BLASTX nr result
ID: Coptis24_contig00010477
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00010477
(2108 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|ADD71922.1| BAHD-type acyltransferase [Actaea racemosa] 326 1e-86
ref|XP_002314550.1| predicted protein [Populus trichocarpa] gi|2... 325 4e-86
ref|XP_002515007.1| 3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransf... 318 4e-84
ref|XP_002514982.1| 3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransf... 318 5e-84
ref|XP_002315639.1| predicted protein [Populus trichocarpa] gi|2... 315 4e-83
>gb|ADD71922.1| BAHD-type acyltransferase [Actaea racemosa]
Length = 424
Score = 326 bits (836), Expect = 1e-86
Identities = 189/435 (43%), Positives = 259/435 (59%), Gaps = 11/435 (2%)
Frame = -3
Query: 1404 LDLLVISRETIKPSSPTATHLRTYKLSNIDQYSPPFSVPIILFYSVGRDGD----TKVSD 1237
++++V SRE IKPSSPT HL+ S DQYS + V ++LFYS D D T SD
Sbjct: 1 MEVVVSSREIIKPSSPTPNHLKKLNFSLNDQYSTHY-VSVLLFYSAQGDVDRFKQTNTSD 59
Query: 1236 RLKDSFSETLTLFYPLGGKVVDNDTIDCDDSGVEYWETEIKSRLDEFLSNPVADEVDQLL 1057
RLK S SE LT FYPL G++++N+ IDC+D G+EY E + L + L P ADE++QL+
Sbjct: 60 RLKKSLSEILTQFYPLAGRIINNECIDCNDDGLEYLEARVPCPLSQLLGCPKADELNQLI 119
Query: 1056 PTYLHCRMSTETQLQIQVSTFECGGMAIGARISHKVADAATLCSFVNTWATKTSGTNKAV 877
P ++ +QVS F+CGG+AIG ISH DA++L +F+N+WA G N+ V
Sbjct: 120 P-------FSQKLSAVQVSLFDCGGIAIGVTISHTAGDASSLTAFINSWAATAKGANEIV 172
Query: 876 TPRFVSAALFPPRQVS--QFAPSDVGLPVQPRLVSKRFVFDSSDITLLKAKSAVTLHPTR 703
P+F LFPPR V F V PR V KRF+FDSS + LK+ A PTR
Sbjct: 173 PPKFGFDYLFPPRDVPTVSFGGGAVDYTQLPRFVGKRFIFDSSKLAALKSACADVERPTR 232
Query: 702 VQVVTALMWKCLMNASSKLRYRKSHVLNIAVNLRRRMVPALPENSFGNLII---GAMLSS 532
V+VVTA ++KC +N + + R K VL++ +NLR RM P LP +SFGNL I +
Sbjct: 233 VEVVTAFIYKCFLN-TIRSRSSKPSVLSMPINLRGRMNPPLPPHSFGNLAIRLTSQPWPA 291
Query: 531 ERGAELHSLVELLRKAVRKFDANYVKNLEKNDGVATFHH--SLIQSYIQSAEGELGLHTI 358
E+ EL+ LV+ LR+ +RK + +V+ L+ ++ F H + S G+L + +
Sbjct: 292 EKEPELNCLVKQLRETIRKVNGGFVEKLQADNAELLFEHWKDWKKGIELSLTGDLNVLMV 351
Query: 357 TSWCRFPFYDADFGWGKPIWVSSLNVAHQNVVILTDTKNGDGIEALVTLDEEDMAIFECE 178
CRFPFY+ADFGWGKP W + +N+ V L DTK+G+G+EA VTL E DM F E
Sbjct: 352 LICCRFPFYEADFGWGKPAWATRVNLYPS--VSLVDTKDGEGVEAWVTLAEGDMTRFCSE 409
Query: 177 EELLSFASPY*ANPP 133
+LL F+ NPP
Sbjct: 410 PDLLDFSI---ENPP 421
>ref|XP_002314550.1| predicted protein [Populus trichocarpa] gi|222863590|gb|EEF00721.1|
predicted protein [Populus trichocarpa]
Length = 441
Score = 325 bits (832), Expect = 4e-86
Identities = 189/435 (43%), Positives = 265/435 (60%), Gaps = 16/435 (3%)
Frame = -3
Query: 1392 VISRETIKPSSPTATHLRTYKLSNIDQYSPPFSVPIILFYSV-GRDGD--TKVSDR---L 1231
++ RETIKPSSPT HLR+ KLS +DQ+ P +P+ LFY G D D K ++R L
Sbjct: 9 IMQRETIKPSSPTPLHLRSLKLSLLDQFMPVGHIPLQLFYPRNGNDTDHLAKATERSLLL 68
Query: 1230 KDSFSETLTLFYPLGGKVVDNDTIDCDDSGVEYWETEIKSRLDEFLSNPVADEVDQLLPT 1051
K S SE LT FYP G++ DN +I+CDD G EY E I L + L P + + QL+P
Sbjct: 69 KTSLSEALTHFYPFAGRLKDNSSIECDDHGAEYIEARIHCILSDILKKPDTEVLKQLMPA 128
Query: 1050 YL-HCRMSTETQLQIQVSTFECGGMAIGARISHKVADAATLCSFVNTWA--TKTSGTNKA 880
+ + ++QL +Q S F+CGG+AIG +SHKVADAATL SF+ WA + S T
Sbjct: 129 AVSEPATARDSQLIVQASFFDCGGLAIGVNLSHKVADAATLTSFIKCWAATARRSSTEVV 188
Query: 879 VTPRFVSAALFPPRQVS-QFAPSDVGLPVQPRLVSKRFVFDSSDITLLKAK--SAVTLHP 709
++P F+ A++FP + P D+ +Q V KRFVF++ IT LKAK SA P
Sbjct: 189 ISPVFMGASIFPQMDLPISMLPVDL---IQGESVMKRFVFEAPKITALKAKAISASVPDP 245
Query: 708 TRVQVVTALMWKCLMNAS-SKLRYRKSHVLNIAVNLRRRMVPALPENSFGNLI--IGAML 538
TRV+ VTAL+WKC M+AS S L + VL++ VN+R+R+VP LP+N GN + I A +
Sbjct: 246 TRVESVTALIWKCAMSASRSNLGVPRKAVLSLGVNIRKRLVPTLPDNYGGNYVGSISARI 305
Query: 537 SSERGAELHSLVELLRKAVRKFDANYVKNLEKNDGVATFHHSLIQSYIQSAEGELGLHTI 358
EL +V +RK + +F NY K + +D ++ + + ++ +
Sbjct: 306 EDHDDLELQGIVSRIRKDLIEFGENYAKITQGDDTSLAICKAVEEFGKMAMSKDIDSYNG 365
Query: 357 TSWCRFPFYDADFGWGKPIWVSSL-NVAHQNVVILTDTKNGDGIEALVTLDEEDMAIFEC 181
TSWCRF YDADFGWGKP W+S++ + +N++ L DT++GDGIEA ++L EDMA+FE
Sbjct: 366 TSWCRFELYDADFGWGKPTWLSNVFTIELKNIMCLIDTRDGDGIEACISLSREDMALFES 425
Query: 180 EEELLSFASPY*ANP 136
+ELL FA+ ANP
Sbjct: 426 NKELLEFAA---ANP 437
>ref|XP_002515007.1| 3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase, putative [Ricinus
communis] gi|223546058|gb|EEF47561.1|
3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase,
putative [Ricinus communis]
Length = 441
Score = 318 bits (815), Expect = 4e-84
Identities = 184/434 (42%), Positives = 261/434 (60%), Gaps = 15/434 (3%)
Frame = -3
Query: 1410 MTLDLLVISRETIKPSSPTATHLRTYKLSNIDQYSPPFSVPIILFYSVGRDGD------- 1252
M + +I RETIKPSSPT L+ +KLS +DQ P +P++LFY DGD
Sbjct: 3 MAAKVKIIQRETIKPSSPTPPELKIHKLSLLDQLIPTNYIPVVLFYPAN-DGDNLDHHAN 61
Query: 1251 -TKVSDRLKDSFSETLTLFYPLGGKVVDNDTIDCDDSGVEYWETEIKSRLDEFLSNPVAD 1075
T+ S +LK S SETLT +YP G++ D+ +++CDD G ++ + I L + L +P A
Sbjct: 62 STERSLKLKTSLSETLTHYYPFAGRIKDSTSVECDDQGADFIQARINCLLSDVLKSPDAV 121
Query: 1074 EVDQLLPTYL-HCRMSTETQLQIQVSTFECGGMAIGARISHKVADAATLCSFVNTW-ATK 901
+ Q LP + +T L +Q + F CGG+A+G ISHK++DA TL +F+ W AT
Sbjct: 122 VLRQFLPAAITSTEAATGNLLLVQATFFHCGGLAVGVCISHKISDATTLKAFIKCWVATA 181
Query: 900 TSGTNKAVTPRFVSAALFPPRQVSQFAPSDVGLPVQPRLVSKRFVFDSSDITLLKAKSAV 721
TS + ++ TP F+ A++FPP +S P+ V ++ + ++KRFVF S I LKAK A
Sbjct: 182 TSSSTESATPLFMGASIFPPVDIS--IPTSVVELMKKQCITKRFVFTGSKIAALKAKVAS 239
Query: 720 TL--HPTRVQVVTALMWKCLMNAS-SKLRYRKSHVLNIAVNLRRRMVPALPENSFGNLII 550
T +PTRV+ V+ L+WK M A+ SKL Y + V ++ VN+R R +P LPE+ GN ++
Sbjct: 240 TTMRNPTRVETVSGLLWKTAMAATRSKLGYSRPSVWSMPVNMRTRFLPPLPESYAGNCLL 299
Query: 549 GAMLSSERGAELHSLVELLRKAVRKFDANYVKNLEKNDGV-ATFHHSLIQSYIQSA-EGE 376
+EL LV +RK + F NYVK L V ATF Q Y A +
Sbjct: 300 HINPKIADESELKELVGRIRKEIEGFRENYVKKLRGERAVLATF--GFFQEYGNLAMNND 357
Query: 375 LGLHTITSWCRFPFYDADFGWGKPIWVSSLNVAHQNVVILTDTKNGDGIEALVTLDEEDM 196
+ L+T TSWC+ YDADFGWG+P+WV ++ NVV L DT++GDGIEA +TL EE+M
Sbjct: 358 IDLYTCTSWCKLELYDADFGWGRPLWVGIDSIPLSNVVCLMDTRDGDGIEAWLTLGEENM 417
Query: 195 AIFECEEELLSFAS 154
A+FE +ELL FA+
Sbjct: 418 ALFESNQELLQFAA 431
>ref|XP_002514982.1| 3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase, putative [Ricinus
communis] gi|223546033|gb|EEF47536.1|
3'-N-debenzoyl-2'-deoxytaxol N-benzoyltransferase,
putative [Ricinus communis]
Length = 441
Score = 318 bits (814), Expect = 5e-84
Identities = 185/441 (41%), Positives = 268/441 (60%), Gaps = 11/441 (2%)
Frame = -3
Query: 1410 MTLDLLVISRETIKPSSPTATHLRTYKLSNIDQYSPPFSVPIILFYSVGRDGDT-KVSDR 1234
M +++ +I ++ IKPSSPT HLR +KLS +DQ SP ++L YS+ + S
Sbjct: 1 MEINVKIIKKDIIKPSSPTPNHLRIFKLSLLDQLSPAAYGSMLLVYSINKPSTAIDKSQL 60
Query: 1233 LKDSFSETLTLFYPLGGKVVDNDTIDCDDSGVEYWETEIKSRLDEFLSNPVADEVDQLLP 1054
LK S SETLT FYPL GK+ DN ++C+D G Y E ++ L + L P +V + L
Sbjct: 61 LKRSLSETLTRFYPLAGKIKDNSAVECNDDGAVYLEAQVDCLLSKLLEKPDDHQVIRNLI 120
Query: 1053 TYLHCRMSTETQ----LQIQVSTFECGGMAIGARISHKVADAATLCSFVNTWATKTSGTN 886
H + +ETQ L +Q + F CGG+AIG ISHK+ADA T+C+F+ WA GT+
Sbjct: 121 PAEHIQSLSETQAGCLLLVQATFFACGGLAIGVCISHKLADARTVCTFIQGWAAAALGTD 180
Query: 885 -KAVTPRFVSAALFPPRQVSQFAPSDVGLPV-QPRLVSKRFVFDSSDITLLKAKSAV--T 718
+AV P+F +++FPP+ + PS++ + + + + V+KR+VF +S I LK K+A
Sbjct: 181 HEAVRPKFNVSSIFPPQNL----PSELAVKLNEEKCVTKRYVFHASGIATLKDKAASESV 236
Query: 717 LHPTRVQVVTALMWKCLMNAS-SKLRYRKSHVLNIAVNLRRRMVPALPENSFGNLIIG-A 544
HPTRV+ VTAL+WKC M AS S + K VL +VN+R+++VP L E S GNL+ A
Sbjct: 237 RHPTRVEAVTALIWKCAMKASRSNSKQPKMSVLAQSVNIRKKLVPPLSEYSIGNLMGHFA 296
Query: 543 MLSSERGAELHSLVELLRKAVRKFDANYVKNLEKNDGVATFHHSLIQSYIQSAEGELGLH 364
+ ++E EL +LV +RK +++F NYVK L++ + + +L + G +
Sbjct: 297 LRATENEVELGTLVTKMRKGIQEFGENYVKKLQEGNALKAVFEALKEMGSLLHGGSTEFY 356
Query: 363 TITSWCRFPFYDADFGWGKPIWVSSLNVAHQNVVILTDTKNGDGIEALVTLDEEDMAIFE 184
+S CRFPFY DFGWGKP WV +N++ L D ++G+GIEA VTL EEDMAIFE
Sbjct: 357 ITSSLCRFPFYGIDFGWGKPAWVVPPRDPIKNIISLIDARDGNGIEAWVTLKEEDMAIFE 416
Query: 183 CEEELLSFASPY*ANPPSTMH 121
++ELL+ AS PS +H
Sbjct: 417 RDQELLAVAS----LNPSVLH 433
>ref|XP_002315639.1| predicted protein [Populus trichocarpa] gi|222864679|gb|EEF01810.1|
predicted protein [Populus trichocarpa]
Length = 430
Score = 315 bits (806), Expect = 4e-83
Identities = 186/437 (42%), Positives = 264/437 (60%), Gaps = 17/437 (3%)
Frame = -3
Query: 1410 MTLDLLVISRETIKPSSPTATHLRTYKLSNIDQYSPPFSVPIILFYSVGRDGDTKVSD-- 1237
M +++ VIS E IKPSSPT HL Y+LS +DQ SPP P++LFY DGD K+++
Sbjct: 1 MKIEIEVISNEIIKPSSPTPDHLCHYQLSFLDQISPPTYNPLLLFYPA--DGDVKINNIE 58
Query: 1236 ---RLKDSFSETLTLFYPLGGKVVDNDTIDCDDSGVEYWETEIKSRLDEFLSNPVADEVD 1066
+LK S SE L L+YPL G++ DN ++C+D G+ +++ E+K RL + + NP E++
Sbjct: 59 KPNQLKQSLSEVLNLYYPLAGRIKDNLFVECNDEGIPFFQAEVKCRLPQVVENPEPSELN 118
Query: 1065 QLLPTYLHCRMSTETQLQIQVSTFECGGMAIGARISHKVADAATLCSFVNTWATKTSGTN 886
+L+P L + E L IQ + FECGG+ IG ISHKV DA++L +F+ WA G
Sbjct: 119 KLIPFALDD--AEELPLGIQYNIFECGGIVIGLCISHKVGDASSLFTFIKYWAATARGEA 176
Query: 885 KAVT-PRFVSAALFPPRQVSQFAPSDVGLPVQPRLVSKRFVFDSSDITLLKAKSAVTLH- 712
++ P F+SA LFPP +S F P+ G+ + +V+KRFVF SS I LLK K +
Sbjct: 177 DHISRPEFISATLFPPINISGFKPA-TGITKED-VVTKRFVFRSSSIELLKEKCSPASGS 234
Query: 711 ------PTRVQVVTALMWKCLMNASSKLRYRKSHVLNI--AVNLRRRMVPALPENSFGNL 556
P+RV+ ++ +W+ A+SK+ R + ++ AVNLR RM P LPE SFGN
Sbjct: 235 LENQRPPSRVEALSVFIWQ-RFTAASKVESRPERIYSMVHAVNLRSRMEPPLPEYSFGNY 293
Query: 555 --IIGAMLSSERGAELHSLVELLRKAVRKFDANYVKNLEKNDGVATFHHSLIQSYIQSAE 382
I + S + G E ++L +R ++ K D YVK L+K + F +++
Sbjct: 294 CRIAFTIPSIDTGEENYNLASQIRDSIGKVDKEYVKKLQKGNEHLGFMKEQAARFLR--- 350
Query: 381 GELGLHTITSWCRFPFYDADFGWGKPIWVSSLNVAHQNVVILTDTKNGDGIEALVTLDEE 202
GE+ TS CRFP Y+ADFGW KPIWV S ++ +N+V+ DT +GDGIEALV L EE
Sbjct: 351 GEVVPLNFTSLCRFPLYEADFGWAKPIWVGSPSLTFKNLVVFMDTASGDGIEALVHLKEE 410
Query: 201 DMAIFECEEELLSFASP 151
DMA FE +EELL + P
Sbjct: 411 DMAKFEEDEELLQYIVP 427