BLASTX nr result
ID: Coptis24_contig00009150
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00009150
(2048 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum] 879 0.0
ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus ... 845 0.0
gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum] 839 0.0
gb|AEX32786.1| Coumaroyl-CoA ligase [Vitis vinifera] 835 0.0
gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum] 835 0.0
>gb|AFG26324.1| 4-coumarate-CoA ligase [Cinnamomum osmophloeum]
Length = 565
Score = 879 bits (2271), Expect = 0.0
Identities = 447/566 (78%), Positives = 491/566 (86%), Gaps = 3/566 (0%)
Frame = -3
Query: 1929 MITIASPETKPPQINPQVSTSETENTCPETFVFRSKLPDIPISNHLPLHTYCFERLSEFS 1750
M+++ASPET+P +P T EN E F+FRSKLPDIPISNHLPLHTYCFE+++EFS
Sbjct: 1 MLSVASPETQPQIPSP---TPTPENPPVENFIFRSKLPDIPISNHLPLHTYCFEKITEFS 57
Query: 1749 DRPCLISGSDGRIYTYAETHLICQRTAVGLSSLGIKKGDVIMILLQNCPEFVFSFMGASM 1570
D PCLISGS GRIYT+AETHLICQ++A GLS LGIKKGDVIMILLQN PEF FSFMGASM
Sbjct: 58 DAPCLISGSSGRIYTFAETHLICQKSAAGLSKLGIKKGDVIMILLQNSPEFAFSFMGASM 117
Query: 1569 LGAITTTANPFYTSAEIFKQVSASGAKLIITQSLYVDKLQHSNGI---CCEDFKVVTIND 1399
+GAITTTANPF TSAEIFKQ S SGAKL+ITQS YVDKL+ + EDF V+T++D
Sbjct: 118 IGAITTTANPFCTSAEIFKQFSVSGAKLVITQSQYVDKLRDTGEKFPKIGEDFTVITVDD 177
Query: 1398 PQKNCIHFSEISNADELKIEPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISSVAQQI 1219
P ++C+HFS IS ADE +I VSIDP+D V++P+SSGTTGLPKGV LTHKSLISSVAQQ+
Sbjct: 178 PPESCLHFSVISGADEREIPSVSIDPDDPVSMPFSSGTTGLPKGVFLTHKSLISSVAQQV 237
Query: 1218 DGENPNLYLTADDTVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNLIQKHR 1039
DGENPNLYL DD VLCVLPLFHIYSLNSVLLCSLR GAGVLLM KFEI++LL LIQ+HR
Sbjct: 238 DGENPNLYLKTDDIVLCVLPLFHIYSLNSVLLCSLRAGAGVLLMHKFEIRSLLELIQRHR 297
Query: 1038 VSXXXXXXXXXXXXAKDPTVDKFDLSSIRIVLSGAAPLSKELEEALRSRVPQAIFGQGYG 859
VS AK+P V FDLSSIRIVLSGAAPL KELEEAL SR+PQAIFGQGYG
Sbjct: 298 VSVAPVVPPLVLALAKNPMVASFDLSSIRIVLSGAAPLGKELEEALHSRLPQAIFGQGYG 357
Query: 858 MTEAGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEICIRGPQ 679
MTEAGPVLSMC GFA+Q FPTKSGSCGTVVRNAELK+IDPE+G SL+ NQPGEICIRGPQ
Sbjct: 358 MTEAGPVLSMCLGFARQAFPTKSGSCGTVVRNAELKVIDPETGFSLQYNQPGEICIRGPQ 417
Query: 678 IMKGYLNDADATASTIDLDGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEA 499
IMKGYLNDA+ATASTID+DGWLHTGD+GYVDDDDEVFIVDRVKELIKFKGFQVPPAELEA
Sbjct: 418 IMKGYLNDAEATASTIDVDGWLHTGDVGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEA 477
Query: 498 LLVSHPSIADAAVVPQMDAVAGEVPVAFVVRSNDFELTEEAVKEFIAKQVVFYKRLHKVY 319
LL+SHPSIADAAVVPQ D AGEVPVAFVVRS+ FELTEEAVKEFIAKQVVFYKRLHKVY
Sbjct: 478 LLISHPSIADAAVVPQKDEAAGEVPVAFVVRSSGFELTEEAVKEFIAKQVVFYKRLHKVY 537
Query: 318 FIHAIPKSPSGKILRKDLRAKLASSS 241
F+HAIPKS SGKIL KDLRAKLA+ S
Sbjct: 538 FVHAIPKSASGKILGKDLRAKLATGS 563
>ref|XP_002520028.1| AMP dependent CoA ligase, putative [Ricinus communis]
gi|223540792|gb|EEF42352.1| AMP dependent CoA ligase,
putative [Ricinus communis]
Length = 573
Score = 845 bits (2182), Expect = 0.0
Identities = 434/571 (76%), Positives = 490/571 (85%), Gaps = 8/571 (1%)
Frame = -3
Query: 1929 MITIASPETKPPQINPQVSTSETEN---TCPETFVFRSKLPDIPISNHLPLHTYCFERLS 1759
MI+IAS E P Q S+S+ +N + ET +FRSKLPDIPISNHLPLHTYCFE L
Sbjct: 1 MISIASLEP-PKQELSSSSSSQKQNPSSSSSETHIFRSKLPDIPISNHLPLHTYCFENLF 59
Query: 1758 EFSDRPCLISGSDGRIYTYAETHLICQRTAVGLSSLGIKKGDVIMILLQNCPEFVFSFMG 1579
+ SD+PCLISGS G+ Y++AETHLI Q+TA GLSSLGIKKGDVIMILLQNCPEFVFSFMG
Sbjct: 60 KLSDKPCLISGSTGKTYSFAETHLISQKTAAGLSSLGIKKGDVIMILLQNCPEFVFSFMG 119
Query: 1578 ASMLGAITTTANPFYTSAEIFKQVSASGAKLIITQSLYVDKLQHSNGICC----EDFKVV 1411
ASM+GA+ TTANPFYTS EIFKQ+++S KLIITQSLYVDKL+ + EDF V+
Sbjct: 120 ASMIGAVATTANPFYTSNEIFKQLNSSRPKLIITQSLYVDKLRDPDHKIQPKLGEDFTVI 179
Query: 1410 TINDPQKNCIHFSEISNADELKI-EPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISS 1234
TI++P +NC+HF+ +S A E +I E ++ +P+D VALP+SSGTTGLPKGV+LTHKSLI+S
Sbjct: 180 TIDEPPENCLHFTVLSEASETEIPEVINANPDDPVALPFSSGTTGLPKGVILTHKSLITS 239
Query: 1233 VAQQIDGENPNLYLTADDTVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNL 1054
VAQQ+DGENPNLYL DD VLCVLPLFHIYSLNSVLLCSLR GA VLLMQKF+I LL L
Sbjct: 240 VAQQVDGENPNLYLKEDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFQIGALLEL 299
Query: 1053 IQKHRVSXXXXXXXXXXXXAKDPTVDKFDLSSIRIVLSGAAPLSKELEEALRSRVPQAIF 874
IQKH+VS AK+P V +FDLSSIR+VLSGAAPL KELE+ALRSRVPQAI
Sbjct: 300 IQKHKVSVAAVVPPLVLALAKNPMVAEFDLSSIRVVLSGAAPLGKELEDALRSRVPQAIL 359
Query: 873 GQGYGMTEAGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEIC 694
GQGYGMTEAGPVLSMC GFAKQPFPTKSGSCGTVVRNAELK+IDPE+G SL NQPGEIC
Sbjct: 360 GQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEIC 419
Query: 693 IRGPQIMKGYLNDADATASTIDLDGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPP 514
IRGPQIMKGYLND +ATA+TID++GWLHTGDIGYVDDDDE+FIVDRVKE+IKFKGFQVPP
Sbjct: 420 IRGPQIMKGYLNDPEATANTIDVEGWLHTGDIGYVDDDDEIFIVDRVKEIIKFKGFQVPP 479
Query: 513 AELEALLVSHPSIADAAVVPQMDAVAGEVPVAFVVRSNDFELTEEAVKEFIAKQVVFYKR 334
AELEALL++HPSIADAAVVPQ D VAGEVPVAFVVRSN FEL+EE VKE+IAKQVVFYK+
Sbjct: 480 AELEALLLNHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELSEEDVKEYIAKQVVFYKK 539
Query: 333 LHKVYFIHAIPKSPSGKILRKDLRAKLASSS 241
LHKVYFIHAIPKSPSGKILRKDL+AKLA+ S
Sbjct: 540 LHKVYFIHAIPKSPSGKILRKDLKAKLATPS 570
>gb|ACT32027.1| 4-coumarate:CoA ligase 1 [Gossypium hirsutum]
Length = 573
Score = 839 bits (2168), Expect = 0.0
Identities = 426/574 (74%), Positives = 483/574 (84%), Gaps = 8/574 (1%)
Frame = -3
Query: 1926 ITIASPETKPPQINPQVSTSETENTCPETFVFRSKLPDIPISNHLPLHTYCFERLSEFSD 1747
+ + SP KP Q++P+ P +FRSKLPDIPISNHLPLHTYCFE L SD
Sbjct: 9 VELESP--KPDQLSPET---------PADHIFRSKLPDIPISNHLPLHTYCFENLPSLSD 57
Query: 1746 RPCLISGSDGRIYTYAETHLICQRTAVGLSSLGIKKGDVIMILLQNCPEFVFSFMGASML 1567
+PCLISGS G+IY+++ETHLI Q+TA GLS+LGI+KGD IMILL NC EFVF+FMGASM+
Sbjct: 58 KPCLISGSSGKIYSFSETHLIAQKTAAGLSNLGIQKGDAIMILLHNCAEFVFAFMGASMI 117
Query: 1566 GAITTTANPFYTSAEIFKQVSASGAKLIITQSLYVDKLQHSNGI--------CCEDFKVV 1411
GA++TTANPFYTS EIFKQ A+ AKLIITQS YVDKL+ +N EDFKV+
Sbjct: 118 GAVSTTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNDGDENFPKIGEDFKVI 177
Query: 1410 TINDPQKNCIHFSEISNADELKIEPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISSV 1231
TI+DP +NC+ FS +S A+E +I V+I P+D VALP+SSGTTGLPKGV+LTHKSLI+SV
Sbjct: 178 TIDDPPENCLDFSMLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITSV 237
Query: 1230 AQQIDGENPNLYLTADDTVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNLI 1051
AQQ+DGENPNLYLT DD VLCVLPLFHIYSLNSVLLCSLR GA VLLMQKFEI TLL LI
Sbjct: 238 AQQVDGENPNLYLTHDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELI 297
Query: 1050 QKHRVSXXXXXXXXXXXXAKDPTVDKFDLSSIRIVLSGAAPLSKELEEALRSRVPQAIFG 871
Q+H+VS AK+P V +FDLSSIR+VLSGAAPL KELE+ALR RVPQA+ G
Sbjct: 298 QRHKVSVAAVVPPLVVLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEDALRGRVPQAVLG 357
Query: 870 QGYGMTEAGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEICI 691
QGYGMTEAGPVLSMC GFAKQPFPTKSGSCGTVVRNAELK+IDPE+G SL NQPGEICI
Sbjct: 358 QGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEICI 417
Query: 690 RGPQIMKGYLNDADATASTIDLDGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPA 511
RG QIMKGYLND ATA+TID++GWLHTGDIGYVD+DDE+FIVDRVKE+IKFKGFQVPPA
Sbjct: 418 RGSQIMKGYLNDGVATAATIDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPPA 477
Query: 510 ELEALLVSHPSIADAAVVPQMDAVAGEVPVAFVVRSNDFELTEEAVKEFIAKQVVFYKRL 331
ELE+LL+SHPSIADAAVVPQ D VAGEVPVAFVVRSN FELTEEAVKEFIAKQVV+YKRL
Sbjct: 478 ELESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIAKQVVYYKRL 537
Query: 330 HKVYFIHAIPKSPSGKILRKDLRAKLASSS*GKH 229
HKV+F+HAIPKSPSGKILRKDLRAKLA+++ H
Sbjct: 538 HKVHFVHAIPKSPSGKILRKDLRAKLAAAAPNTH 571
>gb|AEX32786.1| Coumaroyl-CoA ligase [Vitis vinifera]
Length = 570
Score = 835 bits (2157), Expect = 0.0
Identities = 421/559 (75%), Positives = 479/559 (85%), Gaps = 10/559 (1%)
Frame = -3
Query: 1887 NPQVSTSETENTCP------ETFVFRSKLPDIPISNHLPLHTYCFERLSEFSDRPCLISG 1726
NP VS +T ++ P + VFRSKLP+IPISNHLPLHTYCFE S+F+DRPCLI G
Sbjct: 8 NPDVSNLDTSHSIPKMANRIDDHVFRSKLPEIPISNHLPLHTYCFENYSQFADRPCLIVG 67
Query: 1725 SDGRIYTYAETHLICQRTAVGLSSLGIKKGDVIMILLQNCPEFVFSFMGASMLGAITTTA 1546
S + Y++AETHLI ++ G + LG+K+GDV+MILLQNC EF FSF+GASM+GA+TTTA
Sbjct: 68 STNKTYSFAETHLISRKVGAGFAHLGLKQGDVVMILLQNCAEFAFSFLGASMVGAVTTTA 127
Query: 1545 NPFYTSAEIFKQVSASGAKLIITQSLYVDKLQ-HSNGICC---EDFKVVTINDPQKNCIH 1378
NPFYTSAEIFKQ++AS AK+++TQ+ YVDKL+ + +G E F V+TI+DP +NC+H
Sbjct: 128 NPFYTSAEIFKQLNASKAKIVVTQAQYVDKLRDYPDGQVAKIGEGFTVITIDDPPENCMH 187
Query: 1377 FSEISNADELKIEPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISSVAQQIDGENPNL 1198
FS +S A+E ++ VSI+ +D VALP+SSGTTGLPKGV+LTHKSLI+SVAQQ+DGENPNL
Sbjct: 188 FSVVSEANESELPEVSINSDDPVALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGENPNL 247
Query: 1197 YLTADDTVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNLIQKHRVSXXXXX 1018
+LT DD VLCVLPLFHIYSLNSVLLCSLR GA VLLMQKFEI TLL LIQ++RVS
Sbjct: 248 HLTPDDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLELIQRYRVSVAAVV 307
Query: 1017 XXXXXXXAKDPTVDKFDLSSIRIVLSGAAPLSKELEEALRSRVPQAIFGQGYGMTEAGPV 838
AK+P V+ FDLSSIR+VLSGAAPL KELE ALRSRVPQA+ GQGYGMTEAGPV
Sbjct: 308 PPLVLALAKNPMVESFDLSSIRVVLSGAAPLGKELEAALRSRVPQAVLGQGYGMTEAGPV 367
Query: 837 LSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEICIRGPQIMKGYLN 658
LSMC GFAKQPFPTKSGSCGTVVRNAELK++DPE+G SL RNQPGEICIRG QIMKGYLN
Sbjct: 368 LSMCLGFAKQPFPTKSGSCGTVVRNAELKVVDPETGCSLGRNQPGEICIRGQQIMKGYLN 427
Query: 657 DADATASTIDLDGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPPAELEALLVSHPS 478
D +ATASTID+DGWLHTGDIGYVDDD+EVFIVDRVKELIKFKGFQVPPAELEALLVSHPS
Sbjct: 428 DPEATASTIDVDGWLHTGDIGYVDDDEEVFIVDRVKELIKFKGFQVPPAELEALLVSHPS 487
Query: 477 IADAAVVPQMDAVAGEVPVAFVVRSNDFELTEEAVKEFIAKQVVFYKRLHKVYFIHAIPK 298
IADAAVVPQ D VAGEVPVAFVVRSN FELTEEAVKEFI+KQVVFYKRLHKVYF+HAIPK
Sbjct: 488 IADAAVVPQKDDVAGEVPVAFVVRSNGFELTEEAVKEFISKQVVFYKRLHKVYFVHAIPK 547
Query: 297 SPSGKILRKDLRAKLASSS 241
SPSGKILRKDLRAKLA +
Sbjct: 548 SPSGKILRKDLRAKLAEKT 566
>gb|ACZ06243.1| 4-coumarate:CoA ligase 2 [Gossypium hirsutum]
Length = 574
Score = 835 bits (2157), Expect = 0.0
Identities = 425/575 (73%), Positives = 484/575 (84%), Gaps = 9/575 (1%)
Frame = -3
Query: 1926 ITIASPETKPPQINPQVSTSETENTCPETFVFRSKLPDIPISNHLPLHTYCFERLSEFSD 1747
+ + SP KP Q++P+ P +FRSKLPDIPISNHLPLHTYCFE L SD
Sbjct: 9 VELESP--KPDQLSPET---------PADHIFRSKLPDIPISNHLPLHTYCFENLPSLSD 57
Query: 1746 RPCLISGSDGRIYTYAETHLICQRTAVGLSSLGIKKGDVIMILLQNCPEFVFSFMGASML 1567
+PCLISGS G+IY+++ETHLI Q+TA GLS LGI+KGD IMILL NC EFVF+FMGASM+
Sbjct: 58 KPCLISGSSGKIYSFSETHLIAQKTAAGLSKLGIQKGDAIMILLHNCAEFVFAFMGASMI 117
Query: 1566 GAITTTANPFYTSAEIFKQVSASGAKLIITQSLYVDKLQHSN----GI-----CCEDFKV 1414
GA++TTANPFYTS EIFKQ A+ AKLIITQS YVDKL+ +N G+ EDFKV
Sbjct: 118 GAVSTTANPFYTSKEIFKQFKAARAKLIITQSQYVDKLKDTNNNNDGVENFPKIGEDFKV 177
Query: 1413 VTINDPQKNCIHFSEISNADELKIEPVSIDPNDAVALPYSSGTTGLPKGVMLTHKSLISS 1234
+TI+DP +NC+ FS +S A+E +I V+I P+D VALP+SSGTTGLPKGV+LTHKSLI+S
Sbjct: 178 ITIDDPPENCLDFSVLSEANENEIPQVTIHPDDPVALPFSSGTTGLPKGVVLTHKSLITS 237
Query: 1233 VAQQIDGENPNLYLTADDTVLCVLPLFHIYSLNSVLLCSLRRGAGVLLMQKFEIKTLLNL 1054
VAQQ+DGENPNLYLT +D VLCVLPLFHIYSLNSVLLCSLR GA VLLMQKFEI TLL L
Sbjct: 238 VAQQVDGENPNLYLTHEDVVLCVLPLFHIYSLNSVLLCSLRAGAAVLLMQKFEIGTLLEL 297
Query: 1053 IQKHRVSXXXXXXXXXXXXAKDPTVDKFDLSSIRIVLSGAAPLSKELEEALRSRVPQAIF 874
IQ+H+VS AK+P V +FDLSSIR+VLSGAAPL KELEEALR RVPQA+
Sbjct: 298 IQRHKVSVAAVVPPLVLLLAKNPVVAQFDLSSIRVVLSGAAPLGKELEEALRGRVPQAVL 357
Query: 873 GQGYGMTEAGPVLSMCSGFAKQPFPTKSGSCGTVVRNAELKIIDPESGLSLRRNQPGEIC 694
GQGYGMTEAGPVLSMC GFAKQPFPTKSGSCGTVVRNAELK+IDPE+G SL NQPGEIC
Sbjct: 358 GQGYGMTEAGPVLSMCLGFAKQPFPTKSGSCGTVVRNAELKVIDPETGCSLGYNQPGEIC 417
Query: 693 IRGPQIMKGYLNDADATASTIDLDGWLHTGDIGYVDDDDEVFIVDRVKELIKFKGFQVPP 514
IRG QIMKGYLND+ ATA+T D++GWLHTGDIGYVD+DDE+FIVDRVKE+IKFKGFQVPP
Sbjct: 418 IRGSQIMKGYLNDSVATAATTDVEGWLHTGDIGYVDEDDEIFIVDRVKEIIKFKGFQVPP 477
Query: 513 AELEALLVSHPSIADAAVVPQMDAVAGEVPVAFVVRSNDFELTEEAVKEFIAKQVVFYKR 334
AELE+LL+SHPSIADAAVVPQ D VAGEVPVAFVVRSN FELTEEAVKEFIA+QVV+YKR
Sbjct: 478 AELESLLISHPSIADAAVVPQKDEVAGEVPVAFVVRSNGFELTEEAVKEFIARQVVYYKR 537
Query: 333 LHKVYFIHAIPKSPSGKILRKDLRAKLASSS*GKH 229
LHKV+F+HAIPKSPSGKILRKDLRAKLA+++ H
Sbjct: 538 LHKVHFVHAIPKSPSGKILRKDLRAKLAAAAPTTH 572