BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00012354
         (1419 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI38103.3| unnamed protein product [Vitis vinifera]              507   e-141
ref|XP_002264377.2| PREDICTED: LOW QUALITY PROTEIN: translation ...   500   e-139
ref|XP_002328528.1| predicted protein [Populus trichocarpa] gi|2...   462   e-127
ref|XP_002879527.1| eIF4-gamma/eIF5/eIF2-epsilon domain-containi...   458   e-126
ref|NP_181042.1| translation initiation factor eIF-2B epsilon su...   451   e-124

>emb|CBI38103.3| unnamed protein product [Vitis vinifera]
          Length = 730

 Score =  507 bits (1306), Expect = e-141
 Identities = 256/391 (65%), Positives = 313/391 (80%), Gaps = 11/391 (2%)
 Frame = +2

Query: 2    VVGKGTSIGDHSKISNSVIGEGCVIGSNVIIEGSYIWNNVTIQDDCKVRHAIVCDGVVIK 181
            ++G GT+IGD++KISNSVIGE C IGSNV IEGSYIW+NVTI+D C ++HAIVC+ V++K
Sbjct: 340  IIGNGTNIGDNTKISNSVIGERCTIGSNVSIEGSYIWDNVTIEDGCVLKHAIVCNDVIMK 399

Query: 182  SGAVVEPGVILSFKVIIGQQFVVPAYSKVSLLPQPIKQDSDEELEYAD-NAIVED--ELA 352
            SGAV+EPGVILSFKV+IGQ+FVVPAYSKVSLL QP KQDSDEELEYAD N+ VE+   + 
Sbjct: 400  SGAVLEPGVILSFKVVIGQEFVVPAYSKVSLLQQPTKQDSDEELEYADHNSGVEEIPSIT 459

Query: 353  TVLDMPNGQSTSKPS-----NSSQVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXX 517
            + +D  NG  T K       ++S+VG+ G+GYIWS+CEGGHEEEWRHSVAP PADK    
Sbjct: 460  STVDKLNGVLTCKLPETEAWSTSEVGSGGVGYIWSICEGGHEEEWRHSVAPTPADKLTEV 519

Query: 518  XXXXXXXXXXXXXXGSVLPTSGELIPDSDI---VNSDNDDCKEDSVDFEKEVEATFLRAV 688
                           +VL  SGEL+PDS+     + DN+D ++DSV FEKEVEATFLRAV
Sbjct: 520  MQATDDDLELTTQGSNVLLPSGELVPDSNSNISEDDDNEDSRDDSVYFEKEVEATFLRAV 579

Query: 689  HENIEQDHVIIEVNSLRLSYNMSTLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITK 868
            HEN+++DHVI+EVNSLRLSYNM++ DCAGA+FYSVM+ A+ETP  +NSEL  NV  VIT+
Sbjct: 580  HENVKEDHVILEVNSLRLSYNMTSADCAGALFYSVMKLALETPCNSNSELLKNVASVITR 639

Query: 869  WGGLLKHYLQTEDEEIEVVIKFEEMCVESTKEFAPLFAQILHLLYDKDILSEDAIISWAS 1048
            W  L+K YLQ+ DEEIEV++KFEEMC+ES KEF+ LFAQILH+LY+KDIL EDAI+ WAS
Sbjct: 640  WQKLIKSYLQSMDEEIEVILKFEEMCLESAKEFSALFAQILHILYEKDILQEDAILKWAS 699

Query: 1049 EKEGADESDKVFLKQSETFIQWLKEAPEEDD 1141
            EKEGA+ESD+VF+KQSE FIQWL EA EE+D
Sbjct: 700  EKEGAEESDRVFVKQSEKFIQWLNEASEEED 730


>ref|XP_002264377.2| PREDICTED: LOW QUALITY PROTEIN: translation initiation factor eIF-2B
            subunit epsilon-like [Vitis vinifera]
          Length = 458

 Score =  500 bits (1287), Expect = e-139
 Identities = 250/383 (65%), Positives = 306/383 (79%), Gaps = 3/383 (0%)
 Frame = +2

Query: 2    VVGKGTSIGDHSKISNSVIGEGCVIGSNVIIEGSYIWNNVTIQDDCKVRHAIVCDGVVIK 181
            ++G GT+IGD++KISNSVIGE C IGSNV IEGSYIW+NVTI+D C ++HAIVC+ V++K
Sbjct: 77   IIGNGTNIGDNTKISNSVIGERCTIGSNVSIEGSYIWDNVTIEDGCVLKHAIVCNDVIMK 136

Query: 182  SGAVVEPGVILSFKVIIGQQFVVPAYSKVSLLPQPIKQDSDEELEYADNAIVEDELATVL 361
            SGAV+EPGVILSFKV+IGQ+FVVPAYSKVSLL QP KQDSDEELEYAD+     +    L
Sbjct: 137  SGAVLEPGVILSFKVVIGQEFVVPAYSKVSLLQQPTKQDSDEELEYADHNKHPSKFIVGL 196

Query: 362  DMPNGQSTSKPSNSSQVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXXXXXXXXXX 541
            ++     T + + + QVG+ G+GYIWS+CEGGHEEEWRHSVAP PADK            
Sbjct: 197  NVSLHLLT-RVTITXQVGSGGVGYIWSICEGGHEEEWRHSVAPTPADKLTEVMQATDDDL 255

Query: 542  XXXXXXGSVLPTSGELIPDSDI---VNSDNDDCKEDSVDFEKEVEATFLRAVHENIEQDH 712
                   +VL  SGEL+PDS+     + DN+D ++DSV FEKEVEATFLRAVHEN+++DH
Sbjct: 256  ELTTQGSNVLLPSGELVPDSNSNISEDDDNEDSRDDSVYFEKEVEATFLRAVHENVKEDH 315

Query: 713  VIIEVNSLRLSYNMSTLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITKWGGLLKHY 892
            VI+EVNSLRLSYNM++ DCAGA+FYSVM+ A+ETP  +NSEL  NV  VIT+W  L+K Y
Sbjct: 316  VILEVNSLRLSYNMTSADCAGALFYSVMKLALETPCNSNSELLKNVASVITRWQKLIKSY 375

Query: 893  LQTEDEEIEVVIKFEEMCVESTKEFAPLFAQILHLLYDKDILSEDAIISWASEKEGADES 1072
            LQ+ DEEIEV++KFEEMC+ES KEF+ LFAQILH+LY+KDIL EDAI+ WASEKEGA+ES
Sbjct: 376  LQSMDEEIEVILKFEEMCLESAKEFSALFAQILHILYEKDILQEDAILKWASEKEGAEES 435

Query: 1073 DKVFLKQSETFIQWLKEAPEEDD 1141
            D+VF+KQSE FIQWL EA EE+D
Sbjct: 436  DRVFVKQSEKFIQWLNEASEEED 458


>ref|XP_002328528.1| predicted protein [Populus trichocarpa] gi|222838243|gb|EEE76608.1|
            predicted protein [Populus trichocarpa]
          Length = 718

 Score =  462 bits (1188), Expect = e-127
 Identities = 228/383 (59%), Positives = 290/383 (75%), Gaps = 3/383 (0%)
 Frame = +2

Query: 2    VVGKGTSIGDHSKISNSVIGEGCVIGSNVIIEGSYIWNNVTIQDDCKVRHAIVCDGVVIK 181
            V+GKGT IG++S ISNSVIG+GC IGSNV I GSYIW++VTI+D C +RHAI+CDGVVIK
Sbjct: 342  VIGKGTRIGNNSNISNSVIGKGCSIGSNVSITGSYIWDSVTIEDGCDIRHAIICDGVVIK 401

Query: 182  SGAVVEPGVILSFKVIIGQQFVVPAYSKVSLLPQPIKQDSDEELEYADNAIVEDELATVL 361
            SGA +EPGV+LSFKV+IGQQF+VP+YSKVSL  QP  +DSDEELEYADN+    +   + 
Sbjct: 402  SGAALEPGVVLSFKVVIGQQFIVPSYSKVSLYQQPTVEDSDEELEYADNSSGTVDSCKLW 461

Query: 362  DMPNGQSTSKPSNSSQVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXXXXXXXXXX 541
            +M +       + +SQ+G  G+G++WS+CEGGHEEEWRHSVAPIPADK            
Sbjct: 462  EMMS------ETPASQLGPGGVGHVWSICEGGHEEEWRHSVAPIPADKLAEATQSLEDDL 515

Query: 542  XXXXXXGSVLPTSGELIPDSDIVNSDNDDC---KEDSVDFEKEVEATFLRAVHENIEQDH 712
                  G+ L TSGEL P  +  +S++DD    ++DS+ +EKEVEATFLRAV+ENI+   
Sbjct: 516  EFLNLDGNALSTSGELKPGRNGTDSEDDDAEDSRDDSIYYEKEVEATFLRAVNENIKVPD 575

Query: 713  VIIEVNSLRLSYNMSTLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITKWGGLLKHY 892
            VI+E+NSLRLSYNM++ DCAGAIFY++M+ A+E PH    EL  N   +I  W  LLK Y
Sbjct: 576  VILEMNSLRLSYNMTSADCAGAIFYAMMKQALEIPHATAGELRKNAASIIDAWNNLLKFY 635

Query: 893  LQTEDEEIEVVIKFEEMCVESTKEFAPLFAQILHLLYDKDILSEDAIISWASEKEGADES 1072
             +  D++IEV++KFEEMC+ES KEF+P F+QILH+LYDKDIL EDAI+ WA EK+ A+ES
Sbjct: 636  SKEIDDQIEVIMKFEEMCLESVKEFSPHFSQILHILYDKDILEEDAILRWADEKKDAEES 695

Query: 1073 DKVFLKQSETFIQWLKEAPEEDD 1141
            DKVF+KQSE  IQWL+EA EE+D
Sbjct: 696  DKVFVKQSEKLIQWLREASEEED 718


>ref|XP_002879527.1| eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein [Arabidopsis
            lyrata subsp. lyrata] gi|297325366|gb|EFH55786.1|
            eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 726

 Score =  458 bits (1179), Expect = e-126
 Identities = 227/380 (59%), Positives = 281/380 (73%)
 Frame = +2

Query: 2    VVGKGTSIGDHSKISNSVIGEGCVIGSNVIIEGSYIWNNVTIQDDCKVRHAIVCDGVVIK 181
            V+G GT IG+  KISNSVIG GC IGSNV+IEGSYIWNNVTI+D C++R+AIVCDGV I+
Sbjct: 347  VIGYGTKIGNGDKISNSVIGNGCSIGSNVVIEGSYIWNNVTIEDGCEIRNAIVCDGVKIR 406

Query: 182  SGAVVEPGVILSFKVIIGQQFVVPAYSKVSLLPQPIKQDSDEELEYADNAIVEDELATVL 361
            +GAV++PGV+LSF V++G+ FVVPAYSKVSLL QP  +DSDEELEYAD++      +   
Sbjct: 407  AGAVLQPGVVLSFNVVVGRDFVVPAYSKVSLLQQPTTEDSDEELEYADSS------SGTA 460

Query: 362  DMPNGQSTSKPSNSSQVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXXXXXXXXXX 541
            D  +G +    S +S++G  G GYIW  CEG H+EEW+HSVAPIP DK            
Sbjct: 461  DHLSGLNLQMESKASELGPDGAGYIWEACEGAHDEEWKHSVAPIPKDKLFEITQAIDDDD 520

Query: 542  XXXXXXGSVLPTSGELIPDSDIVNSDNDDCKEDSVDFEKEVEATFLRAVHENIEQDHVII 721
                   SV+PTSGEL  D+D +N+D +D  +D   FEKEVE T LRAV ENI+ D V +
Sbjct: 521  TDDE---SVVPTSGELKSDADSINTDVNDPNDDYYYFEKEVEGTVLRAVEENIKVDLVTL 577

Query: 722  EVNSLRLSYNMSTLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITKWGGLLKHYLQT 901
            E+N LRLSYNM + DCAGA FYS+++ A++TPH + SELY N   +ITKW  LL  Y++ 
Sbjct: 578  EINGLRLSYNMESADCAGATFYSMIKLAVDTPHNSASELYKNAASIITKWKELLGFYVKK 637

Query: 902  EDEEIEVVIKFEEMCVESTKEFAPLFAQILHLLYDKDILSEDAIISWASEKEGADESDKV 1081
             DE+IEV++KFEEMC ES KE  PLF QILHLLYDKD+L EDA++ WA EK GADE+DKV
Sbjct: 638  IDEQIEVIMKFEEMCQESPKELGPLFTQILHLLYDKDVLQEDAVLRWAEEKAGADEADKV 697

Query: 1082 FLKQSETFIQWLKEAPEEDD 1141
            +LKQ ETFIQWLKEA EE+D
Sbjct: 698  YLKQCETFIQWLKEASEEED 717


>ref|NP_181042.1| translation initiation factor eIF-2B epsilon subunit [Arabidopsis
            thaliana] gi|3033392|gb|AAC12836.1| putative translation
            initiation factor eIF-2B epsilon subunit [Arabidopsis
            thaliana] gi|22655208|gb|AAM98194.1| putative translation
            initiation factor eIF-2B epsilon subunit [Arabidopsis
            thaliana] gi|34098787|gb|AAQ56776.1| At2g34970
            [Arabidopsis thaliana] gi|330253950|gb|AEC09044.1|
            translation initiation factor eIF-2B epsilon subunit
            [Arabidopsis thaliana]
          Length = 730

 Score =  451 bits (1159), Expect = e-124
 Identities = 224/380 (58%), Positives = 278/380 (73%)
 Frame = +2

Query: 2    VVGKGTSIGDHSKISNSVIGEGCVIGSNVIIEGSYIWNNVTIQDDCKVRHAIVCDGVVIK 181
            V+G GT IG   KI NSVIG GC IGSNV+IEGSYIWNNVTI+D C++R+AIVCDGV I+
Sbjct: 348  VIGYGTKIGHGDKIMNSVIGNGCSIGSNVVIEGSYIWNNVTIEDGCEIRNAIVCDGVKIR 407

Query: 182  SGAVVEPGVILSFKVIIGQQFVVPAYSKVSLLPQPIKQDSDEELEYADNAIVEDELATVL 361
            +GAV++PGV+LSF V++G+ FVVPAYSKVSLL QP  +DSDEELEYAD++      +   
Sbjct: 408  AGAVLQPGVVLSFNVVVGRDFVVPAYSKVSLLQQPTTEDSDEELEYADSS------SGTA 461

Query: 362  DMPNGQSTSKPSNSSQVGNSGLGYIWSVCEGGHEEEWRHSVAPIPADKXXXXXXXXXXXX 541
            D  +G +    S +S++G  G GYIW VCEG H+EEW+HSVAPIP DK            
Sbjct: 462  DHLSGLNLQMESKASELGPDGAGYIWEVCEGAHDEEWKHSVAPIPKDKLSEITQAIDDDD 521

Query: 542  XXXXXXGSVLPTSGELIPDSDIVNSDNDDCKEDSVDFEKEVEATFLRAVHENIEQDHVII 721
                   SV+PTSGEL  D+D +N+D +D  +D   FEKEVE T LRAV ENI+ D V +
Sbjct: 522  TDDE---SVVPTSGELKSDADSINTDVNDPNDDYYYFEKEVEGTVLRAVEENIKVDLVTM 578

Query: 722  EVNSLRLSYNMSTLDCAGAIFYSVMRSAIETPHVANSELYGNVVRVITKWGGLLKHYLQT 901
            E+N LRLS+NM + DCAGA F+S+++ A++TPH + SELY N   +ITKW  LL  Y + 
Sbjct: 579  EINGLRLSFNMESADCAGATFFSMIKLALDTPHNSGSELYKNAASIITKWKDLLGFYAKK 638

Query: 902  EDEEIEVVIKFEEMCVESTKEFAPLFAQILHLLYDKDILSEDAIISWASEKEGADESDKV 1081
             DE+IEV++KFEEMC ES KE  PLF QILHLLYDKD+L EDAI+ W  EK GADE+DKV
Sbjct: 639  IDEQIEVIMKFEEMCQESHKELGPLFTQILHLLYDKDVLQEDAILRWEEEKAGADEADKV 698

Query: 1082 FLKQSETFIQWLKEAPEEDD 1141
            +LKQ +TFIQWLKEA EE+D
Sbjct: 699  YLKQCDTFIQWLKEASEEED 718