BLASTX nr result
ID: Coptis24_contig00010628
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00010628
(1569 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chl... 385 0.0
gb|ACF17660.1| putative isopropylmalate synthase [Capsicum annuum] 382 0.0
gb|ABA26446.1| 2-isopropylmalate synthase 1 [Brassica insularis] 370 0.0
ref|XP_002530549.1| isopropylmalate synthase, putative [Ricinus ... 379 0.0
gb|AAW81741.1| Putative 2-isopropylmalate synthase [Brassica ole... 370 0.0
>ref|XP_002268942.2| PREDICTED: 2-isopropylmalate synthase 2, chloroplastic-like [Vitis
vinifera]
Length = 628
Score = 385 bits (990), Expect(3) = 0.0
Identities = 192/247 (77%), Positives = 219/247 (88%), Gaps = 1/247 (0%)
Frame = +3
Query: 516 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 695
ERAGNASLEEVVMA++CRGE+ LGGL+TGIN RHIV SKMVAE++GLQVQPHKAIVGAN
Sbjct: 324 ERAGNASLEEVVMALKCRGEKALGGLYTGINIRHIVKTSKMVAEYSGLQVQPHKAIVGAN 383
Query: 696 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 875
AFAHESGIHQDGMLK+K TYEI+SPEDIGL SN+SGIVLGKLSGRHAL+SRLLELGYE+
Sbjct: 384 AFAHESGIHQDGMLKHKETYEIISPEDIGLFRSNESGIVLGKLSGRHALKSRLLELGYEI 443
Query: 876 MA-ELDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 1052
ELD +FWRFK VAEKKK+L+D D+ AL+SDEIFQP+ W LGDLQV CGTLGLSTAT
Sbjct: 444 EGMELDDIFWRFKEVAEKKKNLTDVDVIALVSDEIFQPKVFWKLGDLQVTCGTLGLSTAT 503
Query: 1053 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 1232
VKLI DG++H+ CSVGTGPVD+AY+AID +VKE VTLLEY +N+VTEGIDAIA+T VLI
Sbjct: 504 VKLIDADGQKHVACSVGTGPVDSAYKAIDLLVKEQVTLLEYGLNAVTEGIDAIATTRVLI 563
Query: 1233 AGENGHS 1253
GE H+
Sbjct: 564 RGEKNHT 570
Score = 295 bits (754), Expect(3) = 0.0
Identities = 145/173 (83%), Positives = 159/173 (91%)
Frame = +2
Query: 2 DDGHVPVICCLARCNKADIDAAWEAVKHAKRPRIHTFIATSEIHLKYKLRKSRDEVIAIA 181
++GHVPVIC LARCN+ DI AWEAVK+AKRPRIHTFIATSEIH+KYKL+KS EVI IA
Sbjct: 152 ENGHVPVICGLARCNRNDISTAWEAVKYAKRPRIHTFIATSEIHMKYKLKKSEKEVIEIA 211
Query: 182 KEMVSYARSLGCNDVEFSPEDAGRSDREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFG 361
+EMV YARSLGC+DVEFSPEDAGRS+REFLYKIL EVIKAGATTLNIPDTVGYT+PNEFG
Sbjct: 212 REMVKYARSLGCDDVEFSPEDAGRSEREFLYKILGEVIKAGATTLNIPDTVGYTVPNEFG 271
Query: 362 KLIADIKLNTPGIDDVIISTHCQNDLGLSTANTIERVGAGARQLEVTINGIGE 520
+LIADIK NTPGI++VIISTHCQNDLGLSTANTI AGARQ+EVTINGIGE
Sbjct: 272 QLIADIKSNTPGIENVIISTHCQNDLGLSTANTIAGACAGARQVEVTINGIGE 324
Score = 65.9 bits (159), Expect(3) = 0.0
Identities = 33/51 (64%), Positives = 40/51 (78%)
Frame = +1
Query: 1249 THAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKMLDFSRTSKVSGSSN 1401
T+A+TG+TVHR+FSG GAGMDIVVSS Y+SALNKML F SK S++
Sbjct: 572 TNALTGETVHRSFSGTGAGMDIVVSSVRAYISALNKMLGFKDQSKPKVSAD 622
>gb|ACF17660.1| putative isopropylmalate synthase [Capsicum annuum]
Length = 602
Score = 382 bits (981), Expect(3) = 0.0
Identities = 188/247 (76%), Positives = 224/247 (90%), Gaps = 1/247 (0%)
Frame = +3
Query: 516 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 695
ERAGNASLEEVVMA++CRGE++LGGL+TGINT+HI+M+SKMV E+TGL VQPHKAIVGAN
Sbjct: 299 ERAGNASLEEVVMALKCRGEQVLGGLYTGINTQHILMSSKMVEEYTGLHVQPHKAIVGAN 358
Query: 696 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 875
AFAHESGIHQDGMLK+K TYEIMSPEDIGL+ +N+SGIVLGKLSGRHAL++++LELGYE+
Sbjct: 359 AFAHESGIHQDGMLKHKDTYEIMSPEDIGLNRANESGIVLGKLSGRHALQAKMLELGYEI 418
Query: 876 MA-ELDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 1052
ELD +FWRFK+VAEKKK ++D D+ AL+SDE+FQPQ +W L ++QV CG+LGLSTAT
Sbjct: 419 EGKELDDLFWRFKSVAEKKKKITDDDLIALMSDEVFQPQFVWQLQNVQVTCGSLGLSTAT 478
Query: 1053 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 1232
VKLI DG+EHI CSVGTGPVDAAY+A+D IVK VTLLEYS+N+VT+GIDAIAST VLI
Sbjct: 479 VKLIDADGKEHISCSVGTGPVDAAYKAVDLIVKIPVTLLEYSMNAVTQGIDAIASTRVLI 538
Query: 1233 AGENGHS 1253
GENGH+
Sbjct: 539 RGENGHT 545
Score = 287 bits (735), Expect(3) = 0.0
Identities = 140/173 (80%), Positives = 156/173 (90%)
Frame = +2
Query: 2 DDGHVPVICCLARCNKADIDAAWEAVKHAKRPRIHTFIATSEIHLKYKLRKSRDEVIAIA 181
++GHVPVIC LARCNK DID AWEAVK+AK+PRIHTFIATSEIH+K+KL+ SRDEVI A
Sbjct: 127 EEGHVPVICGLARCNKRDIDKAWEAVKYAKKPRIHTFIATSEIHMKFKLKMSRDEVIEKA 186
Query: 182 KEMVSYARSLGCNDVEFSPEDAGRSDREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFG 361
+ MV+YARS+GC DVEFSPEDAGRSD EFLY IL EVIKAGATTLNIPDTVGYT+P EFG
Sbjct: 187 RSMVAYARSIGCQDVEFSPEDAGRSDPEFLYHILGEVIKAGATTLNIPDTVGYTVPEEFG 246
Query: 362 KLIADIKLNTPGIDDVIISTHCQNDLGLSTANTIERVGAGARQLEVTINGIGE 520
+LIA+IK NTPG++DVIISTHCQNDLGLSTANT+ AGARQLEVTINGIGE
Sbjct: 247 QLIANIKANTPGVEDVIISTHCQNDLGLSTANTLAGACAGARQLEVTINGIGE 299
Score = 64.7 bits (156), Expect(3) = 0.0
Identities = 31/55 (56%), Positives = 41/55 (74%), Gaps = 4/55 (7%)
Frame = +1
Query: 1246 ATHAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKMLDF----SRTSKVSGSS 1398
+THA+TG+TVHRTFSG GA MDIV+SS Y+ ALNKM+ F ++ +K GS+
Sbjct: 546 STHALTGETVHRTFSGTGADMDIVISSVRAYIGALNKMMSFRKLLAKNTKAEGSA 600
>gb|ABA26446.1| 2-isopropylmalate synthase 1 [Brassica insularis]
Length = 623
Score = 370 bits (951), Expect(3) = 0.0
Identities = 181/247 (73%), Positives = 215/247 (87%), Gaps = 1/247 (0%)
Frame = +3
Query: 516 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 695
ERAGNASLEEVVMAI+CRG+ +LGGL+TGI+TRHIVM SKMV ++TG+Q QPHKAIVGAN
Sbjct: 319 ERAGNASLEEVVMAIKCRGDHVLGGLYTGIDTRHIVMTSKMVEDYTGMQTQPHKAIVGAN 378
Query: 696 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 875
AFAHESGIHQDGMLK+KGTYEI+ PE+IGL SND+GIVLGKLSGRHAL+ RL ELGY L
Sbjct: 379 AFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYVL 438
Query: 876 MAE-LDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 1052
E L +FWRFK+VAE+KK ++D DI AL+SDE+FQP+A+W L D+Q+ CGTLGLSTAT
Sbjct: 439 DDEQLSSIFWRFKSVAERKKRVTDADIIALVSDEVFQPEALWKLLDIQITCGTLGLSTAT 498
Query: 1053 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 1232
VKL DG+EH+ CS+GTGPVD+AY+A+D IVKE TLLEYS+N+VTEGIDAIA+T VLI
Sbjct: 499 VKLADADGKEHVACSMGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLI 558
Query: 1233 AGENGHS 1253
G N +S
Sbjct: 559 RGNNNYS 565
Score = 286 bits (732), Expect(3) = 0.0
Identities = 139/172 (80%), Positives = 158/172 (91%)
Frame = +2
Query: 5 DGHVPVICCLARCNKADIDAAWEAVKHAKRPRIHTFIATSEIHLKYKLRKSRDEVIAIAK 184
DG+VPVIC L+RCNK DI+ AWEAVK+AKRPRIHTFIATS+IHL+YKL+KS+DEVI IA+
Sbjct: 148 DGYVPVICGLSRCNKRDIETAWEAVKYAKRPRIHTFIATSDIHLEYKLKKSKDEVIEIAR 207
Query: 185 EMVSYARSLGCNDVEFSPEDAGRSDREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFGK 364
MV +ARSLGC DVEFSPEDAGRS+REFLY+IL EVIKAGATTLNIPDTVG TLP+EFG+
Sbjct: 208 NMVKFARSLGCEDVEFSPEDAGRSEREFLYQILGEVIKAGATTLNIPDTVGITLPSEFGQ 267
Query: 365 LIADIKLNTPGIDDVIISTHCQNDLGLSTANTIERVGAGARQLEVTINGIGE 520
LIADIK NTPGI++VIISTHCQNDLGLSTANT+ +GARQ+EVTINGIGE
Sbjct: 268 LIADIKANTPGIENVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGE 319
Score = 64.3 bits (155), Expect(3) = 0.0
Identities = 34/53 (64%), Positives = 40/53 (75%), Gaps = 2/53 (3%)
Frame = +1
Query: 1249 THAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKMLDF--SRTSKVSGSSN 1401
T+AITG+ V RTFSG GAGMDIVVSS YV ALNKMLDF + T+K+ +N
Sbjct: 567 TNAITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMLDFKENSTTKIPSQNN 619
>ref|XP_002530549.1| isopropylmalate synthase, putative [Ricinus communis]
gi|223529911|gb|EEF31840.1| isopropylmalate synthase,
putative [Ricinus communis]
Length = 632
Score = 379 bits (973), Expect(3) = 0.0
Identities = 189/247 (76%), Positives = 214/247 (86%), Gaps = 1/247 (0%)
Frame = +3
Query: 516 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 695
ERAGNASLEEVVMAI+CRGE +LGGL+TGIN R I MASKMV E+TGL +QPHKAIVGAN
Sbjct: 326 ERAGNASLEEVVMAIKCRGEHILGGLYTGINPRQITMASKMVEEYTGLHLQPHKAIVGAN 385
Query: 696 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 875
AFAHESGIHQDGMLK+KGTYEI+SPEDIGL SND+GIVLGKLSGRHAL+ RL ELGYEL
Sbjct: 386 AFAHESGIHQDGMLKHKGTYEIISPEDIGLERSNDAGIVLGKLSGRHALKDRLKELGYEL 445
Query: 876 MAE-LDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 1052
E L +FWRFKAVAE KK ++D D+ AL+SDE+FQP+ +W LGDLQV CGTLGLSTAT
Sbjct: 446 NDEQLGTIFWRFKAVAETKKRVTDADLIALVSDEVFQPEIVWKLGDLQVTCGTLGLSTAT 505
Query: 1053 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 1232
VKLI DGEEHI CSVG+GPVDA Y+A+D IVKE V LLEYS+N+VTEGIDAIA+T VLI
Sbjct: 506 VKLINADGEEHIACSVGSGPVDAGYKAVDLIVKEPVELLEYSMNAVTEGIDAIATTRVLI 565
Query: 1233 AGENGHS 1253
E+ H+
Sbjct: 566 RREDNHT 572
Score = 283 bits (725), Expect(3) = 0.0
Identities = 139/173 (80%), Positives = 156/173 (90%)
Frame = +2
Query: 2 DDGHVPVICCLARCNKADIDAAWEAVKHAKRPRIHTFIATSEIHLKYKLRKSRDEVIAIA 181
+DG+VPVIC L+RCN+ DI AWEAVK+AKRPRIHTFIATS IH++YKLRKS++EV+ IA
Sbjct: 154 EDGYVPVICGLSRCNEKDIRTAWEAVKYAKRPRIHTFIATSAIHMEYKLRKSKEEVVEIA 213
Query: 182 KEMVSYARSLGCNDVEFSPEDAGRSDREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFG 361
+ MV +ARSLGC+DVEFSPEDAGRSDREFLY IL EVIKAGATTLNIPDTVG TLP+EFG
Sbjct: 214 RSMVKFARSLGCDDVEFSPEDAGRSDREFLYHILGEVIKAGATTLNIPDTVGITLPSEFG 273
Query: 362 KLIADIKLNTPGIDDVIISTHCQNDLGLSTANTIERVGAGARQLEVTINGIGE 520
+LIADIK NTPGID VIISTHCQNDLGLSTANT+ AGARQ+EVTINGIGE
Sbjct: 274 QLIADIKANTPGIDKVIISTHCQNDLGLSTANTLAGAYAGARQVEVTINGIGE 326
Score = 58.2 bits (139), Expect(3) = 0.0
Identities = 31/53 (58%), Positives = 38/53 (71%), Gaps = 3/53 (5%)
Frame = +1
Query: 1246 ATHAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKMLDFSR---TSKVSGS 1395
+THA+TG+ RTFSG+GAGMDIVVSS YV ALNKML + T +VS +
Sbjct: 573 STHALTGEPFQRTFSGSGAGMDIVVSSVKAYVGALNKMLALKQQFLTKRVSST 625
>gb|AAW81741.1| Putative 2-isopropylmalate synthase [Brassica oleracea]
Length = 623
Score = 370 bits (951), Expect(3) = 0.0
Identities = 181/247 (73%), Positives = 215/247 (87%), Gaps = 1/247 (0%)
Frame = +3
Query: 516 ERAGNASLEEVVMAIRCRGEELLGGLHTGINTRHIVMASKMVAEFTGLQVQPHKAIVGAN 695
ERAGNASLEEVVMAI+CRG+ +LGGL+TGI+TRHIVM SKMV ++TG+Q QPHKAIVGAN
Sbjct: 319 ERAGNASLEEVVMAIKCRGDHVLGGLYTGIDTRHIVMTSKMVEDYTGMQTQPHKAIVGAN 378
Query: 696 AFAHESGIHQDGMLKYKGTYEIMSPEDIGLS*SNDSGIVLGKLSGRHALRSRLLELGYEL 875
AFAHESGIHQDGMLK+KGTYEI+ PE+IGL SND+GIVLGKLSGRHAL+ RL ELGY L
Sbjct: 379 AFAHESGIHQDGMLKHKGTYEIICPEEIGLERSNDAGIVLGKLSGRHALKDRLTELGYVL 438
Query: 876 MAE-LDVVFWRFKAVAEKKKHLSDGDIEALISDEIFQPQAIWSLGDLQVICGTLGLSTAT 1052
E L +FWRFK+VAE+KK ++D DI AL+SDE+FQP+A+W L D+Q+ CGTLGLSTAT
Sbjct: 439 DDEQLSSIFWRFKSVAERKKRVTDADIIALVSDEVFQPEALWKLLDIQITCGTLGLSTAT 498
Query: 1053 VKLIGIDGEEHICCSVGTGPVDAAYRAIDQIVKESVTLLEYSINSVTEGIDAIASTSVLI 1232
VKL DG+EH+ CS+GTGPVD+AY+A+D IVKE TLLEYS+N+VTEGIDAIA+T VLI
Sbjct: 499 VKLADADGKEHVACSMGTGPVDSAYKAVDLIVKEPATLLEYSMNAVTEGIDAIATTRVLI 558
Query: 1233 AGENGHS 1253
G N +S
Sbjct: 559 RGNNNYS 565
Score = 285 bits (728), Expect(3) = 0.0
Identities = 138/172 (80%), Positives = 157/172 (91%)
Frame = +2
Query: 5 DGHVPVICCLARCNKADIDAAWEAVKHAKRPRIHTFIATSEIHLKYKLRKSRDEVIAIAK 184
DG+VPVIC L+RCNK DI+ AWEAVK+AKRPRIHTFIATS+IHL+YKL+KS+DEVI A+
Sbjct: 148 DGYVPVICGLSRCNKRDIETAWEAVKYAKRPRIHTFIATSDIHLEYKLKKSKDEVIEFAR 207
Query: 185 EMVSYARSLGCNDVEFSPEDAGRSDREFLYKILEEVIKAGATTLNIPDTVGYTLPNEFGK 364
MV +ARSLGC DVEFSPEDAGRS+REFLY+IL EVIKAGATTLNIPDTVG TLP+EFG+
Sbjct: 208 NMVKFARSLGCEDVEFSPEDAGRSEREFLYQILGEVIKAGATTLNIPDTVGITLPSEFGQ 267
Query: 365 LIADIKLNTPGIDDVIISTHCQNDLGLSTANTIERVGAGARQLEVTINGIGE 520
LIADIK NTPGI++VIISTHCQNDLGLSTANT+ +GARQ+EVTINGIGE
Sbjct: 268 LIADIKANTPGIENVIISTHCQNDLGLSTANTLSGAHSGARQVEVTINGIGE 319
Score = 64.3 bits (155), Expect(3) = 0.0
Identities = 34/53 (64%), Positives = 40/53 (75%), Gaps = 2/53 (3%)
Frame = +1
Query: 1249 THAITGQTVHRTFSGAGAGMDIVVSSA*DYVSALNKMLDF--SRTSKVSGSSN 1401
T+AITG+ V RTFSG GAGMDIVVSS YV ALNKMLDF + T+K+ +N
Sbjct: 567 TNAITGEEVQRTFSGTGAGMDIVVSSVKAYVGALNKMLDFKENTTTKIPSQNN 619