BLASTX nr result
ID: Coptis24_contig00006782
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00006782
(1796 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera] 681 0.0
gb|AEK01241.1| ent-kaurene oxidase [Pyrus communis] 680 0.0
ref|XP_002282197.1| PREDICTED: ent-kaurene oxidase, chloroplasti... 680 0.0
gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima] 679 0.0
ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus commu... 672 0.0
>gb|AFD54196.1| ent-kaurene oxidase [Vitis vinifera]
Length = 508
Score = 681 bits (1758), Expect = 0.0
Identities = 324/460 (70%), Positives = 396/460 (86%)
Frame = +1
Query: 268 GLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTTDVAKEAMVTRFPSI 447
GLPLIGNLLQLK+KKPH+TF RWAE YGPI+SI+TGAST++VLN+++VAKEAMVTRF SI
Sbjct: 50 GLPLIGNLLQLKEKKPHKTFARWAETYGPIFSIRTGASTMIVLNSSEVAKEAMVTRFSSI 109
Query: 448 STRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQKRFRGHRDTMIKNMV 627
STRKLSNALKILT DKCMVATSDYN+FHK K +IL LGA AQKR R HRDT+I+N+
Sbjct: 110 STRKLSNALKILTFDKCMVATSDYNDFHKMVKGFILRNVLGAPAQKRHRCHRDTLIENIS 169
Query: 628 RTLHSYTKDKPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEELGTTLSREEIFEVL 807
+ LH++ K PL+ + +FE+E+FGLALKQ LGKD+ES +YVEELGTTLSREEIF VL
Sbjct: 170 KYLHAHVKTSPLEPVVLKKIFESEIFGLALKQALGKDIES-IYVEELGTTLSREEIFAVL 228
Query: 808 VLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKALVGQEKKRMASGEVL 987
V+DP+ GAI+VDWRDFFPYL W+PNKS++MKI+RM RR A+MKAL+G++KKR+ SGE
Sbjct: 229 VVDPMAGAIEVDWRDFFPYLSWIPNKSMEMKIQRMDFRRGALMKALIGEQKKRIGSGEEK 288
Query: 988 NSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELAKNKSHQDQLYQEIE 1167
NSY+DFL SE T +E+++ MLIWE IIE SDTTLVT+EWAMYELAK+ + Q+ LY+EI
Sbjct: 289 NSYIDFLLSEATTLTEKQIAMLIWETIIEISDTTLVTSEWAMYELAKDPNRQEILYREIH 348
Query: 1168 SVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTELGGYHIPAGSEIAV 1347
VCGS+K+TEEN+S+LPYL++VFHETLR++SP P++P+RYAHEDT+LGGYHIPAGS+IA+
Sbjct: 349 KVCGSNKLTEENLSKLPYLNSVFHETLRKYSPAPMVPVRYAHEDTQLGGYHIPAGSQIAI 408
Query: 1348 NIYGCNMDKHQWVEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVCAGSQQAMTISCIAI 1527
NIYGCNM+K QW PE+WKPERFLD KYD MD++KTMAFGGGKRVCAG+ QAM I+C +I
Sbjct: 409 NIYGCNMNKKQWENPEEWKPERFLDEKYDLMDLHKTMAFGGGKRVCAGALQAMLIACTSI 468
Query: 1528 ATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPMHAIITPRQ 1647
FVQEFEWKL GE+E+VDT+ LT+ KLHPM AII R+
Sbjct: 469 GRFVQEFEWKLMGGEEENVDTVALTSQKLHPMQAIIKARE 508
>gb|AEK01241.1| ent-kaurene oxidase [Pyrus communis]
Length = 514
Score = 680 bits (1755), Expect = 0.0
Identities = 314/459 (68%), Positives = 392/459 (85%)
Frame = +1
Query: 268 GLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTTDVAKEAMVTRFPSI 447
GLP++GNLLQLK+KKP++TFTRWAE YGPIYSI+TGA+T+VVLNT DVAKEAMVTR+ S+
Sbjct: 55 GLPVVGNLLQLKEKKPYKTFTRWAEEYGPIYSIRTGATTMVVLNTADVAKEAMVTRYSSM 114
Query: 448 STRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQKRFRGHRDTMIKNMV 627
STRKLS ALKILT DKCMVATSDYN+FH KRY+L+ LGANAQKR R RDTM +N+
Sbjct: 115 STRKLSKALKILTFDKCMVATSDYNDFHNMVKRYVLTNVLGANAQKRHRSQRDTMRENVA 174
Query: 628 RTLHSYTKDKPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEELGTTLSREEIFEVL 807
LH++ + P +A NFR +FE+ELFG+ALKQ +GKD+E + VEELGTTLSR++IF+VL
Sbjct: 175 SRLHAHVNNSPQEAINFREIFESELFGIALKQAIGKDIEDSINVEELGTTLSRDDIFKVL 234
Query: 808 VLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKALVGQEKKRMASGEVL 987
V D + GAI+VDWRDFFPYLRWVP K+++ ++R+ RR AVM L+ ++KKR+ASGE L
Sbjct: 235 VNDMMEGAIEVDWRDFFPYLRWVPIKTIENNMKRLSRRRIAVMNVLIDEQKKRIASGEEL 294
Query: 988 NSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELAKNKSHQDQLYQEIE 1167
+ ++D+L SE KT + E+++MLIWE IIET+DTTLVTTEW MYELAK++ QD+L++E++
Sbjct: 295 DCHIDYLLSEAKTLTPEQISMLIWETIIETADTTLVTTEWGMYELAKDQKRQDRLFEEVQ 354
Query: 1168 SVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTELGGYHIPAGSEIAV 1347
+VCG++KVTEE++SQ+PYL AVFHETLR+HSP PI+P+RY HEDTELGGY++P G+EIA+
Sbjct: 355 NVCGTNKVTEEHLSQMPYLGAVFHETLRKHSPAPIVPLRYVHEDTELGGYYVPVGTEIAI 414
Query: 1348 NIYGCNMDKHQWVEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVCAGSQQAMTISCIAI 1527
NIYGCNMDK+QW PE+WKPERFLD KYD MD+YKTMAFG GKRVCAGS QAM I+C I
Sbjct: 415 NIYGCNMDKNQWESPEEWKPERFLDPKYDPMDLYKTMAFGTGKRVCAGSLQAMLIACTTI 474
Query: 1528 ATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPMHAIITPR 1644
VQEFEWKL +GE+E+VDT+GLTTHKLHPMHAI+ PR
Sbjct: 475 GRLVQEFEWKLRDGEEENVDTVGLTTHKLHPMHAILKPR 513
>ref|XP_002282197.1| PREDICTED: ent-kaurene oxidase, chloroplastic [Vitis vinifera]
gi|302142385|emb|CBI19588.3| unnamed protein product
[Vitis vinifera]
Length = 508
Score = 680 bits (1754), Expect = 0.0
Identities = 324/460 (70%), Positives = 394/460 (85%)
Frame = +1
Query: 268 GLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTTDVAKEAMVTRFPSI 447
GLPLIGNLLQLK+KKPH+TF RWAE YGPI+SI+TGAST++VLN+++VAKEAMVTRF SI
Sbjct: 50 GLPLIGNLLQLKEKKPHKTFARWAETYGPIFSIRTGASTMIVLNSSEVAKEAMVTRFSSI 109
Query: 448 STRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQKRFRGHRDTMIKNMV 627
STRKLSNALKILT DKCMVATSDYN+FHK K +IL LGA AQKR R HRDT+I+N+
Sbjct: 110 STRKLSNALKILTFDKCMVATSDYNDFHKMVKGFILRNVLGAPAQKRHRCHRDTLIENIS 169
Query: 628 RTLHSYTKDKPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEELGTTLSREEIFEVL 807
+ LH++ K PL+ + +FE+E+FGLALKQ LGKD+ES +YVEELGTTLSREEIF VL
Sbjct: 170 KYLHAHVKTSPLEPVVLKKIFESEIFGLALKQALGKDIES-IYVEELGTTLSREEIFAVL 228
Query: 808 VLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKALVGQEKKRMASGEVL 987
V+DP+ GAI+VDWRDFFPYL W+PNKS++MKI+RM RR A+MKAL+G++KKR+ SGE
Sbjct: 229 VVDPMAGAIEVDWRDFFPYLSWIPNKSMEMKIQRMDFRRGALMKALIGEQKKRIGSGEEK 288
Query: 988 NSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELAKNKSHQDQLYQEIE 1167
N Y+DFL SE T +E+++ MLIWE IIE SDTTLVT+EWAMYELAK+ + Q+ LY+EI
Sbjct: 289 NCYIDFLLSEATTLTEKQIAMLIWETIIEISDTTLVTSEWAMYELAKDPNRQEILYREIR 348
Query: 1168 SVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTELGGYHIPAGSEIAV 1347
CGS+KVTEEN+S+LPYL++VFHETLR++SP PI+P+RYAHEDT+LGGYHIPAGS+IA+
Sbjct: 349 KFCGSNKVTEENLSKLPYLNSVFHETLRKYSPAPIVPVRYAHEDTQLGGYHIPAGSQIAI 408
Query: 1348 NIYGCNMDKHQWVEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVCAGSQQAMTISCIAI 1527
NIYGCNM+K QW PE+WKPERFLD KYD MD++KTMAFGGGKRVCAG+ QAM I+C +I
Sbjct: 409 NIYGCNMNKKQWENPEEWKPERFLDEKYDLMDLHKTMAFGGGKRVCAGALQAMLIACTSI 468
Query: 1528 ATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPMHAIITPRQ 1647
FVQEFEWKL GE+E+VDT+ LT+ KLHPM AII R+
Sbjct: 469 GRFVQEFEWKLMGGEEENVDTVALTSQKLHPMQAIIKARE 508
>gb|AEF32084.1| ent-kaurene oxidase [Castanea mollissima]
Length = 514
Score = 679 bits (1753), Expect = 0.0
Identities = 319/459 (69%), Positives = 402/459 (87%)
Frame = +1
Query: 268 GLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTTDVAKEAMVTRFPSI 447
GLP++GNLLQLK+KKP++TF RWAE++GPIYSI+TGAST+VV+N+T VAKEAMVTRF SI
Sbjct: 56 GLPVVGNLLQLKEKKPYKTFLRWAEIHGPIYSIRTGASTMVVVNSTHVAKEAMVTRFSSI 115
Query: 448 STRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQKRFRGHRDTMIKNMV 627
STRKLS AL++LTS+K MVATSDYNEFHK K+YIL+ LGANAQKR R HRDT+I+N++
Sbjct: 116 STRKLSKALELLTSNKSMVATSDYNEFHKMVKKYILAELLGANAQKRHRIHRDTLIENVL 175
Query: 628 RTLHSYTKDKPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEELGTTLSREEIFEVL 807
LH++TK+ PLQA NFR +FE+ELFGLA+KQ LG D++S ++VEELGTTLSREEI+ VL
Sbjct: 176 NKLHAHTKNSPLQAVNFRKIFESELFGLAMKQALGYDVDS-LFVEELGTTLSREEIYNVL 234
Query: 808 VLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKALVGQEKKRMASGEVL 987
V D L GAI+VDWRDFFPYL+W+PNKS +MKI+R+ +RR+AVM ++V ++KK +ASG+
Sbjct: 235 VSDMLKGAIEVDWRDFFPYLKWIPNKSFEMKIQRLASRRQAVMNSIVKEQKKSIASGKGE 294
Query: 988 NSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELAKNKSHQDQLYQEIE 1167
N Y+++L SE KT +E+++++L WE IIET+DTT+VTTEWAMYELAKN QD+LY EI+
Sbjct: 295 NCYLNYLLSEAKTLTEKQISILAWETIIETADTTVVTTEWAMYELAKNPKQQDRLYNEIQ 354
Query: 1168 SVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTELGGYHIPAGSEIAV 1347
+VCG+DK+TEE++S+LPYLSAVFHETLR++SP P++P+RYAHEDT+LGGY++PAG+EIAV
Sbjct: 355 NVCGTDKITEEHLSKLPYLSAVFHETLRKYSPSPLVPLRYAHEDTQLGGYYVPAGTEIAV 414
Query: 1348 NIYGCNMDKHQWVEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVCAGSQQAMTISCIAI 1527
NIYGCNMDK+QW PE+WKPERFLD KYD MDMYKTM+FG GKRVCAGS QA I+C +I
Sbjct: 415 NIYGCNMDKNQWETPEEWKPERFLDEKYDPMDMYKTMSFGSGKRVCAGSLQASLIACTSI 474
Query: 1528 ATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPMHAIITPR 1644
VQEFEW+L +GE E+VDTLGLTTHKL+PM AI+ PR
Sbjct: 475 GRLVQEFEWRLKDGEVENVDTLGLTTHKLYPMQAILQPR 513
>ref|XP_002510288.1| ent-kaurene oxidase, putative [Ricinus communis]
gi|223550989|gb|EEF52475.1| ent-kaurene oxidase, putative
[Ricinus communis]
Length = 513
Score = 672 bits (1734), Expect = 0.0
Identities = 318/460 (69%), Positives = 392/460 (85%)
Frame = +1
Query: 268 GLPLIGNLLQLKDKKPHQTFTRWAEVYGPIYSIKTGASTVVVLNTTDVAKEAMVTRFPSI 447
G P+IGNLLQLK+KKPH+TF RWAE YGPIYSI+TGAST++VLN+TDVAKEAMVTR+ SI
Sbjct: 55 GWPVIGNLLQLKEKKPHKTFMRWAETYGPIYSIRTGASTIIVLNSTDVAKEAMVTRYSSI 114
Query: 448 STRKLSNALKILTSDKCMVATSDYNEFHKTTKRYILSGFLGANAQKRFRGHRDTMIKNMV 627
STRKLS AL ILT DKCMVATSDY+EFHK KRY+L+ LGANAQ+R R HRDT+IKN+
Sbjct: 115 STRKLSKALVILTQDKCMVATSDYDEFHKMVKRYLLTNVLGANAQRRHRCHRDTLIKNIS 174
Query: 628 RTLHSYTKDKPLQAFNFRNVFENELFGLALKQTLGKDLESPVYVEELGTTLSREEIFEVL 807
H++ K P Q NFR++FE+ELFGL+LK+ LGKD++S +YV+ELGTTLSR+EIFEVL
Sbjct: 175 SRFHAHVKTNPEQTVNFRDIFESELFGLSLKEALGKDVQS-IYVDELGTTLSRKEIFEVL 233
Query: 808 VLDPLMGAIDVDWRDFFPYLRWVPNKSLQMKIERMFNRRKAVMKALVGQEKKRMASGEVL 987
VLDP+ GAIDVDWRDFFPYLRWVPNKS +MKI++M RRKAVM AL+ ++KKR+A GE +
Sbjct: 234 VLDPMEGAIDVDWRDFFPYLRWVPNKSWEMKIQQMHFRRKAVMGALIKEQKKRIALGEEV 293
Query: 988 NSYVDFLSSEGKTSSEEELNMLIWEPIIETSDTTLVTTEWAMYELAKNKSHQDQLYQEIE 1167
N Y+DFLSSE T ++E++ MLIWE I+ETSDTT+V TEWA+YELAKN + QD LY++I+
Sbjct: 294 NCYLDFLSSES-TLTDEQIRMLIWETIVETSDTTMVATEWAIYELAKNPNCQDLLYRQIQ 352
Query: 1168 SVCGSDKVTEENISQLPYLSAVFHETLRRHSPVPIIPMRYAHEDTELGGYHIPAGSEIAV 1347
+VCGS+K+TEE++S+LPYL+AVFHET+R++SP P+IP+RY HEDTELGGY+IPAGSEIA+
Sbjct: 353 NVCGSEKITEEHLSKLPYLNAVFHETIRKYSPAPVIPLRYVHEDTELGGYYIPAGSEIAI 412
Query: 1348 NIYGCNMDKHQWVEPEKWKPERFLDGKYDQMDMYKTMAFGGGKRVCAGSQQAMTISCIAI 1527
NIYGCNMDK++WV PE+W PERFL+G YD D++KTMAFG GKR CAG+ QA I+ +I
Sbjct: 413 NIYGCNMDKNRWVNPEEWNPERFLNGNYDSGDLHKTMAFGAGKRACAGALQASLIASTSI 472
Query: 1528 ATFVQEFEWKLSEGEKEDVDTLGLTTHKLHPMHAIITPRQ 1647
VQEFEW L GE+EDVD +GLTT KLHP+H I PR+
Sbjct: 473 GRLVQEFEWGLKAGEEEDVDIVGLTTRKLHPLHLSIKPRR 512