BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00012358
         (836 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002516294.1| cytochrome P450, putative [Ricinus communis]...   300   3e-79
ref|XP_002281332.1| PREDICTED: secologanin synthase [Vitis vinif...   297   2e-78
ref|XP_003633108.1| PREDICTED: LOW QUALITY PROTEIN: secologanin ...   296   3e-78
gb|ABC69414.1| CYP72A57 [Nicotiana tabacum]                           288   8e-76
dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]       282   8e-74

>ref|XP_002516294.1| cytochrome P450, putative [Ricinus communis]
           gi|223544780|gb|EEF46296.1| cytochrome P450, putative
           [Ricinus communis]
          Length = 488

 Score =  300 bits (767), Expect = 3e-79
 Identities = 145/258 (56%), Positives = 191/258 (74%)
 Frame = -2

Query: 775 REAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTKPTLIILDPELLKEVLSDKHG 596
           ++AWSKP+NL H IV RV PF   TV+ YGK+S FW G  P LII DPEL+KEVLS+K G
Sbjct: 33  QQAWSKPVNLNHKIVQRVDPFTFNTVQKYGKISMFWNGKTPRLIISDPELMKEVLSNKLG 92

Query: 595 RIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQEKLKGMTPAFLTSCIELIEKW 416
            IQKPP NP I +L+ GL+ L+GEKW   R+LI+PAFH EKLKGM P F+ SC ++IE+W
Sbjct: 93  HIQKPPLNPLILILARGLTVLEGEKWAIHRRLINPAFHLEKLKGMVPVFVISCNKMIEEW 152

Query: 415 KKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKKIFELQKEQSILVMQAVQSVY 236
           KK    Q  CE+D W +IQ    D+ISR AFGSN+EEGKKIFELQ E  +L ++A+Q++Y
Sbjct: 153 KKMINHQETCEVDVWPEIQKVAKDIISRAAFGSNYEEGKKIFELQHELMMLTIEAMQTLY 212

Query: 235 IPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESGKSSVDDLLGMLLQYSRESSQ 56
           IPGFRF+PTKKN+RR  L+ EI   LRD++ RK  A+ +G+S VDDLL +LL  S ++S 
Sbjct: 213 IPGFRFVPTKKNRRRKKLNIEITSMLRDIVERKQNAMRTGQSKVDDLLSLLLHSSEQNSS 272

Query: 55  ADMNNLKTTGMSIDQVIE 2
            + +  ++ G++I++VIE
Sbjct: 273 GNESGRRSDGLTIEEVIE 290


>ref|XP_002281332.1| PREDICTED: secologanin synthase [Vitis vinifera]
           gi|296081714|emb|CBI20719.3| unnamed protein product
           [Vitis vinifera]
          Length = 512

 Score =  297 bits (760), Expect = 2e-78
 Identities = 145/258 (56%), Positives = 195/258 (75%), Gaps = 1/258 (0%)
 Frame = -2

Query: 835 IKGTPYKFLYGDLKELAGCFREAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTK 656
           I+GT YK +YGD+K L   F+EA SKP+ L H IVPRVIPF ++  + YGK+S  WI T+
Sbjct: 44  IRGTRYKPMYGDMKALKLSFQEAQSKPMTLNHSIVPRVIPFFHQMFQNYGKISMSWIFTR 103

Query: 655 PTLIILDPELLKEVLSDKHGRIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQE 476
           P ++I+DPEL++ +L+DK+G+ QKPP NP + LL+ GLS+L+GE+W K+RKLI+PAFH E
Sbjct: 104 PRVMIVDPELIRMILADKNGQFQKPPLNPLVDLLTLGLSTLEGEQWAKRRKLITPAFHVE 163

Query: 475 KLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKK 296
           KL GM PAF  SC  LIE+WK     QG  ELD   + Q+ T DVISR AFGS++EEGKK
Sbjct: 164 KLMGMVPAFSMSCCNLIERWKNWVGPQGTYELDVMPEFQNVTGDVISRAAFGSSYEEGKK 223

Query: 295 IFELQKEQSILVMQAVQSVYIPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESG 116
           +FELQKEQ++LV++A +++Y+PGFRF+PT KN+RR ++D EI   LR +I RK  A+++G
Sbjct: 224 VFELQKEQAVLVIEASRAIYLPGFRFVPTVKNRRRYHIDNEIKAMLRSMIDRKKQAMKNG 283

Query: 115 KSSV-DDLLGMLLQYSRE 65
            S   DDLLG+LLQ + E
Sbjct: 284 DSGYNDDLLGLLLQLTEE 301


>ref|XP_003633108.1| PREDICTED: LOW QUALITY PROTEIN: secologanin synthase-like [Vitis
           vinifera]
          Length = 520

 Score =  296 bits (759), Expect = 3e-78
 Identities = 146/279 (52%), Positives = 199/279 (71%), Gaps = 1/279 (0%)
 Frame = -2

Query: 835 IKGTPYKFLYGDLKELAGCFREAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTK 656
           I+GTPYK + GD+KE      EAWSKP+NL HHIV RV PF    ++ YGK+S FW GT 
Sbjct: 44  IRGTPYKLVMGDMKEYIRLITEAWSKPMNLNHHIVSRVDPFTQNNMQQYGKVSLFWAGTT 103

Query: 655 PTLIILDPELLKEVLSDKHGRIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQE 476
           P LI++DP ++KEVLS+K G  QKP  +P I  L+ GL++L+GE W K R++I+PAFH E
Sbjct: 104 PRLIVMDPGMIKEVLSNKQGHFQKPYISPLILTLARGLTALEGEVWAKXRRIINPAFHLE 163

Query: 475 KLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKK 296
           KLK M PAF TSC  LIE+WK+    Q  CE+D W ++Q+ T DVISR A GS+FEEG++
Sbjct: 164 KLKVMIPAFTTSCSMLIERWKELASLQETCEVDIWPELQNLTRDVISRAALGSSFEEGRQ 223

Query: 295 IFELQKEQSILVMQAVQSVYIPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESG 116
           IFELQKE   L ++A+Q++YIPGFRFIPTKKN+RR  L K      RDLI+RK  A+ +G
Sbjct: 224 IFELQKEHITLTLEAMQTLYIPGFRFIPTKKNQRRKYLQKRTTSMFRDLIQRKKDAIRTG 283

Query: 115 KSSVDDLLGMLLQYSRESS-QADMNNLKTTGMSIDQVIE 2
           ++  D+LLG+LL  S +++   ++ + K   +++++VIE
Sbjct: 284 QAEGDNLLGLLLLSSSQNNLPENVMSTKDNAITLEEVIE 322


>gb|ABC69414.1| CYP72A57 [Nicotiana tabacum]
          Length = 518

 Score =  288 bits (738), Expect = 8e-76
 Identities = 140/278 (50%), Positives = 204/278 (73%)
 Frame = -2

Query: 835 IKGTPYKFLYGDLKELAGCFREAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTK 656
           +KG  YK LYGD+KEL+G  +EA SKP+NL+  I PR++PF   T+K YGK SF W+G K
Sbjct: 46  LKGNSYKILYGDMKELSGMIKEANSKPMNLSDDIAPRLVPFFLDTIKKYGKKSFVWLGPK 105

Query: 655 PTLIILDPELLKEVLSDKHGRIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQE 476
           P ++I+DPEL+KE+ S K+   QKP  NP  KLL  GL SL+ +KW K RK+I+PAFH E
Sbjct: 106 PLVLIMDPELIKEIFS-KYYLYQKPHGNPVTKLLVQGLVSLEEDKWAKHRKIINPAFHLE 164

Query: 475 KLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKK 296
           KLK M PAF  SC E++ KW+     +G  E+D W  ++  ++DVISRTAFGSNFEEGK+
Sbjct: 165 KLKHMLPAFCLSCTEMLCKWEDIVSIKGSHEIDVWPHLEQLSSDVISRTAFGSNFEEGKR 224

Query: 295 IFELQKEQSILVMQAVQSVYIPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESG 116
           IFELQKEQ+   ++A++SVYIPG+RF+PTK+N+R   ++K++  ++R +I ++  A+++G
Sbjct: 225 IFELQKEQAQYFVEAIRSVYIPGWRFLPTKRNRRMKEVEKDVRASIRGIIDKRVKAMKAG 284

Query: 115 KSSVDDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIE 2
           ++S +DLLG+LL+     ++A+ +  K + MSI++VI+
Sbjct: 285 EASNEDLLGILLE--SNFTEAEQHRHKDSAMSIEEVIQ 320


>dbj|BAF98466.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 518

 Score =  282 bits (721), Expect = 8e-74
 Identities = 139/278 (50%), Positives = 201/278 (72%)
 Frame = -2

Query: 835 IKGTPYKFLYGDLKELAGCFREAWSKPINLTHHIVPRVIPFINRTVKIYGKMSFFWIGTK 656
           IKG  YKFL G+LK++    +EA +KP+ L+H IVPRV+PF+++TV+ YGK+SF W G  
Sbjct: 45  IKGPSYKFLSGNLKDIVSATQEARAKPMELSHKIVPRVMPFVHQTVEKYGKLSFIWYGPY 104

Query: 655 PTLIILDPELLKEVLSDKHGRIQKPPRNPHIKLLSNGLSSLQGEKWVKQRKLISPAFHQE 476
             + I+DP++++++LS+K G  +K   NP  KLL +G+ S +GEKWVK R++++PAFH E
Sbjct: 105 CRVNIMDPDMIRDILSNKFGHFEKIRTNPLGKLLISGVVSYEGEKWVKHRRILNPAFHLE 164

Query: 475 KLKGMTPAFLTSCIELIEKWKKKTYSQGYCELDAWHDIQDFTADVISRTAFGSNFEEGKK 296
           KLK M PA  TSC E++ KW+    SQ  CELD W  +Q+ TADVISRTAFGS++EEG++
Sbjct: 165 KLKMMLPAIYTSCSEMVNKWEMMV-SQDSCELDVWPHLQNLTADVISRTAFGSSYEEGRR 223

Query: 295 IFELQKEQSILVMQAVQSVYIPGFRFIPTKKNKRRMNLDKEIIVNLRDLIRRKTLALESG 116
           IF+LQ EQ+ LV++A+QS+YIPG RF+PTK+N R   +  E  V LRD++ ++  A++ G
Sbjct: 224 IFQLQSEQAGLVIKALQSIYIPGLRFLPTKRNNRMKEIYGEARVLLRDIVNKREKAMKIG 283

Query: 115 KSSVDDLLGMLLQYSRESSQADMNNLKTTGMSIDQVIE 2
           ++  DDLLG+L++ S      D  N K  GM+ID+VI+
Sbjct: 284 EAHNDDLLGLLME-SNFKEITDNVNSKNFGMTIDEVID 320