BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00010217
         (1518 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283889.2| PREDICTED: aspartic proteinase nepenthesin-1...   589   e-166
ref|XP_002514026.1| Aspartic proteinase nepenthesin-1 precursor,...   581   e-163
ref|XP_002302336.1| predicted protein [Populus trichocarpa] gi|2...   568   e-159
ref|XP_004148418.1| PREDICTED: aspartic proteinase nepenthesin-1...   567   e-159
gb|ABK95290.1| unknown [Populus trichocarpa]                          563   e-158

>ref|XP_002283889.2| PREDICTED: aspartic proteinase nepenthesin-1-like [Vitis vinifera]
          Length = 439

 Score =  589 bits (1519), Expect = e-166
 Identities = 287/441 (65%), Positives = 342/441 (77%), Gaps = 9/441 (2%)
 Frame = -2

Query: 1463 MALAIAFLFFSILISTTTTYGLDSCTS-------SDLSIVHIYGKCSPFTSPKRESWVDT 1305
            M +  AF+F ++++STT  +  D C S       SDLS++H+YG+CSPF   K  SWV+T
Sbjct: 1    MDIFTAFVFLTLVVSTTKAF--DPCASPSSESKGSDLSVIHVYGQCSPFNQHKAGSWVNT 58

Query: 1304 VIDMAKKDPLRVTYLSMLIG--EKTSVPIASGQQVLNIGNYVVRAKFGTPGQLVYMVMDT 1131
            VI+MA KDP RVTYLS L+   + TSVPIASGQQVLNIGNYVVR K GTPGQL++MV+DT
Sbjct: 59   VINMASKDPARVTYLSSLVASPKATSVPIASGQQVLNIGNYVVRVKLGTPGQLMFMVLDT 118

Query: 1130 SNDAAWIPCSGCSGCSPTMFSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSY 951
            S DAAW+PC+ C+GCS   FSPN S+TY  + C +PQCT VRGLSCP +G + C FNQ+Y
Sbjct: 119  SRDAAWVPCADCAGCSSPTFSPNTSSTYASLQCSVPQCTQVRGLSCPTTGTAACFFNQTY 178

Query: 950  GGDSSFSAILSQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXGAL 771
            GGDSSFSA+LSQDSL L +D +P Y FGC+++VSG  +PPQ                G+L
Sbjct: 179  GGDSSFSAMLSQDSLGLAVDTLPSYSFGCVNAVSGSTLPPQGLLGLGRGPMSLLSQSGSL 238

Query: 770  YSGVFSYCLPSFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVL 591
            YSGVFSYC PSFKSYYFSGSL+LGP GQPK I+TTPLLRNPHRP+LYYVNLTG+SVG+VL
Sbjct: 239  YSGVFSYCFPSFKSYYFSGSLRLGPLGQPKNIRTTPLLRNPHRPTLYYVNLTGVSVGRVL 298

Query: 590  VPVAPELLAFDPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFT 411
            VPVAPELLAFDP+TGAGTIIDSGTVITRFV PVY A+RDEFRK + G  +++GAFDTCF 
Sbjct: 299  VPVAPELLAFDPNTGAGTIIDSGTVITRFVEPVYAAIRDEFRKQVKGPFATIGAFDTCFA 358

Query: 410  SSNEALTPLITFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXSNVNSVLNVIANLQQQ 231
            ++NE + P +TFHF GMDLKLP+ENTLIH               +NVNSVLNVIANLQQQ
Sbjct: 359  ATNEDIAPPVTFHFTGMDLKLPLENTLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQ 418

Query: 230  NLRILFDLTNSKLGIGRELCN 168
            NLRI+FD+TNS+LGI RELCN
Sbjct: 419  NLRIMFDVTNSRLGIARELCN 439


>ref|XP_002514026.1| Aspartic proteinase nepenthesin-1 precursor, putative [Ricinus
            communis] gi|223547112|gb|EEF48609.1| Aspartic proteinase
            nepenthesin-1 precursor, putative [Ricinus communis]
          Length = 437

 Score =  581 bits (1497), Expect = e-163
 Identities = 285/431 (66%), Positives = 338/431 (78%), Gaps = 5/431 (1%)
 Frame = -2

Query: 1445 FLFFSILISTTTTYGLDSCTS----SDLSIVHIYGKCSPFTSPKRESWVDTVIDMAKKDP 1278
            FL F++L+S+T    LD C S    SDLSI+ IY KCSPF  PK+E  V+TVIDMA KDP
Sbjct: 9    FLLFALLVSSTIA--LDPCASQADDSDLSIIPIYSKCSPFIPPKQEPLVNTVIDMASKDP 66

Query: 1277 LRVTYLSMLIGEKTS-VPIASGQQVLNIGNYVVRAKFGTPGQLVYMVMDTSNDAAWIPCS 1101
             R+ YLS L  + T+ VPIA GQQVLNIGNYVVR K GTPGQ ++MV+DTSNDAAW+PCS
Sbjct: 67   ARLKYLSSLAAQMTTAVPIAPGQQVLNIGNYVVRVKLGTPGQFMFMVLDTSNDAAWVPCS 126

Query: 1100 GCSGCSPTMFSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSYGGDSSFSAIL 921
            GC+GCS T FS N S+TYG +DC M QCT VRG SCP +G S C+FNQSYGGDSSFSA L
Sbjct: 127  GCTGCSSTTFSTNTSSTYGSLDCSMAQCTQVRGFSCPATGSSSCVFNQSYGGDSSFSATL 186

Query: 920  SQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXGALYSGVFSYCLP 741
             +DSLRL  D++P++ FGC++S+SGG++PPQ                G+LYSG+FSYCLP
Sbjct: 187  VEDSLRLVNDVIPNFAFGCINSISGGSVPPQGLLGLGRGPLSLIAQSGSLYSGLFSYCLP 246

Query: 740  SFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVLVPVAPELLAF 561
            SFKSYYFSGSLKLGP GQPK+I+ TPLLRNPHRPSLYYVNLTG+SVG+ LVP+APELLAF
Sbjct: 247  SFKSYYFSGSLKLGPAGQPKSIRYTPLLRNPHRPSLYYVNLTGVSVGRTLVPIAPELLAF 306

Query: 560  DPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFTSSNEALTPLI 381
            +P+TGAGTIIDSGTVITRFV P+Y A+RDEFRK ++G  SSLGAFDTCF ++NEA+ P +
Sbjct: 307  NPNTGAGTIIDSGTVITRFVQPIYTAIRDEFRKQVAGPFSSLGAFDTCFAATNEAVAPAV 366

Query: 380  TFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXSNVNSVLNVIANLQQQNLRILFDLTN 201
            T HF G++L LPMEN+LIH               +NVNSVLNVIANLQQQNLR+LFD+ N
Sbjct: 367  TLHFTGLNLVLPMENSLIHSSAGSLACLAMAAAPNNVNSVLNVIANLQQQNLRLLFDVPN 426

Query: 200  SKLGIGRELCN 168
            S+LGI RELCN
Sbjct: 427  SRLGIARELCN 437


>ref|XP_002302336.1| predicted protein [Populus trichocarpa] gi|222844062|gb|EEE81609.1|
            predicted protein [Populus trichocarpa]
          Length = 438

 Score =  568 bits (1463), Expect = e-159
 Identities = 281/434 (64%), Positives = 330/434 (76%), Gaps = 5/434 (1%)
 Frame = -2

Query: 1454 AIAFLFFSILISTTTTYGLDSCTS----SDLSIVHIYGKCSPFTSPKRESWVDTVIDMAK 1287
            A  F  F++L STT    +D C +    SDLS++ IY KCSPF  PK+ESWV+TVI MA 
Sbjct: 7    AATFFLFALLFSTTKA--VDPCATQSDTSDLSVIPIYSKCSPFVPPKQESWVNTVITMAS 64

Query: 1286 KDPLRVTYLSMLIGEKTS-VPIASGQQVLNIGNYVVRAKFGTPGQLVYMVMDTSNDAAWI 1110
            KDP R+ YLS L  +KT+ VPIA GQQVL I NYVVR K GTPGQ ++MV+DTSNDAAW+
Sbjct: 65   KDPERLKYLSTLADQKTTAVPIAPGQQVLKIANYVVRVKLGTPGQQMFMVLDTSNDAAWV 124

Query: 1109 PCSGCSGCSPTMFSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSYGGDSSFS 930
            PCSGC+GCS T F PN STT G +DC   QC+ VRG SCP +G S CLFNQSYGGDSS +
Sbjct: 125  PCSGCTGCSSTTFLPNASTTLGSLDCSGAQCSQVRGFSCPATGSSACLFNQSYGGDSSLT 184

Query: 929  AILSQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXGALYSGVFSY 750
            A L QD++ L  D++P + FGC+++VSGG+IPPQ                GA+YSGVFSY
Sbjct: 185  ATLVQDAITLANDVIPGFTFGCINAVSGGSIPPQGLLGLGRGPISLISQAGAMYSGVFSY 244

Query: 749  CLPSFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVLVPVAPEL 570
            CLPSFKSYYFSGSLKLGP GQPK+I+TTPLLRNPHRPSLYYVNLTG+SVG++ VP+  E 
Sbjct: 245  CLPSFKSYYFSGSLKLGPVGQPKSIRTTPLLRNPHRPSLYYVNLTGVSVGRIKVPIPSEQ 304

Query: 569  LAFDPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFTSSNEALT 390
            L FDP+TGAGTIIDSGTVITRFV PVY A+RDEFRK ++G ISSLGAFDTCF ++NEA  
Sbjct: 305  LVFDPNTGAGTIIDSGTVITRFVQPVYFAIRDEFRKQVNGPISSLGAFDTCFAATNEAEA 364

Query: 389  PLITFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXSNVNSVLNVIANLQQQNLRILFD 210
            P IT HF+G++L LPMEN+LIH               +NVNSVLNVIANLQQQNLRI+FD
Sbjct: 365  PAITLHFEGLNLVLPMENSLIHSSSGSLACLSMAAAPNNVNSVLNVIANLQQQNLRIMFD 424

Query: 209  LTNSKLGIGRELCN 168
             TNS+LGI RELCN
Sbjct: 425  TTNSRLGIARELCN 438


>ref|XP_004148418.1| PREDICTED: aspartic proteinase nepenthesin-1-like [Cucumis sativus]
            gi|449518059|ref|XP_004166061.1| PREDICTED: aspartic
            proteinase nepenthesin-1-like [Cucumis sativus]
          Length = 436

 Score =  567 bits (1462), Expect = e-159
 Identities = 282/437 (64%), Positives = 330/437 (75%), Gaps = 5/437 (1%)
 Frame = -2

Query: 1463 MALAIAFLFFSILISTTTTYGLDSCTSS---DLSIVHIYGKCSPFTSPKRESWVDTVIDM 1293
            MAL + F F S+ +   T+   D C S    DLS++ IYGKCSPFT+PK ESW++TVIDM
Sbjct: 1    MALKL-FFFLSLALLLLTSNAFDLCASGSDGDLSVIPIYGKCSPFTAPKSESWMNTVIDM 59

Query: 1292 AKKDPLRVTYLSMLIGEKT-SVPIASGQQVLNIGNYVVRAKFGTPGQLVYMVMDTSNDAA 1116
            A KDP R+ YLS L  +KT + PIASGQQVLN+GNYVVR + GTPGQ +YMV+DTSNDAA
Sbjct: 60   ASKDPARIRYLSSLTAQKTVAAPIASGQQVLNVGNYVVRVQLGTPGQTMYMVLDTSNDAA 119

Query: 1115 WIPCSGCSGCSPTM-FSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSYGGDS 939
            W PCSGC GCS T  FS   S+T+  +DC  P+CT  RGLSCP +G   CLFNQ+YGGDS
Sbjct: 120  WAPCSGCIGCSSTTTFSAQNSSTFATLDCSKPECTQARGLSCPTTGNVDCLFNQTYGGDS 179

Query: 938  SFSAILSQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXGALYSGV 759
            +FSA L QDSL LG +++P++ FGC+SS SG +IPPQ                G+LYSG+
Sbjct: 180  TFSATLVQDSLHLGPNVIPNFSFGCISSASGSSIPPQGLMGLGRGPLSLISQSGSLYSGL 239

Query: 758  FSYCLPSFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVLVPVA 579
            FSYCLPSFKSYYFSGSLKLGP GQPK I+TTPLL NPHRPSLYYVNLTGISVG+VLVP++
Sbjct: 240  FSYCLPSFKSYYFSGSLKLGPVGQPKAIRTTPLLHNPHRPSLYYVNLTGISVGRVLVPIS 299

Query: 578  PELLAFDPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFTSSNE 399
            PELLAFDP+TGAGTIIDSGTVITRFV  +Y A+RDEFRK + G+ S LGAFDTCF ++NE
Sbjct: 300  PELLAFDPNTGAGTIIDSGTVITRFVPAIYTAVRDEFRKQVGGSFSPLGAFDTCFATNNE 359

Query: 398  ALTPLITFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXSNVNSVLNVIANLQQQNLRI 219
               P IT H  G+DLKLPMEN+LIH               +NVNSV+NVIANLQQQN RI
Sbjct: 360  VSAPAITLHLSGLDLKLPMENSLIHSSAGSLACLAMAAAPNNVNSVVNVIANLQQQNHRI 419

Query: 218  LFDLTNSKLGIGRELCN 168
            LFD+ NSKLGI RELCN
Sbjct: 420  LFDINNSKLGIARELCN 436


>gb|ABK95290.1| unknown [Populus trichocarpa]
          Length = 438

 Score =  563 bits (1450), Expect = e-158
 Identities = 278/432 (64%), Positives = 328/432 (75%), Gaps = 5/432 (1%)
 Frame = -2

Query: 1448 AFLFFSILISTTTTYGLDSCTS----SDLSIVHIYGKCSPFTSPKRESWVDTVIDMAKKD 1281
            A  FF + +  +TT  +D C +    SDLS++ IY KCSPF  PK+ESWV+TVI MA KD
Sbjct: 7    AATFFLVALLFSTTKAVDPCATQSDTSDLSVIPIYSKCSPFVPPKQESWVNTVITMASKD 66

Query: 1280 PLRVTYLSMLIGEKTS-VPIASGQQVLNIGNYVVRAKFGTPGQLVYMVMDTSNDAAWIPC 1104
            P R+ YLS L  +KT+ VPIA GQQVL I NYVVR K GTPGQ ++MV+DTSNDAAW+PC
Sbjct: 67   PERLKYLSTLADQKTTAVPIAPGQQVLKIANYVVRVKLGTPGQQMFMVLDTSNDAAWVPC 126

Query: 1103 SGCSGCSPTMFSPNVSTTYGFMDCKMPQCTLVRGLSCPDSGPSPCLFNQSYGGDSSFSAI 924
            SGC+G S T F PN STT G +DC   QC+ VRG SCP +G S CLFNQSYGGDSS +A 
Sbjct: 127  SGCTGFSSTTFLPNASTTLGSLDCSGAQCSQVRGFSCPATGSSACLFNQSYGGDSSLTAT 186

Query: 923  LSQDSLRLGLDIVPDYVFGCLSSVSGGNIPPQXXXXXXXXXXXXXXXXGALYSGVFSYCL 744
            L QD++ L  D++P + FGC+++VSGG+IPPQ                GA+YSGVFSYCL
Sbjct: 187  LVQDAITLANDVIPGFTFGCINAVSGGSIPPQGLLGLGRGPISLISQAGAMYSGVFSYCL 246

Query: 743  PSFKSYYFSGSLKLGPTGQPKTIKTTPLLRNPHRPSLYYVNLTGISVGKVLVPVAPELLA 564
            PSFKSYYFSGSLKLGP GQPK+I+TTPLLRNPHRPSLYYVNLTG+SVG++ VP+  E L 
Sbjct: 247  PSFKSYYFSGSLKLGPVGQPKSIRTTPLLRNPHRPSLYYVNLTGVSVGRIKVPIPSEQLV 306

Query: 563  FDPSTGAGTIIDSGTVITRFVAPVYIAMRDEFRKHISGTISSLGAFDTCFTSSNEALTPL 384
            FDP+TGAGTIIDSGTVITRFV PVY A+RDEFRK ++G ISSLGAFDTCF ++NEA  P 
Sbjct: 307  FDPNTGAGTIIDSGTVITRFVQPVYFAIRDEFRKQVNGPISSLGAFDTCFAATNEAEAPA 366

Query: 383  ITFHFDGMDLKLPMENTLIHXXXXXXXXXXXXXXXSNVNSVLNVIANLQQQNLRILFDLT 204
            IT HF+G++L LPMEN+LIH               +NVNSVLNVIANLQQQNLRI+FD T
Sbjct: 367  ITLHFEGLNLVLPMENSLIHSSSGSLACLSMAAAPNNVNSVLNVIANLQQQNLRIMFDTT 426

Query: 203  NSKLGIGRELCN 168
            NS+LGI RELCN
Sbjct: 427  NSRLGIARELCN 438