BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Coptis24_contig00004442
         (1728 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]       912   0.0  
gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]                   677   0.0  
ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [V...   604   e-170
ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]...   603   e-170
emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera]   603   e-170

>dbj|BAF98467.1| cytochrome P450 [Coptis japonica var. dissecta]
          Length = 503

 Score =  912 bits (2358), Expect = 0.0
 Identities = 454/487 (93%), Positives = 463/487 (95%)
 Frame = -3

Query: 1726 IKFLVPKRYKNXXXXXXXXXXXXXXXLFKKPLHRTLAKLSDKYGHVLYLQFGARPVLVVS 1547
            IKFLVPKRYKN               LFKKPLHRTLAKLSDKYGHVLYLQFG+RPVLVVS
Sbjct: 17   IKFLVPKRYKNLPPGPPSLPLIGHLHLFKKPLHRTLAKLSDKYGHVLYLQFGSRPVLVVS 76

Query: 1546 SPSAVEDCLAKNDIIFANRPHLLMGKILGHDYTTLLWAPYGPNWRNLRKISSIELLSANR 1367
            SPSA EDCLAKNDIIFANRPHLLMGKILGHDYTTLLWAPYGPNWRNLRKIS+IELLSANR
Sbjct: 77   SPSAAEDCLAKNDIIFANRPHLLMGKILGHDYTTLLWAPYGPNWRNLRKISAIELLSANR 136

Query: 1366 LQSYSDIRHAEVKSMVQRLSGQGCDYRTVEMKTMIFELTLNVMMTMIAGKRYFGGQVEEL 1187
            LQSYSDIRH EVKSMVQRLSGQG +YRTVEMKTMIFELTLNVMMTMIAGKRY+GGQVEEL
Sbjct: 137  LQSYSDIRHDEVKSMVQRLSGQGNEYRTVEMKTMIFELTLNVMMTMIAGKRYYGGQVEEL 196

Query: 1186 EQAKRFQEIVQGTLSISGASNIGDFLPILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHR 1007
            EQAKRFQEIVQGTL+ISGASNIGDFLPILSWIG+KGIEKKVWNLKEKRDKFLQELIEEHR
Sbjct: 197  EQAKRFQEIVQGTLAISGASNIGDFLPILSWIGIKGIEKKVWNLKEKRDKFLQELIEEHR 256

Query: 1006 ISRNGGTQNKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMS 827
            ISR+GG Q KEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMS
Sbjct: 257  ISRSGGMQTKEKNATLIDVLLSLQETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMS 316

Query: 826  LLLNNPQVIKKAQAEIDNTLEQGRLINESDVNKLPYLHSIINETLRIYPAGPLLVPHESS 647
            LLLNNPQVIKKAQAEIDN LEQGRLINESDVNKLPYLHSII ETLRIYPAGPLLVPHESS
Sbjct: 317  LLLNNPQVIKKAQAEIDNNLEQGRLINESDVNKLPYLHSIITETLRIYPAGPLLVPHESS 376

Query: 646  EECIVGGYNVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGR 467
            EECIVGGY VPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGR
Sbjct: 377  EECIVGGYKVPSGTMLLVNVWAIQQDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGR 436

Query: 466  RGCPGEGLAMRVVGLALGALIQCFDWERVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTA 287
            RGCPGEGLAMRVV LALGALIQCFDWERVGEEM+DMSEGPGLTLPKVHPL+AKCRPRSTA
Sbjct: 437  RGCPGEGLAMRVVALALGALIQCFDWERVGEEMVDMSEGPGLTLPKVHPLEAKCRPRSTA 496

Query: 286  LNFISQF 266
            LNFISQF
Sbjct: 497  LNFISQF 503


>gb|AGC29952.1| CYP81B57 [Sinopodophyllum hexandrum]
          Length = 507

 Score =  677 bits (1746), Expect = 0.0
 Identities = 329/462 (71%), Positives = 390/462 (84%), Gaps = 3/462 (0%)
 Frame = -3

Query: 1645 FKKPLHRTLAKLSDKYGHVLYLQFGARPVLVVSSPSAVEDCLAKNDIIFANRPHLLMGKI 1466
            FKKPL+RTL  +S++YG VLY QFG+RPVLVVSSPSA E+CL KNDI FANRP L++GK 
Sbjct: 46   FKKPLYRTLTNISNQYGPVLYFQFGSRPVLVVSSPSAAEECLTKNDIGFANRPRLMIGKH 105

Query: 1465 LGHDYTTLLWAPYGPNWRNLRKISSIELLSANRLQSYSDIRHAEVKSMVQRLSGQGCDYR 1286
             G++YTTL WA YG NWRNLR+IS++E+ S NR+Q +S+IR  EVKSMV+RL   G +Y 
Sbjct: 106  SGNNYTTLAWASYGQNWRNLRRISALEMFSPNRIQMFSEIRTDEVKSMVRRLVA-GYEYH 164

Query: 1285 TVEMKTMIFELTLNVMMTMIAGKRYFGGQVEELEQAKRFQEIVQGTLSISGASNIGDFLP 1106
             VEMK++ FELTLN+MM MIAGKRY+G  V +LEQA++F+EIV+ T  +SGASN+GDFLP
Sbjct: 165  KVEMKSVFFELTLNIMMRMIAGKRYYGEHVADLEQARKFKEIVEETFELSGASNVGDFLP 224

Query: 1105 ILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHRISRNGGTQ--NKEKNATLIDVLLSLQE 932
            +L WIG KGIEK++  LK KRD+FLQELI+E R  R       ++EK  TLIDVLLSLQE
Sbjct: 225  LLRWIGYKGIEKRLIRLKNKRDEFLQELIQERRKLRVNSKSFPSEEKKKTLIDVLLSLQE 284

Query: 931  TEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNTLEQGRL 752
            TEPEYYTDEMI G+IW+L AAGTDTSA TMEWAMSLLLNNP +IKKAQ+EID  +E GR 
Sbjct: 285  TEPEYYTDEMIRGLIWILFAAGTDTSAGTMEWAMSLLLNNPDLIKKAQSEIDLQIEPGRP 344

Query: 751  INESDVNKLPYLHSIINETLRIYPAGPLLVPHESSEECIVGGYNVPSGTMLLVNVWAIQQ 572
            I+ESD+NKLPYLH IINETLR+YPAGPLL+PHESSE+C +GGYN+P GTMLLVN+WAIQ 
Sbjct: 345  IDESDLNKLPYLHCIINETLRMYPAGPLLIPHESSEDCEIGGYNIPCGTMLLVNLWAIQN 404

Query: 571  DPNIWVEPTKFKPERFDGFE-GTRDGFKLMPFGSGRRGCPGEGLAMRVVGLALGALIQCF 395
            DPN+W EP KFKPERF+G++ G RDGFKLMPFGSGRRGCPGEGLA RVVGL L +L+QCF
Sbjct: 405  DPNLWKEPRKFKPERFEGYQGGVRDGFKLMPFGSGRRGCPGEGLAXRVVGLTLXSLLQCF 464

Query: 394  DWERVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTALNFISQ 269
            DW RVGEEM++MSEG GLTLPK+HPL+A CRPRST LNF+SQ
Sbjct: 465  DWHRVGEEMVEMSEGTGLTLPKLHPLEAHCRPRSTMLNFLSQ 506


>ref|XP_002283222.2| PREDICTED: isoflavone 2'-hydroxylase-like [Vitis vinifera]
          Length = 503

 Score =  604 bits (1557), Expect = e-170
 Identities = 296/462 (64%), Positives = 374/462 (80%), Gaps = 3/462 (0%)
 Frame = -3

Query: 1645 FKKPLHRTLAKLSDKYGHVLYLQFGARPVLVVSSPSAVEDCLAKNDIIFANRPHLLMGKI 1466
            FKKPLHRT AK+S++YG +L+++FG+RPV++VSSPSA E+C  KNDI+FANRP LL GK 
Sbjct: 45   FKKPLHRTFAKISNQYGPILFIRFGSRPVIIVSSPSAAEECFTKNDIVFANRPRLLAGKH 104

Query: 1465 LGHDYTTLLWAPYGPNWRNLRKISSIELLSANRLQSYSDIRHAEVKSMVQRL---SGQGC 1295
            LG++YTTL WAPYG +WRNLR+I+S+E+LS+NRLQ + DIR  EV++++ +L   S +G 
Sbjct: 105  LGYNYTTLTWAPYGQHWRNLRRIASLEILSSNRLQMFYDIRIDEVRALLCQLFRASSEG- 163

Query: 1294 DYRTVEMKTMIFELTLNVMMTMIAGKRYFGGQVEELEQAKRFQEIVQGTLSISGASNIGD 1115
             +  V+MK+M FELTLN MM MI+GKRY+G  V ELE+ ++F+EIV  T  +SGA+NI D
Sbjct: 164  QFSAVDMKSMFFELTLNNMMRMISGKRYYGDNVTELEETRKFREIVAETFELSGATNIVD 223

Query: 1114 FLPILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHRISRNGGTQNKEKNATLIDVLLSLQ 935
            F+P   WIG+ GIEKK+  L+ KRD F+Q LIEEHR  R+     + ++ T++DVLLSLQ
Sbjct: 224  FVPFSKWIGLNGIEKKLVILQGKRDGFMQNLIEEHRRMRS---PCEGRSKTMLDVLLSLQ 280

Query: 934  ETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNTLEQGR 755
            ETEPE YTD++I G++ V+L+AGTDTSA TMEWAMSLLLNNP+ ++KAQAEID+ L + R
Sbjct: 281  ETEPECYTDDIIRGMMQVMLSAGTDTSAGTMEWAMSLLLNNPEALEKAQAEIDSHLGKSR 340

Query: 754  LINESDVNKLPYLHSIINETLRIYPAGPLLVPHESSEECIVGGYNVPSGTMLLVNVWAIQ 575
            LI+E D+ +LPYL  II ETLR+YPA PLLVPHESSEEC VGG+ VPSGTMLLVN+WAIQ
Sbjct: 341  LIDELDIAELPYLRGIIKETLRMYPAAPLLVPHESSEECTVGGFRVPSGTMLLVNMWAIQ 400

Query: 574  QDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGLAMRVVGLALGALIQCF 395
             DP +W EP+KFKPERF G EG R+GF   PFG+GRRGCPGEGLAMRVVGLALG+LIQ F
Sbjct: 401  NDPMLWAEPSKFKPERFQGPEGQRNGFMFSPFGAGRRGCPGEGLAMRVVGLALGSLIQFF 460

Query: 394  DWERVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTALNFISQ 269
            +WERV EEM+DMSEG GLT+PK   L AKCRPR + ++ +SQ
Sbjct: 461  EWERVDEEMVDMSEGTGLTMPKAQSLVAKCRPRPSMVSLLSQ 502


>ref|XP_002510130.1| cytochrome P450, putative [Ricinus communis]
            gi|223550831|gb|EEF52317.1| cytochrome P450, putative
            [Ricinus communis]
          Length = 500

 Score =  603 bits (1556), Expect = e-170
 Identities = 294/459 (64%), Positives = 368/459 (80%), Gaps = 1/459 (0%)
 Frame = -3

Query: 1642 KKPLHRTLAKLSDKYGHVLYLQFGARPVLVVSSPSAVEDCLAKNDIIFANRPHLLMGKIL 1463
            KKPLHR+L+ LS++YG VL LQFG R VLVVSSPSA E+CL K D +FANRP LL GK +
Sbjct: 44   KKPLHRSLSTLSNRYGPVLLLQFGYRRVLVVSSPSAAEECLTKCDTVFANRPRLLAGKHI 103

Query: 1462 GHDYTTLLWAPYGPNWRNLRKISSIELLSANRLQSYSDIRHAEVKSMVQRLSGQGCDYRT 1283
            G++YT+L WAPYG  WRNLRK+S++E+LS++RLQ  S IR  EVK ++QRL     D R 
Sbjct: 104  GYNYTSLAWAPYGDLWRNLRKVSALEILSSHRLQLLSSIRRDEVKLLIQRLFRNNKDCRE 163

Query: 1282 -VEMKTMIFELTLNVMMTMIAGKRYFGGQVEELEQAKRFQEIVQGTLSISGASNIGDFLP 1106
             V+MK+  FEL LNVMM MIAGKRY+G  VE++E+AKRF+EIV+ T   +G SN+GDFLP
Sbjct: 164  KVDMKSAFFELMLNVMMRMIAGKRYYGENVEQVEEAKRFREIVRETFLAAGTSNMGDFLP 223

Query: 1105 ILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHRISRNGGTQNKEKNATLIDVLLSLQETE 926
            +++ +G  G EK++  L ++RD F+Q+LI+EHR  R     ++E+N T+I+VLLSLQE+E
Sbjct: 224  LVAVVG--GQEKRLKELGKRRDGFIQDLIDEHR-KRMAACSSEERNKTMIEVLLSLQESE 280

Query: 925  PEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNTLEQGRLIN 746
            PEYYTDE I  ++ VLLAAGTDTSA TMEWAMSLL+NNP+ +KKAQ EID+ +   RLIN
Sbjct: 281  PEYYTDETIKSLMLVLLAAGTDTSAATMEWAMSLLVNNPEALKKAQTEIDSVIGHDRLIN 340

Query: 745  ESDVNKLPYLHSIINETLRIYPAGPLLVPHESSEECIVGGYNVPSGTMLLVNVWAIQQDP 566
            ESD +KLPYL+ IINE +R+YPAGPLLVPHESSEEC +GGY VP+GTMLLVN+W+IQ DP
Sbjct: 341  ESDTSKLPYLNCIINEVMRMYPAGPLLVPHESSEECFIGGYRVPAGTMLLVNLWSIQNDP 400

Query: 565  NIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGLAMRVVGLALGALIQCFDWE 386
             +W EP  FKPERF+G EG RDGF+LMPFGSGRR CPGEGLA+R+VGL +G L+QCFDWE
Sbjct: 401  RVWEEPRNFKPERFEGCEGVRDGFRLMPFGSGRRSCPGEGLALRMVGLGIGTLLQCFDWE 460

Query: 385  RVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTALNFISQ 269
            RVG+EMIDM+EG GLT+PK  PL  +C PR + +N +SQ
Sbjct: 461  RVGKEMIDMTEGVGLTMPKAQPLVVQCSPRPSMVNLLSQ 499


>emb|CAN68429.1| hypothetical protein VITISV_012133 [Vitis vinifera]
          Length = 1001

 Score =  603 bits (1554), Expect = e-170
 Identities = 296/462 (64%), Positives = 373/462 (80%), Gaps = 3/462 (0%)
 Frame = -3

Query: 1645 FKKPLHRTLAKLSDKYGHVLYLQFGARPVLVVSSPSAVEDCLAKNDIIFANRPHLLMGKI 1466
            FKKPLHRT AK+S++YG +L+++FG+RPV++VSSPSA E+C  KNDI+FANRP LL GK 
Sbjct: 543  FKKPLHRTFAKISNQYGPILFIRFGSRPVIIVSSPSAAEECFTKNDIVFANRPRLLAGKH 602

Query: 1465 LGHDYTTLLWAPYGPNWRNLRKISSIELLSANRLQSYSDIRHAEVKSMVQRL---SGQGC 1295
            LG++YTTL WAPYG +WRNLR+I+S+E+LS+NRLQ + DIR  EV++++ +L   S +G 
Sbjct: 603  LGYNYTTLTWAPYGQHWRNLRRIASLEILSSNRLQMFYDIRIDEVRALLCQLFRASSEG- 661

Query: 1294 DYRTVEMKTMIFELTLNVMMTMIAGKRYFGGQVEELEQAKRFQEIVQGTLSISGASNIGD 1115
             +  V+MK+M FELTLN MM MI+GKRY+G  V ELE+ ++F+EIV  T  +SGA+NI D
Sbjct: 662  QFSAVDMKSMFFELTLNNMMRMISGKRYYGDNVTELEETRKFREIVAETFELSGATNIVD 721

Query: 1114 FLPILSWIGVKGIEKKVWNLKEKRDKFLQELIEEHRISRNGGTQNKEKNATLIDVLLSLQ 935
            F+P   WIG+ GIEKK+  L+ KRD F+Q LIEEHR  R+     + ++ T++DVLLSLQ
Sbjct: 722  FVPFSKWIGLNGIEKKLVILQGKRDGFMQNLIEEHRRMRS---PCEXRSKTMLDVLLSLQ 778

Query: 934  ETEPEYYTDEMILGIIWVLLAAGTDTSAVTMEWAMSLLLNNPQVIKKAQAEIDNTLEQGR 755
            ETEPE YTD++I G++ V+L+AGTDTSA TMEWAMSLLLNNP+ ++KAQAEID+ L + R
Sbjct: 779  ETEPECYTDDIIRGMMQVMLSAGTDTSAGTMEWAMSLLLNNPEALEKAQAEIDSHLGKSR 838

Query: 754  LINESDVNKLPYLHSIINETLRIYPAGPLLVPHESSEECIVGGYNVPSGTMLLVNVWAIQ 575
            LI+E D+  LPYL  II ETLR+YPA PLLVPHESSEEC VGG+ VPSGTMLLVN+WAIQ
Sbjct: 839  LIDELDIAXLPYLRGIIMETLRMYPAAPLLVPHESSEECTVGGFRVPSGTMLLVNMWAIQ 898

Query: 574  QDPNIWVEPTKFKPERFDGFEGTRDGFKLMPFGSGRRGCPGEGLAMRVVGLALGALIQCF 395
             DP +W EP+KFKPERF G EG R+GF   PFG+GRRGCPGEGLAMRVVGLALG+LIQ F
Sbjct: 899  NDPMLWAEPSKFKPERFQGPEGQRNGFMFSPFGAGRRGCPGEGLAMRVVGLALGSLIQFF 958

Query: 394  DWERVGEEMIDMSEGPGLTLPKVHPLKAKCRPRSTALNFISQ 269
            +WERV EEM+DMSEG GLT+PK   L AKCRPR + ++ +SQ
Sbjct: 959  EWERVDEEMVDMSEGTGLTMPKAQSLVAKCRPRPSMVSLLSQ 1000