BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00005563
         (1651 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q42656.1|AGAL_COFAR RecName: Full=Alpha-galactosidase; AltNam...   566   e-164
gb|AEB98600.1| alpha-galactosidase [Nicotiana tabacum]                558   e-163
emb|CAI47559.1| alpha galactosidase [Coffea arabica]                  562   e-163
gb|AEB98601.1| alpha-galactosidase [Nicotiana tabacum]                558   e-162
ref|XP_002268711.1| PREDICTED: alpha-galactosidase [Vitis vinife...   554   e-162

>sp|Q42656.1|AGAL_COFAR RecName: Full=Alpha-galactosidase; AltName: Full=Alpha-D-galactoside
            galactohydrolase; AltName: Full=Melibiase; Flags:
            Precursor gi|504489|gb|AAA33022.1| alpha-galactosidase
            [Coffea arabica]
          Length = 378

 Score =  567 bits (1460), Expect(2) = e-164
 Identities = 270/341 (79%), Positives = 288/341 (84%)
 Frame = -3

Query: 1430 DIIRRNLMNNGLGRTPQMGWNSWNHFHCDIEEKLIWETADAMVSTGLAAVGYEYINLDDC 1251
            D  RR+L+ NGLG TP MGWNSWNHF C+++EKLI ETADAMVS GLAA+GY+YINLDDC
Sbjct: 9    DYTRRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDC 68

Query: 1250 WGELNRDSQGNLVPKASTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEV 1071
            W ELNRDSQGNLVPK STFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHE 
Sbjct: 69   WAELNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEE 128

Query: 1070 QDARTFASWEVDYLKYDNCHNTGTSPKERYPKMSQALLNSGRSIFFSLCEWGQEDPATWA 891
            QDA+TFASW VDYLKYDNC+N   SPKERYP MS+ALLNSGRSIFFSLCEWG+EDPATWA
Sbjct: 129  QDAKTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWA 188

Query: 890  PSLGNSWRTTGDIDDNWGSMTSLADQNDKWASYAGPGGWNDPDMLEVGNGGMTIEEYRSH 711
              +GNSWRTTGDIDD+W SMTS AD NDKWASYAGPGGWNDPDMLEVGNGGMT  EYRSH
Sbjct: 189  KEVGNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSH 248

Query: 710  FSIWALAKAPLLIGCDLRSMDKVTIELLSNKEVIAVNXXXXXXXXXXXXXXXXLEVWAGP 531
            FSIWALAKAPLLIGCD+RSMD  T +LLSN EVIAVN                LEVWAGP
Sbjct: 249  FSIWALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGP 308

Query: 530  LSGSRVTVVLWNRGSSQALINASWSDIGLKSSQVVQARNLW 408
            LSG RV V LWNRGSS A I A WSD+GL S+ VV AR+LW
Sbjct: 309  LSGKRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLW 349



 Score = 40.0 bits (92), Expect(2) = e-164
 Identities = 18/28 (64%), Positives = 22/28 (78%)
 Frame = -2

Query: 336 HSTQASVEGTISAVVDSHACAMYVLTPK 253
           HST+ SV+G ISA VD+H   MYVLTP+
Sbjct: 351 HSTEKSVKGQISAAVDAHDSKMYVLTPQ 378


>gb|AEB98600.1| alpha-galactosidase [Nicotiana tabacum]
          Length = 413

 Score =  558 bits (1439), Expect(2) = e-163
 Identities = 263/339 (77%), Positives = 291/339 (85%)
 Frame = -3

Query: 1424 IRRNLMNNGLGRTPQMGWNSWNHFHCDIEEKLIWETADAMVSTGLAAVGYEYINLDDCWG 1245
            IRR+L++NGLGRTPQMGW+SWNHF C+IEEK+I ETADAMVSTGLA++GYEY+N+DDCW 
Sbjct: 45   IRRSLLSNGLGRTPQMGWSSWNHFACNIEEKMIRETADAMVSTGLASLGYEYVNIDDCWA 104

Query: 1244 ELNRDSQGNLVPKASTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEVQD 1065
            ELNRDSQGN+VPK+STFPSGIKALADYVH KGLKLGIYSDAG+QTCSK MPGSLGHE QD
Sbjct: 105  ELNRDSQGNMVPKSSTFPSGIKALADYVHGKGLKLGIYSDAGSQTCSKQMPGSLGHEEQD 164

Query: 1064 ARTFASWEVDYLKYDNCHNTGTSPKERYPKMSQALLNSGRSIFFSLCEWGQEDPATWAPS 885
            A+TFASW VDYLKYDNC+N   SP+ERYP MS+AL NSGR+IF+SLCEWG +DPATWA S
Sbjct: 165  AKTFASWGVDYLKYDNCNNENRSPRERYPIMSKALQNSGRAIFYSLCEWGDDDPATWASS 224

Query: 884  LGNSWRTTGDIDDNWGSMTSLADQNDKWASYAGPGGWNDPDMLEVGNGGMTIEEYRSHFS 705
            +GNSWRTTGDI DNW SMTS AD NDKWASYAGPGGWNDPDMLEVGNGGMT  EYRSHFS
Sbjct: 225  VGNSWRTTGDISDNWDSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTAEYRSHFS 284

Query: 704  IWALAKAPLLIGCDLRSMDKVTIELLSNKEVIAVNXXXXXXXXXXXXXXXXLEVWAGPLS 525
            IWALAKAPL+IGCDLRSMD+   E+LSNKEVIAVN                LEVWAGPLS
Sbjct: 285  IWALAKAPLIIGCDLRSMDQTAHEILSNKEVIAVNQDKLGVQGKKVKQNGDLEVWAGPLS 344

Query: 524  GSRVTVVLWNRGSSQALINASWSDIGLKSSQVVQARNLW 408
            G R+ +VLWNR SS+A I A WSDIGL SS VV AR+LW
Sbjct: 345  GKRLAMVLWNRSSSKADITAYWSDIGLDSSTVVDARDLW 383



 Score = 44.7 bits (104), Expect(2) = e-163
 Identities = 19/28 (67%), Positives = 23/28 (82%)
 Frame = -2

Query: 336 HSTQASVEGTISAVVDSHACAMYVLTPK 253
           HST+ SV+G +SA +DSH C MYVLTPK
Sbjct: 385 HSTKGSVKGQLSASIDSHDCRMYVLTPK 412


>emb|CAI47559.1| alpha galactosidase [Coffea arabica]
          Length = 420

 Score =  562 bits (1448), Expect(2) = e-163
 Identities = 270/354 (76%), Positives = 291/354 (82%)
 Frame = -3

Query: 1469 ATAARLSNIGPKVDIIRRNLMNNGLGRTPQMGWNSWNHFHCDIEEKLIWETADAMVSTGL 1290
            A+A R+       D  RR+L+ NGLG TP MGWNSWNHF C+++EKLI ETADAM S GL
Sbjct: 38   ASARRMVKSPGTEDYTRRSLLANGLGLTPPMGWNSWNHFSCNLDEKLIRETADAMASKGL 97

Query: 1289 AAVGYEYINLDDCWGELNRDSQGNLVPKASTFPSGIKALADYVHSKGLKLGIYSDAGTQT 1110
            AA+GY+YINLDDCW ELNRDSQGNLVPK STFPSGIKALADYVHSKGLKLGIYSDAGTQT
Sbjct: 98   AALGYKYINLDDCWAELNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQT 157

Query: 1109 CSKTMPGSLGHEVQDARTFASWEVDYLKYDNCHNTGTSPKERYPKMSQALLNSGRSIFFS 930
            CSKTMPGSLGHE QDA+TFASW VDYLKYDNC++   SPKERYP MS+ALLNSGRSIFFS
Sbjct: 158  CSKTMPGSLGHEEQDAKTFASWGVDYLKYDNCNDNNISPKERYPIMSKALLNSGRSIFFS 217

Query: 929  LCEWGQEDPATWAPSLGNSWRTTGDIDDNWGSMTSLADQNDKWASYAGPGGWNDPDMLEV 750
            LCEWG EDPATWA  +GNSWRTTGDIDD+W SMTS AD NDKWASYAGPGGWNDPDMLEV
Sbjct: 218  LCEWGDEDPATWAKEVGNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEV 277

Query: 749  GNGGMTIEEYRSHFSIWALAKAPLLIGCDLRSMDKVTIELLSNKEVIAVNXXXXXXXXXX 570
            GNGGMT  EYRSHFSIWALAKAPLLIGCD+RS+D  T +LLSN EVIAVN          
Sbjct: 278  GNGGMTTTEYRSHFSIWALAKAPLLIGCDIRSIDGATFQLLSNAEVIAVNQDKLGVQGKK 337

Query: 569  XXXXXXLEVWAGPLSGSRVTVVLWNRGSSQALINASWSDIGLKSSQVVQARNLW 408
                  LEVWAGPLSG RV V LWNRGSS A I A WSD+GL S+ VV AR+LW
Sbjct: 338  VKTYGDLEVWAGPLSGKRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLW 391



 Score = 40.0 bits (92), Expect(2) = e-163
 Identities = 18/28 (64%), Positives = 22/28 (78%)
 Frame = -2

Query: 336 HSTQASVEGTISAVVDSHACAMYVLTPK 253
           HST+ SV+G ISA VD+H   MYVLTP+
Sbjct: 393 HSTEKSVKGQISAAVDAHDSKMYVLTPQ 420


>gb|AEB98601.1| alpha-galactosidase [Nicotiana tabacum]
          Length = 413

 Score =  558 bits (1439), Expect(2) = e-162
 Identities = 263/339 (77%), Positives = 291/339 (85%)
 Frame = -3

Query: 1424 IRRNLMNNGLGRTPQMGWNSWNHFHCDIEEKLIWETADAMVSTGLAAVGYEYINLDDCWG 1245
            IRR+L++NGLGRTPQMGW+SWNHF C+IEEK+I ETADAMVSTGLA++GYEY+N+DDCW 
Sbjct: 45   IRRSLLSNGLGRTPQMGWSSWNHFACNIEEKMIRETADAMVSTGLASLGYEYVNIDDCWA 104

Query: 1244 ELNRDSQGNLVPKASTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEVQD 1065
            ELNRDSQGN+VPK+STFPSGIKALADYVH KGLKLGIYSDAG+QTCSK MPGSLGHE QD
Sbjct: 105  ELNRDSQGNMVPKSSTFPSGIKALADYVHGKGLKLGIYSDAGSQTCSKQMPGSLGHEEQD 164

Query: 1064 ARTFASWEVDYLKYDNCHNTGTSPKERYPKMSQALLNSGRSIFFSLCEWGQEDPATWAPS 885
            A+TFASW VDYLKYDNC+N   SP+ERYP MS+AL NSGR+IF+SLCEWG +DPATWA S
Sbjct: 165  AKTFASWGVDYLKYDNCNNENRSPRERYPIMSKALQNSGRAIFYSLCEWGDDDPATWASS 224

Query: 884  LGNSWRTTGDIDDNWGSMTSLADQNDKWASYAGPGGWNDPDMLEVGNGGMTIEEYRSHFS 705
            +GNSWRTTGDI DNW SMTS AD NDKWASYAGPGGWNDPDMLEVGNGGMT  EYRSHFS
Sbjct: 225  VGNSWRTTGDISDNWDSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTAEYRSHFS 284

Query: 704  IWALAKAPLLIGCDLRSMDKVTIELLSNKEVIAVNXXXXXXXXXXXXXXXXLEVWAGPLS 525
            IWALAKAPL+IGCDLRSMD+   E+LSNKEVIAVN                LEVWAGPLS
Sbjct: 285  IWALAKAPLIIGCDLRSMDQTAHEILSNKEVIAVNQDKLGVQGKKVKQNGDLEVWAGPLS 344

Query: 524  GSRVTVVLWNRGSSQALINASWSDIGLKSSQVVQARNLW 408
            G R+ +VLWNR SS+A I A WSDIGL SS VV AR+LW
Sbjct: 345  GKRLAMVLWNRSSSKADITAYWSDIGLDSSTVVDARDLW 383



 Score = 42.7 bits (99), Expect(2) = e-162
 Identities = 18/27 (66%), Positives = 22/27 (81%)
 Frame = -2

Query: 336 HSTQASVEGTISAVVDSHACAMYVLTP 256
           HST+ SV+G +SA +DSH C MYVLTP
Sbjct: 385 HSTKGSVKGQLSASIDSHDCRMYVLTP 411


>ref|XP_002268711.1| PREDICTED: alpha-galactosidase [Vitis vinifera]
            gi|297742707|emb|CBI35341.3| unnamed protein product
            [Vitis vinifera]
          Length = 409

 Score =  554 bits (1427), Expect(2) = e-162
 Identities = 265/337 (78%), Positives = 285/337 (84%)
 Frame = -3

Query: 1418 RNLMNNGLGRTPQMGWNSWNHFHCDIEEKLIWETADAMVSTGLAAVGYEYINLDDCWGEL 1239
            RNL+ NGL  TPQMGWNSWNHF C I+E LI ETADAMVS+GLAA+GY Y+NLDDCWGE 
Sbjct: 44   RNLLANGLACTPQMGWNSWNHFGCKIDENLIKETADAMVSSGLAALGYHYVNLDDCWGEK 103

Query: 1238 NRDSQGNLVPKASTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEVQDAR 1059
            NRDS GNLV K STFPSGIKALADYVH KGLKLGIYSDAGTQTCSKTMPGSLGHE +DA+
Sbjct: 104  NRDSDGNLVAKHSTFPSGIKALADYVHKKGLKLGIYSDAGTQTCSKTMPGSLGHEEKDAK 163

Query: 1058 TFASWEVDYLKYDNCHNTGTSPKERYPKMSQALLNSGRSIFFSLCEWGQEDPATWAPSLG 879
            TFASWEVDYLKYDNC NTG  P+ERY KMS+ALLNSGRSIFFSLCEWGQEDPATWA  +G
Sbjct: 164  TFASWEVDYLKYDNCENTGIRPQERYIKMSKALLNSGRSIFFSLCEWGQEDPATWAKDVG 223

Query: 878  NSWRTTGDIDDNWGSMTSLADQNDKWASYAGPGGWNDPDMLEVGNGGMTIEEYRSHFSIW 699
            NSWRTTGDI+DNW SMTS AD+NDKWA++AGPGGWNDPDMLEVGNGGM  EEY+SHFSIW
Sbjct: 224  NSWRTTGDIEDNWDSMTSRADENDKWAAHAGPGGWNDPDMLEVGNGGMRKEEYQSHFSIW 283

Query: 698  ALAKAPLLIGCDLRSMDKVTIELLSNKEVIAVNXXXXXXXXXXXXXXXXLEVWAGPLSGS 519
            ALAKAPLLIGCD+RSMD  T ELLSNKEVIAVN                LEVWAGPLS +
Sbjct: 284  ALAKAPLLIGCDVRSMDNSTFELLSNKEVIAVNQDELGVQGRKVKKDGNLEVWAGPLSDN 343

Query: 518  RVTVVLWNRGSSQALINASWSDIGLKSSQVVQARNLW 408
            +V VVLWNRGSSQA + A WSDIGL  S VV AR+LW
Sbjct: 344  KVAVVLWNRGSSQANMTAHWSDIGLSPSAVVDARDLW 380



 Score = 46.6 bits (109), Expect(2) = e-162
 Identities = 20/28 (71%), Positives = 24/28 (85%)
 Frame = -2

Query: 336 HSTQASVEGTISAVVDSHACAMYVLTPK 253
           HSTQ+SV+G + A VDSHAC MY+LTPK
Sbjct: 382 HSTQSSVQGQLWAQVDSHACKMYILTPK 409