BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00004497
         (1392 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]       605   0.0  
sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; Alt...   473   e-148
dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]       471   e-145
gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. g...   464   e-145
gb|ACO90219.1| stylopine synthase [Eschscholzia californica]          387   e-119

>dbj|BAF98470.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 491

 Score =  605 bits (1560), Expect(2) = 0.0
 Identities = 312/354 (88%), Positives = 318/354 (89%)
 Frame = -1

Query: 1254 MEGSFLWLIGSXXXXXXXXXXXVRMXXXXXXXXXKWPSGPKRLPIIGNXXXXXXXXXHVT 1075
            MEGSFLWLIGS           VRM         KWPSGPKRLPIIGN         HVT
Sbjct: 1    MEGSFLWLIGSTVAIVLVITTIVRMSSSSSSSKKKWPSGPKRLPIIGNLHLLGGDLLHVT 60

Query: 1074 LAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVLVSKSAEFGQRDTPEIFKIFTVGQNN 895
            LAKLAKVHGSVMTIWIG WRPIIVISDINSAWEVLVSKSAE+GQRDTP+IFK  TVGQNN
Sbjct: 61   LAKLAKVHGSVMTIWIGRWRPIIVISDINSAWEVLVSKSAEYGQRDTPDIFKTLTVGQNN 120

Query: 894  IAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFREEDMKRLIHEMSREALNKNGTIKPLD 715
            I+MSDIGPFWHNVRK LQNGALSPLNVAAQTQFREED+KRLIHEMSREALNKNG IKPLD
Sbjct: 121  ISMSDIGPFWHNVRKGLQNGALSPLNVAAQTQFREEDVKRLIHEMSREALNKNGIIKPLD 180

Query: 714  HMKKNTARLLGRLIFGPTFDNDKFVESMLYEIEDVLRLSGYARLTEAFYYAKYLPSQKKV 535
            HMKKNTARLLGRLIFGPTFDNDKFVES+LYEIEDVLRLSGYARLTEAFYYAKYLPSQKKV
Sbjct: 181  HMKKNTARLLGRLIFGPTFDNDKFVESVLYEIEDVLRLSGYARLTEAFYYAKYLPSQKKV 240

Query: 534  AREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFFLGVDSTSST 355
            AREAYLAKYRIEEL+RPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELF LGVDSTSST
Sbjct: 241  AREAYLAKYRIEELMRPLLSSNPPTNSYLYFLQSQNYSKEIIIFCTFELFLLGVDSTSST 300

Query: 354  ATWALAYLIHEQAVQEKLYEDIRMTIGDVDLVKIEDLSKLKYLQAVVKETMRMK 193
            ATWALAYLIHEQAVQEKL EDIRMTIGDVDLVKIEDLSKLKYLQAVVKETMRMK
Sbjct: 301  ATWALAYLIHEQAVQEKLNEDIRMTIGDVDLVKIEDLSKLKYLQAVVKETMRMK 354



 Score = 85.5 bits (210), Expect(2) = 0.0
 Identities = 41/41 (100%), Positives = 41/41 (100%)
 Frame = -2

Query: 125 TMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALH 3
           TMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALH
Sbjct: 350 TMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALH 390


>sp|Q948Y1.1|C719A_COPJA RecName: Full=(S)-canadine synthase; AltName:
            Full=(S)-tetrahydrocolumbamine oxidase
            (methylenedioxy-bridge-forming); AltName: Full=Cytochrome
            P450 719A1 gi|15822820|dbj|BAB68769.1| methylenedioxy
            bridge-forming enzyme [Coptis japonica]
          Length = 491

 Score =  473 bits (1217), Expect(2) = e-148
 Identities = 233/319 (73%), Positives = 264/319 (82%)
 Frame = -1

Query: 1149 WPSGPKRLPIIGNXXXXXXXXXHVTLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVL 970
            WPSGP++LPIIGN         HV LAKLAKVHGSVMTIWIGSWRP+IVISDI  AWEVL
Sbjct: 36   WPSGPRKLPIIGNLHQLGDDVLHVALAKLAKVHGSVMTIWIGSWRPVIVISDIEKAWEVL 95

Query: 969  VSKSAEFGQRDTPEIFKIFTVGQNNIAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFRE 790
            V+KSA++G RD PEI KI +   + I+ SD G FW NVRK LQ+GA+ PLNVAAQ Q++E
Sbjct: 96   VNKSADYGARDMPEITKIASASWHTISTSDAGSFWQNVRKGLQSGAMGPLNVAAQNQYQE 155

Query: 789  EDMKRLIHEMSREALNKNGTIKPLDHMKKNTARLLGRLIFGPTFDNDKFVESMLYEIEDV 610
             DMKRLI  MS EA N NG +KPLDH+KKNT RLL RLIFG  FD++KF+ESM YEIED+
Sbjct: 156  RDMKRLIKAMSDEAANNNGIVKPLDHIKKNTVRLLTRLIFGQAFDDNKFIESMHYEIEDI 215

Query: 609  LRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQ 430
            +R+SGYARL EAFYYAKYLPS KK  REA+L K R+EELVRPLLSS PPTNSYLYFL SQ
Sbjct: 216  IRISGYARLAEAFYYAKYLPSHKKAEREAFLVKCRVEELVRPLLSSKPPTNSYLYFLLSQ 275

Query: 429  NYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHEQAVQEKLYEDIRMTIGDVDLVKIE 250
            N+ +E+IIFC FEL+ LGVDSTSST TWALAYLI EQ  QEKLY+DIRMT+GDVDLVKIE
Sbjct: 276  NFEEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQGAQEKLYQDIRMTLGDVDLVKIE 335

Query: 249  DLSKLKYLQAVVKETMRMK 193
            D++KLKYLQ VVKETMRMK
Sbjct: 336  DVNKLKYLQGVVKETMRMK 354



 Score = 79.0 bits (193), Expect(2) = e-148
 Identities = 37/41 (90%), Positives = 40/41 (97%)
 Frame = -2

Query: 125 TMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALH 3
           TMRMKPIAPLAIPHKTA +TTLMGTKVA+GT+IMVNLYALH
Sbjct: 350 TMRMKPIAPLAIPHKTAKETTLMGTKVAKGTRIMVNLYALH 390


>dbj|BAF98471.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 508

 Score =  471 bits (1211), Expect(2) = e-145
 Identities = 244/369 (66%), Positives = 286/369 (77%), Gaps = 5/369 (1%)
 Frame = -1

Query: 1284 LHSIVTISTEMEGSFLWLIGSXXXXXXXXXXXVRMXXXXXXXXXK--WPSGPKRLPIIGN 1111
            L ++V +  EMEGS L L G+           VR+         +  WPSGPK+LPIIGN
Sbjct: 3    LRNMVAMVAEMEGSLL-LAGATLAILLTISTIVRITSSPSSSFSQMTWPSGPKKLPIIGN 61

Query: 1110 XXXXXXXXXHVTLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVLVSKSAEFGQRDTP 931
                     HVTLAKLAKVHG VMTIW+GSW PIIVISDINSAWEVLV+KSA++G R+  
Sbjct: 62   LHQLRGELLHVTLAKLAKVHGGVMTIWMGSWVPIIVISDINSAWEVLVNKSADYGAREVT 121

Query: 930  ---EIFKIFTVGQNNIAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFREEDMKRLIHEM 760
               E+ +   +  +NI+ SD+GP WHN+RK LQ+GALSPL+VA+QTQ RE DMKR++HEM
Sbjct: 122  YLHELLRDSAMHPHNISSSDVGPLWHNIRKGLQSGALSPLSVASQTQHRERDMKRVVHEM 181

Query: 759  SREALNKNGTIKPLDHMKKNTARLLGRLIFGPTFDNDKFVESMLYEIEDVLRLSGYARLT 580
            SRE +N NGTIKPL H+K +TARLLGRLIFGP+F++ KF+ESMLYEIED +R S Y  LT
Sbjct: 182  SREIVNNNGTIKPLVHIKNSTARLLGRLIFGPSFNDSKFIESMLYEIEDFIRTSSYTSLT 241

Query: 579  EAFYYAKYLPSQKKVAREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQNYSKEIIIFC 400
             +FYYAKYLPSQ+K  REA L KYRIEELVRPLLSS PPTN+YLYFL SQN S+E+ IFC
Sbjct: 242  NSFYYAKYLPSQRKATREAKLGKYRIEELVRPLLSSKPPTNAYLYFLLSQNLSEEVTIFC 301

Query: 399  TFELFFLGVDSTSSTATWALAYLIHEQAVQEKLYEDIRMTIGDVDLVKIEDLSKLKYLQA 220
             FELF LGVDS SST TWALAY+I EQ  QEKLY+DIRMTIGDVDLVKIED++KLKYLQ+
Sbjct: 302  IFELFLLGVDSASSTTTWALAYMIREQEAQEKLYQDIRMTIGDVDLVKIEDVNKLKYLQS 361

Query: 219  VVKETMRMK 193
            VVKETMRMK
Sbjct: 362  VVKETMRMK 370



 Score = 72.0 bits (175), Expect(2) = e-145
 Identities = 31/41 (75%), Positives = 36/41 (87%)
 Frame = -2

Query: 125 TMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALH 3
           TMRMKPIAPLA+PH    DTTLMGTK+ +GT++MVNLYALH
Sbjct: 366 TMRMKPIAPLALPHNAGKDTTLMGTKIVKGTRVMVNLYALH 406


>gb|AAU20771.1| (S)-canadine synthase [Thalictrum flavum subsp. glaucum]
          Length = 492

 Score =  464 bits (1193), Expect(2) = e-145
 Identities = 227/319 (71%), Positives = 264/319 (82%)
 Frame = -1

Query: 1149 WPSGPKRLPIIGNXXXXXXXXXHVTLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVL 970
            WP+GP +LPIIGN         HV LAKLA+VHGSVMT+WIG+WRPIIVISDI+ AWEVL
Sbjct: 37   WPTGPNKLPIIGNLHQLGGDVLHVALAKLAQVHGSVMTVWIGNWRPIIVISDIDKAWEVL 96

Query: 969  VSKSAEFGQRDTPEIFKIFTVGQNNIAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFRE 790
            V+KS+++G RD PEI KI +   + I+ SD G FW  VRK LQ+GA+ PLN+AAQ+QF+E
Sbjct: 97   VNKSSDYGARDMPEITKIASASWHTISTSDAGAFWQTVRKGLQSGAMGPLNIAAQSQFQE 156

Query: 789  EDMKRLIHEMSREALNKNGTIKPLDHMKKNTARLLGRLIFGPTFDNDKFVESMLYEIEDV 610
             DMKRLI  +  EA   N  +KPLDH+KKNT RLL RLIFG TFD+DKFVESM +EI+D+
Sbjct: 157  RDMKRLIQALRDEAAQNNNIVKPLDHIKKNTVRLLTRLIFGQTFDDDKFVESMHFEIDDI 216

Query: 609  LRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQ 430
            +R+SGYARL EAFYYAKYLPS KK  REAYL K R+E+LV PLLSSNPPTNSYLYFL SQ
Sbjct: 217  IRISGYARLAEAFYYAKYLPSHKKAEREAYLVKCRVEDLVSPLLSSNPPTNSYLYFLLSQ 276

Query: 429  NYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHEQAVQEKLYEDIRMTIGDVDLVKIE 250
             +S+E+IIFC FEL+ LGVDSTSST TWALAYLI EQA+QEKLY+D RMT+GDVDLVKIE
Sbjct: 277  RFSEEVIIFCIFELYLLGVDSTSSTTTWALAYLIREQAIQEKLYQDTRMTLGDVDLVKIE 336

Query: 249  DLSKLKYLQAVVKETMRMK 193
            D+SKLKYLQAVVKETMRMK
Sbjct: 337  DVSKLKYLQAVVKETMRMK 355



 Score = 79.0 bits (193), Expect(2) = e-145
 Identities = 37/41 (90%), Positives = 40/41 (97%)
 Frame = -2

Query: 125 TMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALH 3
           TMRMKPIAPLAIPHKTA DTTLMG+KVA+GT+IMVNLYALH
Sbjct: 351 TMRMKPIAPLAIPHKTAKDTTLMGSKVAKGTRIMVNLYALH 391


>gb|ACO90219.1| stylopine synthase [Eschscholzia californica]
          Length = 495

 Score =  387 bits (994), Expect(2) = e-119
 Identities = 190/321 (59%), Positives = 237/321 (73%), Gaps = 2/321 (0%)
 Frame = -1

Query: 1149 WPSGPKRLPIIGNXXXXXXXXXHVTLAKLAKVHGSVMTIWIGSWRPIIVISDINSAWEVL 970
            WP+GPK LPIIGN          V L  LAKVHGSVMTIWIGSWRP+IV+SDI  AWEVL
Sbjct: 39   WPAGPKTLPIIGNMHLLGGTALQVVLHNLAKVHGSVMTIWIGSWRPVIVVSDIERAWEVL 98

Query: 969  VSKSAEFGQRDTPEIFKIFTVGQNNIAMSDIGPFWHNVRKVLQNGALSPLNVAAQTQFRE 790
            V+KS+++  RD P+I KI +     I+ SD GP W N+RK LQN ALSP N+AAQ QF+E
Sbjct: 99   VNKSSDYSARDMPDITKIISADWKTISTSDSGPHWTNLRKGLQNVALSPHNLAAQFQFQE 158

Query: 789  EDMKRLIHEMSREALNKNGTIKPLDHMKKNTARLLGRLIFGPTFDNDKFVESMLYEIEDV 610
            +DM ++I  +  EA N NG +KPLDHMKK T RL+ RL+FG  F+NDK+V+ M   IE++
Sbjct: 159  KDMTKMIQTLQEEARNNNGIVKPLDHMKKATLRLISRLVFGQDFNNDKYVDDMHLAIEEL 218

Query: 609  LRLSGYARLTEAFYYAKYLPSQKKVAREAYLAKYRIEELVRPLLSSNPPTNSYLYFLQSQ 430
            +R+SGYARL EAFYYAKYLPS KK  RE   A+ R++ LV P LS NPPTN+YL+FL+SQ
Sbjct: 219  IRVSGYARLAEAFYYAKYLPSHKKAVREVEEAQRRVQNLVSPFLSLNPPTNTYLHFLRSQ 278

Query: 429  NYSKEIIIFCTFELFFLGVDSTSSTATWALAYLIHEQAVQEKLYEDIRMTIGDVD--LVK 256
             Y  E+IIF  FE + LGVDSTS T  WALA+LI E  VQEKLY+++      +D  ++K
Sbjct: 279  KYDDEVIIFAIFEAYLLGVDSTSLTTAWALAFLIRESNVQEKLYQELESFASKMDRKILK 338

Query: 255  IEDLSKLKYLQAVVKETMRMK 193
            +ED++KL+YLQAV+KETMRMK
Sbjct: 339  VEDINKLQYLQAVIKETMRMK 359



 Score = 69.3 bits (168), Expect(2) = e-119
 Identities = 30/41 (73%), Positives = 36/41 (87%)
 Frame = -2

Query: 125 TMRMKPIAPLAIPHKTANDTTLMGTKVARGTQIMVNLYALH 3
           TMRMKPIAPLAIPHK   DT+LMG K+ +GT++MVN+YALH
Sbjct: 355 TMRMKPIAPLAIPHKACRDTSLMGKKIDKGTRVMVNIYALH 395