BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00010446
         (1418 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002282833.1| PREDICTED: subtilisin-like protease [Vitis v...   467   e-129
gb|ACT34764.1| subtilisin-like protease preproenzyme [Nicotiana ...   460   e-127
ref|XP_002510195.1| Cucumisin precursor, putative [Ricinus commu...   448   e-123
ref|XP_002310134.1| predicted protein [Populus trichocarpa] gi|2...   447   e-123
ref|XP_002523606.1| Cucumisin precursor, putative [Ricinus commu...   445   e-122

>ref|XP_002282833.1| PREDICTED: subtilisin-like protease [Vitis vinifera]
          Length = 762

 Score =  467 bits (1202), Expect = e-129
 Identities = 239/439 (54%), Positives = 311/439 (70%)
 Frame = -2

Query: 1417 DGPSLYSVGNGAPWILTVGASSVDRTFSGTITLGNGVSIIGESLYPGNSSLSSVPLVYMK 1238
            +GP L ++ NG PW+LTV AS++DR FSG +TLGNGVS+IG SLYP NSS S +P+V+M 
Sbjct: 322  EGPFLGTLHNGIPWVLTVAASTMDRQFSGIVTLGNGVSVIGSSLYPANSSFSQIPIVFMG 381

Query: 1237 GCDDYDALEKVGYKIVVCVGIDTYNLPDKIFELNHHPVAGAILVGITSHAEDDIQTIFPA 1058
             C+D   L+KVG+KIVVC   +  +L  ++   N   VAG + +      E  +Q+ FPA
Sbjct: 382  SCEDLTELKKVGFKIVVCQDQND-SLSIQVDNANTARVAGGVFITDYPDIEFFMQSSFPA 440

Query: 1057 SLVNPKAGQHILHYINKSSDPKARLEFHKTNIGTRPAPIVGDYSSRGPSASCPDVLKPDL 878
            + VNP+ G+ ++ YI  SS+PKA +EF KT +G + AP +  YSSRGPS SCP VLKPDL
Sbjct: 441  TFVNPENGKVVMDYIKTSSEPKASIEFSKTILGAKRAPRMATYSSRGPSPSCPVVLKPDL 500

Query: 877  IAPGTLVLAAWPPNIRTTNPPSDVMLSPFNILSGTSMACPHAAGVAALLKGKHPEWSPAA 698
             APG L+LA+WP      +  S ++ S FN+LSGTSMACPHAAGV ALLKG HPEWSPAA
Sbjct: 501  TAPGALILASWPKINPVADVNSRLLYSEFNLLSGTSMACPHAAGVGALLKGAHPEWSPAA 560

Query: 697  IRSAMMTTAYTLDNTNKIIEQQGDFLRPSNALHMGAGHINPNKALNPGLIYDANAEDYMR 518
            IRSAMMTT+ +LDNT   I+  GD  +P++ L MG+GHINPNKAL+PG IYD N ED++ 
Sbjct: 561  IRSAMMTTSDSLDNTLNPIKGIGDDNQPASPLAMGSGHINPNKALDPGFIYDVNLEDHIN 620

Query: 517  FLCSMNYTKNQIKMITXXXXXXXXXXXSDINYPSFIAFFNSSTPSSSTKAVQEFRRTVTN 338
             LC++NY+  QI++IT            D+NYPSFIA F+++   S +K VQEFRRTVTN
Sbjct: 621  LLCALNYSTKQIQIITRSSSYTCSDPSLDLNYPSFIASFDANDSRSDSKTVQEFRRTVTN 680

Query: 337  VGDNMSTYTAKLTHMEEVKVSVVPNTLVFGKKYEKLTFILTIKGPARLYNQVVDGSLSWA 158
            VG+ MSTY AKLT M+  +VSVVP+ LVF  KY+KL++ L I+GP+ +   V  GSLSW 
Sbjct: 681  VGEAMSTYNAKLTGMDGFQVSVVPDKLVFKDKYQKLSYKLRIEGPSLMKETVAFGSLSWV 740

Query: 157  HDKGKYIVRSPIVVTRANL 101
              + K++VRSPIV TR +L
Sbjct: 741  DVEAKHVVRSPIVATRLSL 759


>gb|ACT34764.1| subtilisin-like protease preproenzyme [Nicotiana tabacum]
          Length = 763

 Score =  460 bits (1183), Expect = e-127
 Identities = 232/440 (52%), Positives = 301/440 (68%)
 Frame = -2

Query: 1417 DGPSLYSVGNGAPWILTVGASSVDRTFSGTITLGNGVSIIGESLYPGNSSLSSVPLVYMK 1238
            +GP   ++ NG PW+LTV A +VDR F GT+TLGNGVS+ G SLYPGNSS S   +V++K
Sbjct: 323  EGPDGQTLHNGTPWVLTVAAGTVDREFIGTLTLGNGVSVTGLSLYPGNSSSSESSIVFLK 382

Query: 1237 GCDDYDALEKVGYKIVVCVGIDTYNLPDKIFELNHHPVAGAILVGITSHAEDDIQTIFPA 1058
             C +   LEK   KI +C   +  ++ D+++ + +  VAG + +   +  E  +Q+ FPA
Sbjct: 383  TCLEEKELEKNANKIAICYDTNG-SISDQLYNVRNSKVAGGVFITNYTDLEFYLQSEFPA 441

Query: 1057 SLVNPKAGQHILHYINKSSDPKARLEFHKTNIGTRPAPIVGDYSSRGPSASCPDVLKPDL 878
              +N + G  +L YI  S  PKARLEF  T++GT+PAP V  YSSRGPS SCP +LKPDL
Sbjct: 442  VFLNFEDGDKVLEYIKNSHSPKARLEFQVTHLGTKPAPKVASYSSRGPSQSCPFILKPDL 501

Query: 877  IAPGTLVLAAWPPNIRTTNPPSDVMLSPFNILSGTSMACPHAAGVAALLKGKHPEWSPAA 698
            +APG L+LA+WP     T   S  + S FNI+SGTSM+CPHAAGVA+LLKG HP+WSPAA
Sbjct: 502  MAPGALILASWPQKSPATKINSGELFSNFNIISGTSMSCPHAAGVASLLKGAHPKWSPAA 561

Query: 697  IRSAMMTTAYTLDNTNKIIEQQGDFLRPSNALHMGAGHINPNKALNPGLIYDANAEDYMR 518
            IRSAMMTTA  LDNT + I   G     ++ L MGAGHINPNKAL+PGLIYD  ++DY+ 
Sbjct: 562  IRSAMMTTADALDNTQRPIRDIGRNNNAASPLAMGAGHINPNKALDPGLIYDITSQDYIN 621

Query: 517  FLCSMNYTKNQIKMITXXXXXXXXXXXSDINYPSFIAFFNSSTPSSSTKAVQEFRRTVTN 338
             LC++++T  QIK IT            D+NYPSFI +FN ++  S  K +QEF+RTVTN
Sbjct: 622  LLCALDFTSQQIKAITRSSAYSCSNPSLDLNYPSFIGYFNYNSSKSDPKRIQEFQRTVTN 681

Query: 337  VGDNMSTYTAKLTHMEEVKVSVVPNTLVFGKKYEKLTFILTIKGPARLYNQVVDGSLSWA 158
            VGD MS YTAKLT M+E KVSV P+ LVF +KYEK ++ L I+GP  + N +V GSLSW 
Sbjct: 682  VGDGMSVYTAKLTSMDEYKVSVAPDKLVFKEKYEKQSYKLRIEGPLLVDNYLVYGSLSWV 741

Query: 157  HDKGKYIVRSPIVVTRANLE 98
               GKY+V+SPIV T   ++
Sbjct: 742  ETSGKYVVKSPIVATTIGVD 761


>ref|XP_002510195.1| Cucumisin precursor, putative [Ricinus communis]
            gi|223550896|gb|EEF52382.1| Cucumisin precursor, putative
            [Ricinus communis]
          Length = 768

 Score =  448 bits (1152), Expect = e-123
 Identities = 231/436 (52%), Positives = 299/436 (68%)
 Frame = -2

Query: 1417 DGPSLYSVGNGAPWILTVGASSVDRTFSGTITLGNGVSIIGESLYPGNSSLSSVPLVYMK 1238
            +GP L ++ NG PW+LTV A +VDR FS T+TL NG S+ G +LYPGN S S VP+V+  
Sbjct: 328  EGPYLETLHNGIPWVLTVAAGTVDREFSATVTLENGASVTGSALYPGNYSSSQVPIVFFD 387

Query: 1237 GCDDYDALEKVGYKIVVCVGIDTYNLPDKIFELNHHPVAGAILVGITSHAEDDIQTIFPA 1058
             C D   L KVG KIVVC   +  +L D+   L    ++G I +   +  E  IQ+ FPA
Sbjct: 388  SCLDSKELNKVGKKIVVCEDKNA-SLDDQFDNLRKVNISGGIFITNFTDLELFIQSGFPA 446

Query: 1057 SLVNPKAGQHILHYINKSSDPKARLEFHKTNIGTRPAPIVGDYSSRGPSASCPDVLKPDL 878
              V+PK G+ I  +IN S+ P+A +EF KTN G + AP +  YSSRGPS SCP V+KPD+
Sbjct: 447  IFVSPKDGETIKDFINSSTSPQASMEFQKTNFGIKSAPSLASYSSRGPSPSCPYVMKPDI 506

Query: 877  IAPGTLVLAAWPPNIRTTNPPSDVMLSPFNILSGTSMACPHAAGVAALLKGKHPEWSPAA 698
            + PG+L+LAAWP NI      S  + S FNILSGTSM+CPHAAGVAALLK  HP+WSPAA
Sbjct: 507  MGPGSLILAAWPQNIEVMRLNSKPLFSNFNILSGTSMSCPHAAGVAALLKNAHPDWSPAA 566

Query: 697  IRSAMMTTAYTLDNTNKIIEQQGDFLRPSNALHMGAGHINPNKALNPGLIYDANAEDYMR 518
            IRSAMMT+  T+D+T   I+  G+  +P++ L MGAG +NP+KAL+PGLIYD  + DY++
Sbjct: 567  IRSAMMTSVVTMDHTPGPIKDIGNNNQPASPLDMGAGQVNPSKALDPGLIYDLKSTDYVK 626

Query: 517  FLCSMNYTKNQIKMITXXXXXXXXXXXSDINYPSFIAFFNSSTPSSSTKAVQEFRRTVTN 338
             LC++N+T+ QI++IT            D+NYPSFIAFFNS+   SST  VQEF RTVTN
Sbjct: 627  LLCALNFTEKQIQIITRSSSNDCSSPSLDLNYPSFIAFFNSNVSKSST--VQEFHRTVTN 684

Query: 337  VGDNMSTYTAKLTHMEEVKVSVVPNTLVFGKKYEKLTFILTIKGPARLYNQVVDGSLSWA 158
            VG+ MSTYTA LT +  +KVSVVP+ L F  K EKL++ L I+GP  L   ++ G LSW 
Sbjct: 685  VGEGMSTYTANLTPINGLKVSVVPDKLEFKAKNEKLSYKLVIEGPTMLKESIIFGYLSWV 744

Query: 157  HDKGKYIVRSPIVVTR 110
             D+GK+ V+SPIV TR
Sbjct: 745  DDEGKHTVKSPIVATR 760


>ref|XP_002310134.1| predicted protein [Populus trichocarpa] gi|222853037|gb|EEE90584.1|
            predicted protein [Populus trichocarpa]
          Length = 725

 Score =  447 bits (1151), Expect = e-123
 Identities = 228/435 (52%), Positives = 297/435 (68%), Gaps = 1/435 (0%)
 Frame = -2

Query: 1414 GPSLYSVGNGAPWILTVGASSVDRTFSGTITLGNGVSIIGESLYPGNSSLSSVPLVYMKG 1235
            GP+ Y++ NGAPW+LT+GA ++DR F G +TLGNG  I   ++YPGN SLS  PLV+M G
Sbjct: 289  GPNYYTLVNGAPWMLTIGAGTIDREFEGVLTLGNGNQISFPTVYPGNYSLSHKPLVFMDG 348

Query: 1234 CDDYDALEKVGYKIVVCVGIDTYNLPDKIFELNHHPVAGAILVGI-TSHAEDDIQTIFPA 1058
            C+  + L+KV  KI+VC   D     D+I       V+GA+ +   TS +E   ++ FPA
Sbjct: 349  CESVNELKKVKNKIIVCK--DNLTFSDQIDNAASARVSGAVFISNHTSPSEFYTRSSFPA 406

Query: 1057 SLVNPKAGQHILHYINKSSDPKARLEFHKTNIGTRPAPIVGDYSSRGPSASCPDVLKPDL 878
              +  + GQ ++ YI +S DP+  + F KT  GT+PAP V  YS RGP ASC  VLKPDL
Sbjct: 407  VYIGLQDGQRVIDYIKESKDPRGTVVFRKTVTGTKPAPRVDGYSGRGPFASCRSVLKPDL 466

Query: 877  IAPGTLVLAAWPPNIRTTNPPSDVMLSPFNILSGTSMACPHAAGVAALLKGKHPEWSPAA 698
            +APGTLVLA+W P        S  + S FN+LSGTSMA PH AGVAAL+K  HP+WSPAA
Sbjct: 467  LAPGTLVLASWSPISSVAEVRSHSLFSKFNLLSGTSMATPHVAGVAALIKKAHPDWSPAA 526

Query: 697  IRSAMMTTAYTLDNTNKIIEQQGDFLRPSNALHMGAGHINPNKALNPGLIYDANAEDYMR 518
            IRSA+MTTA +LDNT   I+   +   P+  + +G+GHINPNK+L+PGLIYDA AEDY++
Sbjct: 527  IRSALMTTADSLDNTLSPIKDASNNNLPATPIDIGSGHINPNKSLDPGLIYDATAEDYIK 586

Query: 517  FLCSMNYTKNQIKMITXXXXXXXXXXXSDINYPSFIAFFNSSTPSSSTKAVQEFRRTVTN 338
             LC+MNYT  QI++IT            D+NYPSFIA+F+S    S  K V +F+RT+TN
Sbjct: 587  LLCAMNYTNKQIQIITRSSHHDCKNRSLDLNYPSFIAYFDSYDSGSKEKVVHKFQRTLTN 646

Query: 337  VGDNMSTYTAKLTHMEEVKVSVVPNTLVFGKKYEKLTFILTIKGPARLYNQVVDGSLSWA 158
            VG+ MS+YTAKL  M+ +KVSV P  LVF K++EKL++ LT++GP  L   V+ GSLSW 
Sbjct: 647  VGERMSSYTAKLLGMDGIKVSVEPQKLVFKKEHEKLSYTLTLEGPKSLEEDVIHGSLSWV 706

Query: 157  HDKGKYIVRSPIVVT 113
            HD GKY+VRSPIV T
Sbjct: 707  HDGGKYVVRSPIVAT 721


>ref|XP_002523606.1| Cucumisin precursor, putative [Ricinus communis]
            gi|223537168|gb|EEF38801.1| Cucumisin precursor, putative
            [Ricinus communis]
          Length = 768

 Score =  445 bits (1144), Expect = e-122
 Identities = 223/435 (51%), Positives = 302/435 (69%)
 Frame = -2

Query: 1417 DGPSLYSVGNGAPWILTVGASSVDRTFSGTITLGNGVSIIGESLYPGNSSLSSVPLVYMK 1238
            DGP+ +++ NGAPW+LTVGA ++DR F G +TLG+G  I   +LYPG SSLS +PLV++ 
Sbjct: 330  DGPAYWTLVNGAPWLLTVGAGTIDREFKGILTLGDGKRISFNTLYPGKSSLSEIPLVFLN 389

Query: 1237 GCDDYDALEKVGYKIVVCVGIDTYNLPDKIFELNHHPVAGAILVGITSHAEDDIQTIFPA 1058
            GC++   +EK   +IVVC   D  ++ D++       V+GAI +   + +E   ++ +PA
Sbjct: 390  GCENMQEMEKYKNRIVVCK--DNLSISDQVQNAAKARVSGAIFITDITLSEYYTRSSYPA 447

Query: 1057 SLVNPKAGQHILHYINKSSDPKARLEFHKTNIGTRPAPIVGDYSSRGPSASCPDVLKPDL 878
            + +  K GQ ++ YI  S++P   L+F KT +GT+PAP V  YSSRGP  SC  VLKPD+
Sbjct: 448  AFIGLKDGQSVVEYIRSSNNPIGNLQFQKTVLGTKPAPKVDSYSSRGPFTSCQYVLKPDI 507

Query: 877  IAPGTLVLAAWPPNIRTTNPPSDVMLSPFNILSGTSMACPHAAGVAALLKGKHPEWSPAA 698
            +APG+LVLA+W P    T   S  + S FN+LSGTSMA PH AG+AAL+K  HP+WSPAA
Sbjct: 508  LAPGSLVLASWSPMSSVTEVRSHPIFSKFNLLSGTSMATPHVAGIAALIKKAHPDWSPAA 567

Query: 697  IRSAMMTTAYTLDNTNKIIEQQGDFLRPSNALHMGAGHINPNKALNPGLIYDANAEDYMR 518
            IRSA+MTT+ +LDNT   I+   +   P+N L +GAGH++PNK+L+PGLIYDA A+DYM+
Sbjct: 568  IRSALMTTSNSLDNTRTPIKDASNHDLPANPLDIGAGHVDPNKSLDPGLIYDATADDYMK 627

Query: 517  FLCSMNYTKNQIKMITXXXXXXXXXXXSDINYPSFIAFFNSSTPSSSTKAVQEFRRTVTN 338
             LC+MNYTK QI++IT            D+NYPSFIA+FN+     + K V+EFRRT+TN
Sbjct: 628  LLCAMNYTKKQIQIIT-RSNPNCVNKSLDLNYPSFIAYFNNDDSDLNEKVVREFRRTLTN 686

Query: 337  VGDNMSTYTAKLTHMEEVKVSVVPNTLVFGKKYEKLTFILTIKGPARLYNQVVDGSLSWA 158
            VG  MS+Y+AK+T M  V+ +V P  LVF  KYEKL++ LT++GP  L   VV GSLSW 
Sbjct: 687  VGMGMSSYSAKVTPMYGVRATVEPKELVFRNKYEKLSYKLTLEGPKILEEMVVHGSLSWV 746

Query: 157  HDKGKYIVRSPIVVT 113
            HD+GKY+V SPIV T
Sbjct: 747  HDEGKYVVTSPIVAT 761