BLASTX nr result
ID: Coptis24_contig00007486
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00007486
(2297 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002267958.1| PREDICTED: 3-epi-6-deoxocathasterone 23-mono... 376 e-158
ref|XP_002310278.1| predicted protein [Populus trichocarpa] gi|2... 368 e-158
emb|CAN73509.1| hypothetical protein VITISV_007910 [Vitis vinifera] 377 e-157
ref|NP_568002.1| 3-epi-6-deoxocathasterone 23-monooxygenase [Ara... 343 e-150
dbj|BAA37167.1| cytochrome P450 [Arabidopsis thaliana] 343 e-150
>ref|XP_002267958.1| PREDICTED: 3-epi-6-deoxocathasterone 23-monooxygenase [Vitis
vinifera] gi|297735073|emb|CBI17435.3| unnamed protein
product [Vitis vinifera]
Length = 486
Score = 376 bits (966), Expect(2) = e-158
Identities = 187/312 (59%), Positives = 237/312 (75%), Gaps = 10/312 (3%)
Frame = +2
Query: 641 GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 820
GWP IGETL FIASG+SS+P+SFM+KRKS YGKVFK+++LG P+IVS DPEVNKVVLQN
Sbjct: 40 GWPVIGETLDFIASGWSSRPVSFMEKRKSRYGKVFKTNILGKPVIVSTDPEVNKVVLQNI 99
Query: 821 GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1000
GN FIP YP++I E+ G+SSILQM+G++ K+VH L+GGFL+SPQLKARIT++IER V ++
Sbjct: 100 GNVFIPAYPQTITEILGESSILQMNGSLQKRVHALLGGFLRSPQLKARITRDIERYVKLT 159
Query: 1001 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1180
++SW+D+ ++Y+Q+E + ITF VLV+VL+ + PGEDL F+KREF E IKGL+ LPIKLPG
Sbjct: 160 LDSWKDQHIVYVQDEVRKITFDVLVRVLVSITPGEDLNFMKREFAEVIKGLICLPIKLPG 219
Query: 1181 TRXXXXXXXXXXXXXXVNRIVEEKKLKM-----EVMTGDVMDAMEMLLSDAGD-----YI 1330
TR V RIVEE+K M E + G DA+++LL D GD +
Sbjct: 220 TRLYKSLKAKERLLKLVRRIVEERKAAMDRGDHETLKGPPNDAVDVLLRDTGDSSETSRL 279
Query: 1331 PFDSIGENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGEDYL 1510
D I N++EMMIPGEE++P MTLA+K+LSDCP+AL QL EENM+LK K GEDY
Sbjct: 280 SLDFISSNMIEMMIPGEETLPTAMTLAVKFLSDCPVALNQLMEENMELKKGKALSGEDYA 339
Query: 1511 WTDYMSLPFTQN 1546
WTDYM LPFTQN
Sbjct: 340 WTDYMCLPFTQN 351
Score = 211 bits (537), Expect(2) = e-158
Identities = 96/131 (73%), Positives = 116/131 (88%)
Frame = +3
Query: 1548 ETLRIANIINAVWRKAIKDVEIKGYLIPKGWCMQAYFSSVHMDEDNYENPYQFDPWRWQT 1727
ETLR+ANIINAVWRKA+KDV+IK YLIP+GW + A F+S+HMDE+NYENPYQF+PWRW+
Sbjct: 355 ETLRMANIINAVWRKAVKDVKIKDYLIPEGWGVMASFTSIHMDEENYENPYQFNPWRWE- 413
Query: 1728 KAGHVNNYNTCFTPFGGGQRLCPGLEFSRLEVSIFLHHFVTSYRWVAEEDSIVSFPTVKM 1907
KA VNN N +TPFGGGQRLCPGLE S+LE+SIFLHH VT+YRWVA++D +V FPTVKM
Sbjct: 414 KAASVNN-NNSYTPFGGGQRLCPGLELSKLEISIFLHHLVTTYRWVAKKDDVVYFPTVKM 472
Query: 1908 KRKLPIRVTPV 1940
++KLPI VTP+
Sbjct: 473 RKKLPITVTPL 483
>ref|XP_002310278.1| predicted protein [Populus trichocarpa] gi|222853181|gb|EEE90728.1|
predicted protein [Populus trichocarpa]
Length = 463
Score = 368 bits (944), Expect(2) = e-158
Identities = 186/314 (59%), Positives = 231/314 (73%), Gaps = 12/314 (3%)
Frame = +2
Query: 641 GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 820
GWP IGETL FIA+GY+SQP+SFM+KR+SLYGKVFK+H+LG PIIVS DP VNKVVLQNH
Sbjct: 14 GWPLIGETLDFIAAGYTSQPVSFMEKRRSLYGKVFKTHILGTPIIVSTDPAVNKVVLQNH 73
Query: 821 GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1000
GN FIP YPKS++E+FG+ SILQ +G + KKVH LIGGFL+SPQ K RITK+IE V ++
Sbjct: 74 GNIFIPAYPKSVREIFGEYSILQTNGTLQKKVHALIGGFLRSPQFKTRITKDIEHHVKLT 133
Query: 1001 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1180
+ SW+D L+++QEET+ TF VLVK L+ +DP +DL+FLKREF EFIKGL+ LPIKLPG
Sbjct: 134 LTSWKDLPLLFVQEETQKFTFKVLVKALLSLDPSDDLDFLKREFDEFIKGLICLPIKLPG 193
Query: 1181 TRXXXXXXXXXXXXXXVNRIVEEKKLKMEVMTGD------VMDAMEMLLSDAG------D 1324
TR V +IVE++KL +E DA+++LL D
Sbjct: 194 TRLYKSLKAKERIFKLVKKIVEQRKLGLEKSEDQEHQNTIPNDAVDVLLRDISGEQNDKQ 253
Query: 1325 YIPFDSIGENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGED 1504
+ D I +I+E+MIPGEE+VPM MTLA+K+LSDCP+AL+ L EENM LK QK G+D
Sbjct: 254 SLSLDFIISHIIELMIPGEETVPMSMTLAVKFLSDCPVALELLTEENMKLKRQKTDSGDD 313
Query: 1505 YLWTDYMSLPFTQN 1546
Y WTDYMSL FTQN
Sbjct: 314 YCWTDYMSLQFTQN 327
Score = 218 bits (555), Expect(2) = e-158
Identities = 97/131 (74%), Positives = 113/131 (86%)
Frame = +3
Query: 1548 ETLRIANIINAVWRKAIKDVEIKGYLIPKGWCMQAYFSSVHMDEDNYENPYQFDPWRWQT 1727
ETLR+ANIINAVWRKA+KDVEIKG LIP+GWC+ FSSVHMDE+NYENP++FDPWRW+
Sbjct: 331 ETLRVANIINAVWRKALKDVEIKGILIPEGWCVLTSFSSVHMDEENYENPHEFDPWRWEK 390
Query: 1728 KAGHVNNYNTCFTPFGGGQRLCPGLEFSRLEVSIFLHHFVTSYRWVAEEDSIVSFPTVKM 1907
V N CFTPFGGGQRLCPGLE SRLE+SIFLHH VT+YRW+AE+D I+ FPTVK+
Sbjct: 391 TGASVK--NNCFTPFGGGQRLCPGLELSRLEISIFLHHLVTTYRWIAEKDDIIYFPTVKL 448
Query: 1908 KRKLPIRVTPV 1940
K+KLPIRVT +
Sbjct: 449 KKKLPIRVTSI 459
>emb|CAN73509.1| hypothetical protein VITISV_007910 [Vitis vinifera]
Length = 501
Score = 377 bits (967), Expect(2) = e-157
Identities = 187/312 (59%), Positives = 237/312 (75%), Gaps = 10/312 (3%)
Frame = +2
Query: 641 GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 820
GWP IGETL FIASG+SS+P+SFM+KRKS YGKVFK+++LG P+IVS DPEVNKVVLQN
Sbjct: 40 GWPVIGETLDFIASGWSSRPVSFMEKRKSRYGKVFKTNILGKPVIVSTDPEVNKVVLQNI 99
Query: 821 GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1000
GN FIP YP++I E+ G+SSILQM+G++ K+VH L+GGFL+SPQLKARIT++IER V ++
Sbjct: 100 GNXFIPAYPQTITEILGESSILQMNGSLQKRVHALLGGFLRSPQLKARITRDIERYVKLT 159
Query: 1001 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1180
++SW+D+ ++Y+Q+E + ITF VLV+VL+ + PGEDL F+KREF E IKGL+ LPIKLPG
Sbjct: 160 LDSWKDQHIVYVQDEVRKITFDVLVRVLVSITPGEDLNFMKREFAEVIKGLICLPIKLPG 219
Query: 1181 TRXXXXXXXXXXXXXXVNRIVEEKKLKM-----EVMTGDVMDAMEMLLSDAGD-----YI 1330
TR V RIVEE+K M E + G DA+++LL D GD +
Sbjct: 220 TRLYKSLKAKERLLKLVRRIVEERKAAMDRGDHETLKGPPNDAVDVLLRDTGDSSETSRL 279
Query: 1331 PFDSIGENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGEDYL 1510
D I N++EMMIPGEE++P MTLA+K+LSDCP+AL QL EENM+LK K GEDY
Sbjct: 280 SLDFISSNMIEMMIPGEETLPTAMTLAVKFLSDCPVALNQLMEENMELKKGKALSGEDYA 339
Query: 1511 WTDYMSLPFTQN 1546
WTDYM LPFTQN
Sbjct: 340 WTDYMCLPFTQN 351
Score = 209 bits (531), Expect(2) = e-157
Identities = 95/131 (72%), Positives = 115/131 (87%)
Frame = +3
Query: 1548 ETLRIANIINAVWRKAIKDVEIKGYLIPKGWCMQAYFSSVHMDEDNYENPYQFDPWRWQT 1727
ETLR+ANIINAVWRKA+KDV+IK YLIP+GW + A F+S+HMDE+NYENPYQF+PWRW+
Sbjct: 370 ETLRMANIINAVWRKAVKDVKIKDYLIPEGWGVMASFTSIHMDEENYENPYQFNPWRWE- 428
Query: 1728 KAGHVNNYNTCFTPFGGGQRLCPGLEFSRLEVSIFLHHFVTSYRWVAEEDSIVSFPTVKM 1907
KA VNN N +TPFGGGQRLCPGLE S+LE+SIFLHH VT+Y WVA++D +V FPTVKM
Sbjct: 429 KAASVNN-NNSYTPFGGGQRLCPGLELSKLEISIFLHHLVTTYXWVAKKDDVVYFPTVKM 487
Query: 1908 KRKLPIRVTPV 1940
++KLPI VTP+
Sbjct: 488 RKKLPITVTPL 498
>ref|NP_568002.1| 3-epi-6-deoxocathasterone 23-monooxygenase [Arabidopsis thaliana]
gi|150421525|sp|Q9M066.3|C90C1_ARATH RecName:
Full=3-epi-6-deoxocathasterone 23-monooxygenase; AltName:
Full=Cytochrome P450 90C1; AltName: Full=Protein
ROTUNDIFOLIA 3 gi|115646893|gb|ABJ17155.1| At4g36380
[Arabidopsis thaliana] gi|332661249|gb|AEE86649.1|
3-epi-6-deoxocathasterone 23-monooxygenase [Arabidopsis
thaliana]
Length = 524
Score = 343 bits (881), Expect(2) = e-150
Identities = 172/306 (56%), Positives = 223/306 (72%), Gaps = 4/306 (1%)
Frame = +2
Query: 641 GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 820
GWP IGETL+FIA GYSS+P++FMDKRKSLYGKVFK++++G PII+S D EVNKVVLQNH
Sbjct: 75 GWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNKVVLQNH 134
Query: 821 GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1000
GN+F+P YPKSI EL G++SIL ++G K++H LIG FL+SP LK RIT++IE V ++
Sbjct: 135 GNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIEASVVLT 194
Query: 1001 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1180
+ SW L+++Q+E K +TF +LVKVLM PGED+ LK EF EFIKGL+ +PIK PG
Sbjct: 195 LASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICIPIKFPG 254
Query: 1181 TRXXXXXXXXXXXXXXVNRIVEEKKLKMEVMTGDVMDAMEMLLSDAGD----YIPFDSIG 1348
TR V ++VEE+++ M T D +++LL D GD P D +
Sbjct: 255 TRLYKSLKAKERLIKMVKKVVEERQVAM-TTTSPANDVVDVLLRDGGDSEKQSQPSDFVS 313
Query: 1349 ENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGEDYLWTDYMS 1528
IVEMMIPGEE++P MTLA+K+LSD P+AL +L EENM++K +K LGE+Y WTDYMS
Sbjct: 314 GKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKWTDYMS 373
Query: 1529 LPFTQN 1546
L FTQN
Sbjct: 374 LSFTQN 379
Score = 217 bits (553), Expect(2) = e-150
Identities = 101/131 (77%), Positives = 110/131 (83%)
Frame = +3
Query: 1548 ETLRIANIINAVWRKAIKDVEIKGYLIPKGWCMQAYFSSVHMDEDNYENPYQFDPWRWQT 1727
ETLR+ANIIN VWRKA+KDVEIKGYLIPKGWC+ A F SVHMDED Y+NPYQFDPWRW
Sbjct: 383 ETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWRWDR 442
Query: 1728 KAGHVNNYNTCFTPFGGGQRLCPGLEFSRLEVSIFLHHFVTSYRWVAEEDSIVSFPTVKM 1907
G N+ + CFTPFGGGQRLCPGLE S+LE+SIFLHH VT Y W AEED IVSFPTVKM
Sbjct: 443 INGSANS-SICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWTAEEDEIVSFPTVKM 501
Query: 1908 KRKLPIRVTPV 1940
KR+LPIRV V
Sbjct: 502 KRRLPIRVATV 512
>dbj|BAA37167.1| cytochrome P450 [Arabidopsis thaliana]
Length = 524
Score = 343 bits (881), Expect(2) = e-150
Identities = 172/306 (56%), Positives = 223/306 (72%), Gaps = 4/306 (1%)
Frame = +2
Query: 641 GWPFIGETLHFIASGYSSQPLSFMDKRKSLYGKVFKSHLLGLPIIVSIDPEVNKVVLQNH 820
GWP IGETL+FIA GYSS+P++FMDKRKSLYGKVFK++++G PII+S D EVNKVVLQNH
Sbjct: 75 GWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNKVVLQNH 134
Query: 821 GNSFIPYYPKSIQELFGKSSILQMSGNVHKKVHGLIGGFLKSPQLKARITKEIERCVGIS 1000
GN+F+P YPKSI EL G++SIL ++G K++H LIG FL+SP LK RIT++IE V ++
Sbjct: 135 GNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIEASVVLT 194
Query: 1001 MESWRDKELIYMQEETKDITFPVLVKVLMGVDPGEDLEFLKREFGEFIKGLVSLPIKLPG 1180
+ SW L+++Q+E K +TF +LVKVLM PGED+ LK EF EFIKGL+ +PIK PG
Sbjct: 195 LASWAQLPLVHVQDEIKKMTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLICIPIKFPG 254
Query: 1181 TRXXXXXXXXXXXXXXVNRIVEEKKLKMEVMTGDVMDAMEMLLSDAGD----YIPFDSIG 1348
TR V ++VEE+++ M T D +++LL D GD P D +
Sbjct: 255 TRLYKSLKAKERLIKMVKKVVEERQVAM-TTTSPANDVVDVLLRDGGDSEKQSQPSDFVS 313
Query: 1349 ENIVEMMIPGEESVPMLMTLAIKYLSDCPLALQQLKEENMDLKTQKRHLGEDYLWTDYMS 1528
IVEMMIPGEE++P MTLA+K+LSD P+AL +L EENM++K +K LGE+Y WTDYMS
Sbjct: 314 GKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYKWTDYMS 373
Query: 1529 LPFTQN 1546
L FTQN
Sbjct: 374 LSFTQN 379
Score = 217 bits (553), Expect(2) = e-150
Identities = 101/131 (77%), Positives = 110/131 (83%)
Frame = +3
Query: 1548 ETLRIANIINAVWRKAIKDVEIKGYLIPKGWCMQAYFSSVHMDEDNYENPYQFDPWRWQT 1727
ETLR+ANIIN VWRKA+KDVEIKGYLIPKGWC+ A F SVHMDED Y+NPYQFDPWRW
Sbjct: 383 ETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDEDIYDNPYQFDPWRWDR 442
Query: 1728 KAGHVNNYNTCFTPFGGGQRLCPGLEFSRLEVSIFLHHFVTSYRWVAEEDSIVSFPTVKM 1907
G N+ + CFTPFGGGQRLCPGLE S+LE+SIFLHH VT Y W AEED IVSFPTVKM
Sbjct: 443 INGSANS-SICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSWTAEEDEIVSFPTVKM 501
Query: 1908 KRKLPIRVTPV 1940
KR+LPIRV V
Sbjct: 502 KRRLPIRVATV 512