BLASTX nr result
ID: Coptis24_contig00007109
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00007109
(2004 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002300673.1| predicted protein [Populus trichocarpa] gi|2... 591 e-166
ref|XP_002284797.2| PREDICTED: tRNA pseudouridine synthase A, mi... 578 e-162
emb|CBI18897.3| unnamed protein product [Vitis vinifera] 565 e-158
ref|XP_003610360.1| tRNA pseudouridine synthase [Medicago trunca... 565 e-158
ref|XP_003610356.1| tRNA pseudouridine synthase [Medicago trunca... 565 e-158
>ref|XP_002300673.1| predicted protein [Populus trichocarpa] gi|222842399|gb|EEE79946.1|
predicted protein [Populus trichocarpa]
Length = 429
Score = 591 bits (1524), Expect = e-166
Identities = 293/405 (72%), Positives = 335/405 (82%), Gaps = 9/405 (2%)
Frame = +2
Query: 173 QKPRYKRRKIAIFLAYCGVGYQGMQKNPGAKTIEGELEEALYLSGAIPEHDRGKPKRFDW 352
+K RYKRRKIAIF AYCGVGYQGMQKNPGAKTIEG+LEEAL+ +GA+PE DRG PKR+DW
Sbjct: 10 KKQRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEGDLEEALFHAGAVPEQDRGIPKRYDW 69
Query: 353 ARSARTDKGVSAVGQVVSGRFYVDPPGFIDRFNSNLPNQIRIFGFNRVTSSFSAKKFCDR 532
ARSARTDKGVSAVGQVVSGRFY+DPPG ++R +SNL Q RIFG+ RVT SF+AKKFCDR
Sbjct: 70 ARSARTDKGVSAVGQVVSGRFYIDPPGLVERLHSNLSPQFRIFGYKRVTGSFNAKKFCDR 129
Query: 533 RRYVYLIPVFALDRNAHRDRESVLASLGSDNELVKCLECSERGRKVLGAMGKHY------ 694
RRYVYLIPVFALD +HRDRESVLASL S +EL+KCLECSERGRKV GA+GK
Sbjct: 130 RRYVYLIPVFALDPCSHRDRESVLASLRSGSELIKCLECSERGRKVAGAVGKRSFELTDN 189
Query: 695 SGAKIXXXXXXXXXXXXXXXMASVECGNGDE---KGVLENGSGFSYGEKEKERFNRILSH 865
S + SVE G+ ++ K + GS F YGEKEKERFNRIL +
Sbjct: 190 SSNDKDASVKSEIKEEVDEIIVSVENGDDEKTKSKEEMVKGSSFCYGEKEKERFNRILGY 249
Query: 866 YVGTHNFHNFTTRIKAEDPSAVRFIISFNANSVVTVDGMDFVKCEVVGQSFMLHQIRKLV 1045
YVG+HNFHNFTTR KAEDPSA R+I+SF A + V V+G++FVKCEVVGQSFMLHQIRK++
Sbjct: 250 YVGSHNFHNFTTRTKAEDPSARRYIVSFEAKTTVNVEGIEFVKCEVVGQSFMLHQIRKMI 309
Query: 1046 GLAVAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFSSYNQKWKDTHEEISME 1225
G+AVAIMR+CAPESLI+TALQKDV INVPTAPEVGLYL+ECFF+SYNQKWKD+HEEISM+
Sbjct: 310 GVAVAIMRNCAPESLIQTALQKDVNINVPTAPEVGLYLDECFFTSYNQKWKDSHEEISMK 369
Query: 1226 AYVEQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRF 1360
Y E+AEDFKMK +YSHIA+ E KEGSVA+WLHSLN RNYPDLRF
Sbjct: 370 DYEEEAEDFKMKHIYSHIATTEHKEGSVALWLHSLNHRNYPDLRF 414
>ref|XP_002284797.2| PREDICTED: tRNA pseudouridine synthase A, mitochondrial-like [Vitis
vinifera]
Length = 503
Score = 578 bits (1489), Expect = e-162
Identities = 300/489 (61%), Positives = 353/489 (72%), Gaps = 57/489 (11%)
Frame = +2
Query: 119 STSDDEEQXXXXXXXNPDQKPRYKRRKIAIFLAYCGVGYQGMQKNPGAKTIEGELEEALY 298
+TSDDE+ +KPRYKRRKIAIF AYCGVGYQGMQKNPGAKTIEG+LEEAL+
Sbjct: 18 TTSDDED----CDTTTATKKPRYKRRKIAIFFAYCGVGYQGMQKNPGAKTIEGDLEEALF 73
Query: 299 LSGAIPEHDRGKPKRFDWARSARTDKGVSAVGQVVSGRFYVDPPGFIDRFNSNLPNQIRI 478
SGA+PE DRG PKRFDWARSARTDKGVSAVGQVVSGRFY+DPPG ++R NSNLP QIRI
Sbjct: 74 FSGAVPEQDRGHPKRFDWARSARTDKGVSAVGQVVSGRFYIDPPGLVERLNSNLPPQIRI 133
Query: 479 FGFNRVTSSFSAKKFCDRRRYVYLIPVFALDRNAHRDRESVLASLGSDNELVKCLECSER 658
FGF RVT+SF+AKKFCDRRRYVYLIPVFALD ++H DRESVLAS+GS+NELVKCLECSER
Sbjct: 134 FGFKRVTASFNAKKFCDRRRYVYLIPVFALDPSSHPDRESVLASVGSENELVKCLECSER 193
Query: 659 GRKVLGAMGKHYSGAKIXXXXXXXXXXXXXXXMASV-----------------ECGNG-- 781
GRKV+G MGK +K+ + + GN
Sbjct: 194 GRKVVGIMGKRSFESKVSVDDREDVEGESKNIVGAAILKSEAKEESMASSDNRHDGNSIS 253
Query: 782 -------------------DEKGVLENGSGFSYGEKEKERFNR----------------- 853
D + +N G S+ E+ + N+
Sbjct: 254 QPIKEGRCFQDNETSESTPDSRASAQNEPGVSHSEQSDKGENKLEKGSGFCYGDKERERF 313
Query: 854 --ILSHYVGTHNFHNFTTRIKAEDPSAVRFIISFNANSVVTVDGMDFVKCEVVGQSFMLH 1027
IL HYVGTHNFHNFTTRIKAEDPSA RFI+SF+ N++VTV+G++FVKCEVVGQSFMLH
Sbjct: 314 NRILGHYVGTHNFHNFTTRIKAEDPSAQRFIVSFDGNTIVTVEGIEFVKCEVVGQSFMLH 373
Query: 1028 QIRKLVGLAVAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFSSYNQKWKDTH 1207
QIRK++GLAVAIMR+ APESL+ETAL KDV INVPTAPEVGLYL+ECFFSSYN+KW+D+H
Sbjct: 374 QIRKMIGLAVAIMRNVAPESLLETALLKDVNINVPTAPEVGLYLDECFFSSYNKKWRDSH 433
Query: 1208 EEISMEAYVEQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRFVEDNETCTI 1387
EE+SM+AY E+AE+FKMK VYSHIA+ E KEG VA+WLHSLN RNYPDL + DN +
Sbjct: 434 EEVSMKAYAEEAEEFKMKHVYSHIAATEHKEGVVALWLHSLNHRNYPDL-CIGDNGAASD 492
Query: 1388 GENVQVESI 1414
G+N + E++
Sbjct: 493 GKNGEEETM 501
>emb|CBI18897.3| unnamed protein product [Vitis vinifera]
Length = 419
Score = 565 bits (1457), Expect = e-158
Identities = 284/420 (67%), Positives = 330/420 (78%), Gaps = 29/420 (6%)
Frame = +2
Query: 242 MQKNPGAKTIEGELEEALYLSGAIPEHDRGKPKRFDWARSARTDKGVSAVGQVVSGRFYV 421
MQKNPGAKTIEG+LEEAL+ SGA+PE DRG PKRFDWARSARTDKGVSAVGQVVSGRFY+
Sbjct: 1 MQKNPGAKTIEGDLEEALFFSGAVPEQDRGHPKRFDWARSARTDKGVSAVGQVVSGRFYI 60
Query: 422 DPPGFIDRFNSNLPNQIRIFGFNRVTSSFSAKKFCDRRRYVYLIPVFALDRNAHRDRESV 601
DPPG ++R NSNLP QIRIFGF RVT+SF+AKKFCDRRRYVYLIPVFALD ++H DRESV
Sbjct: 61 DPPGLVERLNSNLPPQIRIFGFKRVTASFNAKKFCDRRRYVYLIPVFALDPSSHPDRESV 120
Query: 602 LASLGSDNELVKCLECSERGRKVLGAMGKHYSGAKIXXXXXXXXXXXXXXXMASVE---- 769
LAS+GS+NELVKCLECSERGRKV+G MGK +K + V+
Sbjct: 121 LASVGSENELVKCLECSERGRKVVGIMGKRKGESKNIVQLGIPSNDEAAIVNSEVQEEIK 180
Query: 770 -------------------------CGNGDEKGVLENGSGFSYGEKEKERFNRILSHYVG 874
G+ K LE GSGF YG+KE+ERFNRIL HYVG
Sbjct: 181 ATSNNNNIDANNSISETIKEEAAKLSSEGENK--LEKGSGFCYGDKERERFNRILGHYVG 238
Query: 875 THNFHNFTTRIKAEDPSAVRFIISFNANSVVTVDGMDFVKCEVVGQSFMLHQIRKLVGLA 1054
THNFHNFTTRIKAEDPSA RFI+SF+ N++VTV+G++FVKCEVVGQSFMLHQIRK++GLA
Sbjct: 239 THNFHNFTTRIKAEDPSAQRFIVSFDGNTIVTVEGIEFVKCEVVGQSFMLHQIRKMIGLA 298
Query: 1055 VAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFSSYNQKWKDTHEEISMEAYV 1234
VAIMR+ APESL+ETAL KDV INVPTAPEVGLYL+ECFFSSYN+KW+D+HEE+SM+AY
Sbjct: 299 VAIMRNVAPESLLETALLKDVNINVPTAPEVGLYLDECFFSSYNKKWRDSHEEVSMKAYA 358
Query: 1235 EQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRFVEDNETCTIGENVQVESI 1414
E+AE+FKMK VYSHIA+ E KEG VA+WLHSLN RNYPDL + DN + G+N + E++
Sbjct: 359 EEAEEFKMKHVYSHIAATEHKEGVVALWLHSLNHRNYPDL-CIGDNGAASDGKNGEEETM 417
>ref|XP_003610360.1| tRNA pseudouridine synthase [Medicago truncatula]
gi|355511415|gb|AES92557.1| tRNA pseudouridine synthase
[Medicago truncatula] gi|388510220|gb|AFK43176.1| unknown
[Medicago truncatula]
Length = 530
Score = 565 bits (1455), Expect = e-158
Identities = 304/490 (62%), Positives = 344/490 (70%), Gaps = 60/490 (12%)
Frame = +2
Query: 119 STSDDEEQXXXXXXXNPDQKPRYKRRKIAIFLAYCGVGYQGMQKNPGAKTIEGELEEALY 298
+TSDDE K RYKRRK+AIF AYCGVGYQGMQKNPGAKTIEGELEEALY
Sbjct: 34 TTSDDE-----GCTTAEGSKVRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEGELEEALY 88
Query: 299 LSGAIPEHDRGKPKRFDWARSARTDKGVSAVGQVVSGRFYVDPPGFIDRFNSNLPNQIRI 478
+SGA+PE DRG KR+DWARSARTDKGVSAVGQVVSGRFY+DPPGF+DR NSNLP+QIRI
Sbjct: 89 VSGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLPSQIRI 148
Query: 479 FGFNRVTSSFSAKKFCDRRRYVYLIPVFALDRNAHRDRESVLASLGSDNELVKCLECSER 658
FGF RVT+SFSAKKFCDRRRYVYLIPVFALD HRDRE+VLASLGS+NELVKCL CSER
Sbjct: 149 FGFKRVTASFSAKKFCDRRRYVYLIPVFALDPCCHRDRETVLASLGSENELVKCLPCSER 208
Query: 659 GRKVLGAMGK-------------------------------HYSGAKIXXXXXXXXXXXX 745
GRKV G +G S +K
Sbjct: 209 GRKVEGVVGNSKRNLELEAVDVENGSSNKNDVVDSGVTKDVEVSLSKGDDNHLNKESIND 268
Query: 746 XXXMASVECGNG-------DEKGVLENGSGFSYGEKEKERFNR----------------- 853
SVE N DE + GS + G E+E+ NR
Sbjct: 269 NEGKVSVEEVNSKTVVSDQDEVAPINGGSENNLGILEEEKVNREDTAANGSGFCYGEKER 328
Query: 854 -----ILSHYVGTHNFHNFTTRIKAEDPSAVRFIISFNANSVVTVDGMDFVKCEVVGQSF 1018
IL YVGTHNFHNFTTRIK EDPSA RFIISF+A++ V V+GM+FVKCE+VGQSF
Sbjct: 329 ERFNNILKCYVGTHNFHNFTTRIKPEDPSAKRFIISFDASTTVVVEGMEFVKCEIVGQSF 388
Query: 1019 MLHQIRKLVGLAVAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFSSYNQKWK 1198
MLHQIRK++GLAVAIMR+CAPESLIE ALQKDV I VPTAPEVGLYL+ECFF+SYNQKWK
Sbjct: 389 MLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVSITVPTAPEVGLYLDECFFTSYNQKWK 448
Query: 1199 DTHEEISMEAYVEQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRFVEDNET 1378
DTHEE+SM+AY ++AEDFKM+ +Y HIAS E KEG+V +WLHSLN RNYPDLR + D E
Sbjct: 449 DTHEEVSMKAYEKEAEDFKMQYIYPHIASTEYKEGTVGLWLHSLNHRNYPDLRIL-DGEG 507
Query: 1379 CTIGENVQVE 1408
T + V+V+
Sbjct: 508 VTNDKKVEVD 517
>ref|XP_003610356.1| tRNA pseudouridine synthase [Medicago truncatula]
gi|355511411|gb|AES92553.1| tRNA pseudouridine synthase
[Medicago truncatula]
Length = 530
Score = 565 bits (1455), Expect = e-158
Identities = 304/490 (62%), Positives = 344/490 (70%), Gaps = 60/490 (12%)
Frame = +2
Query: 119 STSDDEEQXXXXXXXNPDQKPRYKRRKIAIFLAYCGVGYQGMQKNPGAKTIEGELEEALY 298
+TSDDE K RYKRRK+AIF AYCGVGYQGMQKNPGAKTIEGELEEALY
Sbjct: 34 TTSDDE-----GCTTAEGSKVRYKRRKVAIFFAYCGVGYQGMQKNPGAKTIEGELEEALY 88
Query: 299 LSGAIPEHDRGKPKRFDWARSARTDKGVSAVGQVVSGRFYVDPPGFIDRFNSNLPNQIRI 478
+SGA+PE DRG KR+DWARSARTDKGVSAVGQVVSGRFY+DPPGF+DR NSNLP+QIRI
Sbjct: 89 VSGAVPEQDRGLSKRYDWARSARTDKGVSAVGQVVSGRFYIDPPGFVDRLNSNLPSQIRI 148
Query: 479 FGFNRVTSSFSAKKFCDRRRYVYLIPVFALDRNAHRDRESVLASLGSDNELVKCLECSER 658
FGF RVT+SFSAKKFCDRRRYVYLIPVFALD HRDRE+VLASLGS+NELVKCL CSER
Sbjct: 149 FGFKRVTASFSAKKFCDRRRYVYLIPVFALDPCCHRDRETVLASLGSENELVKCLPCSER 208
Query: 659 GRKVLGAMGK-------------------------------HYSGAKIXXXXXXXXXXXX 745
GRKV G +G S +K
Sbjct: 209 GRKVEGVVGNSKRNLELEAVDVENGSSNKNDVVDSGVTKDVEVSLSKGDDNHLNKESIND 268
Query: 746 XXXMASVECGNG-------DEKGVLENGSGFSYGEKEKERFNR----------------- 853
SVE N DE + GS + G E+E+ NR
Sbjct: 269 NEGKVSVEEVNSKTVVSDEDEVAPINGGSENNLGILEEEKVNREDTAANGSGFCYGEKER 328
Query: 854 -----ILSHYVGTHNFHNFTTRIKAEDPSAVRFIISFNANSVVTVDGMDFVKCEVVGQSF 1018
IL YVGTHNFHNFTTRIK EDPSA RFIISF+A++ V V+GM+FVKCE+VGQSF
Sbjct: 329 ERFNNILKCYVGTHNFHNFTTRIKPEDPSAKRFIISFDASTTVVVEGMEFVKCEIVGQSF 388
Query: 1019 MLHQIRKLVGLAVAIMRDCAPESLIETALQKDVRINVPTAPEVGLYLEECFFSSYNQKWK 1198
MLHQIRK++GLAVAIMR+CAPESLIE ALQKDV I VPTAPEVGLYL+ECFF+SYNQKWK
Sbjct: 389 MLHQIRKMMGLAVAIMRNCAPESLIEKALQKDVSITVPTAPEVGLYLDECFFTSYNQKWK 448
Query: 1199 DTHEEISMEAYVEQAEDFKMKQVYSHIASMEQKEGSVAVWLHSLNLRNYPDLRFVEDNET 1378
DTHEE+SM+AY ++AEDFKM+ +Y HIAS E KEG+V +WLHSLN RNYPDLR + D E
Sbjct: 449 DTHEEVSMKAYEKEAEDFKMQYIYPHIASTEYKEGTVGLWLHSLNHRNYPDLRIL-DGEG 507
Query: 1379 CTIGENVQVE 1408
T + V+V+
Sbjct: 508 VTNDKKVEVD 517