BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00005316
         (1283 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003523137.1| PREDICTED: 4-coumarate--CoA ligase-like 9-li...   569   e-160
ref|XP_003528115.1| PREDICTED: 4-coumarate--CoA ligase-like 9-li...   564   e-158
ref|XP_003602077.1| 4-coumarate-coa ligase [Medicago truncatula]...   553   e-155
ref|XP_002318708.1| acyl:coa ligase [Populus trichocarpa] gi|222...   549   e-154
ref|XP_002318707.1| 4-coumarate-coa ligase [Populus trichocarpa]...   549   e-154

>ref|XP_003523137.1| PREDICTED: 4-coumarate--CoA ligase-like 9-like [Glycine max]
          Length = 580

 Score =  569 bits (1467), Expect = e-160
 Identities = 274/360 (76%), Positives = 321/360 (89%)
 Frame = +3

Query: 3    QSDTAAILYSSGTTGRVKGVELTHRNFIALVSGFHAIRPVEENPPHPVAIFTVPLFHVFG 182
            QSD+AAIL+SSGTTGRVKGV LTHRNFI L+ GF+ +R V +  PHPV++FT+PLFHVFG
Sbjct: 221  QSDSAAILFSSGTTGRVKGVLLTHRNFITLIGGFYHLRNVADGDPHPVSLFTLPLFHVFG 280

Query: 183  FFMCVRAVALGETVVLMEKFDFVGMLKAVESYKVNYMPVSPPLVVAMAKLDVVDKFDLSS 362
            FFM VRA+A+GET+V M++FDF GMLKAVE Y + YMPVSPPLVVA+AK ++V K+DLSS
Sbjct: 281  FFMLVRAIAVGETLVFMQRFDFEGMLKAVERYGITYMPVSPPLVVALAKSELVKKYDLSS 340

Query: 363  LLTVGCGGAPLGKEVSKKFIARFPHVEIVQGYGMTETSGGATRMLGPDETKKHGSVGRLA 542
            L  +GCGGAPLGKEV+  F  +FP+VEI QGYG+TE+ GGA R+LGPDE+K+HGSVGRLA
Sbjct: 341  LRYLGCGGAPLGKEVADDFRGKFPNVEIGQGYGLTESGGGAARVLGPDESKRHGSVGRLA 400

Query: 543  ENMEAKIVHPDTGEALGPGQRGELWMRGPIIMKGYVGDDEATTSTMDSDGWLKTGDLCYI 722
            ENMEAKIV P TGEAL PGQ+GELW+RGP IMKGYVGD++AT  T+DS+GWLKTGDLCY 
Sbjct: 401  ENMEAKIVDPVTGEALPPGQKGELWLRGPTIMKGYVGDEKATAETLDSEGWLKTGDLCYF 460

Query: 723  DSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEIADVALIPYPDEEAGQIPMAFVV 902
            DSDGFLYIVDRLKELIKYKAYQVPPAELEH+L ++PEIAD A++PYPDEEAGQIPMAFVV
Sbjct: 461  DSDGFLYIVDRLKELIKYKAYQVPPAELEHILHTNPEIADAAVVPYPDEEAGQIPMAFVV 520

Query: 903  RKPGRNLSAAQVMEFVAKQVAPYKKIRRVAFISSIPKSPAGKILRRELIDRALSAPSARL 1082
            RKPG N++A QVMEFVAKQV+PYKKIRRV+FI SIPKSPAGKILRREL+D ALS+ S++L
Sbjct: 521  RKPGSNVTADQVMEFVAKQVSPYKKIRRVSFIKSIPKSPAGKILRRELVDYALSSGSSKL 580


>ref|XP_003528115.1| PREDICTED: 4-coumarate--CoA ligase-like 9-like [Glycine max]
          Length = 597

 Score =  564 bits (1453), Expect = e-158
 Identities = 271/360 (75%), Positives = 322/360 (89%)
 Frame = +3

Query: 3    QSDTAAILYSSGTTGRVKGVELTHRNFIALVSGFHAIRPVEENPPHPVAIFTVPLFHVFG 182
            QSD+AAIL+SSGTTGRVKGV LTHRNFIAL+ GF+ +R V ++ PHPV++FT+PLFHVFG
Sbjct: 238  QSDSAAILFSSGTTGRVKGVLLTHRNFIALIGGFYHLRMVVDDDPHPVSLFTLPLFHVFG 297

Query: 183  FFMCVRAVALGETVVLMEKFDFVGMLKAVESYKVNYMPVSPPLVVAMAKLDVVDKFDLSS 362
            FFM VRA+A+GET+V M +FDF GMLKAVE Y++ YMPVSPPLVVA+AK ++V K+D+SS
Sbjct: 298  FFMLVRAIAVGETLVFMHRFDFEGMLKAVERYRITYMPVSPPLVVALAKSELVKKYDMSS 357

Query: 363  LLTVGCGGAPLGKEVSKKFIARFPHVEIVQGYGMTETSGGATRMLGPDETKKHGSVGRLA 542
            L  +G GGAPLGKEV++ F A+FP+VEI QGYG+TE+ GGA R+LGPDE+K+HGSVGRL+
Sbjct: 358  LRYLGSGGAPLGKEVAEDFRAQFPNVEIGQGYGLTESGGGAARVLGPDESKRHGSVGRLS 417

Query: 543  ENMEAKIVHPDTGEALGPGQRGELWMRGPIIMKGYVGDDEATTSTMDSDGWLKTGDLCYI 722
            ENMEAKIV P TGEAL PGQ+GELW+RGP IMKGYVGD++AT  T+DS+GWLKTGDLCY 
Sbjct: 418  ENMEAKIVDPVTGEALSPGQKGELWLRGPTIMKGYVGDEKATAETLDSEGWLKTGDLCYF 477

Query: 723  DSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEIADVALIPYPDEEAGQIPMAFVV 902
            DSDGFLYIVDRLKELIKYKAYQVPPAELEH+L ++PEIAD A++PYPDEEAGQIP+AFVV
Sbjct: 478  DSDGFLYIVDRLKELIKYKAYQVPPAELEHILHTNPEIADAAVVPYPDEEAGQIPIAFVV 537

Query: 903  RKPGRNLSAAQVMEFVAKQVAPYKKIRRVAFISSIPKSPAGKILRRELIDRALSAPSARL 1082
            RK G N++A QVMEFVAKQV+PYKKIRRV+FI SIPKSPAGKILRREL+D ALS  S++L
Sbjct: 538  RKSGSNITADQVMEFVAKQVSPYKKIRRVSFIKSIPKSPAGKILRRELVDYALSCGSSKL 597


>ref|XP_003602077.1| 4-coumarate-coa ligase [Medicago truncatula]
            gi|355491125|gb|AES72328.1| 4-coumarate-coa ligase
            [Medicago truncatula]
          Length = 562

 Score =  553 bits (1426), Expect = e-155
 Identities = 267/364 (73%), Positives = 319/364 (87%), Gaps = 4/364 (1%)
 Frame = +3

Query: 3    QSDTAAILYSSGTTGRVKGVELTHRNFIALVSGF----HAIRPVEENPPHPVAIFTVPLF 170
            QSDTAAIL+SSGTTGRVKGV LTH NFIAL+ GF    HA     E+ PHPV++F +PLF
Sbjct: 199  QSDTAAILFSSGTTGRVKGVLLTHGNFIALIGGFCFMKHAYDDGIEDEPHPVSLFPLPLF 258

Query: 171  HVFGFFMCVRAVALGETVVLMEKFDFVGMLKAVESYKVNYMPVSPPLVVAMAKLDVVDKF 350
            HVFGFFM VRA+A+GET+VLM++FDF GMLKAVE Y++ YMPVSPPL+ A  K ++V K+
Sbjct: 259  HVFGFFMMVRALAMGETLVLMQRFDFEGMLKAVEKYRITYMPVSPPLITAFTKSELVKKY 318

Query: 351  DLSSLLTVGCGGAPLGKEVSKKFIARFPHVEIVQGYGMTETSGGATRMLGPDETKKHGSV 530
            D+SS+  +G GGAPLGKEV++ F A+FP+VEIVQGYG+TE+ GGA RM+G DE K+HGSV
Sbjct: 319  DISSIRLLGSGGAPLGKEVAESFKAKFPNVEIVQGYGLTESGGGAARMIGFDEAKRHGSV 378

Query: 531  GRLAENMEAKIVHPDTGEALGPGQRGELWMRGPIIMKGYVGDDEATTSTMDSDGWLKTGD 710
            GRLAENMEAKIV P TGEAL PGQ+GELW+RGP IMKGYVGDD+AT  T+DS+GWLKTGD
Sbjct: 379  GRLAENMEAKIVDPVTGEALSPGQKGELWLRGPTIMKGYVGDDKATVETLDSEGWLKTGD 438

Query: 711  LCYIDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEIADVALIPYPDEEAGQIPM 890
            LCY DSDG+L+IVDRLKELIKYKAYQVPPAELEH+L ++PEIAD A++PYPDE+AGQIPM
Sbjct: 439  LCYFDSDGYLFIVDRLKELIKYKAYQVPPAELEHILHTNPEIADAAVVPYPDEDAGQIPM 498

Query: 891  AFVVRKPGRNLSAAQVMEFVAKQVAPYKKIRRVAFISSIPKSPAGKILRRELIDRALSAP 1070
            AFVVRKPG N++AAQVM++VAKQV PYKKIRRV+FI+SIPKSPAGKILRREL+D AL++ 
Sbjct: 499  AFVVRKPGSNITAAQVMDYVAKQVTPYKKIRRVSFINSIPKSPAGKILRRELVDIALTSG 558

Query: 1071 SARL 1082
            S++L
Sbjct: 559  SSKL 562


>ref|XP_002318708.1| acyl:coa ligase [Populus trichocarpa] gi|222859381|gb|EEE96928.1|
            acyl:coa ligase [Populus trichocarpa]
          Length = 550

 Score =  549 bits (1414), Expect = e-154
 Identities = 267/363 (73%), Positives = 314/363 (86%), Gaps = 3/363 (0%)
 Frame = +3

Query: 3    QSDTAAILYSSGTTGRVKGVELTHRNFIALVSGFHA-IRPVEENPPH--PVAIFTVPLFH 173
            QSD AAILYSSGTTGRVKGV LTHRN IAL+SGFH  ++  E N P   PV++F +PLFH
Sbjct: 188  QSDIAAILYSSGTTGRVKGVALTHRNLIALISGFHHNMKEPEPNQPEQPPVSLFILPLFH 247

Query: 174  VFGFFMCVRAVALGETVVLMEKFDFVGMLKAVESYKVNYMPVSPPLVVAMAKLDVVDKFD 353
            VFGFFM + A + GET+VLME+FDFV MLK VE Y+V YMPVSPPL+VA  K D+ +K+D
Sbjct: 248  VFGFFMSINAFSRGETLVLMERFDFVQMLKHVEKYRVTYMPVSPPLIVAFVKSDLTEKYD 307

Query: 354  LSSLLTVGCGGAPLGKEVSKKFIARFPHVEIVQGYGMTETSGGATRMLGPDETKKHGSVG 533
            LSSL ++GCGGAPLGKEV+ KF  +FPHVEIVQGYG+TET GGA+R LGP+ET +H SVG
Sbjct: 308  LSSLRSLGCGGAPLGKEVADKFKEKFPHVEIVQGYGLTETGGGASRTLGPEETSQHASVG 367

Query: 534  RLAENMEAKIVHPDTGEALGPGQRGELWMRGPIIMKGYVGDDEATTSTMDSDGWLKTGDL 713
            RL+ENMEAKIV P+TGE+LGPGQRGELW+RGP +MKGYVGD++AT  T+  +GWLKTGDL
Sbjct: 368  RLSENMEAKIVDPETGESLGPGQRGELWLRGPTVMKGYVGDEKATAETLHPEGWLKTGDL 427

Query: 714  CYIDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEIADVALIPYPDEEAGQIPMA 893
            CY DSDGFLYIVDRLKELIKYKA+QVPPAELE LLQS+PEIAD A+IPYPDEEAG+IPMA
Sbjct: 428  CYFDSDGFLYIVDRLKELIKYKAFQVPPAELEKLLQSNPEIADAAVIPYPDEEAGEIPMA 487

Query: 894  FVVRKPGRNLSAAQVMEFVAKQVAPYKKIRRVAFISSIPKSPAGKILRRELIDRALSAPS 1073
            +VVRKPG N++ AQ+M+ +AKQVAPYKKIRRV FIS+IPKSPAGKILRREL++ ALS  S
Sbjct: 488  YVVRKPGSNITEAQIMDSIAKQVAPYKKIRRVDFISAIPKSPAGKILRRELVNHALSGAS 547

Query: 1074 ARL 1082
            ++L
Sbjct: 548  SKL 550


>ref|XP_002318707.1| 4-coumarate-coa ligase [Populus trichocarpa]
            gi|222859380|gb|EEE96927.1| 4-coumarate-coa ligase
            [Populus trichocarpa]
          Length = 548

 Score =  549 bits (1414), Expect = e-154
 Identities = 267/363 (73%), Positives = 314/363 (86%), Gaps = 3/363 (0%)
 Frame = +3

Query: 3    QSDTAAILYSSGTTGRVKGVELTHRNFIALVSGFHA-IRPVEENPPH--PVAIFTVPLFH 173
            QSD AAILYSSGTTGRVKGV LTHRN IAL+SGFH  ++  E N P   PV++F +PLFH
Sbjct: 186  QSDIAAILYSSGTTGRVKGVALTHRNLIALISGFHHNMKEPEPNQPEQPPVSLFILPLFH 245

Query: 174  VFGFFMCVRAVALGETVVLMEKFDFVGMLKAVESYKVNYMPVSPPLVVAMAKLDVVDKFD 353
            VFGFFM + A + GET+VLME+FDFV MLK VE Y+V YMPVSPPL+VA  K D+ +K+D
Sbjct: 246  VFGFFMSINAFSRGETLVLMERFDFVQMLKHVEKYRVTYMPVSPPLIVAFVKSDLTEKYD 305

Query: 354  LSSLLTVGCGGAPLGKEVSKKFIARFPHVEIVQGYGMTETSGGATRMLGPDETKKHGSVG 533
            LSSL ++GCGGAPLGKEV+ KF  +FPHVEIVQGYG+TET GGA+R LGP+ET +H SVG
Sbjct: 306  LSSLRSLGCGGAPLGKEVADKFKEKFPHVEIVQGYGLTETGGGASRTLGPEETSQHASVG 365

Query: 534  RLAENMEAKIVHPDTGEALGPGQRGELWMRGPIIMKGYVGDDEATTSTMDSDGWLKTGDL 713
            RL+ENMEAKIV P+TGE+LGPGQRGELW+RGP +MKGYVGD++AT  T+  +GWLKTGDL
Sbjct: 366  RLSENMEAKIVDPETGESLGPGQRGELWLRGPTVMKGYVGDEKATAETLHPEGWLKTGDL 425

Query: 714  CYIDSDGFLYIVDRLKELIKYKAYQVPPAELEHLLQSHPEIADVALIPYPDEEAGQIPMA 893
            CY DSDGFLYIVDRLKELIKYKA+QVPPAELE LLQS+PEIAD A+IPYPDEEAG+IPMA
Sbjct: 426  CYFDSDGFLYIVDRLKELIKYKAFQVPPAELEKLLQSNPEIADAAVIPYPDEEAGEIPMA 485

Query: 894  FVVRKPGRNLSAAQVMEFVAKQVAPYKKIRRVAFISSIPKSPAGKILRRELIDRALSAPS 1073
            +VVRKPG N++ AQ+M+ +AKQVAPYKKIRRV FIS+IPKSPAGKILRREL++ ALS  S
Sbjct: 486  YVVRKPGSNITEAQIMDSIAKQVAPYKKIRRVDFISAIPKSPAGKILRRELVNHALSGAS 545

Query: 1074 ARL 1082
            ++L
Sbjct: 546  SKL 548