++P P F++VHGK+ ELAGE N +IN+AM SDT + P +Q C
Sbjct: 129 VWHELSSSVSTPADEPPIFQKVHGKNALELAGEFPEWNDLINNAMTSDTSVTKPALIQGC 188
Query: 867 DKLLNGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFK 688
K+LNG+TS++D+GGG G TM+YIV+AFPHIK V DLPHV+ ++ + PGVE + GD+FK
Sbjct: 189 GKILNGVTSLIDVGGGHGATMAYIVEAFPHIKGAVIDLPHVVEAAPERPGVEFISGDIFK 248
Query: 687 FIPPADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAK 508
I ADA+ LK++LHNW D EC+ +LK+CKE +P DKGKVII+D+V D ++ + LT+AK
Sbjct: 249 SISNADAVLLKYVLHNWEDTECVNLLKRCKEAVPADKGKVIIMDLVID-DDDNSILTQAK 307
Query: 507 MNLDIDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 361
++LD+ +M GGRERT+ +W L++++GFSRHEIIPI A+ S+IVAYP
Sbjct: 308 LSLDLTVMNHGGGRERTKEDWRNLIEMSGFSRHEIIPISAMPSIIVAYP 356
>gb|AAP45314.1| S-adenosyl-L-methionine:3'-hydroxy-N-methylcoclaurine
4'-O-methyltransferase 2 [Papaver somniferum]
Length = 357
Score = 300 bits (767), Expect = 8e-79
Identities = 152/346 (43%), Positives = 222/346 (64%), Gaps = 2/346 (0%)
Frame = -3
Query: 1392 MKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDTKPIALSSLATSPTLVSIKPNNL 1213
+K AQ+ N + G DS+VL+CAVE+ + D+I NN I L+ LA + ++ + L
Sbjct: 17 IKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGA--ITLAQLAAKLPITNVSSDYL 74
Query: 1212 YRLLRYLVHMNLLTIHV--EGNDETFSLTELSKLLLRGQNRSLVDWALAIIDETVIDGWH 1039
YR++RYLVH+N++ G ++ +SL + LLLR RS+V L + + + WH
Sbjct: 75 YRMVRYLVHLNIIEQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMVSWH 134
Query: 1038 ELSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKL 859
+ + G T FE+ G +W+ N +Q+ N+ M +T L+ ++ C
Sbjct: 135 FMKEGLGN--GSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLTKTLIEDCRDT 192
Query: 858 LNGITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFKFIP 679
G+ S+VDIGGG G T+ I +AFPHIKCT++DLPHV+A+S LP +E V GDMFK +P
Sbjct: 193 FQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKSVP 252
Query: 678 PADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMNL 499
A AI LK +LH+W D+EC+ ILKKCKE IP++ GKVII+D+ ++ E + +LT+ ++ L
Sbjct: 253 SAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEE-ESNHELTKTRLIL 311
Query: 498 DIDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 361
DIDM+V +GGRERT ++WE LLK AGF H+I PI A+QSVI A+P
Sbjct: 312 DIDMLVNTGGRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357
>gb|ACO90232.1| putative norcoclaurine 6-O-methyltransferase [Papaver bracteatum]
Length = 346
Score = 298 bits (762), Expect = 3e-78
Identities = 156/347 (44%), Positives = 221/347 (63%), Gaps = 2/347 (0%)
Frame = -3
Query: 1395 DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDTKPIALSSLATS-PTLVSIKPN 1219
D ++ A+I + G +S+VLKCAV+L + + + NN KP++LS LA+ P + +
Sbjct: 8 DQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNN--VKPMSLSELASKLPVPQPVNED 65
Query: 1218 NLYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLLRGQNRSLVDWALAIIDETVIDGWH 1039
L+R++RYLVHM L I + + +SL +K LLRG +S+VD L I D+ + WH
Sbjct: 66 RLFRIMRYLVHMELFKI--DATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWH 123
Query: 1038 ELSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKL 859
L TG FE+ GKS+W EN NQ+ N AM DT LV C +
Sbjct: 124 HLGD---GLTGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI 180
Query: 858 LN-GITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFKFI 682
+ GI ++VD+GGG G + I KAFP IKCT++DLPHVIA S ++P + + GDMFK I
Sbjct: 181 FSDGIKTLVDVGGGTGTAVKTISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240
Query: 681 PPADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMN 502
P ADAIF+K +LH+W+D+ECI ILK+CKE +P+D GKVII+D+V D + + ++
Sbjct: 241 PSADAIFMKCILHDWNDEECIQILKRCKEALPKD-GKVIIVDVVIDM-DSTHPYAKIRLT 298
Query: 501 LDIDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 361
LD+DMM+ +GG+ERT+ EW++L AGF+ H++ I AVQSVI AYP
Sbjct: 299 LDLDMMLNTGGKERTKEEWKILFDAAGFASHKVTQISAVQSVIEAYP 345
>gb|AAP45315.1| S-adenosyl-L-methionine:norcoclaurine 6-O-methyltransferase [Papaver
somniferum]
Length = 346
Score = 294 bits (753), Expect = 4e-77
Identities = 155/347 (44%), Positives = 219/347 (63%), Gaps = 2/347 (0%)
Frame = -3
Query: 1395 DMKSHAQILNHMCGIVDSVVLKCAVELNLFDVISNNKDTKPIALSSLATS-PTLVSIKPN 1219
D ++ A+I + G +S+VLKCAV+L + + + NN KP++LS LA+ P + +
Sbjct: 8 DQQNQAKIWKQIYGFAESLVLKCAVQLEIAETLHNN--VKPMSLSELASKLPVAQPVNED 65
Query: 1218 NLYRLLRYLVHMNLLTIHVEGNDETFSLTELSKLLLRGQNRSLVDWALAIIDETVIDGWH 1039
L+R++RYLVHM L I + + +SL +K LLRG +S+VD L I D+ + WH
Sbjct: 66 RLFRIMRYLVHMELFKI--DATTQKYSLAPPAKYLLRGWEKSMVDSILCINDKDFLAPWH 123
Query: 1038 ELSGCCTSPTGGPTPFERVHGKSVWELAGENAGMNQVINDAMVSDTILVMPVFVQCCDKL 859
L TG FE+ GKS+W EN NQ+ N AM DT LV C +
Sbjct: 124 HLGD---GLTGNCDAFEKALGKSIWVYMSENPEKNQLFNAAMACDTRLVTSALANECKSI 180
Query: 858 LN-GITSMVDIGGGVGMTMSYIVKAFPHIKCTVFDLPHVIASSAQLPGVEMVGGDMFKFI 682
+ GI+++VD+GGG G + I KAFP IKCT++DLPHVIA S ++P + + GDMFK I
Sbjct: 181 FSDGISTLVDVGGGTGTAVKAISKAFPDIKCTIYDLPHVIADSPEIPNITKISGDMFKSI 240
Query: 681 PPADAIFLKFMLHNWHDKECITILKKCKEVIPQDKGKVIILDIVTDQNEQDDDLTRAKMN 502
P ADAIF+K +LH+W+D ECI ILK+CKE +P+ GKVII+D+V D + + ++
Sbjct: 241 PSADAIFMKCILHDWNDDECIQILKRCKEALPK-VGKVIIVDVVIDM-DSTHPYAKIRLT 298
Query: 501 LDIDMMVTSGGRERTENEWEVLLKLAGFSRHEIIPIMAVQSVIVAYP 361
LD+DMM+ +GG+ERT+ EW+ L AGF+ H++ I AVQSVI AYP
Sbjct: 299 LDLDMMLNTGGKERTKEEWKTLFDAAGFASHKVTQISAVQSVIEAYP 345