BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00008001
         (2502 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002276470.2| PREDICTED: uncharacterized protein At5g41620...   641   0.0  
ref|XP_002509896.1| conserved hypothetical protein [Ricinus comm...   567   e-159
ref|XP_002303618.1| predicted protein [Populus trichocarpa] gi|2...   509   e-141
ref|XP_002299501.1| predicted protein [Populus trichocarpa] gi|2...   506   e-140
ref|XP_002276152.2| PREDICTED: uncharacterized protein At5g41620...   502   e-139

>ref|XP_002276470.2| PREDICTED: uncharacterized protein At5g41620-like [Vitis vinifera]
          Length = 648

 Score =  641 bits (1653), Expect = 0.0
 Identities = 370/655 (56%), Positives = 445/655 (67%), Gaps = 39/655 (5%)
 Frame = -3

Query: 2059 KLKRGVLVGKRRGPTTPSPTWKLFSPSSDQENNLNTSLTLPXXXXXXXARKLCANLWEVH 1880
            KLKRGVLVGKR GP TPSPTW+L    +D  ++++  L           RKL ANLWE+ 
Sbjct: 4    KLKRGVLVGKRGGPCTPSPTWRLGFSLNDATSSIDKDLDCSTSVSA---RKLGANLWEIQ 60

Query: 1879 -HLPVTKMSKGGGGLSRIRHYREKGLHLPTHLADPSHTPLHQV----------------- 1754
             HLPV  M++GGG L R  H+++KG  LPTHL DP H+P  Q                  
Sbjct: 61   SHLPVANMNRGGGRL-RHHHHKDKGFELPTHLVDPPHSPPDQPESASSLRRHVAASLMQH 119

Query: 1753 --------AAYYPPS------TPASSLDSKGRPGESSHSLKTSTEFLKVLNRIWSLEEQH 1616
                     A  P S      TP SSLD KGR GESS++LKTSTE LKVLNRIWSLEEQH
Sbjct: 120  HRSVERNGRALQPVSPASYSITPTSSLDFKGRIGESSYNLKTSTELLKVLNRIWSLEEQH 179

Query: 1615 ASNVSLVKALKIELHSARTRIKELLQEQQADRNEIDDLMKQIAEDKLFRKSKETDRIKST 1436
            AS +SLVKALK+EL  +R RIKELLQE+Q +R E+DDLMKQ+AEDKL RK+KE DRIK+ 
Sbjct: 180  ASTISLVKALKMELDHSRARIKELLQEKQTERQEMDDLMKQVAEDKLIRKTKEQDRIKAA 239

Query: 1435 LQSVRDELEDERKLRKRSESLHRKLARELSETKSAFSRALKELERERKARILLEDLCDEF 1256
            +QSVRDELEDERKLRKRSE+LHRKLARELSE KS+FS AL+ELERE+KARILLEDLCDEF
Sbjct: 240  VQSVRDELEDERKLRKRSETLHRKLARELSEVKSSFSNALRELEREKKARILLEDLCDEF 299

Query: 1255 ATGIGDYEQEVRALRHKNEKDQGGKDDQDRLIVHISEAWLDERLQMKLAEAQCDVSEKNT 1076
            A GI +YEQEVR+L+HK EKD+  +++ DRL++HISEAWLDER+QMKLAEA+CDV+EKNT
Sbjct: 300  AKGIREYEQEVRSLKHKPEKDRVARENSDRLVLHISEAWLDERMQMKLAEARCDVAEKNT 359

Query: 1075 IVDKLSFEIETFLKAKRSGNLNRNEISPSRDSKKHKNLRRHSLESVHFLGATSAPQDVGD 896
            IVDKLSFEIETFL+AK+S    R++ S S   +K   LRRHSLES H   A SAPQ+  D
Sbjct: 360  IVDKLSFEIETFLRAKQSVTSRRDDYS-SPSEQKESRLRRHSLESFHLNEAVSAPQNAED 418

Query: 895  EEDSVGSDSHCFELVNKNMGDKATGSISKPKLHELGEEAVEDDELGETRKPNYVKKKPGT 716
            EEDS  SDSHCFEL NK  G K +    K  +    E   ED     T K    KKK G+
Sbjct: 419  EEDSSDSDSHCFEL-NKGSGAKQSNGSCKKHIGNSAEGHAED-----TVKSYPTKKKSGS 472

Query: 715  GERTKARNPSGSKTQFEEQIKRAMSSNENKSQFLNRE--DKKGGEEAEISISQKSENCET 542
             E TK R PSG +TQFEE + R MS N NK+Q ++ E  +  G +  EI+ SQK E  E 
Sbjct: 473  QEITKGRKPSGLRTQFEEYMARTMSCNGNKTQLVDSEQGEMGGDDSVEINNSQKFEPNEA 532

Query: 541  TN----EKKLRNDGIHGSHSNNVIEGLLR-RSRMLLADGGRKEIQSEDDGGEDSGAWRGI 377
            T     EKK +  G  G + N+V++ L+R  S  L  +    E    +D G  S    G 
Sbjct: 533  TQESMPEKKNKRAGARGVNLNHVLDNLIRNHSSPLEGEKVHPENNCREDAGNQS-VLMGH 591

Query: 376  ASPVQQWVSRLAAPDLEISESSSQWAPSLKGNTLKARLMEARLEGQHSRLKASKG 212
            ASP+QQW+S+L +PDLEISESSS+W    + N+LKA+L+EARLEGQH R KASKG
Sbjct: 592  ASPIQQWMSKLTSPDLEISESSSKWPRGSRENSLKAKLLEARLEGQHFRAKASKG 646


>ref|XP_002509896.1| conserved hypothetical protein [Ricinus communis]
            gi|223549795|gb|EEF51283.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 696

 Score =  567 bits (1461), Expect = e-159
 Identities = 357/711 (50%), Positives = 453/711 (63%), Gaps = 80/711 (11%)
 Frame = -3

Query: 2104 KGRNQGAEKQENLTIKLKRGVLVGKRRGP--TTPSPTWKLFSPSS----------DQENN 1961
            K     AEK++NL  KLKR VLVGK+ GP   TP   W+  +P +          DQE  
Sbjct: 4    KSGKGAAEKEDNLGEKLKRWVLVGKKVGPFSCTPVRFWRSVAPPTAHFSIIAKHQDQEEP 63

Query: 1960 LNTSLTL-PXXXXXXXARKLCANLWEV-HHLPVTKMSKGGGGLS-------RIRH----- 1823
               S +          ARKL A LWE  H+LP++KM +G    S       R+R      
Sbjct: 64   PFVSYSNNKKAATVVSARKLAAALWEFQHYLPLSKMHRGMHNHSNGTACDPRLRRHQNRH 123

Query: 1822 ---YREKGLHLPTHLADPSHTPLHQ----------------------------------- 1757
               +++KGL L   LADPS +   Q                                   
Sbjct: 124  HHLFKDKGLDLSHFLADPSASSTDQPESAGSLRRHIAASLIQHHRSIEKSNHALQPVSPA 183

Query: 1756 -------VAAYYPPSTPASSLDSKGRPGESSHSLKTSTEFLKVLNRIWSLEEQHASNVSL 1598
                   VA Y P  TP+SSLD KGR GES +SLKTSTE LKVLNRIWSLEEQH+SN+SL
Sbjct: 184  SYGSSMEVAPYNPAVTPSSSLDFKGRIGESHYSLKTSTELLKVLNRIWSLEEQHSSNMSL 243

Query: 1597 VKALKIELHSARTRIKELLQEQQADRNEIDDLMKQIAEDKLFRKSKETDRIKSTLQSVRD 1418
            +KALK+EL  AR RIKELL++QQADR+EIDDLMKQIAEDKL RKSKE DR+ + +QS+RD
Sbjct: 244  IKALKMELDHARVRIKELLRDQQADRHEIDDLMKQIAEDKLVRKSKEQDRLHAAIQSLRD 303

Query: 1417 ELEDERKLRKRSESLHRKLARELSETKSAFSRALKELERERKARILLEDLCDEFATGIGD 1238
            E+EDERKLRKRSESLHRKLARELSE KS+ S A+KE+E+ERK+R LLEDLCDEFA GI D
Sbjct: 304  EIEDERKLRKRSESLHRKLARELSEVKSSLSNAVKEMEKERKSRKLLEDLCDEFARGIKD 363

Query: 1237 YEQEVRALRHKNEKDQGGKDDQDRLIVHISEAWLDERLQMKLAEAQCDVSEKNTIVDKLS 1058
            YEQE+ A++ K++KD  GK D DRLI+HISE+WLDER+QM+L EAQ   +E N+IVDKL 
Sbjct: 364  YEQELHAVKPKSDKDWVGKADGDRLILHISESWLDERMQMRLEEAQHGFAENNSIVDKLG 423

Query: 1057 FEIETFLKAKRSGNLNRNEISPSRDSKKHKNLRRHSLESVHFLGATSAPQDVGDEEDSVG 878
            FE+ETFLKAKR  N      S      K    RR+S+ESV    A SAPQDVGDEEDS+ 
Sbjct: 424  FELETFLKAKRVAN------SMINTDNKLPRERRNSMESVPLNEAVSAPQDVGDEEDSIS 477

Query: 877  SDSHCFELVNKNMGDKATGSISKPKLHELGEEAVEDDELGETRKPNYVKKKPGTGERTKA 698
            SDSHCFEL   + G+          LH  G+EA+ DD + ET K +  KKK  + ER + 
Sbjct: 478  SDSHCFELNKPSNGEF--------NLH--GDEAL-DDHVDETVKSSQTKKKSASHERNRR 526

Query: 697  RNPSGSKTQFEEQIKRAMSSN-ENKSQFLNREDKKGGE--EAEISISQKSENCETTN--- 536
            +NPS  + +FEEQ+  AM++N  NK   +  E+ K GE   AE+SIS++SENC+TT    
Sbjct: 527  QNPSSLQVKFEEQMAWAMAANGNNKFPVVGIEELKTGEGNPAEMSISRRSENCDTTEGGS 586

Query: 535  -EKKLRNDGIHGSHSNNVIEGLLRRSRMLLADGGRKEIQSEDDGGEDSGAW--RGIASPV 365
             E+K + D IHG + N VI+ L+ RS +  ++ G   ++ E+D GE S ++  R  ASPV
Sbjct: 587  IERKKKVDEIHGMNQNYVIDNLI-RSHISSSEAG--HVRLENDAGEASTSYPTRRNASPV 643

Query: 364  QQWVSRLAAPDLEISESSSQWAPSLKGNTLKARLMEARLEGQHSRLKASKG 212
            +QW+++L+ PDL+ISESS++   +LK NTLKA+L+EAR +GQ SRLK  +G
Sbjct: 644  RQWMAKLSTPDLDISESSTKQPSTLKENTLKAKLLEARSKGQRSRLKIFRG 694


>ref|XP_002303618.1| predicted protein [Populus trichocarpa] gi|222841050|gb|EEE78597.1|
            predicted protein [Populus trichocarpa]
          Length = 593

 Score =  509 bits (1310), Expect = e-141
 Identities = 314/626 (50%), Positives = 391/626 (62%), Gaps = 59/626 (9%)
 Frame = -3

Query: 1912 RKLCANLWEV-HHLP-VTKMSKG-----GGGLSRIRHY-------REKGLHLPTHLADPS 1775
            RKL A LWE  H+LP ++KM +G     GGG SR+R +       ++KGL L   LADP+
Sbjct: 8    RKLAAALWEFNHYLPSLSKMHRGVHINNGGGDSRLRRHEKRHHFLKDKGLDLSHFLADPT 67

Query: 1774 HTPLHQ------------------------------------------VAAYYPPSTPAS 1721
             T   Q                                          VA Y P  TP+S
Sbjct: 68   PTSPDQPESAGSLRRHIASSLIQHHRSIERSNRALQPVSPASYGSSMEVAPYNPAVTPSS 127

Query: 1720 SLDSKGRPGESSHSLKTSTEFLKVLNRIWSLEEQHASNVSLVKALKIELHSARTRIKELL 1541
            SLD KGR GES +SLKTSTE LKVLNRIWSLEEQHASN+SL+KALK EL  AR RIKELL
Sbjct: 128  SLDFKGRIGESHYSLKTSTELLKVLNRIWSLEEQHASNMSLIKALKTELDRARVRIKELL 187

Query: 1540 QEQQADRNEIDDLMKQIAEDKLFRKSKETDRIKSTLQSVRDELEDERKLRKRSESLHRKL 1361
            ++QQA+R+E+DD+MKQIAEDKL RKSKE DR+ + +QS+RDELED+RKLRKRSESL RKL
Sbjct: 188  RDQQAERHEVDDIMKQIAEDKLARKSKEQDRLHAAIQSLRDELEDDRKLRKRSESLRRKL 247

Query: 1360 ARELSETKSAFSRALKELERERKARILLEDLCDEFATGIGDYEQEVRALRHKNEKDQGGK 1181
            ARELSE KS+FS ALK++ERERK+R LLEDLCDEFA GI DYE EV AL  K+++D  G+
Sbjct: 248  ARELSEVKSSFSNALKDMERERKSRKLLEDLCDEFAKGIKDYEHEVHALNKKSDRDWVGR 307

Query: 1180 DDQDRLIVHISEAWLDERLQMKLAEAQCDVSEKNTIVDKLSFEIETFLKAKRSGNLNRNE 1001
            DD D LI+HISE WLDER+QM+L EAQ   SE N+IVDKL FEIETFLK K  GN    E
Sbjct: 308  DDGDHLILHISETWLDERMQMRLEEAQHGFSENNSIVDKLGFEIETFLKTKGMGNFKCRE 367

Query: 1000 ISPSRDSKKHKNLRRHSLESVHFLGATSAPQDVGDEEDSVGSDSHCFELVNKNMGDKATG 821
                         RR+S+ESV      SAPQDVGDEEDS GSDSHCFEL   + GD    
Sbjct: 368  -------------RRNSMESVPLNEVVSAPQDVGDEEDSTGSDSHCFELNKPSNGD---- 410

Query: 820  SISKPKLHELGEEAVEDDELGETRKPNYVKKKPGTGERTKARNPSGSKTQFEEQIKRAMS 641
                   H   +EAV      E  K    KKK  + ERT+ +NP+  + +FEE + +AM+
Sbjct: 411  ------FHLHKDEAVA-GHTEEMDKSALTKKKLASLERTRGQNPANLQVKFEEHMAQAMT 463

Query: 640  SNEN-KSQFLNREDKKGGEEAEISISQKSENCETTNEKKLRNDGIHGSHSNNVIEGLLRR 464
             N N KSQ ++ E+ K GE   +             E+K + + IHGS+SN VI+  ++ 
Sbjct: 464  YNGNKKSQVMDLEEVKTGEGGSL-------------ERKSKPEEIHGSNSNYVIDNSIKS 510

Query: 463  SRMLLADGGRKEIQSEDDGGEDSGAW--RGIASPVQQWVSRLAAPDLEISESSSQWAPSL 290
                ++      +  E+D GE S ++  R  ASPV++W+S+L   DL+ISE S++   +L
Sbjct: 511  H---ISSSEAVNLHLENDAGEASCSYPPRRNASPVRRWMSKLTTSDLDISEPSTKQPTNL 567

Query: 289  KGNTLKARLMEARLEGQHSRLKASKG 212
            K NTLKA+L+EAR +G  SRLK  KG
Sbjct: 568  KENTLKAKLLEARSKGSRSRLKIFKG 593


>ref|XP_002299501.1| predicted protein [Populus trichocarpa] gi|222846759|gb|EEE84306.1|
            predicted protein [Populus trichocarpa]
          Length = 576

 Score =  506 bits (1302), Expect = e-140
 Identities = 299/548 (54%), Positives = 368/548 (67%), Gaps = 6/548 (1%)
 Frame = -3

Query: 1837 SRIRHYR--EKGLHLPTHLADPSHTPLHQVAAYYPPSTPASSLDSKGRPGESSHSLKTST 1664
            S I+H+R  E+  H    ++  S+    +VA Y P  TP+SSLD KGR GES +SLKTST
Sbjct: 64   SLIQHHRSIERSNHALQPVSPASYGSSMEVAPYNPAVTPSSSLDFKGRIGESHYSLKTST 123

Query: 1663 EFLKVLNRIWSLEEQHASNVSLVKALKIELHSARTRIKELLQEQQADRNEIDDLMKQIAE 1484
            E LKVLNRIWSLEEQH SNVSL+KALKIEL  AR RIKELL+ QQADR+EIDDLMKQIAE
Sbjct: 124  ELLKVLNRIWSLEEQHVSNVSLIKALKIELDHARIRIKELLRYQQADRHEIDDLMKQIAE 183

Query: 1483 DKLFRKSKETDRIKSTLQSVRDELEDERKLRKRSESLHRKLARELSETKSAFSRALKELE 1304
            DKL +KSKE +R+ + +QS+RDELEDERKLRKRSESLHRKLARELSE KS+FS ALKE+E
Sbjct: 184  DKLVQKSKEQERLHAAIQSLRDELEDERKLRKRSESLHRKLARELSEVKSSFSNALKEME 243

Query: 1303 RERKARILLEDLCDEFATGIGDYEQEVRALRHKNEKDQGGKDDQDRLIVHISEAWLDERL 1124
            RERK+R LLEDLCDEFA GI DYEQEV  L  K+++D   + D DRLI+HISE+WLDER+
Sbjct: 244  RERKSRKLLEDLCDEFAKGIKDYEQEVHTLNKKSDRDWVRRADGDRLILHISESWLDERM 303

Query: 1123 QMKLAEAQCDVSEKNTIVDKLSFEIETFLKAKRSGN-LNRNEISPSRDSKKHKNLRRHSL 947
            QM+L EAQ   SE N+IVDKL FEIETFLK KR GN  + N + P          RR+S+
Sbjct: 304  QMRLEEAQHGFSENNSIVDKLGFEIETFLKTKRMGNSKSSNNVLPRE--------RRNSM 355

Query: 946  ESVHFLGATSAPQDVGDEEDSVGSDSHCFELVNKNMGDKATGSISKPKLHELGEEAVEDD 767
            ESV      SAPQDVGDE+DS GSDSHCF+       D           H  G+EAV   
Sbjct: 356  ESVPLNETVSAPQDVGDEQDSTGSDSHCFKHNRPGNDD----------FHLHGDEAVA-G 404

Query: 766  ELGETRKPNYVKKKPGTGERTKARNPSGSKTQFEEQIKRAMSSNEN-KSQFLNREDKKGG 590
               E  K    KKK  + ER++ +NPS  + +FEE   +AM  N N KS+ +  E  K G
Sbjct: 405  HTDEMEKSTRTKKKLASRERSRCQNPSNLQVKFEEHKAQAMPCNGNKKSEVMELEGVKTG 464

Query: 589  EEAEISISQKSENCETTNEKKLRNDGIHGSHSNNVIEGLLRRSRMLLADGGRKEIQSEDD 410
                              E+K + D IHGS+SN VI+ L+R     ++      +  E+D
Sbjct: 465  GSL---------------ERKSKVDEIHGSNSNYVIDNLIRSH---ISSSEAVNLHLEND 506

Query: 409  GGEDSGAW--RGIASPVQQWVSRLAAPDLEISESSSQWAPSLKGNTLKARLMEARLEGQH 236
             GE S ++  R  ASPV+QW+S+L +PDL+ISESS++  P+LK NTLKA+L+EAR +G  
Sbjct: 507  AGEASCSYPPRRYASPVRQWMSKLTSPDLDISESSTKPPPNLKENTLKAKLLEARSKGPR 566

Query: 235  SRLKASKG 212
            SRL+  KG
Sbjct: 567  SRLRIFKG 574


>ref|XP_002276152.2| PREDICTED: uncharacterized protein At5g41620-like [Vitis vinifera]
          Length = 602

 Score =  502 bits (1292), Expect = e-139
 Identities = 312/614 (50%), Positives = 385/614 (62%), Gaps = 66/614 (10%)
 Frame = -3

Query: 1861 MSKGGGGLS-------RIRH-----YREKGLHLPTHLADPSHTPLHQVAA---------- 1748
            M +GGGG         ++RH     YR+KGL LPT L DP  T   Q A+          
Sbjct: 1    MHRGGGGGGPPPPPPLKLRHRHHLHYRDKGLDLPTLLTDPFPTSPDQPASASSLRRHVAT 60

Query: 1747 --------------------------------YYPPSTPASSLDSKGRPGESSHSLKTST 1664
                                            Y P  TP SSLD KG  GESS+SLKTST
Sbjct: 61   TLMQHRRANERNSRALQPVSPASYGSSLEVGPYNPAVTPTSSLDFKGGIGESSYSLKTST 120

Query: 1663 EFLKVLNRIWSLEEQHASNVSLVKALKIELHSARTRIKELLQEQQADRNEIDDLMKQIAE 1484
            + LKVLNRIWSLEEQHASNVSL+KALK+EL  AR RIK LL++QQA+R+EIDDLMKQ+ E
Sbjct: 121  DLLKVLNRIWSLEEQHASNVSLIKALKMELGHARARIKRLLRDQQAERHEIDDLMKQV-E 179

Query: 1483 DKLFRK-SKETDRIKSTLQSVRDELEDERKLRKRSESLHRKLARELSETKSAFSRALKEL 1307
            DKL RK SKE DR+ S +QSVRDELE+ERKLRK SESLHRKLARELSE KS+FS ALKEL
Sbjct: 180  DKLLRKSSKEQDRVNSAVQSVRDELENERKLRKHSESLHRKLARELSEVKSSFSNALKEL 239

Query: 1306 ERERKARILLEDLCDEFATGIGDYEQEVRALRHKNEKDQGGKDDQDRLIVHISEAWLDER 1127
            E+ERK+R LLEDLCDEFA GI DY+QEV AL+ K++ D  G+ D DRLI+H+SE+WLDER
Sbjct: 240  EKERKSRELLEDLCDEFAKGIRDYQQEVHALKQKSDSDWAGRADHDRLILHLSESWLDER 299

Query: 1126 LQMKLAEAQCDVSEKNTIVDKLSFEIETFLKAKRSGNLNRNEISPSRDSKKHKNLRRHSL 947
            +Q KL E Q   +E N I+DKLSFEIETFL+AK+      N     R+  K + LRR+SL
Sbjct: 300  MQTKLVETQLGSAENNPILDKLSFEIETFLQAKQMHTSKTNSNMLPREPTKERYLRRNSL 359

Query: 946  ESVHFLGATSAPQDVGDEEDSVGSDSHCFELVNKNMGDKATGSISKPKLHELGEEAVEDD 767
            ESV    A SAPQD GDEE S GSD++CFEL      +K T S  KP           D+
Sbjct: 360  ESVPLHDAVSAPQDAGDEEASAGSDTNCFEL------NKPTTSNFKPH---------GDE 404

Query: 766  ELGETRKPNYVKKKPGTGERTKARNPSGSKTQFEEQIKRAMSSNENKSQFLNREDKK--- 596
                T +    KKK    ER K RNPS  + +FEEQ+ RAM+ N NK+  +   D++   
Sbjct: 405  PEAHTDRMIKSKKKLVPHERIKGRNPSSLQVKFEEQMARAMACNGNKTTQVVDTDQQRKI 464

Query: 595  -GGEEAEISISQKSENCETTN----EKKLRNDGIHGSHSNNVIEGLLRRSRMLLADGGRK 431
              G   E SI+ K ENCE T     E+K+++D I    + N     L RS+ L ++ G  
Sbjct: 465  SEGIPLEASITPKPENCEATEDRSYERKIKHDAIIQEPNLNYDIDSLIRSQYLSSEAGNI 524

Query: 430  EIQSEDDGGEDS---GAWRGIASPVQQWVSRLAAPDLEISESSSQWAPSLKGNTLKARLM 260
              +++DD  E S    AWR   SPV++W+ +L +PDL+I ESSS+ AP  K NTLKA+L+
Sbjct: 525  RPENDDDCCEASFGNSAWRSHGSPVREWMVKLTSPDLDIPESSSKLAP--KENTLKAKLL 582

Query: 259  EARLEGQHSRLKAS 218
            EARL+G+ SRLKA+
Sbjct: 583  EARLKGKRSRLKAT 596