BLASTX nr result
ID: Coptis24_contig00012076
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00012076
(1493 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberin... 436 e-120
sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberin... 429 e-118
sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberi... 426 e-117
sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydropro... 415 e-113
sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase;... 398 e-108
>sp|C3SBS8.1|TNMT_ESCCA RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=EcTNMT gi|226897702|gb|ACO90222.1|
tetrahydroprotoberberine N-methyltransferase
[Eschscholzia californica]
Length = 350
Score = 436 bits (1122), Expect = e-120
Identities = 199/345 (57%), Positives = 268/345 (77%)
Frame = +3
Query: 288 KLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFINSLKNMDISGGNT 467
+++ RL +G + +EEL++L+ Q D+R++WGYKPT+E+QLAF+L+FI LK M +SG
Sbjct: 7 EIMGRLMKGEIEDEELKKLIRHQWDRRIEWGYKPTHEKQLAFNLDFIKGLKEMVMSG-EI 65
Query: 468 EMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLYCERAQIKDGLTI 647
+ +N+E YE+P FL+ FGK +KQS CYFK ++ TIDEAE A H+LYCERAQIKDG T+
Sbjct: 66 DTMNKETYELPTAFLEAVFGKTVKQSCCYFKDENSTIDEAEEAAHELYCERAQIKDGQTV 125
Query: 648 LDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNVKVILADVTKYET 827
LDIGCGQG L+ YIA+++KNCHVTG+TNS Q N+IE+Q L L NV VI ADVTK++T
Sbjct: 126 LDIGCGQGGLVLYIAEKYKNCHVTGLTNSKAQANYIEQQAEKLELTNVDVIFADVTKFDT 185
Query: 828 EALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAEKFEALDSDDWYS 1007
+ YDR++++E +EHMKNIQLF+ K+S WM +D+LLFVDH+ H F FEALD DDWYS
Sbjct: 186 DKTYDRILVVETIEHMKNIQLFMKKLSTWMTEDSLLFVDHISHKTFNHNFEALDEDDWYS 245
Query: 1008 EYLFLKGSVTVIAASSLLYFQDDVSVLNHWILNGKHMARTHESWLKKLLNNRNAARDILK 1187
++F KG VT++++S+LLYFQDDVS L+HW++NG HMAR+ E+W KKL AAR+IL+
Sbjct: 246 GFIFPKGCVTILSSSTLLYFQDDVSALDHWVVNGMHMARSVEAWRKKLDETIEAAREILE 305
Query: 1188 VCLGTEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 1322
LG++EA + I H RTF + EQ+S NNGEEWMI+ +LF+KK
Sbjct: 306 PGLGSKEAVNQVITHIRTFCIGGYEQFSYNNGEEWMITQILFKKK 350
>sp|Q108P1.1|TNMT_PAPSO RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase;
Short=PsTNMT gi|67764091|gb|AAY79177.1|
S-adenosyl-L-methionine:(S)-tetrahydroprotoberberine-cis-
N-methyltransferase [Papaver somniferum]
Length = 358
Score = 429 bits (1104), Expect = e-118
Identities = 201/355 (56%), Positives = 270/355 (76%), Gaps = 1/355 (0%)
Frame = +3
Query: 261 EQPKKESMLKLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFINSLK 440
++ KKES + L RL +G + +EEL++L+ Q +KRL+WGYK +++EQL+F+L+FI SLK
Sbjct: 5 DEVKKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLK 64
Query: 441 NMDISGGNTEMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLYCER 620
M++SG E +N+E YE+P FL+ FGK +KQS CYF H+S TIDEAE A H+LYCER
Sbjct: 65 KMEMSG-EIETMNKETYELPSEFLEAVFGKTVKQSMCYFTHESATIDEAEEAAHELYCER 123
Query: 621 AQIKDGLTILDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNVKVI 800
AQIKDG T+LDIGCGQG L+ YIAQ++KNCHVTG+TNS Q N++ +Q L L NV I
Sbjct: 124 AQIKDGQTVLDIGCGQGGLVLYIAQKYKNCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAI 183
Query: 801 LADVTKYETEALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAEKFE 980
LADVT+YE++ YDR+++IEA+EHMKN+QLF+ K+S WM K++LLFVDHVCH FA FE
Sbjct: 184 LADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTKESLLFVDHVCHKTFAHFFE 243
Query: 981 ALDSDDWYSEYLFLKGSVTVIAASSLLYFQDDVSVLNHWILNGKHMARTHESWLKKLLNN 1160
A+D DDWYS ++F G T++AA+SLLYFQDDVSV++HW++NG HMAR+ + W K L N
Sbjct: 244 AVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKN 303
Query: 1161 RNAARDILKVCL-GTEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 1322
AA++IL L G+ E + H RTF + EQ+S NNG+EWM++ +LF+KK
Sbjct: 304 MEAAKEILLPGLGGSHETVNGVVTHIRTFCMGGYEQFSMNNGDEWMVAQLLFKKK 358
>sp|C3SBU5.1|TNMT1_PAPBR RecName: Full=(S)-tetrahydroprotoberberine N-methyltransferase 1;
Short=PbTNMT1 gi|226897732|gb|ACO90237.1|
tetrahydroprotoberberine N-methyltransferase [Papaver
bracteatum]
Length = 358
Score = 426 bits (1095), Expect = e-117
Identities = 199/355 (56%), Positives = 271/355 (76%), Gaps = 1/355 (0%)
Frame = +3
Query: 261 EQPKKESMLKLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFINSLK 440
++ KKES + L RL +G + +EEL++L+ Q +KRL+WGYK +++EQL+F+L+FI SLK
Sbjct: 5 DEVKKESAGETLGRLLKGEIKDEELKKLIKFQFEKRLQWGYKSSHQEQLSFNLDFIKSLK 64
Query: 441 NMDISGGNTEMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLYCER 620
M++SG E +N+E YE+P FL+ FGK +KQS CYFKH+S TIDEAE A H+LYCER
Sbjct: 65 KMEMSG-EIETMNKETYELPSEFLEAVFGKTVKQSMCYFKHESATIDEAEEAAHELYCER 123
Query: 621 AQIKDGLTILDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNVKVI 800
AQIKDG T+LDIGCGQG L+ YIA+++K CHVTG+TNS Q N++ +Q L L NV I
Sbjct: 124 AQIKDGQTVLDIGCGQGGLVLYIARKYKKCHVTGLTNSKAQVNYLLKQAEKLGLTNVDAI 183
Query: 801 LADVTKYETEALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAEKFE 980
LADVT+YE++ YDR+++IEA+EHMKN+QLF+ K+S WM +++LLFVDHVCH FA FE
Sbjct: 184 LADVTQYESDKTYDRLLMIEAIEHMKNLQLFMKKLSTWMTEESLLFVDHVCHKTFAHFFE 243
Query: 981 ALDSDDWYSEYLFLKGSVTVIAASSLLYFQDDVSVLNHWILNGKHMARTHESWLKKLLNN 1160
A+D DDWYS ++F G T++AA+SLLYFQDDVSV++HW++NG HMAR+ + W K L N
Sbjct: 244 AVDEDDWYSGFIFPPGCATILAANSLLYFQDDVSVVDHWVVNGMHMARSVDIWRKALDKN 303
Query: 1161 RNAARDILKVCL-GTEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 1322
AA++IL L G+ EA + H RTF + EQ+S N+G+EWM++ +LF+KK
Sbjct: 304 MEAAKEILLPGLGGSHEAVNGVVTHIRTFCMGGYEQFSMNDGDEWMVAQLLFKKK 358
>sp|C3SBU4.1|TNMT2_PAPBR RecName: Full=Probable (S)-tetrahydroprotoberberine
N-methyltransferase 2; Short=PbTNMT2
gi|226897730|gb|ACO90236.1| putative N-methyltransferase
[Papaver bracteatum]
Length = 358
Score = 415 bits (1066), Expect = e-113
Identities = 198/355 (55%), Positives = 262/355 (73%), Gaps = 1/355 (0%)
Frame = +3
Query: 261 EQPKKESMLKLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFINSLK 440
E+ KKES + L RL RG +++EEL++L+ QL+KRL+WGYK +++EQL+F+L+FINSLK
Sbjct: 5 EEVKKESAEETLGRLLRGEINDEELKKLIKYQLEKRLQWGYKSSHQEQLSFNLDFINSLK 64
Query: 441 NMDISGGNTEMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLYCER 620
M +SG E E+YE+P + +GK++K S CYFKH+S TIDEAE A H+LYCER
Sbjct: 65 KMGMSG-QVEAFTNEVYELPTECFEAAYGKSMKLSGCYFKHESSTIDEAEEASHELYCER 123
Query: 621 AQIKDGLTILDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNVKVI 800
AQIKDG T+LDIGCGQG L+ Y+AQ++KNCHVTG+TNS Q N+I +Q L L+NV VI
Sbjct: 124 AQIKDGQTVLDIGCGQGGLVLYVAQKYKNCHVTGLTNSKEQVNYILKQAEKLGLRNVDVI 183
Query: 801 LADVTKYETEALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAEKFE 980
LADVT+YE++ YDR+++I +EHMKN+QLF+ K+S WM +D+LLFVDH CH F FE
Sbjct: 184 LADVTQYESDKTYDRILVIGVVEHMKNMQLFIKKLSTWMAEDSLLFVDHSCHKTFNHFFE 243
Query: 981 ALDSDDWYSEYLFLKGSVTVIAASSLLYFQDDVSVLNHWILNGKHMARTHESWLKKLLNN 1160
ALD DDWYS Y+F G T ++A SLLYFQDDVSV++HW++NG H ART ++W KKL N
Sbjct: 244 ALDEDDWYSGYIFPPGCATFLSADSLLYFQDDVSVVDHWVVNGMHFARTVDAWRKKLDKN 303
Query: 1161 RNAARDILKVCL-GTEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 1322
A ++IL L G EA I H RT + Q+S N+G+EWM + +LF+KK
Sbjct: 304 MEAVKEILLPGLGGNHEAVNGVITHIRTCCVGGYVQFSLNDGDEWMNAQLLFKKK 358
>sp|C3SBW0.1|PNMT_THLFG RecName: Full=Pavine N-methyltransferase; Short=PfPavNMT
gi|226897760|gb|ACO90251.1| pavine N-methyltransferase
[Thalictrum flavum]
Length = 356
Score = 398 bits (1023), Expect = e-108
Identities = 186/357 (52%), Positives = 266/357 (74%)
Frame = +3
Query: 252 MDSEQPKKESMLKLLDRLDRGLVSNEELRELVTIQLDKRLKWGYKPTNEEQLAFHLEFIN 431
M+++Q KKE++ L+ R++ G VS+EE+R ++ IQ+ KRLKWGYKPT+E+QLA + F
Sbjct: 1 METKQTKKEAVANLIKRIEHGEVSDEEIRGMMKIQVQKRLKWGYKPTHEQQLAQLVTFAQ 60
Query: 432 SLKNMDISGGNTEMINRELYEVPVPFLKLTFGKALKQSACYFKHQSMTIDEAEAAMHDLY 611
SLK M+++ + ++ ELYE+P+PFL + GK LK S YFK +S T+DE+E M DLY
Sbjct: 61 SLKGMEMAE-EVDTLDAELYEIPLPFLHIMCGKTLKFSPGYFKDESTTLDESEVYMMDLY 119
Query: 612 CERAQIKDGLTILDIGCGQGALIFYIAQRFKNCHVTGITNSNTQKNFIEEQCRNLNLQNV 791
CERAQIKDG +ILD+GCG G+L ++AQ+++ C VTGITNS +QK FI +QC+ L+L NV
Sbjct: 120 CERAQIKDGQSILDLGCGHGSLTLHVAQKYRGCKVTGITNSVSQKEFIMDQCKKLDLSNV 179
Query: 792 KVILADVTKYETEALYDRVIIIEALEHMKNIQLFLSKISKWMQKDALLFVDHVCHNLFAE 971
++IL DVTK+ETE YDR+ + +EHMKN +LFL K+S W+ + LLFV+H CH +FA
Sbjct: 180 EIILEDVTKFETEITYDRIFAVALIEHMKNYELFLKKVSTWIAQYGLLFVEHHCHKVFAY 239
Query: 972 KFEALDSDDWYSEYLFLKGSVTVIAASSLLYFQDDVSVLNHWILNGKHMARTHESWLKKL 1151
++E LD DDWY+EY+F G++ + ++S LLYFQ+DVSV+NHW L+GKH + + WLK+L
Sbjct: 240 QYEPLDEDDWYTEYIFPSGTLVMSSSSILLYFQEDVSVVNHWTLSGKHPSLGFKQWLKRL 299
Query: 1152 LNNRNAARDILKVCLGTEEAAEKCINHFRTFFLAMSEQYSCNNGEEWMISHVLFRKK 1322
+N + ++I + G++E A K I ++R F +A S+ YS NNGEEWM+S VLF+KK
Sbjct: 300 DDNIDEVKEIFESFYGSKEKAMKFITYWRVFCIAHSQMYSTNNGEEWMLSQVLFKKK 356