BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00010185
         (1326 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABC61505.1| AGO4-2, partial [Nicotiana benthamiana]                360   3e-97
gb|ACZ04920.1| argonaute 4-like protein [Pelargonium x hortorum]      357   5e-96
gb|ABC61504.1| AGO4-1, partial [Nicotiana benthamiana]                356   9e-96
gb|AFV15381.1| AGO4A [Solanum lycopersicum]                           354   3e-95
gb|AFV15382.1| AGO4B [Solanum lycopersicum]                           352   1e-94

>gb|ABC61505.1| AGO4-2, partial [Nicotiana benthamiana]
          Length = 905

 Score =  360 bits (925), Expect = 3e-97
 Identities = 184/216 (85%), Positives = 196/216 (90%), Gaps = 2/216 (0%)
 Frame = -1

Query: 1326 FLLCLLPERKTSAIYGPWKRKNLADFGIVTQCIAPTKVNDQYLTNVLLKINAKLGGLNA- 1150
            FLLCLLPERK   IYGPWKRKNLA+FGIVTQCIAPT+VNDQY+TNVLLKINAKLGGLN+ 
Sbjct: 558  FLLCLLPERKNCDIYGPWKRKNLAEFGIVTQCIAPTRVNDQYITNVLLKINAKLGGLNSM 617

Query: 1149 -TVECSPSIPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSRRWPSISRYRASVRTQS 973
             TVE +P+IPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSR+WPSISRYRASVRTQS
Sbjct: 618  LTVEHAPAIPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSRQWPSISRYRASVRTQS 677

Query: 972  PKVEMIDNLFKPVSDTVDEGIIRELLLDFYHSSEQRKPDHIIIFRDGVSESQFNQVLNIE 793
            PKVEMIDNLFK  SDT DEGI+RE LLDFY SS +RKP+HIIIFRDGVSESQFNQVLNIE
Sbjct: 678  PKVEMIDNLFKRASDTEDEGIMREALLDFYVSSGKRKPEHIIIFRDGVSESQFNQVLNIE 737

Query: 792  LDQINEACKFLDEKWCPKFTLITAQQCQPTKFQQLG 685
            LDQI EACKFLDEKW PKFT+I AQ+   TKF Q G
Sbjct: 738  LDQIIEACKFLDEKWSPKFTVIIAQKNHHTKFFQPG 773



 Score =  158 bits (400), Expect(2) = 3e-56
 Identities = 73/94 (77%), Positives = 85/94 (90%)
 Frame = -3

Query: 325  HYHVLYDEIGFSPDDLQELVHSLSYVYQRSTTAISIVAPVCYAHLAAAQVSQFMKFDEMS 146
            HYHVL+DEIGFSPDDLQELVH+LSYVYQRSTTAIS+VAP+CYAHLAA Q+ Q+MKF++ S
Sbjct: 812  HYHVLHDEIGFSPDDLQELVHNLSYVYQRSTTAISVVAPICYAHLAATQMGQWMKFEDTS 871

Query: 145  EASSGRGGLTSAGTIPVPELPKLHENVSSSMFFC 44
            E SS RGG+T+AG + VP+LPKL E VSSSMFFC
Sbjct: 872  ETSSSRGGVTNAGPVTVPQLPKLEEKVSSSMFFC 905



 Score = 88.2 bits (217), Expect(2) = 3e-56
 Identities = 34/45 (75%), Positives = 42/45 (93%)
 Frame = -2

Query: 575 LIVAQKNHHTKFFQTGSPDNVPPGTVIDSKICHPRNNDFYMCSHA 441
           +I+AQKNHHTKFFQ G P+NVPPGT+ID+K+CHPRN DFY+C+HA
Sbjct: 758 VIIAQKNHHTKFFQPGDPNNVPPGTIIDNKVCHPRNYDFYLCAHA 802


>gb|ACZ04920.1| argonaute 4-like protein [Pelargonium x hortorum]
          Length = 933

 Score =  357 bits (915), Expect = 5e-96
 Identities = 180/217 (82%), Positives = 197/217 (90%), Gaps = 2/217 (0%)
 Frame = -1

Query: 1326 FLLCLLPERKTSAIYGPWKRKNLADFGIVTQCIAPTKVNDQYLTNVLLKINAKLGGLNA- 1150
            FLLCLLPERK S +YGPWKRKNL+++GIVTQCIAPT+VNDQYLTNVLLKINAKLGGLN+ 
Sbjct: 585  FLLCLLPERKNSELYGPWKRKNLSEYGIVTQCIAPTRVNDQYLTNVLLKINAKLGGLNSM 644

Query: 1149 -TVECSPSIPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSRRWPSISRYRASVRTQS 973
              +E SPSIPMVSKVPTII+GMDVSHGSPGQSDVPSIAAVVSSR+WPSISRYRASVRTQS
Sbjct: 645  LAIEHSPSIPMVSKVPTIIVGMDVSHGSPGQSDVPSIAAVVSSRQWPSISRYRASVRTQS 704

Query: 972  PKVEMIDNLFKPVSDTVDEGIIRELLLDFYHSSEQRKPDHIIIFRDGVSESQFNQVLNIE 793
            PKVEMID+LFK  SDT D+GI+RELLLDFY SS++RKPD IIIFRDGVSESQFNQVLNIE
Sbjct: 705  PKVEMIDSLFKKTSDTEDDGIMRELLLDFYVSSQKRKPDQIIIFRDGVSESQFNQVLNIE 764

Query: 792  LDQINEACKFLDEKWCPKFTLITAQQCQPTKFQQLGT 682
            LDQI EACKFLDEKW PKF +I AQ+   TKF Q G+
Sbjct: 765  LDQIIEACKFLDEKWNPKFVVIVAQKNHHTKFFQPGS 801



 Score =  144 bits (362), Expect(2) = 4e-53
 Identities = 68/95 (71%), Positives = 80/95 (84%), Gaps = 1/95 (1%)
 Frame = -3

Query: 325  HYHVLYDEIGFSPDDLQELVHSLSYVYQRSTTAISIVAPVCYAHLAAAQVSQFMKFDEMS 146
            HYHVL DE+GFS DDLQE VHSLSYVYQRSTTAIS+VAP+CYAHLAA Q+ QFMKF++ S
Sbjct: 839  HYHVLLDEMGFSADDLQEFVHSLSYVYQRSTTAISVVAPICYAHLAATQMGQFMKFEDTS 898

Query: 145  EASSGR-GGLTSAGTIPVPELPKLHENVSSSMFFC 44
            E SS   GG+T+AG +PV +LP+L E V+ SMFFC
Sbjct: 899  ETSSSHGGGVTTAGPVPVAQLPRLQEKVAHSMFFC 933



 Score = 92.4 bits (228), Expect(2) = 4e-53
 Identities = 38/45 (84%), Positives = 43/45 (95%)
 Frame = -2

Query: 575 LIVAQKNHHTKFFQTGSPDNVPPGTVIDSKICHPRNNDFYMCSHA 441
           +IVAQKNHHTKFFQ GSPDNVPPGTVID+K+CHPRNNDFY+C+ A
Sbjct: 785 VIVAQKNHHTKFFQPGSPDNVPPGTVIDNKVCHPRNNDFYLCAQA 829


>gb|ABC61504.1| AGO4-1, partial [Nicotiana benthamiana]
          Length = 912

 Score =  356 bits (913), Expect = 9e-96
 Identities = 178/217 (82%), Positives = 195/217 (89%), Gaps = 2/217 (0%)
 Frame = -1

Query: 1326 FLLCLLPERKTSAIYGPWKRKNLADFGIVTQCIAPTKVNDQYLTNVLLKINAKLGGLNAT 1147
            FLLCLLPERK   IYGPWKRKNLAD+GIVTQC+AP +VNDQYLTN+LLKINAKLGGLN+ 
Sbjct: 565  FLLCLLPERKNCDIYGPWKRKNLADYGIVTQCLAPGRVNDQYLTNLLLKINAKLGGLNSV 624

Query: 1146 --VECSPSIPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSRRWPSISRYRASVRTQS 973
              +E SPSIPMVSKVPT+ILGMDVSHGSPGQSDVPSIAAVVSSR+WPSISRYRASVRTQS
Sbjct: 625  LAIEHSPSIPMVSKVPTMILGMDVSHGSPGQSDVPSIAAVVSSRQWPSISRYRASVRTQS 684

Query: 972  PKVEMIDNLFKPVSDTVDEGIIRELLLDFYHSSEQRKPDHIIIFRDGVSESQFNQVLNIE 793
            PKVEMIDNLFK VSDT D+GI+RELLLDFY  S +RKP+HI+IFRDGVSESQFNQVLNIE
Sbjct: 685  PKVEMIDNLFKKVSDTEDDGIMRELLLDFYVGSGKRKPEHIVIFRDGVSESQFNQVLNIE 744

Query: 792  LDQINEACKFLDEKWCPKFTLITAQQCQPTKFQQLGT 682
            LDQ+ EACKFLDEKW PKF +I AQ+   TKF Q G+
Sbjct: 745  LDQLIEACKFLDEKWSPKFVIIVAQKNHHTKFFQAGS 781



 Score =  155 bits (392), Expect(2) = 1e-56
 Identities = 74/94 (78%), Positives = 83/94 (88%)
 Frame = -3

Query: 325  HYHVLYDEIGFSPDDLQELVHSLSYVYQRSTTAISIVAPVCYAHLAAAQVSQFMKFDEMS 146
            HYHVL DE+GFSPDDLQ+LVH+LSYVYQRSTTAISIVAPV YAHLAA QV Q+MKF++ S
Sbjct: 819  HYHVLLDEVGFSPDDLQDLVHNLSYVYQRSTTAISIVAPVSYAHLAATQVGQWMKFEDAS 878

Query: 145  EASSGRGGLTSAGTIPVPELPKLHENVSSSMFFC 44
            E SS  GGLTSAG + VP+LP+L ENVSSSMFFC
Sbjct: 879  ETSSSHGGLTSAGPVTVPQLPRLQENVSSSMFFC 912



 Score = 92.8 bits (229), Expect(2) = 1e-56
 Identities = 37/45 (82%), Positives = 43/45 (95%)
 Frame = -2

Query: 575 LIVAQKNHHTKFFQTGSPDNVPPGTVIDSKICHPRNNDFYMCSHA 441
           +IVAQKNHHTKFFQ GSPDNVPPGT+ID+K+CHPRN DFY+C+HA
Sbjct: 765 IIVAQKNHHTKFFQAGSPDNVPPGTIIDNKVCHPRNYDFYLCAHA 809


>gb|AFV15381.1| AGO4A [Solanum lycopersicum]
          Length = 909

 Score =  354 bits (908), Expect = 3e-95
 Identities = 178/214 (83%), Positives = 194/214 (90%), Gaps = 2/214 (0%)
 Frame = -1

Query: 1326 FLLCLLPERKTSAIYGPWKRKNLADFGIVTQCIAPTKVNDQYLTNVLLKINAKLGGLNA- 1150
            FLLCLLPERK   +YGPWKRKNLA++GIVTQCIAPT+VNDQY+TNVLLKINAKLGGLN+ 
Sbjct: 562  FLLCLLPERKNCDVYGPWKRKNLAEYGIVTQCIAPTRVNDQYITNVLLKINAKLGGLNSM 621

Query: 1149 -TVECSPSIPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSRRWPSISRYRASVRTQS 973
             TVE SP+IPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSR+WPSISRYRASVRTQS
Sbjct: 622  LTVEHSPAIPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSRQWPSISRYRASVRTQS 681

Query: 972  PKVEMIDNLFKPVSDTVDEGIIRELLLDFYHSSEQRKPDHIIIFRDGVSESQFNQVLNIE 793
            PKVEMIDNLFK  SDT D+GI+RE LLDFY SS +RKP+HIIIFRDGVSESQF+QVLN+E
Sbjct: 682  PKVEMIDNLFKRTSDTEDDGIMREALLDFYVSSGKRKPEHIIIFRDGVSESQFSQVLNVE 741

Query: 792  LDQINEACKFLDEKWCPKFTLITAQQCQPTKFQQ 691
            LDQI EACKFLDEKW PKF +I AQ+   TKF Q
Sbjct: 742  LDQIIEACKFLDEKWSPKFVVIVAQKNHHTKFFQ 775



 Score =  152 bits (384), Expect(2) = 7e-54
 Identities = 70/94 (74%), Positives = 82/94 (87%)
 Frame = -3

Query: 325  HYHVLYDEIGFSPDDLQELVHSLSYVYQRSTTAISIVAPVCYAHLAAAQVSQFMKFDEMS 146
            HYHVLYDE+GFS DDLQELVH+LSYVYQRSTTAIS+VAP+CYAHLAA Q+ Q+MKF++ S
Sbjct: 816  HYHVLYDELGFSADDLQELVHNLSYVYQRSTTAISVVAPICYAHLAATQMGQWMKFEDAS 875

Query: 145  EASSGRGGLTSAGTIPVPELPKLHENVSSSMFFC 44
            E SS   G+T+AG + VP+LPKL E VSSSMFFC
Sbjct: 876  ETSSSHNGVTNAGPVSVPQLPKLEEKVSSSMFFC 909



 Score = 86.3 bits (212), Expect(2) = 7e-54
 Identities = 34/45 (75%), Positives = 41/45 (91%)
 Frame = -2

Query: 575 LIVAQKNHHTKFFQTGSPDNVPPGTVIDSKICHPRNNDFYMCSHA 441
           +IVAQKNHHTKFFQ   P+NVPPGT+ID+K+CHPRN DFY+C+HA
Sbjct: 762 VIVAQKNHHTKFFQPNDPNNVPPGTIIDNKVCHPRNYDFYLCAHA 806


>gb|AFV15382.1| AGO4B [Solanum lycopersicum]
          Length = 913

 Score =  352 bits (903), Expect = 1e-94
 Identities = 177/217 (81%), Positives = 194/217 (89%), Gaps = 2/217 (0%)
 Frame = -1

Query: 1326 FLLCLLPERKTSAIYGPWKRKNLADFGIVTQCIAPTKVNDQYLTNVLLKINAKLGGLNAT 1147
            FLLCLLPERK   IYGPWKRKNLAD GIVTQC+AP +VNDQYLTN+LLKINAKLGGLN+ 
Sbjct: 566  FLLCLLPERKNCDIYGPWKRKNLADHGIVTQCLAPGRVNDQYLTNLLLKINAKLGGLNSM 625

Query: 1146 V--ECSPSIPMVSKVPTIILGMDVSHGSPGQSDVPSIAAVVSSRRWPSISRYRASVRTQS 973
            +  E SPSIPMVSKVPT+ILGMDVSHGSPGQSDVPSIAAVVSSR+WPSISRYRASVRTQS
Sbjct: 626  LAAEISPSIPMVSKVPTMILGMDVSHGSPGQSDVPSIAAVVSSRQWPSISRYRASVRTQS 685

Query: 972  PKVEMIDNLFKPVSDTVDEGIIRELLLDFYHSSEQRKPDHIIIFRDGVSESQFNQVLNIE 793
            PKVEMIDN+FK VSDT D+GI+RELLLDFY SS +RKP+HII+FRDGVSESQFNQVLNIE
Sbjct: 686  PKVEMIDNIFKKVSDTDDDGIMRELLLDFYVSSGKRKPEHIIVFRDGVSESQFNQVLNIE 745

Query: 792  LDQINEACKFLDEKWCPKFTLITAQQCQPTKFQQLGT 682
            LDQ+ EAC FLDEKW PKF +I AQ+   TKF Q G+
Sbjct: 746  LDQLIEACNFLDEKWSPKFVIIVAQKNHHTKFFQSGS 782



 Score =  152 bits (384), Expect(2) = 7e-57
 Identities = 71/94 (75%), Positives = 83/94 (88%)
 Frame = -3

Query: 325  HYHVLYDEIGFSPDDLQELVHSLSYVYQRSTTAISIVAPVCYAHLAAAQVSQFMKFDEMS 146
            HYHVL DE+GFSPD+LQELVH+LSYVYQRSTTAISIVAP+ YAHLAA QV Q+MKF++ S
Sbjct: 820  HYHVLLDEVGFSPDELQELVHNLSYVYQRSTTAISIVAPISYAHLAATQVGQWMKFEDAS 879

Query: 145  EASSGRGGLTSAGTIPVPELPKLHENVSSSMFFC 44
            E SS  GGLT+AG + VP+LP+L ENV+SSMFFC
Sbjct: 880  ETSSSHGGLTNAGPVTVPQLPRLQENVASSMFFC 913



 Score = 96.3 bits (238), Expect(2) = 7e-57
 Identities = 38/45 (84%), Positives = 45/45 (100%)
 Frame = -2

Query: 575 LIVAQKNHHTKFFQTGSPDNVPPGTVIDSKICHPRNNDFYMCSHA 441
           +IVAQKNHHTKFFQ+GSPDNVPPGT+ID+K+CHPRNNDFY+C+HA
Sbjct: 766 IIVAQKNHHTKFFQSGSPDNVPPGTIIDNKVCHPRNNDFYLCAHA 810