BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00010649
         (2385 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI34716.3| unnamed protein product [Vitis vinifera]              558   e-156
ref|XP_002276758.1| PREDICTED: uncharacterized protein LOC100251...   556   e-156
emb|CAN71799.1| hypothetical protein VITISV_004395 [Vitis vinifera]   474   e-131
ref|XP_003525724.1| PREDICTED: uncharacterized protein LOC100784...   461   e-127
ref|XP_003549859.1| PREDICTED: uncharacterized protein LOC100777...   459   e-126

>emb|CBI34716.3| unnamed protein product [Vitis vinifera]
          Length = 738

 Score =  558 bits (1439), Expect = e-156
 Identities = 337/665 (50%), Positives = 386/665 (58%), Gaps = 13/665 (1%)
 Frame = +1

Query: 46   PVPKTHPSLSLCYKITNSSLFHNYSFT---MARPNQESIDMFINITAASEAVALQKLEEH 216
            P P  HP++          L +++      MA PNQE+ID FI+IT ASEAVAL+KLEEH
Sbjct: 85   PTPTFHPTIYSSANPPPKPLNNSHQIPLVPMATPNQEAIDTFISITGASEAVALRKLEEH 144

Query: 217  GGDLNEAVNSYFSEGDRRIMHAAPVXXXXXXXXXXXXXXXXXXXXXXSLRLPSVTQNLNP 396
            GGDLNEAVN++FSEGDR I     +                      S  L + T NLNP
Sbjct: 145  GGDLNEAVNAHFSEGDRNIARETSIAAPQSDFMDIDDPIPVESQGPASSLLSAAT-NLNP 203

Query: 397  FSLLDP----SFRRSFLEGSASADFTSGAPRVTHPREVREVPIEFKDENNTQSHSGPGPR 564
             SLLDP    S  RS  +  + +D T   P V+HPREVRE+PIEFKD N T   SG  P 
Sbjct: 204  LSLLDPLLDPSSNRSLFD--SDSDLTRRGPSVSHPREVREIPIEFKDGNETSHQSGSTPT 261

Query: 565  IEDVTETSYAGEPEIRGTVIVXXXXXXXTPTAPSTELYGQNER---LLGDHGHVGQLTPS 735
            IEDVT+T+ A EPEIRG V+         PTAPS +  GQN +    LGD  H G+  P 
Sbjct: 262  IEDVTDTAPAHEPEIRGAVMTVDEDDDDIPTAPSAQGAGQNAQRDDTLGDSSHDGRSRPI 321

Query: 736  APGLDTATDYGNDXXXXXXXXXXXXSKREVAEGFQNQQFGATIDSSGPSLPHRESHVEDT 915
             PG +   DY ND            SKREV EG+ NQQFGA  + S P    R SH+ED 
Sbjct: 322  VPGFNNLPDYANDIEEEMVRAAIEASKREVEEGYSNQQFGAHNELSNPGPQQRPSHLEDA 381

Query: 916  ELERAVSLSLKTAEQEKALREQSGIVGIDQNTVFVASDVEDQRNSTVPNGRQGVGSFEEG 1095
             L RAVSLSLKTAEQEKALREQ G VG  +      ++VE+ R    PNGRQG GS ++ 
Sbjct: 382  ALARAVSLSLKTAEQEKALREQGGEVGASELGADSPAEVEELRELPAPNGRQGFGSLDKV 441

Query: 1096 TLIQPKLEAGSSSVQDEAEDVEEQPLVXXXXXXXXXXXXXA---NEASEMGDXXXXXXGP 1266
            T  +PKLEAGSSS+QDEAED++EQPLV             +    EA E+          
Sbjct: 442  TSSRPKLEAGSSSIQDEAEDIDEQPLVRNRTRRMSSGSVESVKETEAIEVSPVSSPRR-- 499

Query: 1267 HDIGNDPRHNGVGFHSDEWGGISSEEHDEAVMLEAALFGGIPEGTAYRFAYPPQEQIQAG 1446
             ++G DP  NG  FHSDEWGGISSEEHDEAVMLEAALFGGIP+     F + P   +  G
Sbjct: 500  QNVGADPDPNGTAFHSDEWGGISSEEHDEAVMLEAALFGGIPD----HFGFGPHRFMHPG 555

Query: 1447 SDRGVYXXXXXXXXXXXLEAQRLLREQQDDEYLASLQADREKELKXXXXXXXXXXXXXXX 1626
             DR              L AQRL+REQQDD YLASLQAD+EKELK               
Sbjct: 556  LDRN--FPPIARPPSPSLAAQRLIREQQDDAYLASLQADQEKELKAKEEAEARRLQEQMA 613

Query: 1627 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVSLPVEPSLGDENAVTLLVRMPDGSRR 1806
                                              SLP EP   DENAVTLLVRMPDGSRR
Sbjct: 614  RETALEEERRREEESRRKLEEQQEFERQLAAKEASLPQEPGSDDENAVTLLVRMPDGSRR 673

Query: 1807 GRRFLKSDNLQSLFDFIDVGRVVKPGTYRLVRPYPRRTFSDGESKLSLSDLGLTSKQEAL 1986
            GRRFLKSD LQSLF+FIDVGR VKPGTYRLVRPYPRR FSDGES LSL++LGLTSKQEAL
Sbjct: 674  GRRFLKSDKLQSLFNFIDVGRAVKPGTYRLVRPYPRRAFSDGESTLSLNELGLTSKQEAL 733

Query: 1987 FLELI 2001
            FLELI
Sbjct: 734  FLELI 738


>ref|XP_002276758.1| PREDICTED: uncharacterized protein LOC100251323 [Vitis vinifera]
          Length = 624

 Score =  556 bits (1434), Expect = e-156
 Identities = 332/635 (52%), Positives = 376/635 (59%), Gaps = 10/635 (1%)
 Frame = +1

Query: 127  MARPNQESIDMFINITAASEAVALQKLEEHGGDLNEAVNSYFSEGDRRIMHAAPVXXXXX 306
            MA PNQE+ID FI+IT ASEAVAL+KLEEHGGDLNEAVN++FSEGDR I     +     
Sbjct: 1    MATPNQEAIDTFISITGASEAVALRKLEEHGGDLNEAVNAHFSEGDRNIARETSIAAPQS 60

Query: 307  XXXXXXXXXXXXXXXXXSLRLPSVTQNLNPFSLLDP----SFRRSFLEGSASADFTSGAP 474
                             S  L + T NLNP SLLDP    S  RS  +  + +D T   P
Sbjct: 61   DFMDIDDPIPVESQGPASSLLSAAT-NLNPLSLLDPLLDPSSNRSLFD--SDSDLTRRGP 117

Query: 475  RVTHPREVREVPIEFKDENNTQSHSGPGPRIEDVTETSYAGEPEIRGTVIVXXXXXXXTP 654
             V+HPREVRE+PIEFKD N T   SG  P IEDVT+T+ A EPEIRG V+         P
Sbjct: 118  SVSHPREVREIPIEFKDGNETSHQSGSTPTIEDVTDTAPAHEPEIRGAVMTVDEDDDDIP 177

Query: 655  TAPSTELYGQNER---LLGDHGHVGQLTPSAPGLDTATDYGNDXXXXXXXXXXXXSKREV 825
            TAPS +  GQN +    LGD  H G+  P  PG +   DY ND            SKREV
Sbjct: 178  TAPSAQGAGQNAQRDDTLGDSSHDGRSRPIVPGFNNLPDYANDIEEEMVRAAIEASKREV 237

Query: 826  AEGFQNQQFGATIDSSGPSLPHRESHVEDTELERAVSLSLKTAEQEKALREQSGIVGIDQ 1005
             EG+ NQQFGA  + S P    R SH+ED  L RAVSLSLKTAEQEKALREQ G VG  +
Sbjct: 238  EEGYSNQQFGAHNELSNPGPQQRPSHLEDAALARAVSLSLKTAEQEKALREQGGEVGASE 297

Query: 1006 NTVFVASDVEDQRNSTVPNGRQGVGSFEEGTLIQPKLEAGSSSVQDEAEDVEEQPLVXXX 1185
                  ++VE+ R    PNGRQG GS ++ T  +PKLEAGSSS+QDEAED++EQPLV   
Sbjct: 298  LGADSPAEVEELRELPAPNGRQGFGSLDKVTSSRPKLEAGSSSIQDEAEDIDEQPLVRNR 357

Query: 1186 XXXXXXXXXXA---NEASEMGDXXXXXXGPHDIGNDPRHNGVGFHSDEWGGISSEEHDEA 1356
                      +    EA E+           ++G DP  NG  FHSDEWGGISSEEHDEA
Sbjct: 358  TRRMSSGSVESVKETEAIEVSPVSSPRR--QNVGADPDPNGTAFHSDEWGGISSEEHDEA 415

Query: 1357 VMLEAALFGGIPEGTAYRFAYPPQEQIQAGSDRGVYXXXXXXXXXXXLEAQRLLREQQDD 1536
            VMLEAALFGGIP+     F + P   +  G DR              L AQRL+REQQDD
Sbjct: 416  VMLEAALFGGIPD----HFGFGPHRFMHPGLDRN--FPPIARPPSPSLAAQRLIREQQDD 469

Query: 1537 EYLASLQADREKELKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1716
             YLASLQAD+EKELK                                             
Sbjct: 470  AYLASLQADQEKELKAKEEAEARRLQEQMARETALEEERRREEESRRKLEEQQEFERQLA 529

Query: 1717 XXXVSLPVEPSLGDENAVTLLVRMPDGSRRGRRFLKSDNLQSLFDFIDVGRVVKPGTYRL 1896
                SLP EP   DENAVTLLVRMPDGSRRGRRFLKSD LQSLF+FIDVGR VKPGTYRL
Sbjct: 530  AKEASLPQEPGSDDENAVTLLVRMPDGSRRGRRFLKSDKLQSLFNFIDVGRAVKPGTYRL 589

Query: 1897 VRPYPRRTFSDGESKLSLSDLGLTSKQEALFLELI 2001
            VRPYPRR FSDGES LSL++LGLTSKQEALFLELI
Sbjct: 590  VRPYPRRAFSDGESTLSLNELGLTSKQEALFLELI 624


>emb|CAN71799.1| hypothetical protein VITISV_004395 [Vitis vinifera]
          Length = 588

 Score =  474 bits (1220), Expect = e-131
 Identities = 301/633 (47%), Positives = 343/633 (54%), Gaps = 8/633 (1%)
 Frame = +1

Query: 127  MARPNQESIDMFINITAASEAVALQKLEEHGGDLNEAVNSYFSEGDRRIMHAAPVXXXXX 306
            MA PNQE+ID FI+IT ASEAVAL+KLEEHGGDLNEAVN++FSEGDR I     +     
Sbjct: 1    MATPNQEAIDTFISITGASEAVALRKLEEHGGDLNEAVNAHFSEGDRNIARETSIAAPQS 60

Query: 307  XXXXXXXXXXXXXXXXXSLRLPSVTQNLNPFSLLDP----SFRRSFLEGSASADFTSGAP 474
                             S  L + T NLNP SLLDP    S  RS  +  + +D T   P
Sbjct: 61   DFMDIDDPIPVESQGPASSLLSAAT-NLNPLSLLDPLLDPSSNRSLFD--SDSDLTRRGP 117

Query: 475  RVTHPREVREVPIEFKDENNTQSHSGPGPRIEDVTETSYAGEPEIRGTVIVXXXXXXXTP 654
             V+HPRE                 SG  P IEDVT+T+ A  PEIRG V+         P
Sbjct: 118  SVSHPRE-----------------SGSTPTIEDVTDTAPAHGPEIRGAVMTVDEDDDDIP 160

Query: 655  TAPSTELYGQNER---LLGDHGHVGQLTPSAPGLDTATDYGNDXXXXXXXXXXXXSKREV 825
            TAPS +  GQN +    LGD  H G+  P  PG +   DY ND            SKREV
Sbjct: 161  TAPSAQGAGQNAQRDDTLGDSSHDGRSRPIVPGFNNLPDYANDIEEEMVRAAIEASKREV 220

Query: 826  AEGFQNQQFGATIDSSGPSLPHRESHVEDTELERAVSLSLKTAEQEKALREQSGIVGIDQ 1005
             EG+ NQQFGA  + S P    R SH+ED  L RAVSLSLKTAEQEKALREQ G VG  +
Sbjct: 221  EEGYSNQQFGAHNELSNPGPQQRPSHLEDAALARAVSLSLKTAEQEKALREQGGEVGASE 280

Query: 1006 NTVFVASDVEDQRNSTVPNGRQGVGSFEEGTLIQPKLEAGSSSVQDEAEDVEEQPLVXXX 1185
                  ++VE+ R    PNGRQG GS ++ T  +PKLEAGSSS+QDEAED++EQPLV   
Sbjct: 281  LGADSPAEVEELRELPAPNGRQGFGSLDKVTSSRPKLEAGSSSIQDEAEDIDEQPLVRNR 340

Query: 1186 XXXXXXXXXXA-NEASEMGDXXXXXXGPHDIGNDPRHNGVGFHSDEWGGISSEEHDEAVM 1362
                      +  E   +           ++G DP  NG  FHSDEWGGISSEEHDEAVM
Sbjct: 341  TRRMSSGSVESVKETXAIEVSPVSSPRRQNVGADPDPNGTAFHSDEWGGISSEEHDEAVM 400

Query: 1363 LEAALFGGIPEGTAYRFAYPPQEQIQAGSDRGVYXXXXXXXXXXXLEAQRLLREQQDDEY 1542
            LEAALFGGIP+     F + P   +  G DR              L AQRL+REQQDD Y
Sbjct: 401  LEAALFGGIPD----HFGFGPHRFMHPGLDRN--FPPIARPPSPSLAAQRLIREQQDDAY 454

Query: 1543 LASLQADREKELKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1722
            LASLQAD+EKELK                                               
Sbjct: 455  LASLQADQEKELKAKEEAEARRLQEQMARETALEEERRREEESRRKLEEQQEFERQLAAK 514

Query: 1723 XVSLPVEPSLGDENAVTLLVRMPDGSRRGRRFLKSDNLQSLFDFIDVGRVVKPGTYRLVR 1902
              SLP EP   DENAVTLLVRMPDGSRRGRRFLKSD LQ                   VR
Sbjct: 515  EASLPQEPGSDDENAVTLLVRMPDGSRRGRRFLKSDKLQ-------------------VR 555

Query: 1903 PYPRRTFSDGESKLSLSDLGLTSKQEALFLELI 2001
            PYPRR FSDGES LSL++LGLTSKQEALFLELI
Sbjct: 556  PYPRRAFSDGESTLSLNELGLTSKQEALFLELI 588


>ref|XP_003525724.1| PREDICTED: uncharacterized protein LOC100784059 [Glycine max]
          Length = 598

 Score =  461 bits (1186), Expect = e-127
 Identities = 290/633 (45%), Positives = 341/633 (53%), Gaps = 8/633 (1%)
 Frame = +1

Query: 127  MARPNQESIDMFINITAASEAVALQKLEEHGGDLNEAVNSYFSEGDRRIM---HAAPVXX 297
            MARPNQE+++ F++IT   EAVALQKLEEHGG+LNEAVN++F+EGDR +    H +    
Sbjct: 1    MARPNQEAVETFVSITGLPEAVALQKLEEHGGNLNEAVNAHFTEGDRNLTTGSHNSSAVF 60

Query: 298  XXXXXXXXXXXXXXXXXXXXSLRLPSVTQNLNPFSLLDPSFRRSFLEGSASADFTSGAPR 477
                                   LP+   N+NPFSLLDP+  R         D T  AP 
Sbjct: 61   PQDDFMDIDDEHDAEIPRRIPPLLPASAANVNPFSLLDPTIGRGIF--GTRFDSTVEAPF 118

Query: 478  VTHPREVREVPIEFKDENNTQSHSGPGPRIEDVTETSYAGEPEIRGTVIVXXXXXXXTPT 657
            VTHPREVRE+PIE KD + +   +G  P IEDVT    A  P+I GTVI+        P 
Sbjct: 119  VTHPREVREIPIEVKDGSQSTPQAGHVPSIEDVTGNVDAHGPDIHGTVIIDDEDDENIPP 178

Query: 658  APSTELYGQNERLLGDHGHVGQLTPSAPGLDTATDYGNDXXXXXXXXXXXXSKREVAEGF 837
            A       Q  ++L D        PSAP  +   DY ND            SKRE  E +
Sbjct: 179  AQIAHQDEQTHKILADTSLNSSARPSAPESENLPDYSNDIEEEMIRAAIEASKREAEENY 238

Query: 838  QNQQFGATIDSSGPSLPHRESHVEDTELERAVSLSLKTAEQEKALREQSGIVGIDQNTVF 1017
            +N + G  ID S      R+S++ED EL  AVSLSLKTAEQEKAL+ Q G  G    T  
Sbjct: 239  RNHKLGRQIDLSESGSQPRQSYLEDPELAHAVSLSLKTAEQEKALQVQGGDSG--SPTAG 296

Query: 1018 VASDVEDQRNSTVPNGRQGVGSFEEGTLIQPKLEAGSSSVQDEAEDVEEQPLVXXXXXXX 1197
             +   E        NGR               L+AGS S  DEAEDVEEQPLV       
Sbjct: 297  PSKSSEAGLGEMTSNGR---------------LQAGSLSFHDEAEDVEEQPLVRNRSRHT 341

Query: 1198 XXXXXXANEASEMGD-----XXXXXXGPHDIGNDPRHNGVGFHSDEWGGISSEEHDEAVM 1362
                   ++  E+ +              D  N P HNG  F  DEWGGISSEEHDEAVM
Sbjct: 342  SSGSAGLDKDVELAEASTLPSTATVTATQDTSN-PHHNGNSF-PDEWGGISSEEHDEAVM 399

Query: 1363 LEAALFGGIPEGTAYRFAYPPQEQIQAGSDRGVYXXXXXXXXXXXLEAQRLLREQQDDEY 1542
            LEAA+FGGIPEGT YR+ Y P E +Q   +RG             L AQRL+REQQDDEY
Sbjct: 400  LEAAMFGGIPEGTGYRYGYAPHEFMQ---NRGFNPRPQYRPPSPSLAAQRLIREQQDDEY 456

Query: 1543 LASLQADREKELKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1722
            LASLQADREKELK                                               
Sbjct: 457  LASLQADREKELK-----------AMEEAEAAREEERRIAEESRRKLQEEQELETKLAAK 505

Query: 1723 XVSLPVEPSLGDENAVTLLVRMPDGSRRGRRFLKSDNLQSLFDFIDVGRVVKPGTYRLVR 1902
             VSLP EPS  D+NAV L+V+MPDG+RRGRRFL+SD LQSLFD+ID+GRVVKPG YRLVR
Sbjct: 506  EVSLPPEPSSDDDNAVNLMVKMPDGNRRGRRFLRSDRLQSLFDYIDIGRVVKPGNYRLVR 565

Query: 1903 PYPRRTFSDGESKLSLSDLGLTSKQEALFLELI 2001
            PYPRR FSDGES  +L +LGLT+KQEALFLELI
Sbjct: 566  PYPRRAFSDGESAATLDELGLTNKQEALFLELI 598


>ref|XP_003549859.1| PREDICTED: uncharacterized protein LOC100777000 isoform 1 [Glycine
            max]
          Length = 597

 Score =  459 bits (1180), Expect = e-126
 Identities = 288/632 (45%), Positives = 343/632 (54%), Gaps = 7/632 (1%)
 Frame = +1

Query: 127  MARPNQESIDMFINITAASEAVALQKLEEHGGDLNEAVNSYFSEGDRRIMHAAPVXXXXX 306
            MARPNQE+++ F++IT  +EAVALQKLEEHGG+LNEAVN++FSEGDR +   +       
Sbjct: 1    MARPNQEAVETFVSITGLTEAVALQKLEEHGGNLNEAVNAHFSEGDRNLTTGSRNSSAVF 60

Query: 307  XXXXXXXXXXXXXXXXXSLR--LPSVTQNLNPFSLLDPSFRRSFLEGSASADFTSGAPRV 480
                              +   LP++  N+NPFSLLDP+  R         D T  AP V
Sbjct: 61   PQDDFMDIDDQHDAGIPRIPSLLPALAANVNPFSLLDPTIGRGIF--GTRFDSTIEAPFV 118

Query: 481  THPREVREVPIEFKDENNTQSHSGPGPRIEDVTETSYAGEPEIRGTVIVXXXXXXXTPTA 660
            THPREVRE+PIE KD + +   +G  P IEDVT T  A  P+I GTVI+        P A
Sbjct: 119  THPREVREIPIEVKDGSQSTPQAGHVPSIEDVTGTVDAHGPDIHGTVIIDDEDDDDIPPA 178

Query: 661  PSTELYGQNERLLGDHGHVGQLTPSAPGLDTATDYGNDXXXXXXXXXXXXSKREVAEGFQ 840
                   Q +++L D      + PSAP  +   DY ND            SK+E  E ++
Sbjct: 179  QIAHQDEQTQKILADTSLNSSVRPSAPESENLPDYSNDIEEEMIRAAIEASKQEAEENYR 238

Query: 841  NQQFGATIDSSGPSLPHRESHVEDTELERAVSLSLKTAEQEKALREQSGIVGIDQNTVFV 1020
            N +    ID S      R+S++ED EL  AVSLSLKTAEQEKALR   G  G    T   
Sbjct: 239  NHKLDRQIDLSESGSQPRQSYLEDPELAHAVSLSLKTAEQEKALRVHGGDSG--SPTAGP 296

Query: 1021 ASDVEDQRNSTVPNGRQGVGSFEEGTLIQPKLEAGSSSVQDEAEDVEEQPLVXXXXXXXX 1200
            +   E        NGR               L+AGS S  DEAEDVEEQPLV        
Sbjct: 297  SKSSEAGLGEVTSNGR---------------LQAGSLSFHDEAEDVEEQPLVRNRSRHMS 341

Query: 1201 XXXXXANEASEMGD-----XXXXXXGPHDIGNDPRHNGVGFHSDEWGGISSEEHDEAVML 1365
                   +  E+ +              D  N P HNG  F  DEWGGISSEEHDEAVML
Sbjct: 342  SGSTGLGKDVELAEASTLPSTATVTATQDSSN-PHHNGNSF-PDEWGGISSEEHDEAVML 399

Query: 1366 EAALFGGIPEGTAYRFAYPPQEQIQAGSDRGVYXXXXXXXXXXXLEAQRLLREQQDDEYL 1545
            EAA+FGGIPEGT Y + Y P E +Q   +RG             L AQRL+REQQDDEYL
Sbjct: 400  EAAMFGGIPEGTGYHYGYAPHEFMQ---NRGFNPRPQYRPPSPSLAAQRLIREQQDDEYL 456

Query: 1546 ASLQADREKELKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1725
            ASLQADREKELK                                                
Sbjct: 457  ASLQADREKELK-----------AMEEAEAAREEERQRAEDSRRKLQEEQELETKLAAKE 505

Query: 1726 VSLPVEPSLGDENAVTLLVRMPDGSRRGRRFLKSDNLQSLFDFIDVGRVVKPGTYRLVRP 1905
            VSLP EPS  D+NAV L+V+MPDG+RRGRRFL+SD LQSLFD+ID+GRVVKPG+YRLVRP
Sbjct: 506  VSLPPEPSSDDDNAVNLMVKMPDGNRRGRRFLRSDRLQSLFDYIDIGRVVKPGSYRLVRP 565

Query: 1906 YPRRTFSDGESKLSLSDLGLTSKQEALFLELI 2001
            YPRR FSDGES  +L +LGLT+KQEALFLELI
Sbjct: 566  YPRRAFSDGESAATLDELGLTNKQEALFLELI 597