BLASTX nr result
ID: Coptis24_contig00004068
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Coptis24_contig00004068
(2087 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 cataly... 503 0.0
ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 cataly... 497 0.0
ref|XP_003610389.1| NEDD8-activating enzyme E1 catalytic subunit... 482 0.0
ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [R... 482 e-179
ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|2... 481 e-179
>ref|XP_004134149.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit-like [Cucumis
sativus] gi|449515377|ref|XP_004164726.1| PREDICTED:
NEDD8-activating enzyme E1 catalytic subunit-like
[Cucumis sativus]
Length = 449
Score = 503 bits (1294), Expect(3) = 0.0
Identities = 244/327 (74%), Positives = 271/327 (82%)
Frame = +3
Query: 819 SYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPXXXXXXXXXXXX 998
SYINSVACSFLEYDSDD P EETIKPMVDGGTEGFKGHARVILPGVTP
Sbjct: 146 SYINSVACSFLEYDSDDNPLEETIKPMVDGGTEGFKGHARVILPGVTP------------ 193
Query: 999 XXXXXXXXXXXCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFD 1178
CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK+FD
Sbjct: 194 -----------CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKSFD 242
Query: 1179 ADDPEHMQWIYSEALKRAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKI 1358
DD EHM+W+YSEALKRAELFGIPGVTYSLTQGVVK CALE LKI
Sbjct: 243 PDDSEHMKWVYSEALKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKI 302
Query: 1359 VTACSRIMSNYLTYNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEH 1538
V+ CS+ +SNYLTYNG EGLHTK+TEFV+DKDCLVCGPG+L+EL++SITLQKF++L+ +H
Sbjct: 303 VSGCSKTLSNYLTYNGAEGLHTKVTEFVKDKDCLVCGPGVLIELDSSITLQKFIDLLEDH 362
Query: 1539 PKLLLSKPSVTYQGKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDK 1718
PKLLLSK SVT++GK LYMQAPPVLEEMTRSNL +PLFDLM K+ KDV+HVTG+ +K+DK
Sbjct: 363 PKLLLSKASVTHRGKNLYMQAPPVLEEMTRSNLGVPLFDLMGKVLKDVVHVTGVASKNDK 422
Query: 1719 KTSCLRKLRIVFKGVDGITDVDMASGA 1799
KTSCLRKLR+ FKGVDG+TD+D ASGA
Sbjct: 423 KTSCLRKLRVAFKGVDGVTDMDTASGA 449
Score = 117 bits (292), Expect(3) = 0.0
Identities = 55/63 (87%), Positives = 62/63 (98%)
Frame = +1
Query: 589 NRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSI 768
NRQFLFRL+DVGKPKAEVAAKRVMERVSGVNI+PHFCRIEDK+++FY +F+IIALGLDSI
Sbjct: 83 NRQFLFRLEDVGKPKAEVAAKRVMERVSGVNILPHFCRIEDKEIEFYNDFHIIALGLDSI 142
Query: 769 EAR 777
EAR
Sbjct: 143 EAR 145
Score = 82.0 bits (201), Expect(3) = 0.0
Identities = 49/113 (43%), Positives = 56/113 (49%)
Frame = +3
Query: 222 MTNTGKWRDLDKLLSRPGNLVNPGFEPSPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 401
+ + + RDLDKLL RPGNL+ FEP +
Sbjct: 5 VVQSSRSRDLDKLLLRPGNLIGANFEPGSQLRDDLQ------------------------ 40
Query: 402 XXXXXXXXXXXXGEYTKVLVVGAGGLGCELLKDLALSGFTNLHVIDMDTIEVT 560
+Y KVLV+GAGGLGCELLKDLALSGF NL VIDMD IEVT
Sbjct: 41 -------------QYVKVLVIGAGGLGCELLKDLALSGFRNLEVIDMDRIEVT 80
>ref|XP_002280928.1| PREDICTED: NEDD8-activating enzyme E1 catalytic subunit [Vitis
vinifera] gi|297736997|emb|CBI26198.3| unnamed protein
product [Vitis vinifera]
Length = 449
Score = 497 bits (1280), Expect(3) = 0.0
Identities = 239/327 (73%), Positives = 267/327 (81%)
Frame = +3
Query: 819 SYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPXXXXXXXXXXXX 998
SYIN+VACSFLEYDSDDKP EETIKPMVDGGTEGFKGHARVI+PGVTP
Sbjct: 146 SYINAVACSFLEYDSDDKPLEETIKPMVDGGTEGFKGHARVIIPGVTP------------ 193
Query: 999 XXXXXXXXXXXCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFD 1178
CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFD
Sbjct: 194 -----------CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFD 242
Query: 1179 ADDPEHMQWIYSEALKRAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKI 1358
DDPEHM+W+YSEA+KRAELFGIPGVTYSLTQGVVK CALE LKI
Sbjct: 243 PDDPEHMKWVYSEAVKRAELFGIPGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKI 302
Query: 1359 VTACSRIMSNYLTYNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEH 1538
V+ CS+ +SNYLTYNG EGLHTK+TEFV+D DCL+CGPG+L+EL+TS+TLQKFM+ + EH
Sbjct: 303 VSGCSKTLSNYLTYNGAEGLHTKVTEFVKDMDCLICGPGVLIELDTSVTLQKFMDQLQEH 362
Query: 1539 PKLLLSKPSVTYQGKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDK 1718
PKLLLSK SV ++ K LY+QAPPVLEE+TRSNL LPLFDLM K+ KD++HVTG T K+DK
Sbjct: 363 PKLLLSKASVKHREKNLYIQAPPVLEELTRSNLNLPLFDLMGKIRKDIVHVTGSTTKNDK 422
Query: 1719 KTSCLRKLRIVFKGVDGITDVDMASGA 1799
KTSCLRKLR+VFKG+DG TD+D A GA
Sbjct: 423 KTSCLRKLRVVFKGIDGATDMDTAGGA 449
Score = 122 bits (305), Expect(3) = 0.0
Identities = 59/63 (93%), Positives = 61/63 (96%)
Frame = +1
Query: 589 NRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSI 768
NRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKD++FY FNIIALGLDSI
Sbjct: 83 NRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDIEFYNHFNIIALGLDSI 142
Query: 769 EAR 777
EAR
Sbjct: 143 EAR 145
Score = 81.3 bits (199), Expect(3) = 0.0
Identities = 50/106 (47%), Positives = 54/106 (50%)
Frame = +3
Query: 243 RDLDKLLSRPGNLVNPGFEPSPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 422
RDLDKLL RPG+LV P FEP PE
Sbjct: 12 RDLDKLLLRPGHLVGPTFEPGPELRDDLR------------------------------- 40
Query: 423 XXXXXGEYTKVLVVGAGGLGCELLKDLALSGFTNLHVIDMDTIEVT 560
++ VLVVGAGGLGCELLKDLALSGF NL VIDMD IEV+
Sbjct: 41 ------KFATVLVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVS 80
>ref|XP_003610389.1| NEDD8-activating enzyme E1 catalytic subunit [Medicago truncatula]
gi|355511444|gb|AES92586.1| NEDD8-activating enzyme E1
catalytic subunit [Medicago truncatula]
Length = 454
Score = 482 bits (1240), Expect(3) = 0.0
Identities = 233/327 (71%), Positives = 261/327 (79%)
Frame = +3
Query: 819 SYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPXXXXXXXXXXXX 998
SYIN+VACSFLEYDSDD P EETIKPMVDGGTEGFKGHARVI+PG+TP
Sbjct: 151 SYINTVACSFLEYDSDDNPREETIKPMVDGGTEGFKGHARVIMPGITP------------ 198
Query: 999 XXXXXXXXXXXCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFD 1178
CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVH G FD
Sbjct: 199 -----------CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHRGVPFD 247
Query: 1179 ADDPEHMQWIYSEALKRAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKI 1358
D+PE+M+W+Y EA+KRAELFGIPGVTYS TQGVVK CALE LKI
Sbjct: 248 PDNPENMKWVYDEAVKRAELFGIPGVTYSFTQGVVKNIIPAIASTNAIISAACALETLKI 307
Query: 1359 VTACSRIMSNYLTYNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEH 1538
T CS+ +SNYLTYNG EGLHTK+TEF RDKDCLVCGPGI +EL+ SITLQKFM+L+ EH
Sbjct: 308 ATECSKTLSNYLTYNGSEGLHTKVTEFERDKDCLVCGPGIRIELDPSITLQKFMDLLEEH 367
Query: 1539 PKLLLSKPSVTYQGKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDK 1718
PKL LSK SVT++GK LYMQAPPVLEEMTRSNL L LF+LM K+ KD++HV GMT+K+D+
Sbjct: 368 PKLRLSKASVTHRGKNLYMQAPPVLEEMTRSNLTLSLFNLMGKLPKDIVHVNGMTSKNDQ 427
Query: 1719 KTSCLRKLRIVFKGVDGITDVDMASGA 1799
KTSCLRKLR+VFKG+DG+TD+D A GA
Sbjct: 428 KTSCLRKLRVVFKGIDGVTDMDTAGGA 454
Score = 117 bits (294), Expect(3) = 0.0
Identities = 55/63 (87%), Positives = 62/63 (98%)
Frame = +1
Query: 589 NRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSI 768
NRQFLFRL+DVGKPKAEVAAKRVMER+SGVNIVPHFCRIEDK+++FY +F+IIALGLDSI
Sbjct: 88 NRQFLFRLEDVGKPKAEVAAKRVMERISGVNIVPHFCRIEDKEIEFYNDFSIIALGLDSI 147
Query: 769 EAR 777
EAR
Sbjct: 148 EAR 150
Score = 85.1 bits (209), Expect(3) = 0.0
Identities = 53/106 (50%), Positives = 54/106 (50%)
Frame = +3
Query: 243 RDLDKLLSRPGNLVNPGFEPSPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 422
RDLDKLL RPGNLV P FEP E
Sbjct: 17 RDLDKLLLRPGNLVGPRFEPGAELRDDLQ------------------------------- 45
Query: 423 XXXXXGEYTKVLVVGAGGLGCELLKDLALSGFTNLHVIDMDTIEVT 560
E+ KVLVVGAGGLGCELLKDLALSGF NL VIDMD IEVT
Sbjct: 46 ------EFAKVLVVGAGGLGCELLKDLALSGFRNLDVIDMDRIEVT 85
>ref|XP_002517184.1| ubiquitin-activating enzyme E1c, putative [Ricinus communis]
gi|223543819|gb|EEF45347.1| ubiquitin-activating enzyme
E1c, putative [Ricinus communis]
Length = 449
Score = 482 bits (1240), Expect(3) = e-179
Identities = 227/327 (69%), Positives = 266/327 (81%)
Frame = +3
Query: 819 SYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPXXXXXXXXXXXX 998
SYIN+VACSFLEYDS+D P ++T+KPMVDGGTEGFKGHARVI+PG+TP
Sbjct: 146 SYINNVACSFLEYDSEDNPRDDTMKPMVDGGTEGFKGHARVIMPGITP------------ 193
Query: 999 XXXXXXXXXXXCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFD 1178
CFECTIWLFPPQVKFPLCTLAETPRT AHCIEYAHLIKW+EVHSGKAFD
Sbjct: 194 -----------CFECTIWLFPPQVKFPLCTLAETPRTPAHCIEYAHLIKWNEVHSGKAFD 242
Query: 1179 ADDPEHMQWIYSEALKRAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKI 1358
DDPEHM+W+Y+EA+KRAELFGI GVTYSLTQGVVK CALE LKI
Sbjct: 243 PDDPEHMKWVYTEAVKRAELFGIQGVTYSLTQGVVKNIIPAIASTNAIISAACALETLKI 302
Query: 1359 VTACSRIMSNYLTYNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEH 1538
+ CS+ +SNYLTYNG+EGLH K+TEFV+DKDCL CGPG+L+EL+TS+ LQKF++L+ EH
Sbjct: 303 ASGCSKTLSNYLTYNGVEGLHIKVTEFVKDKDCLACGPGVLIELDTSVALQKFIDLLEEH 362
Query: 1539 PKLLLSKPSVTYQGKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDK 1718
PKL LS+ SVT++GK LYMQAPPVLEEMTRSNL LPLF+LM K+ KD++HVTGMT ++ K
Sbjct: 363 PKLFLSRASVTHRGKNLYMQAPPVLEEMTRSNLSLPLFELMGKVPKDIVHVTGMTGQNGK 422
Query: 1719 KTSCLRKLRIVFKGVDGITDVDMASGA 1799
KTSC+RKLR+VFKG++GITD+DMA GA
Sbjct: 423 KTSCVRKLRVVFKGMEGITDMDMAGGA 449
Score = 119 bits (297), Expect(3) = e-179
Identities = 57/63 (90%), Positives = 61/63 (96%)
Frame = +1
Query: 589 NRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSI 768
NRQFLFRL+DVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDK+LDFY +F+II LGLDSI
Sbjct: 83 NRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKELDFYNDFSIIVLGLDSI 142
Query: 769 EAR 777
EAR
Sbjct: 143 EAR 145
Score = 80.5 bits (197), Expect(3) = e-179
Identities = 49/106 (46%), Positives = 54/106 (50%)
Frame = +3
Query: 243 RDLDKLLSRPGNLVNPGFEPSPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 422
RDLDKLL RPGNLV P F+P +
Sbjct: 12 RDLDKLLLRPGNLVAPTFDPGVQLRDDLQ------------------------------- 40
Query: 423 XXXXXGEYTKVLVVGAGGLGCELLKDLALSGFTNLHVIDMDTIEVT 560
EY ++LVVGAGGLGCELLKDLALSGF NL VIDMD IEV+
Sbjct: 41 ------EYVRILVVGAGGLGCELLKDLALSGFKNLEVIDMDRIEVS 80
>ref|XP_002311839.1| predicted protein [Populus trichocarpa] gi|222851659|gb|EEE89206.1|
predicted protein [Populus trichocarpa]
Length = 449
Score = 481 bits (1238), Expect(3) = e-179
Identities = 230/327 (70%), Positives = 260/327 (79%)
Frame = +3
Query: 819 SYINSVACSFLEYDSDDKPSEETIKPMVDGGTEGFKGHARVILPGVTPXXXXXXXXXXXX 998
SYIN+VAC FLEYDSDD P EET+KPMVDGGTEGFKGHARVI+PG TP
Sbjct: 146 SYINAVACGFLEYDSDDNPIEETVKPMVDGGTEGFKGHARVIIPGSTP------------ 193
Query: 999 XXXXXXXXXXXCFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKAFD 1178
CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGK FD
Sbjct: 194 -----------CFECTIWLFPPQVKFPLCTLAETPRTAAHCIEYAHLIKWDEVHSGKTFD 242
Query: 1179 ADDPEHMQWIYSEALKRAELFGIPGVTYSLTQGVVKXXXXXXXXXXXXXXXXCALEALKI 1358
DDPEHM+W+Y+EA+KRAELFGI GVTYSLTQGVVK C LE LK+
Sbjct: 243 PDDPEHMKWVYTEAVKRAELFGIQGVTYSLTQGVVKNIIPAIASTNAIISAACTLETLKL 302
Query: 1359 VTACSRIMSNYLTYNGLEGLHTKMTEFVRDKDCLVCGPGILLELETSITLQKFMELVGEH 1538
+ CS+ +SNYLTYNG+EGLH K+TEFV+DKDCLVCGPG+LLEL+TS+TLQKF++++ EH
Sbjct: 303 ASGCSKTLSNYLTYNGVEGLHIKVTEFVKDKDCLVCGPGVLLELDTSVTLQKFIDMLEEH 362
Query: 1539 PKLLLSKPSVTYQGKTLYMQAPPVLEEMTRSNLKLPLFDLMDKMSKDVIHVTGMTNKDDK 1718
PKLLLSK SV ++ LYMQAPPVLEEMTRSNL LPLF+LM K KD++HVTG T+KDDK
Sbjct: 363 PKLLLSKASVRHRATNLYMQAPPVLEEMTRSNLNLPLFELMGKFPKDIVHVTGTTSKDDK 422
Query: 1719 KTSCLRKLRIVFKGVDGITDVDMASGA 1799
KTSCLRKL +VFKG D +TD+DMA GA
Sbjct: 423 KTSCLRKLCLVFKGADAVTDLDMAVGA 449
Score = 117 bits (292), Expect(3) = e-179
Identities = 55/63 (87%), Positives = 61/63 (96%)
Frame = +1
Query: 589 NRQFLFRLQDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKDLDFYREFNIIALGLDSI 768
NRQFLFRL+DVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDK++DFY++F II LGLDS+
Sbjct: 83 NRQFLFRLEDVGKPKAEVAAKRVMERVSGVNIVPHFCRIEDKEIDFYKDFIIIVLGLDSV 142
Query: 769 EAR 777
EAR
Sbjct: 143 EAR 145
Score = 83.2 bits (204), Expect(3) = e-179
Identities = 50/106 (47%), Positives = 54/106 (50%)
Frame = +3
Query: 243 RDLDKLLSRPGNLVNPGFEPSPEXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 422
RDLDKLL RPGNLV P FEP +
Sbjct: 12 RDLDKLLLRPGNLVAPTFEPGAQLRDDLQ------------------------------- 40
Query: 423 XXXXXGEYTKVLVVGAGGLGCELLKDLALSGFTNLHVIDMDTIEVT 560
EY ++LV+GAGGLGCELLKDLALSGF NL VIDMD IEVT
Sbjct: 41 ------EYARILVIGAGGLGCELLKDLALSGFKNLEVIDMDRIEVT 80